Intraspecific trait variation and the leaf economics spectrum across resource gradients and levels of organization.

Understanding patterns of functional trait variation across environmental gradients offers an opportunity to increase inference in the mechanistic causes of plant community assembly. The leaf economics spectrum (LES) predicts global tradeoffs in leaf traits and trait-environment relationships, but few studies have examined whether these predictions hold across different levels of organization, particularly within species. Here, we asked (1) whether the main assumptions of the LES (expected trait relationships and shifts in trait values across resource gradients) hold at the intraspecific level, and (2) how within-species trait correlations scale up to interspecific or among-community levels. We worked with leaf traits of saplings of woody species growing across light and soil N and P availability gradients in temperate rainforests of southern Chile. We found that ITV accounted for a large proportion of community-level variation in leaf traits (e.g., LMA and leaf P) and played an important role in driving community-level shifts in leaf traits across environmental gradients. Additionally, intraspecific leaf trait relationships were generally consistent with interspecific and community-level trait relationships and with LES predictions-e.g., a strong negative intraspecific LMA-leaf N correlation-although, most trait relationships varied significantly among species, suggesting idiosyncrasies in the LES at the intraspecific level.

Convergence in relationships between leaf traits, spectra and age across diverse canopy environments and two contrasting tropical forests.

Leaf age structures the phenology and development of plants, as well as the evolution of leaf traits over life histories. However, a general method for efficiently estimating leaf age across forests and canopy environments is lacking. Here, we explored the potential for a statistical model, previously developed for Peruvian sunlit leaves, to consistently predict leaf ages from leaf reflectance spectra across two contrasting forests in Peru and Brazil and across diverse canopy environments. The model performed well for independent Brazilian sunlit and shade canopy leaves (R2  = 0.75-0.78), suggesting that canopy leaves (and their associated spectra) follow constrained developmental trajectories even in contrasting forests. The model did not perform as well for mid-canopy and understory leaves (R2  = 0.27-0.29), because leaves in different environments have distinct traits and trait developmental trajectories. When we accounted for distinct environment-trait linkages - either by explicitly including traits and environments in the model, or, even better, by re-parameterizing the spectra-only model to implicitly capture distinct trait-trajectories in different environments - we achieved a more general model that well-predicted leaf age across forests and environments (R2  = 0.79). Fundamental rules, linked to leaf environments, constrain the development of leaf traits and allow for general prediction of leaf age from spectra across species, sites and canopy environments.

Seedling growth and survival of five tree species in secondary forests and adjacent pastures in the montane rain forests of Southern Costa Rica / Crecimiento y supervivencia de plántulas de cinco especies de árboles en bosques secundarios y pastizales adyacentes en un bosque lluvioso montano del sur de Costa Rica

Abstract:Successful forest restoration in tropical environments is limited by the paucity of studies on the initial establishment and early survival requirements of seedlings of most native tropical tree species under disturbed conditions. Here, we evaluated the initial growth responses and the regeneration potential of seedlings of five tree species native to Costa Rica (Hasseltia floribunda, Inga densiflora, Persea americana, Tapirira mexicana and Trichilia pittieri). Seedlings were grown in secondary forests and adjacent open pastures under contrasting conditions of light availability. We quantified seedling growth, survival and herbivory from August 2010 to August 2011 on a monthly basis, and measured differences in leaf mass per area (LMA) at the end of the experiment. We found significant variation in growth responses between the understory of secondary forests and pastures. Growth in height was highest in pastures across all species, with I. densiflora, P. americana and T. mexicana showing the most striking differences. In contrast, H. floribunda and T. pittieri did not show differences in diameter growth between environments. Except for T. mexicana, herbivory increased throughout the experiment in all the species. Herbivory increased faster in pastures for H. floribunda and T. pittieri and showed higher rates in the forest understory for I. densiflora and P. americana. Seedling survival showed significant differences for all species across environments. Survival of H. floribunda and I. densiflora was higher in secondary forests, whereas the other species showed higher survival in pastures. LMA showed higher values in the pastures across all species, showing that individuals exposed to high light conditions had great photosynthetic rate and great leaf construction capacity. Due to their rapid growth and high survival, I. densiflora and T. mexicana showed great potential to restore abandoned pastures and secondary forests. Increasing our knowledge on the response of seedlings under disturbed conditions in tropical ecosystems is critical for improving the restoration of altered environments by matching the ecological amplitude of native species with specific environmental conditions. Rev. Biol. Trop. 64 (4): 1565-1583. Epub 2016 December 01.

Resumen:La restauración exitosa del bosque en ambientes tropicales está limitada por la carencia de estudios sobre los requisitos de hábitat, el establecimiento inicial, y la sobrevivencia de las plántulas de la mayoría de las especies de árboles tropicales nativos. En este estudio, evaluamos las respuestas en el crecimiento inicial y el potencial de regeneración de las plántulas de cinco especies de árboles nativos de Costa Rica (Hasseltia floribunda, Inga densiflora, Persea americana, Tapirira mexicana y Trichilia pittieri). Sembramos las plántulas en bosques secundarios y en pastizales abiertos adyacentes con condiciones de luz contrastantes. Cuantificamos el crecimiento, la supervivencia y el daño por herbivoría de las plántulas de forma mensual entre agosto 2010 y agosto 2011, y medimos las diferencias en el la masa foliar por área de la hoja al final del experimento. Existió una variación significativa en el crecimiento de las plántulas expuestas a la sombra en los bosques secundarios y aquellas que crecieron en pastizales. El crecimiento en altura fue mayor en pastizales en todas las especies; I. densiflora, P. americana y T. mexicana mostraron la mayor diferencia entre ambientes. En con- traste, H. floribunda y T. pittieri no mostraron diferencias en el crecimiento del diámetro entre ambientes. Con la excepción de T. mexicana, todas las especies mostraron un aumento en herbivoría durante el estudio. En los pastizales, el incremento de la herbivoría fue más rápido en H. floribunda y T. pittieri, mientras que para I. densiflora y P. americana fue mayor en el bosque. La sobrevivencia de plántulas fue diferente entre ambientes para todas las especies. La sobrevivencia de H. floribunda e I. densiflora fue mayor en el bosque secundario, mientras que en las demás especies hubo mayor sobrevivencia de plántulas en los pastizales. La masa foliar por área fue mayor en las plántulas que crecieron en los pastizales que en bosque secundario para todas las especies, lo que sugiere que los individuos que crecieron en condiciones de alta incidencia de luz tuvieron mayor tasa fotosintética y mayor capacidad de construcción de la hoja. Debido al rápido crecimiento y alta supervivencia I. densiflora y T. mexicana tienen gran potencial para la restauración de bosques secundarios y pastizales abandonados. Generar información sobre la respuesta de plántulas creciendo en bosques secundarios y pastizales en regeneración es crítico para mejorar los procesos de restauración de ambientes alterados. La restauración de estos bosques depende de la correspondencia entre las condiciones específicas de hábitat y la plasticidad ecológica de las especies nativas.

Long-term effect of carbohydrate reserves on growth and reproduction of Prosopis denudans (Fabaceae): implications for conservation of woody perennials.

Prosopis denudans, an extreme xerophyte shrub, is consumed by ungulates and threatened by firewood gathering, because it is one of the preferred species used by Mapuche indigenous people of Patagonia. In a scenario of uncontrolled use of vegetation, it is very difficult to develop a conservation plan that jointly protects natural resources and its users. We performed a field experiment to assess the impact of defoliation on growth, reproduction and stores of a wild population of P. denudans. We imposed four levels of defoliation (removal of 100, 66, 33 and 0% of leaves) and evaluated the short- and long-term (3 years) effects of this disturbance. Seasonal changes in shoot carbohydrates suggested that they support leaf-flush and blooming. Severely defoliated individuals also used root reserves to support growth and leaf-flush after clipping. Vegetative growth was not affected by defoliation history. Leaf mass area increased after the initial clipping, suggesting the development of structural defenses. The depletion of root reserves at the end of the first year affected inflorescence production the following spring. We conclude that P. denudans shrubs could lose up to one-third of their green tissues without affecting growth or inflorescence production. The removal of a higher proportion of leaves will diminish stores, which in turn, will reduce or completely prevent blooming and, therefore, fruit production the following seasons. Very few studies integrate conservation and plant physiology, and we are not aware, so far, of any work dealing with long-term plant carbon economy of a long-lived perennial shrub as an applied tool in conservation. These results might help the development of management strategies that consider both the use and the conservation of wild populations of P. denudans.

Do community-weighted mean functional traits reflect optimal strategies?

The notion that relationships between community-weighted mean (CWM) traits (i.e. plot-level trait values weighted by species abundances) and environmental conditions reflect selection towards locally optimal phenotypes is challenged by the large amount of interspecific trait variation typically found within ecological communities. Reconciling these contrasting patterns is a key to advancing predictive theories of functional community ecology. We combined data on geographical distributions and three traits (wood density, leaf mass per area and maximum height) of 173 tree species in Puerto Rico. We tested the hypothesis that species are more likely to occur where their trait values are more similar to the local CWM trait values (the'CWM-optimality' hypothesis) by comparing species occurrence patterns (as a proxy for fitness) with the functional composition of forest plots across a precipitation gradient. While 70% of the species supported CWM-optimality for at least one trait, nearly 25% significantly opposed it for at least one trait, thereby contributing to local functional diversity. The majority (85%) of species that opposed CWM-optimality did so only for one trait and few species opposed CWM-optimality in multivariate trait space. Our study suggests that constraints to local functional variation act more strongly on multivariate phenotypes than on univariate traits.

Salt effects on functional traits in model and in economically important Lotus species.

A common stress on plants is NaCl-derived soil salinity. Genus Lotus comprises model and economically important species, which have been studied regarding physiological responses to salinity. Leaf area ratio (LAR), root length ratio (RLR) and their components, specific leaf area (SLA) and leaf mass fraction (LMF) and specific root length (SRL) and root mass fraction (RMF) might be affected by high soil salinity. We characterised L. tenuis, L. corniculatus, L. filicaulis, L. creticus, L. burtii and L. japonicus grown under different salt concentrations (0, 50, 100 and 150 mm NaCl) on the basis of SLA, LMF, SRL and RMF using PCA. We also assessed effects of different salt concentrations on LAR and RLR in each species, and explored whether changes in these traits provide fitness benefit. Salinity (150 mm NaCl) increased LAR in L. burtii and L. corniculatus, but not in the remaining species. The highest salt concentration caused a decrease of RLR in L. japonicus Gifu, but not in the remaining species. Changes in LAR and RLR would not be adaptive, according to adaptiveness analysis, with the exception of SLA changes in L. corniculatus. PCA revealed that under favourable conditions plants optimise surfaces for light and nutrient acquisition (SLA and SRL), whereas at higher salt concentrations they favour carbon allocation to leaves and roots (LMF and RMF) in detriment to their surfaces. PCA also showed that L. creticus subjected to saline treatment was distinguished from the remaining Lotus species. We suggest that augmented carbon partitioning to leaves and roots could constitute a salt-alleviating mechanism through toxic ion dilution.

Insect herbivores associated with an evergreen tree Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) in a tropical dry forest / Insetos herbívoros associados a uma árvore sempre verde Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) em uma floresta tropical seca

Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) is a tree species found in Brazilian tropical dry forests that retain their leaves during the dry season. That being, we addressed the following question: i) How do insect diversity (sap-sucking and chewing), leaf herbivory and defensive traits (tannin and leaf sclerophylly) vary on the evergreen tree species G. marginata between seasons? The abundance of sap-sucking insects was higher in the dry season than in the rainy season. However, we did not verify any difference in the species richness and abundance of chewing insects between seasons. Leaf herbivory was higher in the rainy season, whereas leaf sclerophylly was higher in the dry season. However, herbivory was not related to sclerophylly. Insect herbivores likely decrease their folivory activity during the dry season due to life history patterns or changes in behaviour, possibly entering diapause or inactivity during this period. Therefore, G. marginata acts as a likely keystone species, serving as a moist refuge for the insect fauna during the dry season in tropical dry forest, and the presence of this evergreen species is crucial to conservation strategies of this threatened ecosystem.(AU)

Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) é uma árvore encontrada em florestas tropicais secas do Brasil que mantém as suas folhas durante a estação seca. Baseado neste fato, nós levantamos a seguinte questão: i) como a diversidade de insetos herbívoros (sugadores e mastigadores), herbivoria foliar e características defensivas (taninos e esclerofilia foliar) variam em G. marginata entre as estações seca e chuvosa. A abundância de insetos sugadores foi maior na estação seca, entretanto, a riqueza e abundância de insetos mastigadores não diferiram entre as estações. A herbivoria foliar foi maior na estação chuvosa, enquanto a esclerofilia foliar foi maior na estação seca. Não foi observado efeito significativo da esclerofilia sobre os níveis de herbivoria foliar. Insetos herbívoros reduzem a sua atividade de consumo foliar durante a estação seca, fato este devido à sua história natural e/ou mudança comportamental, provavelmente, entrando em diapausa durante este período. Nossos resultados demonstram que G. marginata atua como refúgio para fauna de insetos durante a estação seca, e que a presença desta espécie merece relevante destaque em estratégias de conservação deste ameaçado ecossistema.(AU)

Insect herbivores associated with an evergreen tree Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) in a tropical dry forest / Insetos herbívoros associados a uma árvore sempre verde Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) em uma floresta tropical seca

Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) is a tree species found in Brazilian tropical dry forests that retain their leaves during the dry season. That being, we addressed the following question: i) How do insect diversity (sap-sucking and chewing), leaf herbivory and defensive traits (tannin and leaf sclerophylly) vary on the evergreen tree species G. marginata between seasons? The abundance of sap-sucking insects was higher in the dry season than in the rainy season. However, we did not verify any difference in the species richness and abundance of chewing insects between seasons. Leaf herbivory was higher in the rainy season, whereas leaf sclerophylly was higher in the dry season. However, herbivory was not related to sclerophylly. Insect herbivores likely decrease their folivory activity during the dry season due to life history patterns or changes in behaviour, possibly entering diapause or inactivity during this period. Therefore, G. marginata acts as a likely keystone species, serving as a moist refuge for the insect fauna during the dry season in tropical dry forest, and the presence of this evergreen species is crucial to conservation strategies of this threatened ecosystem.

Goniorrhachis marginata Taub. (Leguminosae: Caesalpinioideae) é uma árvore encontrada em florestas tropicais secas do Brasil que mantém as suas folhas durante a estação seca. Baseado neste fato, nós levantamos a seguinte questão: i) como a diversidade de insetos herbívoros (sugadores e mastigadores), herbivoria foliar e características defensivas (taninos e esclerofilia foliar) variam em G. marginata entre as estações seca e chuvosa. A abundância de insetos sugadores foi maior na estação seca, entretanto, a riqueza e abundância de insetos mastigadores não diferiram entre as estações. A herbivoria foliar foi maior na estação chuvosa, enquanto a esclerofilia foliar foi maior na estação seca. Não foi observado efeito significativo da esclerofilia sobre os níveis de herbivoria foliar. Insetos herbívoros reduzem a sua atividade de consumo foliar durante a estação seca, fato este devido à sua história natural e/ou mudança comportamental, provavelmente, entrando em diapausa durante este período. Nossos resultados demonstram que G. marginata atua como refúgio para fauna de insetos durante a estação seca, e que a presença desta espécie merece relevante destaque em estratégias de conservação deste ameaçado ecossistema.

Functional relationships between leaf hydraulics and leaf economic traits in response to nutrient addition in subtropical tree species.

Leaves can be both a hydraulic bottleneck and a safety valve against hydraulic catastrophic dysfunctions, and thus changes in traits related to water movement in leaves and associated costs may be critical for the success of plant growth. A 4-year fertilization experiment with nitrogen (N) and phosphorus (P) addition was done in a semideciduous Atlantic forest in northeastern Argentina. Saplings of five dominant canopy species were grown in similar gaps inside the forests (five control and five N + P addition plots). Leaf lifespan (LL), leaf mass per unit area (LMA), leaf and stem vulnerability to cavitation, leaf hydraulic conductance (K(leaf_area) and K(leaf_mass)) and leaf turgor loss point (TLP) were measured in the five species and in both treatments. Leaf lifespan tended to decrease with the addition of fertilizers, and LMA was significantly higher in plants with nutrient addition compared with individuals in control plots. The vulnerability to cavitation of leaves (P50(leaf)) either increased or decreased with the nutrient treatment depending on the species, but the average P50(leaf) did not change with nutrient addition. The P50(leaf) decreased linearly with increasing LMA and LL across species and treatments. These trade-offs have an important functional significance because more expensive (higher LMA) and less vulnerable leaves (lower P50(leaf)) are retained for a longer period of time. Osmotic potentials at TLP and at full turgor became more negative with decreasing P50(leaf) regardless of nutrient treatment. The K(leaf) on a mass basis was negatively correlated with LMA and LL, indicating that there is a carbon cost associated with increased water transport that is compensated by a longer LL. The vulnerability to cavitation of stems and leaves were similar, particularly in fertilized plants. Leaves in the species studied may not function as safety valves at low water potentials to protect the hydraulic pathway from water stress-induced cavitation. The lack of rainfall seasonality in the subtropical forest studied probably does not act as a selective pressure to enhance hydraulic segmentation between leaves and stems.

Características do pasto e eficiência agronômica de nitrogênio em capim-tanzânia sob pastejo contínuo, adubado com doses de nitrogênio / Sward characteristics and agronomic efficiency of nitrogen on Tanzania grass under continuous grazing fertilized with nitrogen levels

O experimento foi conduzido visando a avaliar os efeitos das doses de nitrogênio (N) 100, 200, 300 e 400kg ha-1 sobre as características do pasto e sobre a eficiência agronômica de N, em pastagens de capim-tanzânia (Panicum maximum Jacq. cv. ‘Tanzânia-1’) utilizadas com o método de pastejo contínuo. O delineamento experimental foi o inteiramente ao acaso, com duas repetições. Foram utilizados três tourinhos testadores nas pastagens com a dose de N de 100kg ha-1 e quatro tourinhos testadores nas pastagens das demais doses de N, da raça Nelore (Bos indicus) e com peso médio inicial de 300kg, mantidos de 10/11/2001 a 15/4/2002. A altura do pasto foi mantida em 60 cm por ajustes da taxa de lotação. As massas de forragem, de folha verde, de forragem verde e de colmo elevaram-se linearmente com o aumento da adubação de N. Regressão linear negativa foi verificada entre as doses de N e a razão folha:colmo. A participação de colmo se elevou e a de material morto se reduziu na estrutura do pasto com o aumento da adubação de N. Não houve efeito das doses de N na proporção de folha verde na estrutura do pasto. Aplicações de N em pastagens de capim-tanzânia utilizadas com o método de pastejo contínuo alteram características do pasto. A eficiência agronômica de N, definida em termos da razão acúmulo de matéria seca (MS) kg-1 de N aplicado, reduziu-se com o aumento da dose de N.

The experiment evaluated the effects of nitrogen (N) levels (100, 200, 300, 400kg ha-1) on sward characteristics, and agronomic efficiency of N on Tanzania grass pastures (Panicum maximum Jacq. cv. ‘Tanzânia-1’) with continuous grazing method. The design experimental used was completely randomized with two replications. Three tester Nellore (Bos indicus) young bulls per pasture with N level of 100kg ha-1, and four tester Nellore young bulls per pasture of the other N levels, with 300kg initial weight grazed continuously from 10 November 2001 to 15 April 2002. Sward height levels at experimental pastures were maintaining at 60cm with adjusting of stocking rate. The herbage mass, green leaf mass, green herbage mass, and culm mass, increased linearly as the N fertilization increased. Negatively linear relationship was found between the N fertilization and leaf/culm ratio. The culm proportion increased and dead material decreased on sward structure with increased N levels applied at pasture. There were no effects of N levels on green leaf proportion at sward structure. N applications in Tanzania grass pastures change sward characteristics. The agronomic efficiency of N defined in terms of dry matter (DM) accumulation/kg of N applied decreased with the increase of N levels.

Características do pasto e eficiência agronômica de nitrogênio em capim-tanzânia sob pastejo contínuo, adubado com doses de nitrogênio

The experiment evaluated the effects of nitrogen (N) levels (100, 200, 300, 400kg ha-1) on sward characteristics, and agronomic efficiency of N on Tanzania grass pastures (Panicum maximum Jacq. cv. Tanzânia-1) with continuous grazing method. The design experimental used was completely randomized with two replications. Three tester Nellore (Bos indicus) young bulls per pasture with N level of 100kg ha-1, and four tester Nellore young bulls per pasture of the other N levels, with 300kg initial weight grazed continuously from 10 November 2001 to 15 April 2002. Sward height levels at experimental pastures were maintaining at 60cm with adjusting of stocking rate. The herbage mass, green leaf mass, green herbage mass, and culm mass, increased linearly as the N fertilization increased. Negatively linear relationship was found between the N fertilization and leaf/culm ratio. The culm proportion increased and dead material decreased on sward structure with increased N levels applied at pasture. There were no effects of N levels on green leaf proportion at sward structure. N applications in Tanzania grass pastures change sward characteristics. The agronomic efficiency of N defined in terms of dry matter (DM) accumulation/kg of N applied decreased with the increase of N levels.

O experimento foi conduzido visando a avaliar os efeitos das doses de nitrogênio (N) 100, 200, 300 e 400kg ha-1 sobre as características do pasto e sobre a eficiência agronômica de N, em pastagens de capim-tanzânia (Panicum maximum Jacq. cv. Tanzânia-1) utilizadas com o método de pastejo contínuo. O delineamento experimental foi o inteiramente ao acaso, com duas repetições. Foram utilizados três tourinhos testadores nas pastagens com a dose de N de 100kg ha-1 e quatro tourinhos testadores nas pastagens das demais doses de N, da raça Nelore (Bos indicus) e com peso médio inicial de 300kg, mantidos de 10/11/2001 a 15/4/2002. A altura do pasto foi mantida em 60 cm por ajustes da taxa de lotação. As massas de forragem, de folha verde, de forragem verde e de colmo elevaram-se linearmente com o aumento da adubação de N. Regressão linear negativa foi verificada entre as doses de N e a razão folha:colmo. A participação de colmo se elevou e a de material morto se reduziu na estrutura do pasto com o aumento da adubação de N. Não houve efeito das doses de N na proporção de folha verde na estrutura do pasto. Aplicações de N em pastagens de capim-tanzânia utilizadas com o método de pastejo contínuo alteram características do pasto. A eficiência agronômica de N, definida em termos da razão acúmulo de matéria seca (MS) kg-1 de N aplicado, reduziu-se com o aumento da dose de N.

Características do pasto e eficiência agronômica de nitrogênio em capim-tanzânia sob pastejo contínuo, adubado com doses de nitrogênio

The experiment evaluated the effects of nitrogen (N) levels (100, 200, 300, 400kg ha-1) on sward characteristics, and agronomic efficiency of N on Tanzania grass pastures (Panicum maximum Jacq. cv. Tanzânia-1) with continuous grazing method. The design experimental used was completely randomized with two replications. Three tester Nellore (Bos indicus) young bulls per pasture with N level of 100kg ha-1, and four tester Nellore young bulls per pasture of the other N levels, with 300kg initial weight grazed continuously from 10 November 2001 to 15 April 2002. Sward height levels at experimental pastures were maintaining at 60cm with adjusting of stocking rate. The herbage mass, green leaf mass, green herbage mass, and culm mass, increased linearly as the N fertilization increased. Negatively linear relationship was found between the N fertilization and leaf/culm ratio. The culm proportion increased and dead material decreased on sward structure with increased N levels applied at pasture. There were no effects of N levels on green leaf proportion at sward structure. N applications in Tanzania grass pastures change sward characteristics. The agronomic efficiency of N defined in terms of dry matter (DM) accumulation/kg of N applied decreased with the increase of N levels.

O experimento foi conduzido visando a avaliar os efeitos das doses de nitrogênio (N) 100, 200, 300 e 400kg ha-1 sobre as características do pasto e sobre a eficiência agronômica de N, em pastagens de capim-tanzânia (Panicum maximum Jacq. cv. Tanzânia-1) utilizadas com o método de pastejo contínuo. O delineamento experimental foi o inteiramente ao acaso, com duas repetições. Foram utilizados três tourinhos testadores nas pastagens com a dose de N de 100kg ha-1 e quatro tourinhos testadores nas pastagens das demais doses de N, da raça Nelore (Bos indicus) e com peso médio inicial de 300kg, mantidos de 10/11/2001 a 15/4/2002. A altura do pasto foi mantida em 60 cm por ajustes da taxa de lotação. As massas de forragem, de folha verde, de forragem verde e de colmo elevaram-se linearmente com o aumento da adubação de N. Regressão linear negativa foi verificada entre as doses de N e a razão folha:colmo. A participação de colmo se elevou e a de material morto se reduziu na estrutura do pasto com o aumento da adubação de N. Não houve efeito das doses de N na proporção de folha verde na estrutura do pasto. Aplicações de N em pastagens de capim-tanzânia utilizadas com o método de pastejo contínuo alteram características do pasto. A eficiência agronômica de N, definida em termos da razão acúmulo de matéria seca (MS) kg-1 de N aplicado, reduziu-se com o aumento da dose de N.

Nitrogenous compounds, phenolic compounds and morphological aspects of leaves: comparison of deciduous and semideciduous arboreal legumes

In general, perennial species contain higher concentrations of certain secondary compounds, such as phenolics, lower levels of nitrogenous compounds, and greater specific leaf mass (SLM) than deciduous species. The aim of this study was to verify whether the comparative data reported in the literature regarding deciduous and evergreen species, also applies to four selected species of a semideciduous tropical forest (a remnant of the Atlantic Forest). The four species chosen for this study, each with apparently different leaf life spans, were two semideciduous (Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang., and Lonchocarpus guilleminianus (Tul.) Malme, and two deciduous (Enterolobium contortisiliquum (Vell.) Morong., and Peltophorum dubium (Spreng.) Taub.). Plants were grown in a greenhouse in plastic pots containing soil taken from their natural habitat. Mature leaves were harvested for determination of the SLM and contents of phenolic compounds, tannins, nitrate, protein, free amino acids, chlorophyll, and nitrogen. Data registered are in agreement with those of the literature. Leaves with the longest life span had the highest content of phenolics and the lowest levels of tannins, nitrate, protein, amino acids, chlorophyll and nitrogen, as well as presenting the greatest SLM.

Em geral as espécies perenifólias possuem maior concentração de alguns compostos secundários, como por exemplo fenóis, menor concentração de compostos nitrogenados e maior massa foliar específica (MFE), quando comparadas a espécies decíduas. O objetivo deste trabalho foi verificar se as observações encontradas na literatura, comparando espécies perenifólias e decíduas aplicam-se também a quatro espécies de uma mata semidecídua, remanescente da Mata Atlântica. Das quatro espécies usadas neste estudo, que aparentemente apresentavam longevidades foliares diferentes, duas eram semidecíduas (Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang. e Lonchocarpus guilleminianus (Tul.) Malme) e duas decíduas (Enterolobium contortisiliquum (Vell.) Morong. e Peltophorum dubium (Spreng.) Taub.). As plantas foram cultivadas em vasos plásticos, contendo solo da mata. O cultivo foi feito em casa de vegetação. Folhas maduras foram coletadas para análise de fenóis totais, taninos condensados, nitrato, proteínas totais, aminoácidos livres totais, clorofila total, nitrogênio total e MFE. Os resultados obtidos corroboram os da literatura, já que as espécies com folhas mais longevas apresentaram concentrações maiores de fenóis totais, menores de taninos condensados, nitrato, proteínas solúveis totais, aminoácidos livres totais, clorofilas totais, nitrogênio total e maior MFE.

Nitrogenous compounds, phenolic compounds and morphological aspects of leaves: comparison of deciduous and semideciduous arboreal legumes

In general, perennial species contain higher concentrations of certain secondary compounds, such as phenolics, lower levels of nitrogenous compounds, and greater specific leaf mass (SLM) than deciduous species. The aim of this study was to verify whether the comparative data reported in the literature regarding deciduous and evergreen species, also applies to four selected species of a semideciduous tropical forest (a remnant of the Atlantic Forest). The four species chosen for this study, each with apparently different leaf life spans, were two semideciduous (Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang., and Lonchocarpus guilleminianus (Tul.) Malme, and two deciduous (Enterolobium contortisiliquum (Vell.) Morong., and Peltophorum dubium (Spreng.) Taub.). Plants were grown in a greenhouse in plastic pots containing soil taken from their natural habitat. Mature leaves were harvested for determination of the SLM and contents of phenolic compounds, tannins, nitrate, protein, free amino acids, chlorophyll, and nitrogen. Data registered are in agreement with those of the literature. Leaves with the longest life span had the highest content of phenolics and the lowest levels of tannins, nitrate, protein, amino acids, chlorophyll and nitrogen, as well as presenting the greatest SLM.

Em geral as espécies perenifólias possuem maior concentração de alguns compostos secundários, como por exemplo fenóis, menor concentração de compostos nitrogenados e maior massa foliar específica (MFE), quando comparadas a espécies decíduas. O objetivo deste trabalho foi verificar se as observações encontradas na literatura, comparando espécies perenifólias e decíduas aplicam-se também a quatro espécies de uma mata semidecídua, remanescente da Mata Atlântica. Das quatro espécies usadas neste estudo, que aparentemente apresentavam longevidades foliares diferentes, duas eram semidecíduas (Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang. e Lonchocarpus guilleminianus (Tul.) Malme) e duas decíduas (Enterolobium contortisiliquum (Vell.) Morong. e Peltophorum dubium (Spreng.) Taub.). As plantas foram cultivadas em vasos plásticos, contendo solo da mata. O cultivo foi feito em casa de vegetação. Folhas maduras foram coletadas para análise de fenóis totais, taninos condensados, nitrato, proteínas totais, aminoácidos livres totais, clorofila total, nitrogênio total e MFE. Os resultados obtidos corroboram os da literatura, já que as espécies com folhas mais longevas apresentaram concentrações maiores de fenóis totais, menores de taninos condensados, nitrato, proteínas solúveis totais, aminoácidos livres totais, clorofilas totais, nitrogênio total e maior MFE.

Leaf life span and nitrogen content in semideciduous forest tree species (Croton priscus and Hymenaea courbaril)

In comparison to deciduous species, evergreen plants have lower leaf nutrient contents and higher leaf life span, important mechanisms for nutrient economy, allowing the colonization of low fertility soils. Strategies to conserve nitrogen in two semideciduous tropical forest tree species, with different leaf life spans were analyzed. The hypothesis was the fact that the two species would present different nitrogen conservation mechanisms in relation to chemical (total nitrogen, protein, chlorophyll, and proteolytic activity), functional (leaf life span, N-use efficiency, and N-resorption efficiency), morphological (specific leaf mass) leaf characteristics, and total nitrogen in the soil. Hymenaea courbaril L. presented lower nitrogen compounds in leaves, longer leaf life span, higher N-use efficiency, and higher specific leaf mass, while absorbing proportionally less nitrogen from the soil than Croton priscus Croizat. These characteristics can contribute for a better nitrogen economy strategy of H. courbaril. No relationship was found between leaf life span and N resorption efficiency, nor between leaf life span, protease activity and nitrogen mobilization. The electrophoretic profiles of proteolytic enzymes in young leaves of the two species presented more bands with enzymatic activity than other kinds of leaves.

Comparadas a espécies decíduas, as sempre-verdes têm menos nutrientes nas folhas, que também são mais longevas. Estes mecanismos são importantes para economia de nutrientes, e permitem a colonização de solos com baixa fertilidade. Foram analisadas estratégias de conservação de nitrogênio em duas espécies de floresta semidecídua, que aparentemente apresentavam longevidades foliares diferentes. Para isto foram comparados mecanismos químicos (concentrações de nitrogênio total, de proteína e clorofila e atividade proteolítica), funcionais (duração de vida das folhas, eficiência do uso de nitrogênio e eficiência de redistribuição de nitrogênio) e morfológico (massa foliar específica) de conservação de nitrogênio nas folhas e a concentração de nitrogênio no solo. Hymenaea courbaril L. apresentou menor concentração de compostos nitrogenados nas folhas, maior longevidade foliar, maior eficiência do uso do nitrogênio e maior massa foliar específica, além de ter retirado proporcionalmente menos nitrogênio do solo do que Croton priscus Croizat. Estas características podem contribuir com uma melhor estratégia de conservação de nitrogênio em H. courbaril. Não foi encontrada relação entre a duração de vida da folha e a eficiência de redistribuição de nitrogênio, ou entre a duração de vida da folha, atividade de proteases e mobilização de nitrogênio. As folhas jovens das duas espécies apresentaram mais bandas com atividades enzimáticas nos perfís eletroforéticos de enzimas proteolíticas.

Leaf life span and nitrogen content in semideciduous forest tree species (Croton priscus and Hymenaea courbaril)

In comparison to deciduous species, evergreen plants have lower leaf nutrient contents and higher leaf life span, important mechanisms for nutrient economy, allowing the colonization of low fertility soils. Strategies to conserve nitrogen in two semideciduous tropical forest tree species, with different leaf life spans were analyzed. The hypothesis was the fact that the two species would present different nitrogen conservation mechanisms in relation to chemical (total nitrogen, protein, chlorophyll, and proteolytic activity), functional (leaf life span, N-use efficiency, and N-resorption efficiency), morphological (specific leaf mass) leaf characteristics, and total nitrogen in the soil. Hymenaea courbaril L. presented lower nitrogen compounds in leaves, longer leaf life span, higher N-use efficiency, and higher specific leaf mass, while absorbing proportionally less nitrogen from the soil than Croton priscus Croizat. These characteristics can contribute for a better nitrogen economy strategy of H. courbaril. No relationship was found between leaf life span and N resorption efficiency, nor between leaf life span, protease activity and nitrogen mobilization. The electrophoretic profiles of proteolytic enzymes in young leaves of the two species presented more bands with enzymatic activity than other kinds of leaves.

Comparadas a espécies decíduas, as sempre-verdes têm menos nutrientes nas folhas, que também são mais longevas. Estes mecanismos são importantes para economia de nutrientes, e permitem a colonização de solos com baixa fertilidade. Foram analisadas estratégias de conservação de nitrogênio em duas espécies de floresta semidecídua, que aparentemente apresentavam longevidades foliares diferentes. Para isto foram comparados mecanismos químicos (concentrações de nitrogênio total, de proteína e clorofila e atividade proteolítica), funcionais (duração de vida das folhas, eficiência do uso de nitrogênio e eficiência de redistribuição de nitrogênio) e morfológico (massa foliar específica) de conservação de nitrogênio nas folhas e a concentração de nitrogênio no solo. Hymenaea courbaril L. apresentou menor concentração de compostos nitrogenados nas folhas, maior longevidade foliar, maior eficiência do uso do nitrogênio e maior massa foliar específica, além de ter retirado proporcionalmente menos nitrogênio do solo do que Croton priscus Croizat. Estas características podem contribuir com uma melhor estratégia de conservação de nitrogênio em H. courbaril. Não foi encontrada relação entre a duração de vida da folha e a eficiência de redistribuição de nitrogênio, ou entre a duração de vida da folha, atividade de proteases e mobilização de nitrogênio. As folhas jovens das duas espécies apresentaram mais bandas com atividades enzimáticas nos perfís eletroforéticos de enzimas proteolíticas.

Produção e qualidade de folhas de amoreira em função da época do ano e de colheita

The amount and quality of mulberry (Morus alba L.) leaves have remarkable influence on the development of the silkworm and they are, therefore, influenced by factors as the age of the leaves, season and genetic characters of the cultivar. The production and the nutritional quality of leaves of five mulberry cultivars were evaluated under different seasons of the year and harvest times, having in view the indication of cultivars for the area of Jaboticabal, SP, Brazil. Leaves of cultivars 'Miura', 'Korin', 'Calabresa', 'FM86' and 'FM Shima-Miura' were harvested in spring, summer and autumn, 45, 60, 75 and 90 days after pruning (DAP) and evaluated through leaf mass production, crude protein (CP) and neutral detergent fiber (NDF). Best productions were verified in 'FM Shima-Miura', mainly at 75 DAP for spring and summer and at 90 DAP for autumn. For summer, the harvest made at 60, 75 and 90 DAP resulted in highest productions. Harvest times that resulted in largest CP levels were 45 and 60 DAP and in smallest NDF level was 60 DAP, respectively. It was possible to reunite high PB levels, low NDF levels and relatively high leaf mass productions only at the harvest time of 75 DAP.

A quantidade e qualidade das folhas de amoreira (Morus alba L.) exercem influência marcante sobre o desenvolvimento do bicho-da-seda e são, por sua vez, influenciadas por fatores como a idade das folhas, época do ano e características genéticas dos cultivares. Avaliou-se a produção e a qualidade nutricional de folhas de cinco cultivares de amoreira em diferentes épocas do ano e de colheita, objetivando verificar sua adaptação na região de Jaboticabal (SP). As folhas dos cultivares 'Miura', 'Korin', 'Calabresa', 'FM86' e 'FM Shima-Miura' foram colhidas na primavera, verão e outono após 45, 60, 75 e 90 dias da poda (DAP) e avaliadas quanto à produção de folhas níveis de proteína bruta (PB) e fibra em detergente neutro (FDN). As melhores produções foram verificadas em 'FM Shima-Miura', principalmente aos 75 DAP na primavera e no verão e aos 90 DAP no outono. No verão, as colheitas efetuadas aos 60, 75 e 90 DAP resultaram em produções mais elevadas. As épocas de colheita que resultaram em maiores níveis de PB foram aos 45 e 60 DAP e em menores níveis de FDN aos 60 DAP, respectivamente. Foi possível conciliar altos níveis de PB, baixos níveis de FDN e relativamente alta produção de massa foliar somente na colheita efetuada aos 75 DAP.

Produção e qualidade de folhas de amoreira em função da época do ano e de colheita

The amount and quality of mulberry (Morus alba L.) leaves have remarkable influence on the development of the silkworm and they are, therefore, influenced by factors as the age of the leaves, season and genetic characters of the cultivar. The production and the nutritional quality of leaves of five mulberry cultivars were evaluated under different seasons of the year and harvest times, having in view the indication of cultivars for the area of Jaboticabal, SP, Brazil. Leaves of cultivars 'Miura', 'Korin', 'Calabresa', 'FM86' and 'FM Shima-Miura' were harvested in spring, summer and autumn, 45, 60, 75 and 90 days after pruning (DAP) and evaluated through leaf mass production, crude protein (CP) and neutral detergent fiber (NDF). Best productions were verified in 'FM Shima-Miura', mainly at 75 DAP for spring and summer and at 90 DAP for autumn. For summer, the harvest made at 60, 75 and 90 DAP resulted in highest productions. Harvest times that resulted in largest CP levels were 45 and 60 DAP and in smallest NDF level was 60 DAP, respectively. It was possible to reunite high PB levels, low NDF levels and relatively high leaf mass productions only at the harvest time of 75 DAP.

A quantidade e qualidade das folhas de amoreira (Morus alba L.) exercem influência marcante sobre o desenvolvimento do bicho-da-seda e são, por sua vez, influenciadas por fatores como a idade das folhas, época do ano e características genéticas dos cultivares. Avaliou-se a produção e a qualidade nutricional de folhas de cinco cultivares de amoreira em diferentes épocas do ano e de colheita, objetivando verificar sua adaptação na região de Jaboticabal (SP). As folhas dos cultivares 'Miura', 'Korin', 'Calabresa', 'FM86' e 'FM Shima-Miura' foram colhidas na primavera, verão e outono após 45, 60, 75 e 90 dias da poda (DAP) e avaliadas quanto à produção de folhas níveis de proteína bruta (PB) e fibra em detergente neutro (FDN). As melhores produções foram verificadas em 'FM Shima-Miura', principalmente aos 75 DAP na primavera e no verão e aos 90 DAP no outono. No verão, as colheitas efetuadas aos 60, 75 e 90 DAP resultaram em produções mais elevadas. As épocas de colheita que resultaram em maiores níveis de PB foram aos 45 e 60 DAP e em menores níveis de FDN aos 60 DAP, respectivamente. Foi possível conciliar altos níveis de PB, baixos níveis de FDN e relativamente alta produção de massa foliar somente na colheita efetuada aos 75 DAP.