Genome-based reclassification of Kineococcus aureolus Xu et al. 2017 as a later heterotypic synonym of Kineococcus terrestris Xu et al. 2017.

We aimed to elucidate the relationship between Kineococcus terrestris and Kineococcus aureolus through whole-genome-based analysis. The genome-derived 16S rRNA gene sequences of K. terrestris KCTC 39738T and K. aureolus KCTC 39739T shared a 100% similarity. Phylogenetic trees based on 16S rRNA gene and whole-genome sequences revealed that K. terrestris KCTC 39738T and K. aureolus KCTC 39739T formed a robust clade, indicating a close relationship between them. Genomic comparison showed that the two strains shared 99.1% average nucleotide identity, 92.0% digital DNA-DNA hybridization and 98.9% average amino acid identity values, all of which exceeded the recommended threshold values for species classification. Most phenotypic characteristics between the two species were almost identical. Based on the above evidence, we propose the reclassification of Kineococcus aureolus Xu et al. 2017 as a later heterotypic synonym of Kineococcus terrestris Xu et al. 2017. Since these two species were proposed in the same article, the principle of priority does not apply. Our proposal is supported by the fact that the nomenclatural authorities first described Kineococcus terrestris.

Genome-based reclassification of Williamsia marianensis as a later heterotypic synonym of Williamsia muralis.

The present study used whole-genome data to clarify the taxonomic assignment of two closely related Williamsia species. Genomic information for 10 type strains was available at the time of conducting this analysis. One group of type strains was found to be conspecific, namely Williamsia muralis Kämpfer et al. 1999 and Williamsia marianensis Pathom-aree et al. 2006. The 16S rRNA gene sequences showed 99 % similarity between these type strains. Whole-genome-based comparisons showed that Williamsia muralis DSM 44343T and Williamsia marianensis DSM 44944T shared 98.07 % average nucleotide identity, 98.29 % average amino acid identity and 84.80 % digital DNA-DNA hybridization values. These values exceeded the threshold values for bacterial species delineation. Further, phylogenomic analysis based on the core genomes of the strains under study confirmed that Williamsia muralis DSM 44343T and Williamsia marianensis DSM 44944T formed a monophyletic clade. Based on this evidence, we propose the reclassification of Williamsia marianensis Pathom-aree et al. 2006 as a later heterotypic synonym of Williamsia muralis Kämpfer et al. 1999.

Genomic evidence that Ornithinicoccus soli Jiang et al. 2020 is a later heterotypic synonym of Segeticoccus rhizosphaerae Lee and Whang 2020.

The genus Segeticoccus includes only one species with validly published name, Segeticoccus rhizosphaerae. The type strain Segeticoccus rhizosphaerae YJ01T was isolated from soil in Korea, while Ornithinicoccus soli XNB-1T was isolated from soil in China. Both strains share similar phenotypic and chemotaxonomic characteristics, including predominant menaquinone MK-8(H4) and major polar lipids diphosphatidylglycerol and phosphatidylglycerol. Whole genome sequences revealed a DNA G+C content of 70.1 mol% for both strains and 100% similarity in their 16S rRNA gene sequences. Phylogenetic analysis showed they form a distinct cluster separate from other genera. Genomic comparisons showed average nucleotide identity and digital DNA-DNA hybridization values of 99.16 and 94.2%, respectively, indicating they represent a single species. Based on this genomic evidence, Ornithinicoccus soli Jiang et al. 2020 is proposed to be a later heterotypic synonym of Segeticoccus rhizosphaerae Lee and Whang 2020.

Taxonomic notes on the collection of the tribe Lamiini (Coleoptera, Cerambycidae) housed in the Natural History Museum, London.

In the present paper, the genus Mimomyagrus Breuning, 1970 is synonymized with Combe Thomson, 1864, and Mimomyagruspfanneri Breuning, 1970 is considered a junior synonym of Combebrianus (White, 1858). The female of Arctolamiasinica Bi & Chen, 2022 is described for the first time and this species is reported as new to Myanmar. Type material of Lamiapunctator Fabricius, 1776 [= Anoplophorachinensis (Forster, 1771)], Cerambyxgalloprovincialis Olivier, 1800 [= Monochamusgalloprovincialis (Olivier, 1800)] and Melanaustergranulipennis Breuning, 1938 [= Monochamusguerryi Pic, 1903] are confirmed to be preserved in Natural History Museum, London.

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 20: Suitability of taxonomic units notified to EFSA until March 2024.

The qualified presumption of safety (QPS) process was developed to provide a safety assessment approach for microorganisms intended for use in food or feed chains. In the period covered by this statement, no new information was found that would change the status of previously recommended QPS TUs. The TUs in the QPS list were updated based on a verification, against their respective authoritative databases, of the correctness of the names and completeness of synonyms. A new procedure has been established to ensure the TUs are kept up to date in relation to recent taxonomical insights. Of 83 microorganisms notified to EFSA between October 2023 and March 2024 (47 as feed additives, 25 as food enzymes or additives, 11 as novel foods), 75 were not evaluated because: 15 were filamentous fungi, 1 was Enterococcus faecium, 10 were Escherichia coli, 1 was a Streptomyces (all excluded from the QPS evaluation) and 48 were TUs that already have a QPS status. Two of the other eight notifications were already evaluated for a possible QPS status in the previous Panel Statement: Heyndrickxia faecalis (previously Weizmannia faecalis) and Serratia marcescens. One was notified at genus level so could not be assessed for QPS status. The other five notifications belonging to five TUs were assessed for possible QPS status. Akkermansia muciniphila and Actinomadura roseirufa were still not recommended for QPS status due to safety concerns. Rhizobium radiobacter can be recommended for QPS status with the qualification for production purposes. Microbacterium arborescens and Burkholderia stagnalis cannot be included in the QPS list due to a lack of body of knowledge for its use in the food and feed chain and for B. stagnalis also due to safety concerns. A. roseirufa and B. stagnalis have been excluded from further QPS assessment.

Notes on the genus Syzygium (Myrtaceae) from Cambodia, Thailand, Vietnam, China and Taiwan.

Syzygiumkampotense is a new species from Cambodia. Syzygiumcerasiforme, S.foxworthianum, and S.angkae and S.thorelii are new records from Cambodia, Vietnam and China, respectively. Syzygiumprainianumsubsp.minor and S.densinerviumvar.insulare are placed under S.prainianum and S.densinervium, respectively. Eugeniacochinchinensis, E.eburnea and E.ripicola, are reduced to synonymy under S.pellucidum. Lectotypes for Eugeniadensifloravar.angustifolia, E.pellucida and E.pellucidavar.contracta are designated.

A Synopsis of Dicranum Hedw. (Dicranaceae, Bryophyta) in China, with Special References to Four Species Newly Reported and Re-Evaluation of Dicranum psathyrum Klazenga.

Dicranum Hedw. is a highly diverse and widely distributed genus within Dicranaceae. The species diversity and distribution of this genus in China, however, remain not well known. A new revision of Dicranum in China using morphological and molecular phylogenetic methods confirms that China has 39 species, including four newly reported species, D. bardunovii Tubanova & Ignatova, D. dispersum Engelmark, D. schljakovii Ignatova & Tubanova, and D. spadiceum J.E.Zetterst. Dicranum psathyrum Klazenga is transferred to Dicranoloma (Renauld) Renauld as a new synonym of Dicranoloma fragile Broth. Two species, Dicranum brevifolium (Lindb.) Lindb. and D. viride (Sull. & Lesq.) Lindb. are excluded from the bryoflora of China. A key to the Chinese Dicranum species is also provided. These results indicate an underestimation of the distribution range of numerous Dicranum species, underscoring the need for further in-depth investigations into the worldwide Dicranum diversity.

Intricate Networks in Nomenclature: Cases of Naming in Arthrocaulon, Arthrocnemum, and Salicornia (Amaranthaceae).

The nomenclatural status and typification of the names Arthrocaulon macrostachyum, Salicornia fruticosa, S. fruticosa var. deflexa, S. fruticosa var. glaucescens, S. fruticosa var. intermedia, S. fruticosa var. humilis, S. fruticosa var. pachystachya, S. fruticulosa, S. glauca, S. lignosa, S. macrostachya var. virescens, S. macrostachya var. glaucescens, S. perennis, S. radicans, S. radicans var. caespitosa, S. sarmentosa, S. sempervirens, and S. virginica, as well as an unnamed ß-variety of S. fruticosa proposed by A. Bertoloni, are investigated. Concerning A. macrostachyum, we document that the type indicated in literature (G00177362) is not a holotype, and that lectotypification is necessary. A specimen from G (G00687638) is here designated as a lectotype. On the level of variety, Arthrocnemum fruticosum var. macrostachyum is an earlier legitimate name for Salicornia fruticosa var. pachystachya. Furthermore, Piirainen et al. are incorrect when citing Forsskål's "Salicornia" from Alexandria as "S. virginica Forssk."; it is not a new name and should be cited as S. virginica auct. non L., as published in Forsskål's Flora Aegyptiaco-Arabica. Like with numerous other parallel cases in Flora Aegyptiaco-Arabica, Forsskål's designation of "Salicornia virginica" for an Arabian plant is to be considered a misapplication of the earlier Linnaean name for an American plant. Arthrocnemum glaucum (a nomen illegitimum of Ungern-Sternberg), was listed as type species of Arthrocnemum by the Names in Current Use project; the basionym, Salicornia glauca Delile, is here lectotypified and identified as Arthrocaulon meridionale, published by Ramirez et al. Updated synonymies of Arthrocaulon macrostachyum, A. meridionale, Salicornia fruticosa, and S. perennis are proposed. Salicornia sempervirens is an invalid name according to Art. 36.1a of ICN. No original material was found for S. radicans var. caespitosa. This paper also refer to lecto- or neotypifications on specimens deposited at BM, G, LINN-HS, LY, MPU, NAP, and PAL, and their current taxonomic positions are suggested in a taxonomic part of the paper.

Genome-based reclassification of Sphingopyxis lutea Chhetri et al. 2023 as a later heterotypic synonym of Sphingopyxis jiangsuensis Gao et al. 2023.

Sphingopyxis lutea DHUNG17T was compared with Sphingopyxis jiangsuensis XHP0097T to examine the taxonomic relationship between the two type strains. The 16S rRNA gene sequence of S. lutea DHUNG17T shared high similarity (99.9 %) to that of S. jiangsuensis XHP0097T. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains formed a tight cluster within the genus Sphingopyxis. The average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between S. lutea DHUNG17T and S. jiangsuensis XHP0097T were below 99.0, 99.1 and 92.2±1.7 %, respectively, all of which were greater than the species delineation threshold for AAI (95.5 %), ANI (95-96 %) and dDDH (70 %), strongly indicating that the two strains represented a single species. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we propose Sphingopyxis lutea as a later heterotypic synonym of Sphingopyxis jiangsuensis.

Variation of the Tegmen and Cercus in Sinopodisma rostellocerca (Orthoptera: Acrididae: Melanoplinae) with Proposal of a New Synonym.

Intraspecific variation is ubiquitous from individual traits to population level and plays an important role in a variety of fields. However, it is often ignored by systematists and comparative evolutionary biologists. In view of the limited knowledge of intraspecific variation, morphology-based identification has hindered the recognition of species borders and led to a great number of problems in the field of taxonomy and systematics. In this study, the intraspecific variation of the tegmen and cercus in Sinopodisma rostellocerca was examined, the variation patterns were summarized and the relationship between S. rostellocerca and S. hengshanica was discussed. The results showed that the intraspecific variation in the tegmen and male cercus was mainly manifested in the length and shape of the apical margin and dorso- and ventro-apical angles; this substantial variation occurred not only among intrapopulation individuals but also between the different sides of the same individuals, and all types of variation in S. hengshanica fell into the range of variation in S. rostellocerca, leading to the disappearance of the boundary between the two species. Therefore, S. hengshanica was herein considered as a new junior synonym of S. rostellocerca.

Marortus luteolus Yu et al. 2019 is a later heterotypic synonym of Zhongshania marina On et al. 2019.

Phylogeny of 16S rRNA gene sequences showed that Marortus luteolus ZX-21T and Zhongshania marina DSW25-10T are closely related, and form a monophyletic clade affiliated with the genus Zhongshania. Whole genome sequence comparisons showed that M. luteolus ZX-21T and Z. marina DSW25-10T shared 78.8 % digital DNA-DNA hybridization, 97.6 % average nucleotide identity and 98.1 % average amino acid identity. These values exceeded the recommended threshold values for species delineation. Thus, based on the principle of priority, we propose the reclassification of Marortus luteolus Yu et al. 2019 as a later heterotypic synonym of Zhongshania marina On et al. 2019.

A new synonym of Rhododendronleishanicum (subg. Hymenanthes) from China (Ericaceae).

Based on a critical examination of type specimens, images of living plants, and the literature has shown Rhododendronoligocarpum to be conspecific with R.leishanicum. Although slight variations in corolla colour exist amongst different populations of R.oligocarpum, it does not serve as a key distinguishing trait. Therefore, we reduced R.oligocarpum to a synonym of R.leishanicum, and recommend placing it in Subsection Maculifera.

Revisions of the clavipes and pruni species groups of the genus Merodon Meigen, 1803 (Diptera, Syrphidae).

This study focuses on the avidus-nigritarsis lineage within the genus Merodon, exploring morphological, genetic, and distributional aspects of two related assemblies within this lineage: the clavipes and pruni species groups. An integrative taxonomic approach was followed to ensure comprehensive species identification and validation, using adult morphology, wing geometric morphometrics, and genetic analysis of the mtDNA COI gene. In the clavipes group, seven species were identified, including three new species: M.aenigmaticus Vujic, Radenkovic & Likov, sp. nov., M.latens Vujic, Radenkovic & Likov, sp. nov., and M.rufofemoris Vujic, Radenkovic & Likov, sp. nov. In the pruni group, our revision revealed a new species, M.aequalis Vujic, Radenkovic & Likov, sp. nov., and the revalidation of Merodonobscurus Gil Collado, 1929, stat. rev. Merodonpallidus Macquart, 1842 is redescribed. Diagnoses, identification keys to species, and distribution maps are provided, and neotypes for Syrphusclavipes Fabricius, 1781 and Merodonquadrinotatus (Sack, 1931) are designated. Additionally, the following new synonyms are proposed: M.clavipesalbus syn. nov., M.clavipesater syn. nov., M.clavipesniger syn. nov., and M.splendens syn. nov. are junior synonyms of M.clavipes; and M.veloxarmeniacus syn. nov. and M.veloxanathema syn. nov. are junior synonyms of M.velox.

Alseodaphnopsismaguanensis is conspecific with A.hokouensis (Lauraceae) based on morphological and molecular evidence.

Based on both morphological and molecular evidence, it is confirmed that Alseodaphnopsismaguanensis is conspecific with A.hokouensis. Hence, Alseodaphnopsismaguanensis is treated as a synonym of A.hokouensis here. The conservation status of Alseodaphnopsishokouensis is also re-evaluated according to the IUCN Red List Categories and Criteria in this study.

Re-discovery and taxonomic clarification of Oreocharisleveilleana (Gesneriaceae) in Guizhou, China, over 100 years.

Oreocharisleveilleana Fedde was collected in Ta-pin in 1910 and published in 1911. The collected location was verified within western Luodian County, Guizhou Province, China. However, there have been no records of the species' collection for more than 100 years since then. After extensive investigations by our research team on the type locality and its surrounding areas, we found that it is widely distributed in western Luodian County and eastern Wangmo County, Guizhou Province, China. During further research on the original literature, type specimens and type locality of O.leveilleana, the taxonomic position of O.leveilleana, which was once treated as a synonym of O.auricula (S.Moore) C.B.Clarke, was found to have a taxonomic problem. Through morphological research combined with geographical distribution analysis, it has been determined that it should belong to the genus Petrocodon Hance and it is the same species as P.coccineus (C.Y.Wu ex H.W.Li) Yin Z.Wang. According to the regulations and suggestions of the 2018 "International Code of Nomenclature for Algae, Fungi, and Plants (Shenzhen Code)", we propose and confirm a new combination - Petrocodonleveilleanus (Fedde) X.X.Bai & F.Wen and treat P.coccineus as a synonym of the new combination. Due to its unique bright red flowers within Petrocodon, its original Chinese name has been retained.

Genome-based reclassification of Salipiger manganoxidans (Rajasabapathy et al. 2016) Wirth et al. 2018 as a later heterotypic synonym of Salipiger marinus (Lai et al. 2011) Wirth et al. 2018.

Salipiger manganoxidans VSW210T was compared with Salipiger marinus CK-I3-6T to examine the taxonomic relationship between the two type strains. In phylogenetic trees drawn using whole genome sequences and 16S rRNA gene sequences, S. manganoxidans VSW210T and S. marinus CK-I3-6T clade together and showed a 99.6 % 16S rRNA sequence similarity. The average amino acid identity (AAI), average nucleotide identity (ANIb and ANIm) and digital DNA-DNA hybridization (dDDH) values between S. manganoxidans VSW210T and S. marinus CK-I3-6T were below 97.5, 97.4, 98.4 and 85.1±2.5 %, respectively, all of which were greater than the species delineation threshold AAI value (95.5 %), ANI value (95-96 %) and dDDH value (70 %). Most phenotypic features between both species were almost identical, although there were some differences. The present results show that Salipiger manganoxidans is a later heterotypic synonym of Salipiger marinus.

New insights into the relationship between the average nucleotide identity and the digital DNA-DNA hybridization values in the genus Amycolatopsis and Amycolatopsis cynarae sp. nov., a novel actinobacterium from the rhizosphere soil of Cynara scolymus, and proposal of Amycolatopsis niigatensis as a synonym of Amycolatopsis echigonensis based on comparative genomic analysis.

At present, it is widely believed that a 95-96% average nucleotide identity (ANI) value is equivalent to a 70% digital DNA-DNA hybridization (dDDH) value in the prokaryotic taxonomy. However, in the present study, comparative genome analysis of 29 pairs of Amycolatopsis type strains revealed that a 70% dDDH value did not correspond to a 95-96% ANI based on the MuMmer ultra-rapid aligning tool (ANIm) but approximately corresponded to a 96.6% ANIm value in the genus Amycolatopsis. Based on this corresponding relationship, phenotypic and chemotaxonomical characteristics, as well as phylogenetic analysis, an actinobacterial strain HUAS 11-8T isolated from the rhizosphere soil of Cynara scolymus, was subjected to a polyphasic taxonomic characterization. Based on EzBioCloud alignment, it was found that strain HUAS11-8T had the 16S rRNA gene similarities of 99.78% with A. rhizosphaerae JCM 32589T, 97.8% with A. dongchuanensis YIM 75904T, and < 97.8% sequence similarities to other Amycolatopsis species. Phylogenetic analysis of 16S rRNA gene sequences and whole-genome sequences revealed that strain HUAS 11-8T was closely related to A. rhizosphaerae JCM 32589T. ANIm and dDDH values between strains HUAS 11-8T and A. rhizosphaerae JCM 32589T were 96.3 and 68.5%, respectively, lower than the 96.6 and 70% thresholds recommended for the delineation of a novel Amycolatopsis species. Consequently, strain HUAS 11-8T should represent a novel Amycolatopsis species, for which the name Amycolatopsis cynarae sp. nov. (type strain HUAS 11-8T = MCCC 1K08337T = JCM 35980T) is proposed. Furthermore, based on comparative genomic analysis and rule 42 of the Prokaryotic Code, we propose that Amycolatopsis niigatensis is a later heterotypic synonym of Amycolatopsis echigonensis.

Genome-based classification of the family Haloferacaceae and description of five novel species of Halobaculum.

The taxonomic status of some species of Halobellus, Haloferax, Halogranum, and Haloplanus within the family Haloferacaceae was elucidated by phylogenetic, phylogenomic, and comparative genomic analyses. The relative species of each genus should constitute a single species based on the overall genome-related indexes proposed for species demarcation. The cutoff values of AAI (72.1%), ANI (82.2%), and rpoB' gene similarity (90.7%) were proposed to differentiate genera within the family Haloferacaceae. According to these standards, a novel genus related to the genus Halobaculum was proposed to accommodate Halobaculum halophilum Gai3-2 T and Halobaculum salinum NJ-3-1 T. Five halophilic archaeal strains, DT31T, DT55T, DT92T, SYNS20T, and YSMS11T, isolated from a tidal flat and a marine solar saltern in China, were subjected to polyphasic classification. The phenotypic, phylogenetic, phylogenomic, and comparative genomic analyses revealed that strains DT31T (= CGMCC 1.18923 T = JCM 35417 T), DT55T (= CGMCC 1.19048 T = JCM 36147 T), DT92T (= CGMCC 1.19057 T = JCM 36148 T), SYNS20T (= CGMCC 1.62628 T = JCM 36154 T), and YSMS11T (= CGMCC 1.18927 T = JCM 34912 T) represent five novel species of the genus Halobaculum, for which the names, Halobaculum lipolyticum sp. nov., Halobaculum marinum sp. nov., Halobaculum litoreum sp. nov., Halobaculum halobium sp. nov., and Halobaculum limi sp. nov., are proposed.

The solitary wasp genus Orancistrocerus from Vietnam, with descriptions of two new species (Hymenoptera, Vespidae, Eumeninae).

New data are presented for the potter wasp genus Orancistrocerus van der Vecht (Eumeninae, Odynerini) occurring in Vietnam. Two species are described as new to science: Orancistrocerusthanhnhatsp. nov. and O.thanghensp. nov.Orancistrocerusaterrimuserythropus van der Vecht is synonymized with Orancistrocerusaterrimusaterrimus (de Saussure); the male genitalia of this species are described for the first time. An updated key is presented to all species of the genus.

A review of the semipunctata species group within the genus Lilioceris Reitter, 1913 (Coleoptera, Chrysomelidae).

A new species group of Lilioceris Reitter, 1913 is proposed and reviewed, the semipunctata group. It includes eleven species: L.atrilateralis Kimoto & Takizawa, 1973, L.consentanea (Lacordaire, 1845), L.dentifemoralis Long, 1988, L.discrepens (Baly, 1879), L.jianfenglingensis Long, 1988, L.latissima (Pic, 1932), L.lianzhouensis Long, 2000a, L.rondoni Kimoto & Gressitt, 1979, L.rufometallica (Pic, 1923), L.semipunctata (Fabricius, 1801), and L.yuae Long, 2000b. Liliocerisdiscrepens and L.rondoni were new records from China. Two synonyms are proposed: Liliocerisxinglongensis Long, 1988, syn. nov. of L.consentanea (Lacordaire, 1845), and Criocerisrufimembris Pic, 1921, syn. nov. of L.semipunctata. An identification key, descriptions, habitus photographs, geographic distributions, host plants and habitats (if available), are provided for these species.