Endometrial blood vessel morphometry in patients presenting with abnormal uterine bleeding.

Background: : Abnormal uterine bleeding (AUB) is one of the most common problems encountered in gynecological practice. Various benign and malignant disorders of the endometrial tissue show vascular changes such as congestion, dilatation, and vessel wall irregularities. Aim: To evaluate the vascular morphometry of the endometrial tissue in AUB. Materials and Methods: : A descriptive cross-sectional study of the endometrial tissue in patients presented with AUB was undertaken for vascular morphometric analysis. Histopathological processing of the endometrial tissue samples was done as per the standard format, and the slides were evaluated for vascular morphometry. Results: Out of 150 cases of endometrial tissue in patients presented with AUB, 80 cases were reported as proliferative phase, 41 as secretory phase, 15 as disordered proliferative endometrium, 6 as atrophic phase endometrium, and 4 each of endometrial hyperplasia without atypia and endometrial carcinoma. An average number of endometrial blood vessels and large-sized blood vessels were more in endometrial carcinoma and endometrial hyperplasia without atypia as compared to proliferative phase, secretory phase, atrophic endometrium, and disordered proliferative endometrium. Vessel shape irregularities and vascular congestion were observed in all the cases of atrophic endometrium, endometrial carcinoma, and endometrial hyperplasia without atypia. Endometrial carcinoma showed severe dilatation of the endometrial blood vessels. Conclusion: Vascular morphometry changes were noted in endometrial hyperplasia, endometrial carcinoma, disordered proliferative endometrium, and atrophic phase endometrium. These findings suggest that studies or trials related to anti-angiogenic therapy may help to plan anti-angiogenic therapy in patients with AUB.

Model of Moderate Hyperhomocisteinemia Associated with Mechanical Injury: Dynamics of Morphometric Parameters of Microcirculatory Vessels.

A model of moderate hyperhomocysteinemia associated with mechanical injury of the musculoskeletal system was developed and experimentally substantiated. The adequacy of this model for studies of morphological and functional regularities is verified. This model can be used for the development of a new concept of evaluation of thrombotic complications of mechanical injury.

The correlation of morphological and thrombotic villous arterial lesions with fetal Doppler echocardiographic measurements in the placentas of low-risk term pregnancies.

OBJECTIVE: Few studies have correlated the placental vasculature with fetal cardiac function other than umbilical artery Doppler assessment in low-risk pregnancies. We assessed the contribution of the placental vasculature to fetal echocardiographic parameters using histopathological and morphometric analyses of placental resistance arteries. STUDY DESIGN: Thirty-four low-risk singleton term pregnancies were assessed, including 24 thrombosis-negative cases (no/minimal gross and histological placental abnormalities) and 10 thrombosis-positive cases (histologically identified chorionic plate/stem vessel thrombosis). Fetal ventricular Doppler inflow velocities (E and A waves) and myocardial systolic (S'), early (E'), and late diastolic (A') tissue Doppler velocities were measured within three days before birth. The myocardial performance index (MPI') was calculated. Morphometric variables of placental stem villi arterioles (external diameter 10-150 µm) were examined, including the mean arteriolar density, total cross-sectional lumen area, and wall area/total vessel area (WA/TVA) ratio. RESULTS: The thrombosis-positive group had a higher umbilical artery pulsatility index and a lower tricuspid E'/A' ratio compared to the thrombosis-negative group. The WA/TVA ratio of stem villi arterioles was negatively correlated with tricuspid E, A, and S' velocities as well as the E/E' ratio (n = 34). The tricuspid MPI' was positively correlated with the total cross-sectional lumen area of stem villi arterioles (n = 34). CONCLUSION: We conclude that changes in several fetal echocardiographic parameters are associated with placental vascular histopathological and morphological characteristics in a low-risk population. Further studies are needed to assess whether fetal echocardiographic assessment is a promising prenatal predictor of placental vascular histopathological and morphological characteristics in the general population.

Effect of hyperbaric oxygen therapy on cerebral vasospasm: a vascular morphometric study in an experimental subarachnoid hemorrhage model.

This study was undertaken to investigate the preventive or therapeutic effect of hyperbaric oxygen therapy (HBOT) on cerebral vasospasm following experimental subarachnoid hemorrhage (SAH). Twenty rabbits were assigned randomly to one of four groups. Animals in Group I were not subjected to SAH or sham operation (control group, n = 5). Animals in Group II were subjected to sham operation and received no treatment after the procedure (sham group, n = 5). Animals in Group III were subjected to SAH and received no treatment after SAH induction (SAH group, n = 5). Animals in Group IV were subjected to SAH and received five sessions of HBOT at 2.4 atmospheres absolute (ATA) for 2 h (treatment group, n = 5). Animals were euthanized by perfusion and fixation 72 h after procedures. Basilar artery vasospasm indices, arterial wall thicknesses, and cross-sectional luminal areas were evaluated. Statistical comparisons were performed using Kruskal-Wallis and Mann-Whitney U tests. Mean basilar artery vasospasm index in the treatment group was significantly smaller than in the SAH group. Mean basilar artery wall thickness in the treatment group was significantly smaller than in the SAH group. Mean basilar artery cross-sectional luminal area in the treatment group showed an increase relative to the SAH group, but this difference remained statistically insignificant. Our results demonstrated that repeated application of HBOT at 2.4 ATA for 2 h attenuated vasospastic changes such as increased vasospasm index and arterial wall thickness. HBOT is thus a promising candidate for SAH-induced vasospasm. Further studies are needed to evaluate maximal effect and optimal application regimen.