Highly pathogenic avian influenza A(H5N1) virus of clade 2.3.4.4b isolated from a human case in Chile causes fatal disease and transmits between co-housed ferrets.

Clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) viruses have caused large outbreaks within avian populations on five continents, with concurrent spillover into a variety of mammalian species. Mutations associated with mammalian adaptation have been sporadically identified in avian isolates, and more frequently among mammalian isolates following infection. Reports of human infection with A(H5N1) viruses following contact with infected wildlife have been reported on multiple continents, highlighting the need for pandemic risk assessment of these viruses. In this study, the pathogenicity and transmissibility of A/Chile/25945/2023 HPAI A(H5N1) virus, a novel reassortant with four gene segments (PB1, PB2, NP, MP) from North American lineage, isolated from a severe human case in Chile, was evaluated in vitro and using the ferret model. This virus possessed a high capacity to cause fatal disease, characterized by high morbidity and extrapulmonary spread in virus-inoculated ferrets. The virus was capable of transmission to naïve contacts in a direct contact setting, with contact animals similarly exhibiting severe disease, but did not exhibit productive transmission in respiratory droplet or fomite transmission models. Our results indicate that the virus would need to acquire an airborne transmissible phenotype in mammals to potentially cause a pandemic. Nonetheless, this work warrants continuous monitoring of mammalian adaptations in avian viruses, especially in strains isolated from humans, to aid pandemic preparedness efforts.

Not cat-scratch disease: Bartonella henselae neuroretinitis associated with non-feline pet mammals.

To describe the ocurrence of Bartonella-associated neuroretinitis secondary to non-feline pet exposure, we retrospectively reviewed medical records and imaging from patients with a clinical and serologic diagnosis of Bartonella henselae (BH). Retinal imaging included color fundus photography, optical coherence tomography (OCT) and fluorescein angiography (FA). Four eyes of two patients with cat-scratch disease were included in this study, with a mean age of 35 years. The mean follow-up was 13 months, after presentation of infectious neuroretinitis. Both patients suffered from bilateral neuroretinitis after direct contact with family pets (ferret and guinea pig). All patients were treated with a long-term systemic antimicrobial therapy. Visual acuity in all improved to 20/30 or better at six months. In conclusion, humans may develop cat-scratch disease when they are exposed to Bartonella henselae (BH) in the saliva of infected cats or BH-containing flea feces reaching the systemic circulation through scratches or mucous membranes. As the cat flea (Ctenocephalides felis) may reside on non-feline mammals, Bartonella-associated neuroretinitis may result from contact with other furred family pets.

Long-term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Mustela Nigripes).

Systematic cryo-banking of semen and testicular tissues is critical to preserve the genetic value of recently deceased or neutered black-footed ferrets (BFFs). Specifically, recovering or producing mature sperm cells from vitrified-warmed issues offers additional options in assisted reproduction. This could, in turn, enhance the genetic management of this rare and endangered species over multiple generations. The objective of the study was to evaluate structural properties, DNA fragmentation, cell viability, and germ cell composition in vitrified testicular tissues from BFFs directly after warming or after warming plus a short in vitro culture period. Tissue biopsies from five adult BFFs were either kept fresh or vitrified with a standard protocol (using dimethylsulphoxide (DMSO) and glycerol) and warmed at 50 °C for 5 s. Some of the warmed samples were then cultured in vitro for 24 h. Fresh, warmed, and warmed/cultured tissues were analyzed using different indicators: histology of seminiferous tubules, intact Sertoli cells (vimentin labeling), DNA integrity, cell viability, germ cell composition (Oct4 and Boule labeling). Percentages of intact seminiferous tubules decreased after vitrification/warming and returned to the level of fresh samples after culture. While percentages of cells labeled with vimentin, with intact DNA integrity, or proportions of viable cells were affected by vitrification/warming, they all reached similar or better levels than the fresh tissue after culture. Proportions of cells labeled with Boule antibodies also improved during in vitro culture post-warming. We demonstrated for the first time that BFF testes subjected to vitrification, rapid warming, and short in vitro culture were viable and maintained the ability to resume germ cell progression. Cryopreserved testicular tissues could potentially contribute to new strategies to enhance BFF assisted reproduction as well as conservation efforts.

Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens.

The development of multivalent vaccines is an attractive methodology for the simultaneous prevention of several infectious diseases in vulnerable populations. Both canine distemper virus (CDV) and rabies virus (RABV) cause lethal disease in wild and domestic carnivores. While RABV vaccines are inactivated, the live-attenuated CDV vaccines retain residual virulence for highly susceptible wildlife species. In this study, we developed recombinant bivalent vaccine candidates based on recombinant vaccine strain rabies virus particles, which concurrently display the protective CDV and RABV glycoprotein antigens. The recombinant viruses replicated to near-wild-type titers, and the heterologous glycoproteins were efficiently expressed and incorporated in the viral particles. Immunization of ferrets with beta-propiolactone-inactivated recombinant virus particles elicited protective RABV antibody titers, and animals immunized with a combination of CDV attachment protein- and fusion protein-expressing recombinant viruses were protected from lethal CDV challenge. However, animals that were immunized with only a RABV expressing the attachment protein of CDV vaccine strain Onderstepoort succumbed to infection with a more recent wild-type strain, indicating that immune responses to the more conserved fusion protein contribute to protection against heterologous CDV strains.IMPORTANCE Rabies virus and canine distemper virus (CDV) cause high mortality rates and death in many carnivores. While rabies vaccines are inactivated and thus have an excellent safety profile and high stability, live-attenuated CDV vaccines can retain residual virulence in highly susceptible species. Here we generated recombinant inactivated rabies viruses that carry one of the CDV glycoproteins on their surface. Ferrets immunized twice with a mix of recombinant rabies viruses carrying the CDV fusion and attachment glycoproteins were protected from lethal CDV challenge, whereas all animals that received recombinant rabies viruses carrying only the CDV attachment protein according to the same immunization scheme died. Irrespective of the CDV antigens used, all animals developed protective titers against rabies virus, illustrating that a bivalent rabies virus-based vaccine against CDV induces protective immune responses against both pathogens.

Femoral fracture repair in a ferret (Mustela putorius furo) / Tratamento de fratura femoral em ferret (Mustela putorius furo)

Background: Ferrets (Mustela putorius furo) are increasingly popular as pets. They require similar medical and surgical care as small animals, and orthopedic lesions are more and more common. Fracture diagnosis is based on history, orthopedic exam, and diagnostic imaging. The preferred treatment for femur fractures is surgery, which has a favorable outcome in the majority of cases. However, surgical fracture repair in ferrets can be challenging due to the small size of the bones. The objective of this paper is to report the surgical repair of a femoral fracture in a ferret using an external skeletal fixation system type IA connected to an intramedullary pin (tie-in configuration). Case: An adult ferret was referred to the veterinary hospital after being stepped on. The ferret was diagnosed with an oblique diaphyseal fracture of the left femur and surgery was recommended. Open fracture reduction was performed with a 1 mm intramedullary pin (Kirschner wire) placed in a retrograde direction into the proximal bone segment. After fracture reduction, the intramedullary pin was inserted into the distal bone segment. The external skeletal fixator type IA was created by the insertion of a 1.5 mm Schanz pin into the distal diaphysis and another, also 1.5 mm, inserted into the proximal metaphysis, both percutaneously. The three pins were connected externally to an aluminum bar with the [...](AU)

Femoral fracture repair in a ferret (Mustela putorius furo) / Tratamento de fratura femoral em ferret (Mustela putorius furo)

Background: Ferrets (Mustela putorius furo) are increasingly popular as pets. They require similar medical and surgical care as small animals, and orthopedic lesions are more and more common. Fracture diagnosis is based on history, orthopedic exam, and diagnostic imaging. The preferred treatment for femur fractures is surgery, which has a favorable outcome in the majority of cases. However, surgical fracture repair in ferrets can be challenging due to the small size of the bones. The objective of this paper is to report the surgical repair of a femoral fracture in a ferret using an external skeletal fixation system type IA connected to an intramedullary pin (tie-in configuration). Case: An adult ferret was referred to the veterinary hospital after being stepped on. The ferret was diagnosed with an oblique diaphyseal fracture of the left femur and surgery was recommended. Open fracture reduction was performed with a 1 mm intramedullary pin (Kirschner wire) placed in a retrograde direction into the proximal bone segment. After fracture reduction, the intramedullary pin was inserted into the distal bone segment. The external skeletal fixator type IA was created by the insertion of a 1.5 mm Schanz pin into the distal diaphysis and another, also 1.5 mm, inserted into the proximal metaphysis, both percutaneously. The three pins were connected externally to an aluminum bar with the [...]

Ocorrência de Dioctophyma renale em Galictis cuja / Occurrence of Dioctophyma renale in Galictis cuja

A dioctofimose é uma parasitose causada pelo Dioctophyma renale (Goeze, 1782) de ocorrência mundial e acomete animais domésticos e silvestres. Em março de 2010, um exemplar adulto (macho) de Galictis cuja (Molina, 1782), encontrado morto por atropelamento no município de Guaíra, Paraná foi encaminhado ao laboratório de Patologia Veterinária de Universidade Federal do Paraná (UFPR), Campus Palotina, para a realização da necropsia. O cadáver apresentava bom estado nutricional e autólise moderada. Foram observados três exemplares de parasitos na cavidade abdominal, mas os rins encontravam-se preservados. Os parasitos foram fixados em formol acético e encaminhados ao laboratório de Parasitologia Veterinária da UFPR para identificação. Os exemplares coletados foram identificados como Dioctophyma renale, sendo duas fêmeas, uma com 39cm de comprimento por 4mm de largura e a outra com 16cm de comprimento por 4mm de largura e, um macho com 16cm de comprimento por 3mm de largura. O presente trabalho relata a ocorrência de parasitismo por D. renale em G. cuja na região oeste do estado do Paraná.

Dioctophymosis is a parasitic disease caused by Dioctophyma renale (Goeze, 1782) with a worldwide occurrence and affects domestic animals as well as wildlife. In March 2010, a ferret adult male, Galictis cuja (Molina, 1782), found dead by trampling in the county of Guaíra, state of Paraná, Brazil, was necropsied in the Veterinary Pathology Laboratory at Campus Palotina, Federal University of Paraná. The animal was in good nutritional condition and moderate autolysis. Three specimens of parasites were found in the abdominal cavity, but the kidneys were preserved. The parasites were fixed in acetic formaldehyde and sent to the Veterinary Laboratory of Parasitology, Campus Palotina, for identification. The parasites were identified as Dioctophyma renale, two females, one a 39cm long and 4mm wide and the other 16cm long and 4mm wide, and a male 16cm long and 3mm wide. This paper reports D. renale parasitism in G. cuja in the western of Paraná state, Brazil.

Ocorrência de Dioctophyma renale em Galictis cuja / Occurrence of Dioctophyma renale in Galictis cuja

A dioctofimose é uma parasitose causada pelo Dioctophyma renale (Goeze, 1782) de ocorrência mundial e acomete animais domésticos e silvestres. Em março de 2010, um exemplar adulto (macho) de Galictis cuja (Molina, 1782), encontrado morto por atropelamento no município de Guaíra, Paraná foi encaminhado ao laboratório de Patologia Veterinária de Universidade Federal do Paraná (UFPR), Campus Palotina, para a realização da necropsia. O cadáver apresentava bom estado nutricional e autólise moderada. Foram observados três exemplares de parasitos na cavidade abdominal, mas os rins encontravam-se preservados. Os parasitos foram fixados em formol acético e encaminhados ao laboratório de Parasitologia Veterinária da UFPR para identificação. Os exemplares coletados foram identificados como Dioctophyma renale, sendo duas fêmeas, uma com 39cm de comprimento por 4mm de largura e a outra com 16cm de comprimento por 4mm de largura e, um macho com 16cm de comprimento por 3mm de largura. O presente trabalho relata a ocorrência de parasitismo por D. renale em G. cuja na região oeste do estado do Paraná.(AU)

Dioctophymosis is a parasitic disease caused by Dioctophyma renale (Goeze, 1782) with a worldwide occurrence and affects domestic animals as well as wildlife. In March 2010, a ferret adult male, Galictis cuja (Molina, 1782), found dead by trampling in the county of Guaíra, state of Paraná, Brazil, was necropsied in the Veterinary Pathology Laboratory at Campus Palotina, Federal University of Paraná. The animal was in good nutritional condition and moderate autolysis. Three specimens of parasites were found in the abdominal cavity, but the kidneys were preserved. The parasites were fixed in acetic formaldehyde and sent to the Veterinary Laboratory of Parasitology, Campus Palotina, for identification. The parasites were identified as Dioctophyma renale, two females, one a 39cm long and 4mm wide and the other 16cm long and 4mm wide, and a male 16cm long and 3mm wide. This paper reports D. renale parasitism in G. cuja in the western of Paraná state, Brazil.(AU)

Anatomia ultrassonográfica dos linfonodos abdominais de furões europeus hígidos / Ultrasonographic anatomy of abdominal lymph nodes in the normal ferret

Nos últimos anos o furão (Mustela putorius furo) tornou-se um conhecido animal de estimação sendo observada uma população em constante crescimento no Brasil, e por conseqüência cada vez mais presente em clínicas veterinárias. O presente trabalho teve como objetivo avaliar a anatomia ultrassonográfica dos linfonodos abdominais de furões-europeus hígidos. Foram utilizados 20 animais, dentre os quais nove eram machos e onze fêmeas, com idade média total de três anos. Localizaram-se em 100 por cento dos furões os linfonodos mesentéricos, em 55 por cento dos animais os linfonodos pancreático-duodenal e esplênico, em 20 por cento o linfonodo gástrico e em 5 por cento o linfonodo hepático. Conclui-se que a localização e características ultrassonográfica dos linfonodos abdominais em furões são muito similares aos linfonodos abdominais de gatos, sendo este estudo uma orientação preliminar para a localização dos linfonodos abdominais de furões hígidos.(AU)

In recent years the ferret (Mustela putorius furo) has become popular pet featuring a ever growing population in Brazil, and being increasingly found in veterinary clinics. This study aimed to evaluate the ultrasound anatomy of abdominal lymph nodes of healthy ferrets. We used 20 animals, nine of which males and eleven females, with overall mean age of three years. We located the mesenteric lymph nodes in 100 percent of the ferrets, the pancreatic-duodenal lymph nodes and the splenic ones in 55 percent, the gastric lymph node in 20 percent, and the hepatic lymph node in 5 percent. We measured their lengths. It is concluded that the location and sonographic characteristics of the abdominal lymph nodes in ferrets are very similar to the abdominal lymph nodes of cats. This is a preliminary orientation for the location of abdominal lymph nodes of healthy ferrets.(AU)

Anatomia ultrassonográfica dos linfonodos abdominais de furões europeus hígidos / Ultrasonographic anatomy of abdominal lymph nodes in the normal ferret

Nos últimos anos o furão (Mustela putorius furo) tornou-se um conhecido animal de estimação sendo observada uma população em constante crescimento no Brasil, e por conseqüência cada vez mais presente em clínicas veterinárias. O presente trabalho teve como objetivo avaliar a anatomia ultrassonográfica dos linfonodos abdominais de furões-europeus hígidos. Foram utilizados 20 animais, dentre os quais nove eram machos e onze fêmeas, com idade média total de três anos. Localizaram-se em 100 por cento dos furões os linfonodos mesentéricos, em 55 por cento dos animais os linfonodos pancreático-duodenal e esplênico, em 20 por cento o linfonodo gástrico e em 5 por cento o linfonodo hepático. Conclui-se que a localização e características ultrassonográfica dos linfonodos abdominais em furões são muito similares aos linfonodos abdominais de gatos, sendo este estudo uma orientação preliminar para a localização dos linfonodos abdominais de furões hígidos.

In recent years the ferret (Mustela putorius furo) has become popular pet featuring a ever growing population in Brazil, and being increasingly found in veterinary clinics. This study aimed to evaluate the ultrasound anatomy of abdominal lymph nodes of healthy ferrets. We used 20 animals, nine of which males and eleven females, with overall mean age of three years. We located the mesenteric lymph nodes in 100 percent of the ferrets, the pancreatic-duodenal lymph nodes and the splenic ones in 55 percent, the gastric lymph node in 20 percent, and the hepatic lymph node in 5 percent. We measured their lengths. It is concluded that the location and sonographic characteristics of the abdominal lymph nodes in ferrets are very similar to the abdominal lymph nodes of cats. This is a preliminary orientation for the location of abdominal lymph nodes of healthy ferrets.

T-cell lymphoma in a ferret (Mustela putorius furo)

A 12 years old ferret was attended with clinical history of prostration and anorexia. The animal died a few hours after consultation. After necropsy, a histopathological exam of a liver tumor was carried out. The morphologic diagnosis was consistent with multicentric lymphoma. Immunohistochemical exam of the neoplastic cells was made additionally, and confirmed the diagnosis of T-cell lymphoma.

Occurrence of ferret enteric coronavirus in Brazil (Preliminary Report) / Ocorrência de coronavírus entérico de ferrets no Brasil - Nota Prévia

Ferret enteric coronavirus (FECV) is associated to the epizootic catarrhal enteritis (ECE) in ferrets (Mustela putorius furo). In this study, we report the occurrence of this agent in four diarrheic stool samples of domestic ferrets, analyzed by negative staining transmission electron microscopy and a specific RT-PCR assay targeting the nucleocapsid (N) gene. These findings are the first report of FECV in Brazil and address the importance of this virus on the etiology of enteric disorders in ferrets.

Coronavírus entérico de furões (FECV) é associado à enterite catarral epizoótica (ECE) em furões (Mustela putorius furo). Neste estudo, relatamos a ocorrência deste agente em quatro amostras fecais diarreicas de furões domésticos, analisadas por microscopia eletrônica de transmissão (contrastação negativa) e RT-PCR específica e direcionada ao gene de nucleocapsídeo (N). Estes achados constituem o primeiro relato de FECV no Brasil e remetem para a importância deste vírus na etiologia de quadros entéricos nestes animais.

Occurrence of ferret enteric coronavirus in Brazil (Preliminary Report) / Ocorrência de coronavírus entérico de ferrets no Brasil - Nota Prévia

Ferret enteric coronavirus (FECV) is associated to the epizootic catarrhal enteritis (ECE) in ferrets (Mustela putorius furo). In this study, we report the occurrence of this agent in four diarrheic stool samples of domestic ferrets, analyzed by negative staining transmission electron microscopy and a specific RT-PCR assay targeting the nucleocapsid (N) gene. These findings are the first report of FECV in Brazil and address the importance of this virus on the etiology of enteric disorders in ferrets.(AU)

Coronavírus entérico de furões (FECV) é associado à enterite catarral epizoótica (ECE) em furões (Mustela putorius furo). Neste estudo, relatamos a ocorrência deste agente em quatro amostras fecais diarreicas de furões domésticos, analisadas por microscopia eletrônica de transmissão (contrastação negativa) e RT-PCR específica e direcionada ao gene de nucleocapsídeo (N). Estes achados constituem o primeiro relato de FECV no Brasil e remetem para a importância deste vírus na etiologia de quadros entéricos nestes animais.(AU)

T-cell lymphoma in a ferret (Mustela putorius furo)

A 12 years old ferret was attended with clinical history of prostration and anorexia. The animal died a few hours after consultation. After necropsy, a histopathological exam of a liver tumor was carried out. The morphologic diagnosis was consistent with multicentric lymphoma. Immunohistochemical exam of the neoplastic cells was made additionally, and confirmed the diagnosis of T-cell lymphoma.(AU)

Sebaceous epithelioma in a ferret (Mustela putorius furo)

An eight years-old male ferret presented a ventral abdominal nodular mass near the inguinal region. On clinical examination, a warty nodular mass in the abdomen was detected and the ferret was submitted to excisional biopsy by surgical procedure. At histopathology, adenomatous structures with preponderance of basophilic reserve cells, some sebocytes and showing squamous differentiation were observed. The diagnosis of sebaceous epithelioma was established based on clinical presentation and histopathologic findings. The animal recovered and at the moment no complication was reported.

Sebaceous epithelioma in a ferret (Mustela putorius furo)

An eight years-old male ferret presented a ventral abdominal nodular mass near the inguinal region. On clinical examination, a warty nodular mass in the abdomen was detected and the ferret was submitted to excisional biopsy by surgical procedure. At histopathology, adenomatous structures with preponderance of basophilic reserve cells, some sebocytes and showing squamous differentiation were observed. The diagnosis of sebaceous epithelioma was established based on clinical presentation and histopathologic findings. The animal recovered and at the moment no complication was reported.