CuO nanoparticles' effect on the photosynthetic performance in seed tissues of Inga laurina (Fabaceae).

Copper oxide nanoparticles (CuONPs) have been produced on a large scale because they can be applied across various fields, especially in nano-enabled healthcare and agricultural products. However, the increasing use of CuONPs leads to their release and accumulation into the environment. The CuONPs uptaken by seeds and their implications on germination behavior have been reported, but little is known or understood about their impact on photosynthesis in seed tissues. To fill knowledge gaps, this study evaluated the effects of CuONP concentrations (0-300 mg L-1) on the photosynthetic activity of Inga laurina seeds. The microscopy data showed that CuONPs had an average size distribution of 57.5 ± 0.7 nm. Copper ion release and production of reactive oxygen species (ROS) by CuONPs were also evaluated by dialysis and spectroscopy experiments, respectively. CuONPs were not able to intrinsically generate ROS and released a low content of Cu2⁺ ions (4.5%, w/w). Time evolution of chlorophyll fluorescence imaging and laser-induced fluorescence spectroscopy were used to monitor the seeds subjected to nanoparticles during 168 h. The data demonstrate that CuONPs affected the steady-state maximum chlorophyll fluorescence ( F m ' ), the photochemical efficiency of photosystem II ( F v / F m ), and non-photochemical quenching ( NPQ ) of Inga laurina seeds over time. Besides, the NPQ significantly increased at the seed development stage, near the root protrusion stage, probably due to energy dissipation at this germination step. Additionally, the results indicated that CuONPs can change the oscillatory rhythms of energy dissipation of the seeds, disturbing the circadian clock. In conclusion, the results indicate that CuONPs can affect the photosynthetic behavior of I. laurina seeds. These findings open opportunities for using chlorophyll fluorescence as a non-destructive tool to evaluate nanoparticle impact on photosynthetic activity in seed tissues.

The light intensity in the cultivation environment and the impact of glyphosate on plants of the Urochloa genus.

The variation in light within the environment triggers morphophysiological changes in plants and can lead to distinct responses in sun-exposed or shaded plants to glyphosate. The response of Urochloa genotypes subjected to desiccation with 2160, 1622.4, 1080, 524.4, 273.6, and 0.0 g ha-1 of glyphosate was evaluated in full sun and shade conditions. Cayana grass, mulato II grass, and sabiá grass - hybrids recently launched on the market, in addition to palisade grass and congo grass were evaluated. Under full sun, we achieved control of congo grass using 1080 g ha-1 of glyphosate, while the other grasses required 2160 g ha-1. In the low-light environment, sabiá grass was effectively controlled with 524.4 g ha-1 of glyphosate, but the other grasses needed 273.6 g ha-1. In shading, compared to full sun, the savings with glyphosate were 75 and 76% for the control of congo grass and sabiá grass, respectively, and 87% for palisade grass, mulato II grass and cayana grass. Increasing glyphosate doses leads to a decline in the quantum efficiency of photosystem II and in the electron transport rate, especially in the shade. Urochloa genotypes are more sensitive to glyphosate in the shade, which must be considered when determining the herbicide dose.

Chlorophyll fluorescence in sentinel plants for the surveillance of chemical risk.

This research aimed to develop natural plant systems to serve as biological sentinels for the detection of organophosphate pesticides in the environment. The working hypothesis was that the presence of the pesticide in the environment caused changes in the content of pigments and in the photosynthetic functioning of the plant, which could be evaluated non-destructively through the analysis of reflected light and emitted fluorescence. The objective of the research was to furnish in vivo indicators derived from spectroscopic parameters, serving as early alert signals for the presence of organophosphates in the environment. In this context, the effects of two pesticides, Chlorpyrifos and Dimethoate, on the spectroscopic properties of aquatic plants (Vallisneria nana and Spathyfillum wallisii) were studied. Chlorophyll-a variable fluorescence allowed monitoring both pesticides' presence before any damage was observed at the naked eye, with the analysis of the fast transient (OJIP curve) proving more responsive than Kautsky kinetics, steady-state fluorescence, or reflectance measurements. Pesticides produced a decrease in the maximum quantum yield of PSII photochemistry, in the proportion of PSII photochemical deexcitation relative to PSII non photochemical decay and in the probability that trapped excitons moved electrons into the photosynthetic transport chain beyond QA-. Additionally, an increase in the proportion of absorbed energy being dissipated as heat rather than being utilized in the photosynthetic process, was notorious. The pesticides induced a higher deactivation of chlorophyll excited states by photophysical pathways (including fluorescence) with a decrease in the quantum yields of photosystem II and heat dissipation by non-photochemical quenching. The investigated aquatic plants served as sentinels for the presence of pesticides in the environment, with the alert signal starting within the first milliseconds of electronic transport in the photosynthetic chain. Organophosphates damage animals' central nervous systems similarly to certain compounds found in chemical weapons, thus raising the possibility that sentinel plants could potentially signal the presence of such weapons.

Associative Bacteria and Arbuscular Mycorrhizal Fungus Increase Drought Tolerance in Maize (Zea mays L.) through Morphoanatomical, Physiological, and Biochemical Changes.

Water deficiency has been recognized as a major abiotic stress that causes losses in maize crops around the world. The maize crop is very important due to the range of products that are derived from this plant. A potential way to reduce the damages caused by water deficiency in maize crops is through the association with plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF). To define the mechanisms developed by associative PGPB and AMF in maize that are involved in protection against moderate drought (MD), this study evaluated the biometrical, anatomical, biochemical, and physiological parameters of maize grown under MD and inoculated with different PGPB (Azospirillum brasilense strain Ab-V5 and Bacillus sp. strain ZK) and with AMF. The relative water content did not change in the treatments. The association with ZK increased the shoot:total ratio, total dry weight, maximum quantum yield of photosystem II, vascular cylinder thickness, and vascular cylinder area. The Ab-V5 inoculation led to an increment in root dry weight, the area of metaxylem vessel elements, and nitrate reductase activity. The AMF association did not lead to changes in the measured parameters. The results indicate that the association with PGPB is a relevant alternative to contribute to reducing losses in maize crops under drought. However, AMF is not indicated for this crop under drought.

Desiccation Tolerance of Epiphytic Macrolichens in an Evergreen Temperate Rain Forest (Alerce Costero National Park, Chile).

The Valdivian region has a temperate rainy climate with differences in rainfall throughout the year. This heterogeneity results in periods of summer drought that expose the poikilohydric epiphytes to desiccation. With this research, we aim to answer different research questions related to phorophyte preference, response to desiccation, and response to radiation. How does the diversity of macrolichens vary at a local and microclimate scale in three tree species within an evergreen forest? What is the tolerance limit of macrolichens against prolonged desiccation, according to evaluation of the maximum efficiency of PSII (Fv/Fm) and pigment concentration? What is the tolerance limit against a potential increase in radiation? We found that macrolichen communities are determined by tree species, which regulate the suitability of the substrate by modifying the temperature and humidity conditions. In addition, our results show a rapid photosynthetic alteration in temporal exposure to desiccation, measured through Fv/Fm and pigment concentration. Our results showed that the most sensitive lichens to radiation and desiccation are not coincident. We confirm the low tolerance of macrolichen species to high radiation, reflected in the saturation profile obtained for the set studied. The lichen community in the evergreen forest showed high complexity and vulnerability, pointing to the importance of more research.

Decreased Photosynthetic Efficiency in Nicotiana tabacum L. under Transient Heat Stress.

Heat stress is an abiotic factor that affects the photosynthetic parameters of plants. In this study, we examined the photosynthetic mechanisms underlying the rapid response of tobacco plants to heat stress in a controlled environment. To evaluate transient heat stress conditions, changes in photochemical, carboxylative, and fluorescence efficiencies were measured using an infrared gas analyser (IRGA Licor 6800) coupled with chlorophyll a fluorescence measurements. Our findings indicated that significant disruptions in the photosynthetic machinery occurred at 45 °C for 6 h following transient heat treatment, as explained by 76.2% in the principal component analysis. The photosynthetic mechanism analysis revealed that the dark respiration rate (Rd and Rd*CO2) increased, indicating a reduced potential for carbon fixation during plant growth and development. When the light compensation point (LCP) increased as the light saturation point (LSP) decreased, this indicated potential damage to the photosystem membrane of the thylakoids. Other photosynthetic parameters, such as AMAX, VCMAX, JMAX, and ΦCO2, also decreased, compromising both photochemical and carboxylative efficiencies in the Calvin-Benson cycle. The energy dissipation mechanism, as indicated by the NPQ, qN, and thermal values, suggested that a photoprotective strategy may have been employed. However, the observed transitory damage was a result of disruption of the electron transport rate (ETR) between the PSII and PSI photosystems, which was initially caused by high temperatures. Our study highlights the impact of rapid temperature changes on plant physiology and the potential acclimatisation mechanisms under rapid heat stress. Future research should focus on exploring the adaptive mechanisms involved in distinguishing mutants to improve crop resilience against environmental stressors.

Influence of Increase in Phosphorus Supply on Agronomic, Phenological, and Physiological Performance of Two Common Bean Breeding Lines Grown in Acidic Soil under High Temperature Stress Conditions.

Many common bean (Phaseolus vulgaris L.) plants cultivated in areas of the world with acidic soils exhibit difficulties adapting to low phosphorus (P) availability, along with aluminum (Al) toxicity, causing yield loss. The objective of this study was to evaluate the influence of an increase in P supply level on the agronomic, phenological, and physiological performance of two common bean breeding lines grown in acidic soil, with low fertility and under high temperature conditions, in a screenhouse. A randomized complete block (RCB) design was used under a factorial arrangement (five levels of P × 2 genotypes) for a total of 10 treatments with four replications. The factors considered in the experiment were: (i) five P supply levels (kg ha-1): four levels of P0, P15, P30, and P45 through the application of rock phosphate (RP), and one P level supplied through the application of organic matter (PSOM) corresponding to 25 kg P ha-1 (P25); and (ii) two advanced bean lines (BFS 10 and SEF10). Both bean lines were grown under the combined stress conditions of high temperatures (day and night maximum temperatures of 42.5 °C/31.1 °C, respectively) and acidic soil. By increasing the supply of P, a significant effect was found, indicating an increase in the growth and development of different vegetative organs, as well as physiological efficiency in photosynthesis and photosynthate remobilization, which resulted in higher grain yield in both bean lines evaluated (BFS 10 and SEF10). The adaptive responses of the two bean lines were found to be related to phenological adjustments (days to flowering and physiological maturity; stomatal development), as well as to heat dissipation strategies in the form of heat (NPQ) or unregulated energy (qN) that contributed to greater agronomic performance. We found that, to some extent, increased P supply alleviated the negative effects of high temperature on the growth and development of the reproductive organs of bean lines. Both bean lines (BFS 10 and SEF 10) showed adaptive attributes suited to the combined stress conditions of high temperature and acidic soil, and these two lines can serve as useful parents in a bean breeding program to develop multiple stress tolerant cultivars.

Two Congeneric Shrubs from the Atacama Desert Show Different Physiological Strategies That Improve Water Use Efficiency under a Simulated Heat Wave.

Desert shrubs are keystone species for plant diversity and ecosystem function. Atriplex clivicola and Atriplex deserticola (Amaranthaceae) are native shrubs from the Atacama Desert that show contrasting altitudinal distribution (A. clivicola: 0-700 m.a.s.l.; A. deserticola: 1500-3000 m.a.s.l.). Both species possess a C4 photosynthetic pathway and Kranz anatomy, traits adaptive to high temperatures. Historical records and projections for the near future show trends in increasing air temperature and frequency of heat wave events in these species' habitats. Besides sharing a C4 pathway, it is not clear how their leaf-level physiological traits associated with photosynthesis and water relations respond to heat stress. We studied their physiological traits (gas exchange, chlorophyll fluorescence, water status) before and after a simulated heat wave (HW). Both species enhanced their intrinsic water use efficiency after HW but via different mechanisms. A. clivicola, which has a higher LMA than A. deserticola, enhances water saving by closing stomata and maintaining RWC (%) and leaf Ψmd potential at similar values to those measured before HW. After HW, A. deserticola showed an increase of Amax without concurrent changes in gs and a significant reduction of RWC and Ψmd. A. deserticola showed higher values of Chla fluorescence after HW. Thus, under heat stress, A. clivicola maximizes water saving, whilst A. deserticola enhances its photosynthetic performance. These contrasting (eco)physiological strategies are consistent with the adaptation of each species to their local environmental conditions at different altitudes.

Unpredicted, rapid and unintended structural and functional changes occurred during early domestication of Silphium integrifolium, a perennial oilseed.

MAIN CONCLUSION: Selection for increased yield changed structure, physiology and overall resource-use strategy from conservative towards acquisitive leaves. Alternative criteria can be considered, to increase yield with less potentially negative traits. We compared the morphology, anatomy and physiology of wild and semi-domesticated (SD) accessions of Silphium integrifolium (Asteraceae), in multi-year experiments. We hypothesized that several cycles of selection for seed-yield would result in acquisitive leaves, including changes predicted by the leaf economic spectrum. Early-selection indirectly resulted in leaf structural and functional changes. Leaf anatomy changed, increasing mesophyll conductance and the size of xylem vessels and mesophyll cells increased. Leaves of SD plants were larger, heavier, with lower stomatal conductance, lower internal CO2 concentration, and lower resin concentration than those of wild types. Despite increased water use efficiency, SD plants transpired 25% more because their increase in leaf area. Unintended and undesired changes in functional plant traits could quickly become fixed during domestication, shortening the lifespan and increasing resource consumption of the crop as well as having consequences in the provision and regulation of ecosystem services.

Exploring the Physiological Multiplicity of Native Microalgae from the Ecuadorian Highland, Italian Lowland and Indoor Locations in Response to UV-B.

The differential effects of UV-B on the inhibition or activation of protective mechanisms to maintain cells photosynthetically active were investigated in native microalgae. Four strains were used, including two Chlorella sorokiniana strains, F4 and LG1, isolated from a Mediterranean inland swamp and a recycled cigarette butt's substrate, respectively, and two isolates from an Ecuadorian highland lake related to Pectinodesmus pectinatus (PEC) and Ettlia pseudoalveolaris (ETI). Monocultures were exposed to acute UV-B (1.7 W m-2) over 18 h under controlled conditions. UV-B-untreated microalgae were used as the control. Comparative physiological responses, including photosynthetic pigments, non-enzymatic antioxidants, and chlorophyll a fluorescence, were evaluated at specific time points. Results showed that UV-B significantly compromised all the physiological parameters in F4, thereby resulting in the most UV-B-sensitive strain. Contrarily, UV-B exposure did not lead to changes in the PEC physiological traits, resulting in the best UV-B-resistant strain. This could be attributed to the acclimation to high light habitat, where maintaining a constitutive phenotype (at the photosynthetic level) is strategically advantageous. Differently, LG1 and ETI at 12 h of UV-B exposure showed different UV-B responses, which is probably related to acclimation, where in LG1, the pigments were recovered, and the antioxidants were still functioning, while in ETI, the accumulation of pigments and antioxidants was increased to avoid further photodamage. Consequently, the prolonged exposure in LG1 and ETI resulted in species-specific metabolic regulation (e.g., non-enzymatic antioxidants) in order to constrain full photoinhibition under acute UV-B.

Ionic homeostasis and redox metabolism upregulated by 24-epibrassinolide are crucial for mitigating nickel excess in soybean plants, enhancing photosystem II efficiency and biomass.

Nickel (Ni) excess often generates oxidative stress in chloroplasts, causing redox imbalance, membrane damage and negative impacts on biomass. 24-Epibrassinolide (EBR) is a plant growth regulator of great interest to the scientific community because it is a natural molecule extracted from plants, is biodegradable and environmentally friendly. This study aimed to determine whether EBR can improve ionic homeostasis, antioxidant enzymes, PSII efficiency and biomass by evaluating nutritional, physiological, biochemical and morphological responses of soybean plants subjected to Ni excess. The experiment used four randomized treatments, with two Ni concentrations (0 and 200 µm Ni, described as -Ni2+ and +Ni2+ , respectively) and two concentrations of EBR (0 and 100 nm EBR, described as -EBR and +EBR, respectively). In general, Ni had deleterious effects on chlorophyll fluorescence and gas exchange. In contrast, EBR enhanced the effective quantum yield of PSII photochemistry (15%) and electron transport rate (19%) due to upregulation of SOD, CAT, APX and POX. Exogenous EBR application promoted significant increases in biomass, and these results were explained by improved nutrient content and ionic homeostasis, as demonstrated by increased Ca2+ /Ni2+ , Mg2+ /Ni+2 and Mn2+ /Ni2+ ratios.

Determining the ecophysiological limits of a narrow niche tropical conifer tree (Podocarpus trinitensis).

Many tropical species live close to their thermal limits within a narrow niche. Here, we investigate the ecophysiological limits of the tropical tree Podocarpus trinitensis, which is endemic to Trinidad and Tobago where most populations exist as isolated stands on hilltops. Five wild stands from a range of elevations were compared in the field with measurements of leaf temperature, canopy cover, stomatal conductance (gs), chlorophyll content and several chlorophyll fluorescence parameters. A parallel greenhouse experiment was used to acclimate seedlings to 'CONTROL' and 'HEAT' treatments (with mid-day air temperatures of 34.5 and 37 °C respectively), after which the above parameters were measured along with photosynthetic light and temperature response curves, leaf morphology and in vitro Fv/Fm thermostability. There was a positive association between improved physiological performance and elevation. In the high elevation sites, leaf temperatures were significantly lower while most of the physiological parameters were higher (gs, chlorophyll content, ɸ PSII, ETRmax and Isat90). In the greenhouse, HEAT and CONTROL plants were similar for most parameters, except leaf temperature (which was coupled with air temperature) and leaf mass per unit area (which was higher in HEAT plants). Temperature response curves showed an optimum temperature for photosynthesis of 30 ± 0.5 °C (TOpt) and in vitro Fv/Fm indicated a critical temperature of 47.4 ± 0.38 °C for HEAT and 48.2 ± 0.24 °C for CONTROL (T50), with no indication of heat acclimation. Podocarpus trinitensis was found to be shade tolerant. In the field, seedlings established under a close canopy (>95% canopy cover) and had a low light saturation point (LCP). In the greenhouse, where more light was available, seedlings retained a low light compensation point, light saturation point (LSP) and maximum photosynthetic rate (Amax). The results suggest that P. trinitensis is moderately heat tolerant with the higher elevation sites being more habitable, but stands are also able to survive near sea level under a closed canopy. The narrow niche, along with the 30 ± 0.5 °C optimum temperature for photosynthesis and the lack of thermal plasticity in critical temperature, suggests that P. trinitensis has little room to acclimate to temperatures higher than those currently experienced.

Impact of Web Blight on Photosynthetic Performance of an Elite Common Bean Line in the Western Amazon Region of Colombia.

Disease stress caused by plant pathogens impacts the functioning of the photosynthetic apparatus, and the symptoms caused by the degree of severity of the disease can generally be observed in different plant parts. The accurate assessment of plant symptoms can be used as a proxy indicator for managing disease incidence, estimating yield loss, and developing genotypes with disease resistance. The objective of this work was to determine the response of the photosynthetic apparatus to the increased disease severity caused by web blight Thanatephorus cucumeris (Frank) Donk on the common bean (Phaseolus vulgaris L.) leaves under acidic soil and the humid tropical conditions of the Colombian Amazon. Differences in chlorophyll fluorescence parameters, including Fv/Fm, Y(II), Y(NPQ), Y(NO), ETR, qP, and qN in leaves with different levels of severity of web blight in an elite line (BFS 10) of common bean were evaluated under field conditions. A significant effect of web blight on the photosynthetic apparatus was found. A reduction of up to 50% of energy use dedicated to the photosynthetic machinery was observed, even at the severity scale score of 2 (5% surface incidence). The results from this study indicate that the use of fluorescence imaging not only allows for the quantifying of the impact of web blight on photosynthetic performance, but also for detecting the incidence of disease earlier, before severe symptoms occur on the leaves.

Antarctic Lichens under Long-Term Passive Warming: Species-Specific Photochemical Responses to Desiccation and Heat Shock Treatments.

Climate warming in the Antarctic tundra will affect locally dominant cryptogams. Being adapted to low temperatures and freezing, little is known about the response of the polar lichens' primary photochemistry to warming and desiccation. Since 2008, we have monitored the ecophysiological responses of lichens to the future warming scenario during a long-term warming experiment through open top chambers (OTCs) on Fildes Peninsula. We studied the primary photochemical response (potential Fv/Fm and effective efficiency of photosystem II YPSII) of different lichen taxa and morphotypes under desiccation kinetics and heat shock experiments. As lichens grow slowly, to observe changes during warming we methodologically focused on carbon and nitrogen content as well as on the stable isotope ratios. Endemic Himantormia lugubris showed the strongest effect of long-term warming on primary photochemistry, where PSII activity occurred at a lower %RWC inside the OTCs, in addition to higher Fv/Fm values at 30 °C in the heat shock kinetic treatment. In contrast, Usnea aurantiaco-atra did not show any effect of long-term warming but was active at a thallus RWC lower than 10%. Both Cladonia species were most affected by water stress, with Cladonia aff. gracilis showing no significant differences in primary photochemical responses between the warming and the control but a high sensibility to water deficiency, where, at 60% thallus RWC, the photochemical parameters began to decrease. We detected species-specific responses not only to long-term warming, but also to desiccation. On the other hand, the carbon content did not vary significantly among the species or because of the passive warming treatment. Similarly, the nitrogen content showed non-significant variation; however, the C/N ratio was affected, with the strongest C/N decrease in Cladonia borealis. Our results suggest that Antarctic lichens can tolerate warming and high temperature better than desiccation and that climate change may affect these species if it is associated with a decrease in water availability.

Improved photosynthetic performance induced by Fe3O4 nanoparticles.

Interaction between 11 nm-sized magnetite nanoparticles and Cichorium intybus plants was studied in this work. In particular, the effect of these nanoparticles on the photosynthesis electron chain was carefully analysed. Magnetite nanoparticles were synthesised and physically characterised by Transmission electron microscopy (TEM), Scanning electron microscopy (SEM)), Energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), Magnetic hysteresis cycles and UV-visible spectroscopy. Suspensions of the obtained magnetite nanoparticles with different concentrations (10-1000 ppm) were sprayed over chicory leaves and their photosynthetic activity was evaluated using chlorophyll fluorescence techniques. The study was complemented with the determination of pigment concentration and spectral reflectance indices. The whole set of results was compared to those obtained for control (non-treated) plants. Magnetite nanoparticles caused an increment in the content of Chlorophyll a (up to 36%) and Chlorophyll b (up to 41%). The ratio Chlorophyll/ Carotenoids significantly increased (up to 29%) and the quotient Chlorophyll a/b remained relatively constant, except for a sharp increase (19%) at 100 ppm. The reflectance index that best manifested the improvement in chlorophyll content was the modified Normalised Difference Vegetation Index (mNDI), with a maximum increase of about 35%. Electronic transport fluxes were favoured and the photosynthetic parameters derived from Kautsky's kinetics were improved. An optimal concentration of nanoparticles (100 ppm) for the most beneficial effects on photosynthesis was identified. For this dose, the probability by which a trapped electron in PSII was transferred up to PSI acceptors (ΦRE0) was doubled and the parameter that quantifies the energy conservation of photons absorbed by PSII up to the reduction of PSI acceptors ([Formula: see text]), augmented five times. The fraction of absorbed energy used for photosynthesis increased to 86% and the energy lost as heat by the non-photochemical quenching mechanism was reduced to 31%. Beyond 100 ppm, photosynthetic parameters declined but remained above the values of the control.

A Reliable Method to Recognize Soybean Seed Maturation Stages Based on Autofluorescence-Spectral Imaging Combined With Machine Learning Algorithms.

In recent years, technological innovations have allowed significant advances in the diagnosis of seed quality. Seeds with superior physiological quality are those with the highest level of physiological maturity and the integration of rapid and precise methods to separate them contributes to better performance in the field. Autofluorescence-spectral imaging is an innovative technique based on fluorescence signals from fluorophores present in seed tissues, which have biological implications for seed quality. Thus, through this technique, it would be possible to classify seeds in different maturation stages. To test this, we produced plants of a commercial cultivar (MG/BR 46 "Conquista") and collected the seeds at five reproductive (R) stages: R7.1 (beginning of maturity), R7.2 (mass maturity), R7.3 (seed disconnected from the mother plant), R8 (harvest point), and R9 (final maturity). Autofluorescence signals were extracted from images captured at different excitation/emission combinations. In parallel, we investigated physical parameters, germination, vigor and the dynamics of pigments in seeds from different maturation stages. To verify the accuracy in predicting the seed maturation stages based on autofluorescence-spectral imaging, we created machine learning models based on three algorithms: (i) random forest, (ii) neural network, and (iii) support vector machine. Here, we reported the unprecedented use of the autofluorescence-spectral technique to classify the maturation stages of soybean seeds, especially using the excitation/emission combination of chlorophyll a (660/700 nm) and b (405/600 nm). Taken together, the machine learning algorithms showed high performance segmenting the different stages of seed maturation. In summary, our results demonstrated that the maturation stages of soybean seeds have their autofluorescence-spectral identity in the wavelengths of chlorophylls, which allows the use of this technique as a marker of seed maturity and superior physiological quality.

Combined Effect of Microplastics and Cd Alters the Enzymatic Activity of Soil and the Productivity of Strawberry Plants.

The synergistic effect between heavy metals and microplastics can affect soil properties as well as plant performance and yield. The objective of this study was to evaluate the combined effect of microplastics and cadmium on a soil-plant system. Specifically, we proposed to explore changes in soil microbiological activity, the growth and yield parameters of strawberry plants, and to evaluate the accumulation of these pollutants in the soil and root system. Plants were planted in clay pots under greenhouse conditions. The experiment was set up as a completely randomized design, with four treatments (Control; MPs; Cd; and Cd + MPs) and five replicates. The results showed that MPs and/or Cd affected plant growth, plant biomass, the number of fruits, root characteristics, dehydrogenase activity, acid phosphatase, and microbial biomass, and increased the accumulation of Cd in the roots and soil. The increased bioavailability of Cd, due to the presence of microplastics, could explain the observed negative effects on soil properties and the performance of strawberry plants.

Ecophysiology and water use efficiency of soybean cultivars under field conditions

A wide range of soybean cultivars is available on the market and understanding the physiological response and yield of these materials is fundamental to develop new management systems. Thus, the objective of the present study was to assess ecophysiological parameters and yield of soybean cultivars under field conditions. The experiment was carried out on a farm located in the municipality of Açailândia, Maranhão, Brazil. Three commercial cultivars were used (SC1, SC2 and SC3), and gas exchanges, SPAD index, Fv/Fm, photosynthesis index (PI), instantaneous water use efficiency (WUE) and intrinsic instantaneous of the use of water (iWUE) were assessed during the vegetative (V5) and reproductive (R5) stages. In addition, the biomass and production components were obtained. A randomized complete block design was used, with three cultivars and six replications. SC2 obtained the best mean for the photochemical variables. SC2 was more efficient at both development stages in WUE, but the maximum iWUE values were obtained in SC3. The SC2 cultivar obtained the best responses in the main variables analyzed, resulting in a higher yield.

Glyphosate hormesis attenuates water deficit stress in safflower (Carthamus tinctorius L.) by modulating physiological and biochemical mediators.

Changes in photosynthetic machinery can induce physiological and biochemical damage in plants. Low doses of glyphosate have been shown to exert a positive effect in mitigating the deleterious effects of water deficit in plants. Here, the physiological and biochemical mechanisms of safflower plants (Carthamus tinctorius L.) were studied under conditions of water deficit mediated by the attenuating effect of low-dose glyphosate. The plants were divided into two groups of water regimes in soil, without water deficit (-10 kPa) and with water deficit (-70 kPa), and were exposed to different concentrations of glyphosate (0, 1.8, 3.6, 7.2, 18, 36, 72, 180, 360, and 720 g a.e. ha-1). Evident protective responses at the physiological and biochemical levels were obtained after applying low doses of glyphosate to plants under water deficit, with a limiting dose for the occurrence of hormesis (LDS) = 72 g a.e. ha-1. The water deficit in plants resulted in hydrogen peroxide (H2O2) accumulation and consequently lipid peroxidation (LPO) associated with the accumulation of shikimic acid and glyphosate in plants, which triggered an increase in the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) that act by dismuting the levels of reactive oxygen species (ROS), maintaining, and/or increasing the maximum quantum efficiency of photosystem II (Fv/Fm), effective quantum yield of photosystem II (ΦPSII), electron transport rate (ETR), photochemical extinction coefficient (qP), and non-photochemical extinction coefficient (NPQ). APX appears to be the main enzyme involved in eliminating H2O2. Low doses of glyphosate act as water deficit ameliorators, allowing the plant to maintain/increase metabolism at physiological and biochemical levels by activating antioxidant enzymes in the dismutation of ROS in safflower plants.

Evaluation of the molecular and physiological response to dehydration of two accessions of the model plant Setaria viridis.

Water deficits are responsible for countless agricultural losses. Among the affected crops, C4 plants are of special interest due to their high water and nitrogen use efficiency. Two accessions of Setaria viridis (Ast-1 and A10.1) with contrasting responses to water deficit were used in the current work to better understand the mechanisms behind drought tolerance in C4 species. Our results showed that although the A10.1 accession exhibited a reduced size and lower Rfd values in comparison to Ast-1, it had overall higher Fv/Fm and lower NPQ values in well-watered conditions. The water deficit induction was performed with PEG-8000 at the grain-filling stage using dehydration cycles. Analysis of physiological measurements showed the A10.1 accession as being more tolerant to multiple water deficit exposures. In addition, PCA identified a clear difference in the pattern of drought response of the accessions. Four drought marker genes previously described in the literature were chosen to evaluate the response at the molecular level: SvP5CS2, SvDHN1, SvNAC6, and SvWRKY1. Besides confirming that Ast-1 is a more sensitive accession, the expression analysis revealed that SvNAC1 might better monitor drought stress, while SvWRKY1 was able to differentiate the two accessions. Distinct evolutionary histories of each accession may be behind their differences in response to water deficits.