RESUMO
The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of Tizi-Ouzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain extracts was carried out in a 96-well microplate and on a biomaterial (catheter). HPLC analysis revealed the presence of 10 bioactive compounds. The main phenolic compounds identified in the three extracts were p-coumaric acid, ellagic acid, and Kaempferol. This study was able to demonstrate that the extracts of P. nigra and P. alba buds have interesting antimicrobial properties, with diameters ranging from 6.6 to 21.3 mm. In addition, extracts of P. nigra exhibited antibiofilm effects greater than 70%. Our results provide evidence for the antimicrobial and antibiofilm potential of bud extracts from both poplar species. Thus, these results will pave the way for further research on these two plants.
Assuntos
Extratos Vegetais , Biofilmes/classificação , Populus/anatomia & histologia , Anti-Infecciosos/farmacocinética , Cromatografia Líquida de Alta Pressão/instrumentação , Argélia/etnologia , Compostos Fenólicos/análise , Polifenóis/farmacocinéticaRESUMO
Propolis is a resinous hive product collected by honeybees from plant source. The composition of the propolis depends upon the time, vegetation and the area of collection. Propolis produced by africanized Apis mellifera from south Brazil, Argentine and Uruguay have been investigated using chromatographic techniques. In accordance with results RP-HPTLC, RP-HPLC, and GC-MS, propolis from south Brazil were classified IN 5 groups. Based on chemical evidence Populus alba was shown to be the main propolis source of group 3 from south Brazil, Argentine and Uruguay.
A própolis é uma substância resinosa coletada pelas abelhas de diversas partes da planta. Sua composição depende da época, vegetação e área de coleta. Própolis produzida por abelhas Apis mellifera africanizada do sul do Brasil, Argentina e Uruguai foram investigadas por meio de técnicas cromatográficas. De acordo com os resultados obtidos por CCDAE-FR, CLAE-FR e CG-EM, as própolis do sul do Brasil foram classificadas em 5 grupos distintos. Com base nas evidências fitoquímicas, Populus alba foi identificada como a principal fonte da própolis do grupo 3 do sul do Brasil, Argentina e Uruguai.
RESUMO
Propolis is a resinous hive product collected by honeybees from plant source. The composition of the propolis depends upon the time, vegetation and the area of collection. Propolis produced by africanized Apis mellifera from south Brazil, Argentine and Uruguay have been investigated using chromatographic techniques. In accordance with results RP-HPTLC, RP-HPLC, and GC-MS, propolis from south Brazil were classified IN 5 groups. Based on chemical evidence Populus alba was shown to be the main propolis source of group 3 from south Brazil, Argentine and Uruguay.
A própolis é uma substância resinosa coletada pelas abelhas de diversas partes da planta. Sua composição depende da época, vegetação e área de coleta. Própolis produzida por abelhas Apis mellifera africanizada do sul do Brasil, Argentina e Uruguai foram investigadas por meio de técnicas cromatográficas. De acordo com os resultados obtidos por CCDAE-FR, CLAE-FR e CG-EM, as própolis do sul do Brasil foram classificadas em 5 grupos distintos. Com base nas evidências fitoquímicas, Populus alba foi identificada como a principal fonte da própolis do grupo 3 do sul do Brasil, Argentina e Uruguai.