Assuntos
Antibacterianos/metabolismo , Fusarium/crescimento & desenvolvimento , Fusarium/metabolismo , Ácido Penicílico/análogos & derivados , Ácido Penicílico/metabolismo , Talaromyces/crescimento & desenvolvimento , Talaromyces/metabolismo , Antibacterianos/química , Antibacterianos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , DNA Fúngico/química , DNA Fúngico/genética , Fusarium/classificação , Fusarium/genética , Humanos , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Dados de Sequência Molecular , Estrutura Molecular , Ácido Penicílico/química , Ácido Penicílico/isolamento & purificação , Análise de Sequência de DNA , Talaromyces/classificação , Talaromyces/genéticaRESUMO
In this paper we present the long-term follow-up of two patients, after injection of metallic mercury. Case 1. In 1997, 29-years-old man injected himself to left elbow about 20 ml of metallic mercury by mistake (he was heroin abuser for short time). Mercury concentration in the blood was 400 microg/L. X-ray of the chest, abdomen and affected elbow area showed radiopaque foreign material (depots of mercury). Depots of mercury were also visible on the tricuspid valve in echocardiography. Mercury from the soft tissue left elbow pit was partially surgically removed. During 15 years follow-up two times chelating therapy was performed with d-penicyllamine and DMPS. In 2012, he was admitted to hospital next time. The blood and urine mercury concentration was still elevated (55.2 microg/L and 197 microg/L), mercury depots in the lung and abdomen were present. The signs and symptoms of CNS damage, like peripheral polyneuropathy and ataxia, were diagnosed. CT of brain did not revealed any changes, despite head trauma before 6 years. However neurological findings are typical for chronic mercury poisoning, it is not possible to determine whether these changes are directly related to mercury, because head trauma history, Case 2. In 2003, 16-years-old woman injected herself one month before, in suicidal attempts to both elbows several millilitres of metallic mercury. Mercury concentration in the blood was 56.2 microg/L, in urine 906 microg/L and in the hair 1.12 microg/g. Chest Xray showed depots of mercury in the lung. Mercury from the soft tissue was two times surgically removed. During 9 years two times chelating therapy was performed with d-penicyllamine and DMPS. After 9 years there is no symptoms of mercury poisoning. Mercury depots in the lung are still present. The blood and urine mercury concentration is low (13.7 microg/L and 2.53 microg/L). In mean time she gave birth two healthy children. Further patients evaluation is necessary.
Assuntos
Quelantes/uso terapêutico , Intoxicação por Mercúrio/diagnóstico , Intoxicação por Mercúrio/tratamento farmacológico , Adolescente , Adulto , Feminino , Seguimentos , Humanos , Injeções Intravenosas , Pulmão/química , Masculino , Mercúrio/administração & dosagem , Mercúrio/sangue , Mercúrio/urina , Intoxicação por Mercúrio/sangue , Intoxicação por Mercúrio/urina , Ácido Penicílico/análogos & derivados , Ácido Penicílico/uso terapêutico , Gravidez , Resultado da Gravidez , Tentativa de Suicídio , Resultado do Tratamento , Unitiol/uso terapêutico , Adulto JovemRESUMO
Myasthenia gravis is uncommon in patients with scleroderma, and when diagnosed is usually associated with previous use of d-penicillamine. Clinically, both myasthenia and scleroderma may present with fatigue, weakness and bulbar symptoms, so one of diagnoses may be delayed. We report two new cases and review clinical features of 12 other reported cases of co-existing scleroderma and myasthenia gravis, unrelated to previous d-penicillamine therapy. Co-occurrence of myasthenia and scleroderma was reported almost exclusively (13/14) in women with a mean latency of 7.03 years. Most patients (10/11) had seropositive generalized myasthenia, and there were no cases with exclusively ocular symptoms. Three patients with pre-existing myasthenia were safely treated with d-penicillamine. Myasthenia and scleroderma occur in the context of an underlying autoimmune diathesis, but their co-occurrence could be underreported as the recognition of either disorder may be delayed by overlapping clinical symptoms. Our findings also suggest that d-penicillamine may be cautiously used in selected patients with pre-existing scleroderma and myasthenia, when potential benefits outweigh the risk of possible myasthenia exacerbation.
Assuntos
Miastenia Gravis/diagnóstico , Escleroderma Sistêmico/diagnóstico , Adulto , Anticorpos Antinucleares/sangue , Autoanticorpos/sangue , Comorbidade , Seguimentos , Doença de Hashimoto/diagnóstico , Humanos , Pessoa de Meia-Idade , Miastenia Gravis/cirurgia , Exame Neurológico , Ácido Penicílico/administração & dosagem , Ácido Penicílico/análogos & derivados , Doença de Raynaud/diagnóstico , Escleroderma Sistêmico/tratamento farmacológico , Síndrome de Sjogren/diagnóstico , Timectomia , Hiperplasia do Timo/diagnóstico , Hiperplasia do Timo/cirurgiaRESUMO
In addition to hepatic and extrapyramidal motor clinical symptoms, Wilson's disease patients also exhibit subclinical disorders of other central nervous pathways. In this study, an impairment profile is described by means of eight electrophysiological tests (EAEP, MSEP, TSEP, T-VEP, MEP, EEG, heart frequency variability, and SSR) for 37 patients (28 with neurological, nine with tnon-neurological form) undergoing long-term drug therapy. The occurrence in 64.3% of a delayed wave III and/or IPL III-V prolongation in patients with the neurological form makes pathological FAEP the most common form of the disorder, followed by disorders in MSEP, TSEP, MEP, and T-VEP. Patients with the non-neurological form usually have normal values, although latency prolongations occur in isolated cases. The range of evoked potential findings is characterised primarily by latency prolongations, i.e. a demyelinising impairment type, and significant losses of potential hardly occur (except in the MEP). The electrophysiological impairment profile does not include EEG changes or vegetative disorders.
Assuntos
Doenças dos Gânglios da Base/fisiopatologia , Degeneração Hepatolenticular/fisiopatologia , Doenças do Sistema Nervoso/fisiopatologia , Ácido Penicílico/análogos & derivados , Doenças do Sistema Nervoso Autônomo/diagnóstico , Doenças do Sistema Nervoso Autônomo/tratamento farmacológico , Doenças do Sistema Nervoso Autônomo/fisiopatologia , Doenças dos Gânglios da Base/diagnóstico , Doenças dos Gânglios da Base/tratamento farmacológico , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/fisiopatologia , Quelantes/uso terapêutico , Diagnóstico Diferencial , Estimulação Elétrica , Tratos Extrapiramidais/efeitos dos fármacos , Tratos Extrapiramidais/fisiopatologia , Resposta Galvânica da Pele/efeitos dos fármacos , Resposta Galvânica da Pele/fisiologia , Degeneração Hepatolenticular/diagnóstico , Degeneração Hepatolenticular/tratamento farmacológico , Humanos , Doenças do Sistema Nervoso/diagnóstico , Doenças do Sistema Nervoso/tratamento farmacológico , Exame Neurológico , Ácido Penicílico/uso terapêutico , Nervos Periféricos/efeitos dos fármacos , Nervos Periféricos/fisiopatologia , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/fisiologia , Trientina/uso terapêuticoRESUMO
Thirty patients with Wilson's disease (WD) were observed at a movement disorder clinic between 1970 and 2000. Disease onset was at the mean age (SD) of 14.5 (+/-5.9) years. Presentation with hepatic disease occurred in 12 of 30 patients and with neurologic disease in 15. Three patients were asymptomatic at the time of diagnosis. The mean (SD) delay to diagnosis was 5.9 (+/-5.7) years. Five patients diagnosed in an advanced stage of disease died before initiating treatment. Eighteen patients were followed and treated with D-penicillamine alone or in combination with zinc sulphate. Treatment improved most of neurological symptoms. Dystonic postures, behavioural disturbances and dysarthria were the most resistant neurological signs. 'Pseudo-sclerotic' neurologic involvement predicted a good outcome, whereas hepatic onset and 'classic' neurologic involvement were associated with a poorer prognosis. Two of the 18 treated patients died of hepatic failure due to voluntary discontinuation of therapy. Both D-penicillamine and zinc sulphate were well tolerated. No teratogenic effect of D-penicillamine was observed throughout 5 pregnancies. Our results suggest that D-penicillamine or a combination of D-penicillamine and zinc sulphate is a safe and effective long-term treatment in patients with WD.
Assuntos
Disartria/diagnóstico , Distonia/diagnóstico , Degeneração Hepatolenticular/diagnóstico , Transtornos Mentais/diagnóstico , Ácido Penicílico/análogos & derivados , Adolescente , Adulto , Encéfalo/efeitos dos fármacos , Encéfalo/patologia , Criança , Pré-Escolar , Quimioterapia Combinada , Disartria/tratamento farmacológico , Disartria/mortalidade , Distonia/tratamento farmacológico , Distonia/mortalidade , Feminino , Seguimentos , Degeneração Hepatolenticular/tratamento farmacológico , Degeneração Hepatolenticular/mortalidade , Humanos , Assistência de Longa Duração , Imageamento por Ressonância Magnética , Masculino , Transtornos Mentais/tratamento farmacológico , Transtornos Mentais/mortalidade , Exame Neurológico/efeitos dos fármacos , Ácido Penicílico/efeitos adversos , Ácido Penicílico/uso terapêutico , Gravidez , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento , Sulfato de Zinco/efeitos adversos , Sulfato de Zinco/uso terapêuticoRESUMO
OBJECTIVE: The aim was to elucidate the personality traits of patients with treated Wilsons disease (WD) in comparison to healthy volunteers. METHOD: Twenty-five WD patients, ten females and 15 males, with a mean age of 35.2 +/- 8.3 years completed the Karolinska Scales of Personality (KSP), a self-report inventory comprising 15 separate scales. The results were compared to a control series comprising 200 men and 200 women drawn from the general population. RESULTS: The patients with treated WD scored significantly lower than the healthy controls on aggressivity-hostility-related scales and the scale measuring Psychic Anxiety. Patients with predominantly hepatic symptoms had the lowest aggressivity-related scores and patients with predominantly neurological symptoms had the lowest Irritability, Guilt and Detachment scores and the highest Impulsiveness and Muscular Tension scores. Both groups scored low on the Somatic Anxiety scale. CONCLUSION: The present results illustrate that patients with treated WD have significant deviations in personality traits, especially in aggressivity-hostility-related scales and Psychic Anxiety, compared to healthy controls when investigated by means of a self-report inventory, the KSP. The deviations were not related to age, age at onset or duration of the disease.
Assuntos
Agressão/efeitos dos fármacos , Nível de Alerta/efeitos dos fármacos , Degeneração Hepatolenticular/tratamento farmacológico , Hostilidade , Ácido Penicílico/análogos & derivados , Ácido Penicílico/uso terapêutico , Inventário de Personalidade , Trientina/uso terapêutico , Acetato de Zinco/uso terapêutico , Adulto , Feminino , Seguimentos , Degeneração Hepatolenticular/diagnóstico , Degeneração Hepatolenticular/psicologia , Humanos , Masculino , Pessoa de Meia-Idade , Ácido Penicílico/efeitos adversos , Psicometria , Reprodutibilidade dos Testes , Trientina/efeitos adversos , Acetato de Zinco/efeitos adversosRESUMO
Several thiol-containing molecules (TCM) are currently used as antidotes for nickel, and vicinal TCM seem to be more effective in mobilizing tissue nickel than are mono TCM. Using single cell alkaline electrophoresis, we have shown that the vicinal TCM, meso-2, 3-dimercaptosuccinic acid (DMSA), 2,3-dimercaptopropane-1-sulfonate, and 2,3-dimercaptopropanol markedly enhanced, whereas the mono TCM, D-penicillamide, glutathione, beta-mercaptoethanol, and diethyl dithiocarbomate, reduced nickel chloride (Ni)-induced DNA breaks in a human leukemia cell line, NB4 cells. Ni or TCM alone did not induce plasmid DNA breaks in test tubes and neither did Ni plus mono TCM; however, Ni plus vicinal TCM did. Vicinal TCM did, but mono TCM did not generate H(2)O(2) in solution. H(2)O(2) alone did not, but H(2)O(2) plus Ni induced plasmid DNA breaks. Although Ni plus glutathione did not break DNA, Ni plus glutathione plus H(2)O(2) did. The Ni-DMSA-induced DNA breaks in NB4 cells, as well as in plasmids, were completely prevented by d-mannitol or partially prevented by several antioxidants. Therefore, the DNA breaks induced by Ni plus vicinal TCM seem to be due to the complex of Ni with TCM in concert with the H(2)O(2) produced by the vicinal TCM. The results that DMSA at a concentration as low as 5 microM enhanced the Ni-induced DNA breaks suggest a further evaluation of the TCM as nickel chelators is needed.
Assuntos
Dano ao DNA , Níquel/toxicidade , Compostos de Sulfidrila/farmacologia , Linhagem Celular/efeitos dos fármacos , Ensaio Cometa , Dimercaprol/farmacologia , Ditiocarb/farmacologia , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Glutationa/farmacologia , Humanos , Mercaptoetanol/farmacologia , Níquel/antagonistas & inibidores , Níquel/química , Níquel/farmacologia , Oxirredução , Ácido Penicílico/análogos & derivados , Ácido Penicílico/farmacologia , Plasmídeos/efeitos dos fármacos , Succímero/farmacologia , Compostos de Sulfidrila/químicaRESUMO
The Drosophila DNA-repair test was used in an attempt to detect fungal production of DNA-damaging mycotoxins without an extraction process. 29 species of fungi, 13 Aspergillus, 12 Penicillium and four Fusarium were inoculated directly to a Drosophila medium, and the larvae were then bred in the mouldy medium. Production of DNA-damaging mycotoxin was detected directly by counting the decrease in the survival of DNA-repair-deficient flies. With the direct detection method, Aspergillus ochraceus, A. parasiticus and A. versicolor produced DNA-damaging mycotoxins. The same results were obtained with the mouldy medium extract using the standard DNA-repair test. The direct detection method was convenient for surveying the fungal production of DNA-damaging mycotoxins. The extracts of A. parasiticus and A. versicolor contained aflatoxin B1 and sterigmatocystin, respectively. The DNA-damaging compound in the extract of A. ochraceus was isolated and purified to clear, colourless 'needles'. With nuclear magnetic resonance-mass spectroscopy spectra, the compound was confirmed to be 5,6-dihydropenicillic acid, the DNA-damaging potency of which has not been previously reported.
Assuntos
Dano ao DNA , Reparo do DNA , Drosophila melanogaster/genética , Micotoxinas/análise , Ácido Penicílico/análogos & derivados , Animais , Aspergillus/metabolismo , Meios de Cultura , Feminino , Fusarium/metabolismo , Larva/genética , Masculino , Ácido Penicílico/análise , Penicillium/metabolismo , Espectrofotometria UltravioletaAssuntos
Caproatos/efeitos adversos , Glomerulonefrite/induzido quimicamente , Ácido Penicílico/efeitos adversos , Embolia Pulmonar/diagnóstico por imagem , Veias Renais/diagnóstico por imagem , Adulto , Artrite Reumatoide/tratamento farmacológico , Feminino , Glomerulonefrite/diagnóstico por imagem , Humanos , Ácido Penicílico/análogos & derivados , Ácido Penicílico/uso terapêutico , Embolia Pulmonar/etiologia , Radiografia , Trombose/complicaçõesRESUMO
The authors describe a case of post-traumatic arterio-portal fistula (APF), discovered on the 12th day after coeliomesenteric arteriography performed because of a postoperative complication, and emphasize the rare nature of this lesion, as shown by a review of the published literature. They stress the value of coeliomesenteric arteriography after urgent laparatomy for hepatic trauma for investigation of any possible lesions.
Assuntos
Fístula Arteriovenosa/diagnóstico por imagem , Caproatos/efeitos adversos , Duodeno/irrigação sanguínea , Ácido Penicílico/efeitos adversos , Veia Porta/diagnóstico por imagem , Estômago/irrigação sanguínea , Angiografia , Fístula Arteriovenosa/etiologia , Fístula Arteriovenosa/cirurgia , Humanos , Fígado/lesões , Masculino , Pessoa de Meia-Idade , Ácido Penicílico/análogos & derivados , Ácido Penicílico/uso terapêutico , Ferimentos por Arma de Fogo/complicaçõesRESUMO
The acylase activity was studied with 65 cultures of mycophilic fungi belonging to 56 species and 33 genera. Among these: 9 species displayed the acylase activity toward ampicillin; 8 species, toward phenoxymethylpenicillin; 6 species, toward benzylpenicillin; and 21 species manifested the complex activity. Many of the active species belonged to the bionecrotrophic group of mycophilic fungi, the number of necrotrophic fungi was less, while that of biotrophs and saprotrophs was even lower.