Influence of perfluorooctanoic acid on alkaline anaerobic fermentation of waste activated sludge: Perspective from volatile fatty acids production and sludge reduction.

Alkaline anaerobic fermentation is an effective approach for resource utilization and reduction of waste activated sludge (WAS). Perfluorooctanoic acid (PFOA) is widespread in WAS, however, its potential impact on alkaline anaerobic fermentation of WAS remains largely unknown. Hence, this study focused on investigating the influence of PFOA on volatile fatty acids (VFAs) production and sludge reduction during alkaline anaerobic fermentation (pH = 10 ± 0.1), as well as the critical mechanisms. Results demonstrated that low PFOA concentration (5 mg/kg-TS) raised VFAs yield to 109.37%, while high levels of PFOA (25 and 50 mg/kg-TS) remarkably decreased VFAs production to 89.55% and 80.44% of the control. Mechanism exploration revealed that PFOA facilitated the solubilization process, and low PFOA level enhanced the accumulation of VFAs via increased bioavailable substrates and the activities of enzymes and microorganisms. On the contrary, high levels of PFOA were substantial biotoxicity, inducing excessive ROS production, causing oxidative damage, and reducing enzyme activity and functional microbial abundance, thereby decreasing VFAs production. Additionally, further analysis of sludge physicochemical properties confirmed that the effect of PFOA on WAS reduction exhibited the same trend as VFAs production. This work provides a basis for PFOA environmental risk assessment and WAS resource utilization.

Membrane electrolysis distillation for volatile fatty acids extraction from pH-neutral fermented wastewater.

Volatile fatty acids (VFAs) serve as building blocks for a wide range of chemicals, but it is difficult to extract VFAs from pH-neutral wastewater using evaporation methods because of the ionized form. This study presents a new membrane electrolysis distillation (MED) process that extracts VFAs from such fermentation solutions. MED uniquely integrates pH regulation and joule heating to facilitate the efficient evaporation of VFAs. This integration occurs alongside a hydrophobic membrane that ensures effective gas-liquid phase separation. Operating solely on electricity, MED achieved an acid flux rate of 12.03 g/m2/h at 6V. In contrast, the control results without the joule heating or pH swing only obtained a 0.23 g/m2/h and 0.32 g/m2/h flux, respectively. In addition, a physicochemical model was developed to assess the impacts of temperature on membrane surface pH. This system enhances resource recovery from waste streams and helps achieve a circular carbon economy.

Alleviation of volatile fatty acids inhibition in anaerobic digestion of swine manure with nano-bubble water supplementation.

Nano-bubble water (NBW) was applied to anaerobic digestion (AD) to alleviate volatile fatty acids (VFAs) inhibition, improve the buffering capacity and CH4 production in this work. Results indicated that NBW accelerated the consumption of VFAs and prevented inhibition due to VFAs accumulation. Additionally, NBW facilitated a rapid increase in partial alkalinity (PA) and total alkalinity (TA) as well as a corresponding rapid decrease in intermediate alkalinity (IA)/PA and VFA/TA, thereby improving buffering capacity and alleviating VFAs inhibition. Moreover, CH4 production improved by more than 12.2% by NBW. Similarly, the activities of the extracellular hydrolases and coenzyme F420 increased. Besides, NBW increased the abundance of microbial community and strengthened the metabolic pathways of hydrogenotrophic methanogens, which could be the intrinsic mechanism by which NBW alleviated VFAs inhibition, improved system stability, and increased CH4 production. This study demonstrates that NBW supplementation can be an effective method for mitigating frequent VFAs inhibition.

The Impacts of Cellulose on Volatile Fatty Acid Production and the Microbial Community in Anaerobic Fermentation of Sludge at High and Medium Temperatures.

During large-scale sewage treatment, a large amount of excessive sludge is produced, which will cause serious pollution in the environment. In recent years, anaerobic digestion technology has been widely promoted because it can achieve better sludge reduction, and the products and byproducts after anaerobic digestion can be fully utilized as resources. In this study, cellulose was added as the co-fermentation substrate during the fermentation process at 30 ℃ and 50 ℃ to enhance the production of VFAs. The result indicated that cellulose could significantly increase the yield of VFAs in both 30 ℃ and 50 ℃. Meanwhile, COD and reducing sugar generation in the fermentation process were also measure. Analysis of the microbial community structure at the class and genus levels revealed that the proportion of several genus closely related with cellulose degradation such as Cellvibrio, Fibrobacter, and Sporocytophaga were significantly increased with the addition of cellulose. Co-fermentation was recognized as an economic and environmental friendly strategy for sludge and other solid waste treatment. The analysis of the effect of cellulose as a substrate on the production of VFAs at high and medium temperatures is highly important for exploring ways to increase the production of VFAs in anaerobic fermentation.

Effects of the Interaction between Rumen Microbiota Density-VFAs-Hepatic Gluconeogenesis on the Adaptability of Tibetan Sheep to Plateau.

During the adaptive evolution of animals, the host and its gut microbiota co-adapt to different elevations. Currently, there are few reports on the rumen microbiota-hepato-intestinal axis of Tibetan sheep at different altitudes. Therefore, the purpose of this study was to explore the regulatory effect of rumen microorganism-volatile fatty acids (VFAs)-VFAs transporter gene interactions on the key enzymes and genes related to gluconeogenesis in Tibetan sheep. The rumen fermentation parameters, rumen microbial densities, liver gluconeogenesis activity and related genes were determined and analyzed using gas chromatography, RT-qPCR and other research methods. Correlation analysis revealed a reciprocal relationship among rumen microflora-VFAs-hepatic gluconeogenesis in Tibetan sheep at different altitudes. Among the microbiota, Ruminococcus flavefaciens (R. flavefaciens), Ruminococcus albus (R. albus), Fibrobactersuccinogenes and Ruminobacter amylophilus (R. amylophilus) were significantly correlated with propionic acid (p < 0.05), while propionic acid was significantly correlated with the transport genes monocarboxylate transporter 4 (MCT4) and anion exchanger 2 (AE2) (p < 0.05). Propionic acid was significantly correlated with key enzymes such as pyruvate carboxylase, phosphoenolpyruvic acid carboxylase and glucose (Glu) in the gluconeogenesis pathway (p < 0.05). Additionally, the expressions of these genes were significantly correlated with those of the related genes, namely, forkhead box protein O1 (FOXO1) and mitochondrial phosphoenolpyruvate carboxykinase 2 (PCK2) (p < 0.05). The results showed that rumen microbiota densities differed at different altitudes, and the metabolically produced VFA contents differed, which led to adaptive changes in the key enzyme activities of gluconeogenesis and the expressions of related genes.

Higher thermal remediation temperature facilitates the sequential bioaugmented reductive dechlorination.

The coupling of thermal remediation with microbial reductive dechlorination (MRD) has shown promising potential for the cleanup of chlorinated solvent contaminated sites. In this study, thermal treatment and bioaugmentation were applied in series, where prior higher thermal remediation temperature led to improved TCE dechlorination performance with both better organohalide-respiring bacteria (OHRB) colonization and electron donor availability. The 60 °C was found to be a key temperature point where the promotion effect became obvious. Amplicon sequencing and co-occurrence network analysis demonstrated that temperature was a more dominating factor than bioaugmentation that impacted microbial community structure. Higher temperature of prior thermal treatment resulted in the decrease of richness, diversity of indigenous microbial communities, and simplified the network structure, which benefited the build-up of newcoming microorganisms during bioaugmentation. Thus, the abundance of Desulfitobacterium increased from 0.11 % (25 °C) to 3.10 % (90 °C). Meanwhile, released volatile fatty acids (VFAs) during thermal remediation functioned as electron donors and boosted MRD. Our results provided temperature-specific information on synergistic effect of sequential thermal remediation and bioaugmentation, which contributed to better implementation of the coupled technologies in chloroethene-impacted sites.

Time-dependent interference of surfactants and CeO2/Fe2O3 nanoparticles co-occurrence on the volatile fatty acids biosynthesis during semi-continuous sludge fermentation.

Various exogenous contaminants typically coexist in waste activated sludge (WAS), and the long-term impacts of these co-occurring contaminants on WAS anaerobic fermentation and associated mechanisms remain largely unknown. This study reveals that the co-occurrence of surfactants and nanoparticles (NPs, i.e., Fe2O3 and CeO2, frequently detected in sludge) exhibited time-dependent impacts on the volatile fatty acids (VFAs) biosynthesis. Surfactants triggered WAS decomposition and enhanced NPs dispersion, leading to increased exposure of functional anaerobes to NPs toxicity, negatively affecting them. Consequently, key fermentation processes, acidogenic bacterial abundance, and metabolic functions were inhibited in co-occurrence reactors compared to those containing only surfactants in the early stage (before 56 d). Surprisingly, the fermentation systems containing surfactants collapsed subsequently, with VFAs yield at 72 d decreasing by 48.59-71.27 % compared to 56 d. The keystone microbes (i.e., Acidobacteria (16 d) vs Patescibacteria (56 d)) were reshaped, and metabolic traits (i.e., proB involved in intracellular metabolism) were downregulated by 0.05-78.02 % due to reduced microbial adaptive capacity (i.e., quorum sensing (QS)). Partial least squares path modeling (PLS-PM) analysis suggests that the microbial community was the predominant factor influencing VFAs generation. This study provides new insights into the long-term effects of co-contaminants on the biological treatment of WAS.

Unraveling the effects and mechanisms of microplastics on anaerobic fermentation: Exploring microbial communities and metabolic pathways.

To investigate the effects of microplastics (MPs) on hydrolysis, acidification and microbial characteristics during waste activated sludge (WAS) anaerobic fermentation process, five different kinds of MPs were added into the WAS fermentation system and results indicated that, compared to the control group, the addition of polyvinyl chloride (PVC)-MPs exhibited the least inhibition on volatile fatty acids (VFAs), reducing them by 13.49 %. Conversely, polyethylene (PE)-MPs resulted in the greatest inhibition, with a reduction of 29.57 %. MPs, while accelerated the dissolution of WAS that evidenced by an increase of lactate dehydrogenase (LDH) release, concurrently inhibited the activities of relevant hydrolytic enzymes (α-Glucosidase, protease). For microbial mechanisms, MPs addition affected the proliferation of key microorganisms (norank_f_Bacteroidetes_vadinHA17, Ottowia, and Propioniclava) and reduced the abundance of genes associated with hydrolysis and acidification (pfkb, gpmI, ilvE, and aces). Additionally, MPs decreased the levels of key hydrolytic and acidogenic enzymes to inhibit hydrolysis and acidification processes. This research provides a basis for understanding and unveils impact mechanisms of the impact of MPs on sludge anaerobic fermentation.

Biological bromate reduction coupled with in situ gas fermentation in H2/CO2-based membrane biofilm reactor.

Bromate, a carcinogenic contaminant generated in water disinfection, presents a pressing environmental concern. While biological bromate reduction is an effective remediation approach, its implementation often necessitates the addition of organics, incurring high operational costs. This study demonstrated the efficient biological bromate reduction using H2/CO2 mixture as the feedstock. A membrane biofilm reactor (MBfR) was used for the efficient delivery of gases. Long-term reactor operation showed a high-level bromate removal efficiency of above 95 %, yielding harmless bromide as the final product. Corresponding to the short hydraulic retention time of 0.25 d, a high bromate removal rate of 4 mg Br/L/d was achieved. During the long-term operation, in situ production of volatile fatty acids (VFAs) by gas fermentation was observed, which can be regulated by controlling the gas flow. Three sets of in situ batch tests and two groups of ex situ batch tests jointly unravelled the mechanisms underpinning the efficient bromate removal, showing that the microbial bromate reduction was primarily driven by the VFAs produced from in situ gas fermentation. Microbial community analysis showed an increased abundance of Bacteroidota group from 4.0 % to 18.5 %, which is capable of performing syngas fermentation, and the presence of heterotrophic denitrifiers (e.g., Thauera and Brachymonas), which are known to perform bromate reduction. Together these results for the first time demonstrated the feasibility of using H2/CO2 mixture for bromate removal coupled with in situ VFAs production. The findings can facilitate the development of cost-effective strategies for groundwater and drinking water remediation.

An integrated system combining Tetrasphaera-dominated enhanced biological phosphorus removal with sulfur autotrophic denitrification to enhance biological nutrients removal.

This study developed a two-stage process, including Tetrasphaera-dominated enhanced biological phosphorus-removal (EBPR(T)) sequencing batch reactor (SBR), followed by sulfur autotrophic denitrification (SADN) SBR, to achieve advanced nutrients removal from low VFAs wastewater. The removal efficiencies of nitrogen and phosphorus (PO43--P) reached 99 % with effluent PO43--P and total inorganic nitrogen (TIN) below 0.5 mg/L and 1 mg/L in EBPR(T) and SADN SBR, respectively. Mechanism analysis indicated that as increasing drainage ratio and complex carbon sources, free amino acids, glycogen, and PHA served as the endogenous carbon sources of Tetrasphaera to store energy. SADN contributed to approximately 80 % of nitrogen removal. DNA and cDNA results indicated Tetrasphaera was shifted from clade 2 to clade 1 after increasing the drainage ratio and the complexity of the carbon source, and Tetrasphaera (50.95 %) and Ca. Accumulibacter (9.12 %) were the most important functional microorganisms synergized to remove phosphorus at the transcriptional level in EBPR(T). Thiobacillus (45.97 %) and Sulfuritalea (9.24 %) were the dominant sulfur autotrophic denitrifiers at gene and transcriptional level in SADN. The results suggested that the EBPR(T) - SADN SBRs have great nutrient removal performance in treating low VFAs wastewater without additional carbon sources.

Granular activated carbon enhances volatile fatty acid production in the anaerobic fermentation of garden wastes.

Garden waste, one type of lignocellulosic biomass, holds significant potential for the production of volatile fatty acids (VFAs) through anaerobic fermentation. However, the hydrolysis efficiency of garden waste is limited by the inherent recalcitrance, which further influences VFA production. Granular activated carbon (GAC) could promote hydrolysis and acidogenesis efficiency during anaerobic fermentation. This study developed a strategy to use GAC to enhance the anaerobic fermentation of garden waste without any complex pretreatments and extra enzymes. The results showed that GAC addition could improve VFA production, especially acetate, and reach the maximum total VFA yield of 191.55 mg/g VSadded, which increased by 27.35% compared to the control group. The highest VFA/sCOD value of 70.01% was attained in the GAC-amended group, whereas the control group only reached 49.35%, indicating a better hydrolysis and acidogenesis capacity attributed to the addition of GAC. Microbial community results revealed that GAC addition promoted the enrichment of Caproiciproducens and Clostridium, which are crucial for anaerobic VFA production. In addition, only the GAC-amended group showed the presence of Sphaerochaeta and Oscillibacter genera, which are associated with electron transfer processes. Metagenomics analysis indicated that GAC addition improved the abundance of glycoside hydrolases (GHs) and key functional enzymes related to hydrolysis and acidogenesis. Furthermore, the assessment of major genera influencing functional genes in both groups indicated that Sphaerochaeta, Clostridium, and Caproicibacter were the primary contributors to upregulated genes. These findings underscored the significance of employing GAC to enhance the anaerobic fermentation of garden waste, offering a promising approach for sustainable biomass conversion and VFA production.

Astragalus additive in feed improved serum immune function, rumen fermentation and the microbiota structure of early-weaned lambs.

AIM: The purpose of this study was to determine the mechanism of Astragalus activity on the immune function, rumen microbiota structure, and rumen fermentation of early-weaned lambs. METHODS AND RESULTS: Thirty healthy early-weaned lambs with similar body weights (17.42 ± 2.02 kg) were selected for the feeding experiment. The control group (KB) was fed a basal diet, and the Astragalus group (HQ) was fed 0.3% Astragalus additive on the basis of a basic diet. The formal trial period was 60 days. The results showed that the concentrations of blood immunoglobulin A (IgA) and immunoglobulin M (IgM) in the HQ group were significantly higher than those in the KB group (P < 0.05). Compared with the KB group, the concentrations of acetic acid, butyric acid, and total volatile fatty acids (VFAs) in the HQ group were higher (P < 0.01). The expression levels of the rumen epithelial-related genes MCT1, MCT4, NHE2, and ZO1 in the Astragalus group were significantly higher than those in the KB group (P < 0.05). 16S rRNA analysis showed that at the phylum level, Bacteroidetes in the HQ group significantly increased (P < 0.01); at the genus level, Prevotella (P < 0.01) and Succiniclasticum (P < 0.01) in the HQ group were found at significantly higher abundances than those in the KB group, and the results of microbiota gene and function prediction showed that "energy metabolism," "glycan biosynthesis and metabolic" pathways were significantly enriched in the HQ group (P < 0.05). CONCLUSION: As a feed additive, Astragalus can improve the immunity of early-weaned lambs, the structure of the rumen microbiota of lambs, and the fermentation capacity of the rumen.

Evaluation of volatile fatty acids and ammonia recovery approach from landfill leachate using pilot-scale mechanical vapor recompression.

Treatment of landfill leachate is still a current problem due to the high treatment costs in addition to the difficulty of meeting the discharge criteria. However, there is a more important issue that should be underlined; it is also valuable compounds that leachate contains. Conventional methods used for treatment of leachate such as membrane filtration, advanced oxidation processes, biological processes and their combinations have largely focused on treatment. However, the recovery of ammonia and volatile organic acids (VFA) in leachate is a promising approach both to overcome high treatment costs and to sustainably manage leachate. In this study, leachate treatment potential was investigated by mechanical vapor recompression (MVR) process, which offers an operational opportunity to recover high value-added products from leachate while providing an effective treatment for wastewater. Optimum operating conditions for the pilot-scale MVR process have been determined by laboratory-scale studies. VFAs were recovered as organic acid salts from the pilot-scale MVR distillate, while ammonia recovery was accomplished as ammonium sulfate from a highly contaminated concentrate stream. VFA and ammonia recovery rates were 89% and 99%, respectively. The treatment cost of leachate with MVR process was calculated according to the data obtained in pilot scale MVR studies considering the operating cost, chemical cost and economical contribution of value-added products. The results showed that the integrated MVR-crystallization process, all treatment costs are covered, with a net gain of 3.8 USD/m3. Consequently, MVR integrated crystallization process offers an economical and sustainable solution for the treatment of leachate by recovering valuable products.

Thermo-alkaline pretreatment of excess sludge: Effects of temperature on volatile fatty acids accumulation and microbial community.

In order to explore the role of thermal-alkaline pretreatment temperatures (TAPT) in sludge fermentation and the microbial characteristics, five groups (100, 120, 140, 160 °C and control group) were set up and the results showed that the increasing TAPT promoted the dissolution of soluble chemical oxygen demand (SCOD) and VFAs, but had slight influence on the release of NH4+-N and PO43--P. What's more, when it was 120 °C, the SCOD dissolution was comparable to that at 160 °C. Overall, 120 °C was the optimal condition, corresponding to the fact that the maximum release of SCOD was 8788.74 mg/L (2.63 times of the control group), the maximum dissolution of VFAs was 4596 mg/L (about 1.28 times of the control group). The trend of C/N was not significant. High-throughput sequencing showed that Firmicutes and Actinobacteriota were enriched with the temperature increasing, while Proteobacteria and Chloroflexi did not change significantly. Firmicutes was in a stable dominant position. Temperature conditions brought about significant changes in microbial interspecific interaction. Carbohydrate and amino acids had the highest metabolic abundance, especially at 120 °C group. The change rule of amino acid metabolism was similar to that of lipid metabolism, and the abundance of energy metabolism gradually increased with temperature. The protein metabolism was greatly affected by temperature. This study revealed the effect of microbial mechanism of TAPT on the sludge acid production efficiency.

Emerging Strategies for Enhancing Propionate Conversion in Anaerobic Digestion: A Review.

Anaerobic digestion (AD) is a triple-benefit biotechnology for organic waste treatment, renewable production, and carbon emission reduction. In the process of anaerobic digestion, pH, temperature, organic load, ammonia nitrogen, VFAs, and other factors affect fermentation efficiency and stability. The balance between the generation and consumption of volatile fatty acids (VFAs) in the anaerobic digestion process is the key to stable AD operation. However, the accumulation of VFAs frequently occurs, especially propionate, because its oxidation has the highest Gibbs free energy when compared to other VFAs. In order to solve this problem, some strategies, including buffering addition, suspension of feeding, decreased organic loading rate, and so on, have been proposed. Emerging methods, such as bioaugmentation, supplementary trace elements, the addition of electronic receptors, conductive materials, and the degasification of dissolved hydrogen, have been recently researched, presenting promising results. But the efficacy of these methods still requires further studies and tests regarding full-scale application. The main objective of this paper is to provide a comprehensive review of the mechanisms of propionate generation, the metabolic pathways and the influencing factors during the AD process, and the recent literature regarding the experimental research related to the efficacy of various strategies for enhancing propionate biodegradation. In addition, the issues that must be addressed in the future and the focus of future research are identified, and the potential directions for future development are predicted.

Investigating the synergic role of asynchronous dosed protease and lysozyme for facilitating excess sludge solubilization and acidogenic fermentation.

Improving the anaerobic fermentation (AF) efficiency of excess sludge (ES) is essential for attaining biosolid minimization, stabilization, resource recovery, and carbon-emission reduction. Along these lines, here, the synergistic mechanism of protease and lysozyme for enhancing hydrolysis and AF efficiency with better recovery of volatile fatty acids (VFAs) was thoroughly investigated. Single lysozyme was capable of reducing the zeta potential and fractal dimension when dosed into the ES-AF system, which was beneficial for increasing the contact probability between proteases and extracellular proteins. Moreover, the weight-averaged molecular weight of the loosely-bound extracellular polymeric substance (LB-EPS) reduced from 1867 to 1490 in the protease-AF group, which facilitated the penetration of EPS by the lysozyme. The soluble DNA and extracellular DNA (eDNA) of the enzyme cocktail pretreated group increased by 23.24 % and 77.09 %, and the cell viability decreased after 6-hour hydrolysis, demonstrating a better hydrolysis efficiency. Remarkably, the asynchronous dosed enzyme cocktail pretreatment was proven a better strategy to enhance both the solubilization and hydrolysis processes since the synergistic effect of these two enzymes can exclude the mutual interference. As a result, the VFAs were increased by 1.26 times higher than the blank group. The underlying mechanism of an environmental-friendly and effective strategy was examined to promote ES hydrolysis and acidogenic fermentation, which was beneficial for the recovery of VFAs and carbon-emission reduction.

Zinc pyrithione induced volatile fatty acids promotion derived from sludge anaerobic digestion: Interrelating the affected steps with microbial metabolic regulation and adaptive responses.

The massive use of zinc pyrithione (ZPT, as broad-spectrum bactericides) resulted in its high levels in waste activated sludge (WAS) and affected subsequent WAS treatment. This work revealed the effects of ZPT on the volatile fatty acids (VFAs) during WAS anaerobic digestion, in which VFAs yield was enhanced by approximately 6-9 folds (from 353 mg COD/L in control to 2526-3318 mg COD/L with low level of ZPT (20-50 mg/g TSS)). The ZPT occurred in WAS enabled the acceleration of solubilization, hydrolysis and acidification processes while inhibited the methanogenesis. Also, the low ZPT contributed to the enrichment of functional hydrolytic-acidifying microorganisms (e.g., Ottowia and Acinetobacter) but caused the reduction of methanogens (e.g., Methanomassiliicoccus and Methanothrix). Meta-transcriptomic analysis demonstrated that the critical genes relevant to extracellular hydrolysis (i.e. CLPP and ZapA), membrane transport (i.e. gltI, and gltL), substrates metabolisms (i.e. fadj, and acd), and VFAs biosynthesis (i.e. porB and porD) were all upregulated by 25.1-701.3% with low level of ZPT. Specifically, the ZPT stimulus on amino acids metabolism for VFAs transformation was prominent over carbohydrates. Moreover, the functional species enabled to regulate the genes in QS and TCS systems to maintain favorable cell chemotaxis to adapt the ZPT stress. The cationic antimicrobial peptide resistance pathway was upregulated to blunt ZPT with the secretion of more lipopolysaccharide and activate proton pumps to maintain ions homeostasis to antagonize the ZPT toxicity for high microbial activities, the abundance of related genes was up-regulated by 60.5 to 524.5%. This work enlightened environmental behaviors of emerging pollutants on WAS anaerobic digestion process with interrelations of microbial metabolic regulation and adaptive responses.

Impact of Molasses on Ruminal Volatile Fatty Acid Production and Microbiota Composition In Vitro.

The aim of this study was to assess if molasses could modify VFA production and the rumen microbial community in vitro. Three beet (treatment Beet) and three cane (treatment Cane) molasses preparations were randomly selected from a variety of samples collected worldwide and incubated in vitro with rumen fluid along with a control sample (treatment CTR, in which no molasses was used). Flasks for VFA analysis were sampled at 0, 1, 2, 3, 4, 6, 8, and 24 h of each incubation. For microbiota analysis, samples from each fermentation flask after 12 and 24 h were subjected to microbial DNA extraction and V3-V4 16S rRNA gene sequencing on an Illumina MiSeq platform. Total net VFA production was higher in the beet and cane preparations than in the control (CTR) group at 24 h (33 mmol/L, 34 mmol/L, and 24.8 mmol/L, respectively), and the composition of VFAs was affected by the inclusion of molasses: acetic acid increased in the CTR group (73.5 mol%), while propionic acid increased in the beet and cane molasses (19.6 mol% and 18.6 mol%, respectively), and butyric acid increased, especially in the cane group (23.2 mol%). Molasses even influenced the composition of the rumen microbiota, and particularly the relative abundance of the most dominant family in the rumen, Prevotellaceae, which decreased compared to CTR (37.13%, 28.88%, and 49.6%, respectively). In contrast, Streptococcaceae (19.62% and 28.10% in molasses compared to 6.23% in CTR), Veillonellaceae (6.48% and 8.67% in molasses compared to 4.54% in CTR), and Fibrobacteraceae (0.90% and 0.88% in molasses compared to 0.62% in CTR) increased in the beet and cane groups compared to the CTR group. Another important finding is the lower proportion of Methanobacteriaceae following the addition of molasses compared to CTR (0.26%, 0.28%, and 0.43%, respectively). This study showed the impact of molasses in influencing VFA production and composition as a result of a modified rumen microbial composition.

Measurement of Volatile Fatty Acids in Silage through Odors with Nanomechanical Sensors.

The measurement of volatile fatty acids (VFAs) is of great importance in the fields of food and agriculture. There are various methods to measure VFAs, but most methods require specific equipment, making on-site measurements difficult. In this work, we demonstrate the measurements of VFAs in a model sample, silage, through its vapor using an array of nanomechanical sensors-Membrane-type Surface stress Sensors (MSS). Focusing on relatively slow desorption behaviors of VFAs predicted with the sorption kinetics of nanomechanical sensing and the dissociation nature of VFAs, the VFAs can be efficiently measured by using features extracted from the decay curves of the sensing response, resulting in sufficient discrimination of the silage samples. Since the present sensing system does not require expensive, bulky setup and pre-treatment of samples, it has a great potential for practical applications including on-site measurements.

Re-circulation of Fe/persulfate regulated sludge fermentation products for sewage treatment: Focus on pollutant removal efficiency, microbial community and metabolic activity.

Persulfate (PS)-based technologies have been demonstrated as efficient methods for enhancing the performance of waste activated sludge (WAS) anaerobic fermentation. Except for volatile fatty acids (VFAs), however, some exogenous substances would be also released during this process, which might affect its application as a carbon source for sewage treatment. To fill this knowledge gap, the feasibility of sludge fermentation liquid regulated by Fe/persulfate (PS) (PS-FL) as a carbon source for sewage treatment was investigated in this study. Results indicated that PS-FL exhibits distinct effects on the pollutants removal compared with commercial sodium acetate. It facilitates PO43--P removal but slightly inhibited COD removal & denitrification, and sludge settleability was also decreased. The mechanistic analysis demonstrated that PS-FL could stimulate the enrichment of phosphorus-accumulating bacteria (i.e. Candidatus Accumulibacter) and the enhancement of their metabolic activities (i.e. PKK), thereby enhancing the biological PO43--P removal. Moreover, Fe ions in PS-FL could combine with PO43--P to form a precipitate and thus further contributed to PO43--P removal. Conversely, the sulfate reduction process induced by SO42- in PS-FL inhibits denitrification by reducing the abundance of denitrifying bacteria (i.e. Dechloromonas) and metabolic activities (i.e. narG). Additionally, PS-FL also decreased the abundance of flocculation bacteria (i.e. Flavobacterium) and down-regulated the expression of functional genes responsible for COD removal, by which it exhibited certain negative effects on COD removal and sludge settleability. Overall, this work demonstrated that PS-FL can re-circulation as a carbon source for sewage treatment, which provides a new approach to recovering valuable carbon sources from WAS.