Comparative transcriptome analyses on terpenoids metabolism in field- and mountain-cultivated ginseng roots.

BACKGROUND: There exist differences in morphological traits and phytochemical compositions between field- and mountain-cultivated Panax ginseng (FCG and MCG), which might be attributed to variations of terpenoids metabolism adapting to different growth conditions. The present work aims to uncover these variations. RESULTS: Among 26,648 differentially expressed genes, 496 genes distributed in seven dominant terpenoids pathways were identified. Diterpenoids and triterpenoids biosynthesis genes were significantly higher-expressed in FCG root. Conversely, biosynthesis of carotenoids was significantly more active in MCG root. Additionally, terpenoids backbones, monoterpenoids, sesquiterpenoids, and terpenoid-quinones biosyntheses were neither obviously inclined. Our determination also revealed that there were more gibberellins and steroids accumulated in FCG root which might be responsible for its quick vegetative growth, and enriched abscisic acid and germacrenes as well as protopanaxatriol-type ginsenosides might be major causes of enhanced stress-resistance in MCG root. CONCLUSIONS: The study firstly provided an overview of terpenoids metabolism in roots of FCG and MCG in elucidating the underlying mechanisms for their different morphological appearances and phytochemical compositions.

Research advances in terpenoids biosynthesis pathway of tomatoes / 国际生物医学工程杂志

The metabolic activity of terpenoids not only have a grant effect on normal growth of tomato plants,but also have a great influence on the quality and colour of the fruits.Most terpenoids belong to the carotenoids,which are synthesized through terpenoid pathway.Three acety-CoAs are condensated into one mevalonate (MVA),which will be phosphorylated into diphosphate,then turn into isopentenyl diphosphate (IPP) through decarboxylation.IPP will be isomerased into dimethylallyl diphosphate (DMAPP) by IPP isomerase (IPPI).Geranyl Geranyl diphosphate synthase (GGPPS) catalyze the reaction for DMAPP and IPP.One DMAPP and three IPP molecules condensedinto one GGPP.Two GGPP polymerize into one phytoene by phytoene synthase (PSY).Phytoene desaturase (PDS) catalyze the phytoene into ζ-carotene,which catalyzed into lycopene by ζ-carotene desaturase (ZDS).Lycopene βcyclase (LCYb) and lycopene ε cyclase (LCYe) catalyze the lycopene turn into α-carotene and β-carotene after cyclization reaction.The lutein will be combined after two step of hydroxylation.The latest research advances in terpeniods biosynthesis pathway are summarized in this review and foreground of the field is prospected.