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1.
Regul Toxicol Pharmacol ; 147: 105540, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38070761

RESUMO

Aminocarboxylic acid (ethylenediamine-based) chelating agents such as DTPA are widely used in a variety of products and processes. Recently, DTPA was classified in the European Union as a developmental toxicant CLP Category 1B. However, according to the CLP regulation (CLP, 2008) classification as a developmental toxicant requires a chemical to possess an intrinsic, specific property to do so. This paper provides overwhelming evidence that shows the developmental toxicity only seen at a sustained high dose of 1000 mg DTPA/kg bw/day in rats during pregnancy is mediated by zinc depletion which leads to non-specific secondary effects associated with zinc deficiency. Therefore, based on the CLP regulation itself, viz. the lack of a specific, intrinsic property, supported by significant differences in zinc kinetics and physiology between pregnant rats and pregnant women, DTPA should not be classified as a developmental toxicant. Moreover, classification for developmental toxicity resulting from zinc deficiency, and only observed at high doses, would not increase protection of human health; instead, it will only lead to onerous and disproportionate restrictions being placed on the use of this substance.


Assuntos
Quelantes , Zinco , Feminino , Ratos , Humanos , Gravidez , Animais , Quelantes/toxicidade , Zinco/toxicidade , Ácido Pentético/toxicidade
2.
Toxicol In Vitro ; 70: 105035, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33132172

RESUMO

The epithelial cell plays a key role in the transfer of radionuclides from lungs to blood following pulmonary exposure. The present study was designed to evaluate the transfer across human lung epithelial cells of various actinides (plutonium, americium and uranium), the influence of the physicochemical properties of plutonium compounds and of the chelating agent diethylene triamine pentaacetic acid (DTPA). To address this question, Calu-3 cells grown in a bicameral culture system were used. The integrity of the epithelial barrier was evaluated by measuring transepithelial electrical resistance (TEER) and the passage of a fluorescent marker, lucifer yellow. Activity measurement in basal compartment following periodic collection of culture medium was made from 2 h to seven days. To facilitate data handling and analysis, the statistical tool STATBIODIS was used. The results indicate differences in transfer for the different elements, and according to Pu physicochemical properties. Though to various extents, the chelating agent DTPA always increased the transfer of Pu and Am across the epithelial cells, without altering the integrity of the epithelial barrier. This in vitro cell culture model, by mimicking translocation of actinides from lungs to blood, can represent a valuable tool to further understand the underlying mechanisms and properties controlling this process.


Assuntos
Elementos da Série Actinoide/farmacologia , Quelantes/farmacologia , Células Epiteliais/efeitos dos fármacos , Ácido Pentético/farmacologia , Elementos da Série Actinoide/química , Elementos da Série Actinoide/toxicidade , Transporte Biológico/efeitos dos fármacos , Linhagem Celular Tumoral , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Quelantes/química , Quelantes/toxicidade , Células Epiteliais/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Pulmão/citologia , Ácido Pentético/química , Ácido Pentético/toxicidade
3.
Regul Toxicol Pharmacol ; 97: 197-208, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29964121

RESUMO

Aminocarboxylic acid (ethylenediamine-based) chelating agents, such as DTPA and EDTA, are widely used in a variety of products and processes. Recently the European RAC proposed to classify DTPA as a developmental toxicant Category 1B according to CLP. This paper provides unequivocal and significant evidence that developmental effects cannot be considered an intrinsic property of the chelating substances themselves since: (1) animals fed a zinc deficient diet during gestation exhibit developmental toxicity of a similar nature and severity to that observed in studies involving such chelates, (2) sufficient supplementation of zinc in the diet, or administration of zinc bound chelates, completely negates the developmental effects. Moreover, the bioavailability of DTPA is very low with >95% of oral doses excreted unchanged via the feces within 24 h. If DTPA would possess the intrinsic property to be developmentally toxic, simple zinc supplementation should not be sufficient to negate these effects. Furthermore, the relevance of classification is highly questionable since worker or consumer exposure could not lead to a scenario whereby sufficient zinc deficiency would manifest itself. Therefore classification of DTPA for such effects is not protective of human health; instead it leads to onerous and disproportionate restrictions being placed on this substance.


Assuntos
Quelantes/toxicidade , Ácido Pentético/toxicidade , Animais , Quelantes/administração & dosagem , Humanos , Ácido Pentético/administração & dosagem , Ácido Pentético/antagonistas & inibidores , Zinco/farmacologia
4.
Environ Sci Technol ; 47(17): 9942-9, 2013 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-23888885

RESUMO

Gadolinium chelates are used in increasing amounts as contrast agents in magnetic resonance imaging, and their fate in wastewater treatment has recently become the focus of research. Oxidative processes, in particular the application of ozone, are currently discussed or even implemented for advanced wastewater treatment. However, reactions of the gadolinium chelates with ozone are not yet characterized. In this study, therefore, rate constants with ozone were determined for the three commonly used chelates Gd-DTPA, Gd-DTPA-BMA, and Gd-BT-DO3A, which were found to be 4.8 ± 0.88, 46 ± 2.5, and 24 ± 1.5 M(-1) s(-1), respectively. These low rate constants indicate that a direct reaction with ozone in wastewater is negligible. However, application of ozone in wastewater leads to substantial yields of (•)OH. Different methods have been applied and compared for determination of k((•)OH+Gd chelate). From rate constants determined by pulse radiolysis experiments (k((•)OH+Gd-DTPA) = 2.6 ± 0.2 × 10(9) M(-1) s(-1), k((•)OH+Gd-DTPA-BMA) = 1.9 ± 0.7 × 10(9) M(-1) s(-1), k((•)OH+Gd-BT-DO3A) = 4.3 ± 0.2 × 10(9) M(-1) s(-1)), it is concluded that a reaction in wastewater via (•)OH radicals is feasible. Toxicity has been tested for educt and product mixtures of both reactions. Cytotoxicity (MTT test) and genotoxicity (micronuclei assay) were not detectable.


Assuntos
Quelantes/química , Meios de Contraste/química , Gadolínio/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/química , Animais , Células CHO , Quelantes/toxicidade , Cricetulus , Ácido Edético/química , Ácido Edético/toxicidade , Radical Hidroxila/química , Imageamento por Ressonância Magnética , Oxirredução , Ozônio/química , Ozônio/toxicidade , Ácido Pentético/química , Ácido Pentético/toxicidade , Poluentes Químicos da Água/toxicidade
5.
Environ Health Perspect ; 118(12): 1728-34, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20663738

RESUMO

BACKGROUND: Proximity to traffic-related pollution has been associated with poor respiratory health in adults and children. OBJECTIVES: We wished to test the hypothesis that particulate matter (PM) from high-traffic sites would display an enhanced capacity to elicit inflammation. METHODS: We examined the inflammatory potential of coarse [2.5-10 µm in aerodynamic diameter (PM(2.5-10))] and fine [0.1-2.5 µm in aerodynamic diameter (PM(0.1-2.5))] PM collected from nine sites throughout Europe with contrasting traffic contributions. We incubated murine monocytic-macrophagic RAW264.7 cells with PM samples from these sites (20 or 60 µg/cm²) and quantified their capacity to stimulate the release of arachidonic acid (AA) or the production of interleukin-6 and tumor necrosis factor-α (TNFα) as measures of their inflammatory potential. Responses were then related to PM composition: metals, hydrocarbons, anions/cations, and endotoxin content. RESULTS: Inflammatory responses to ambient PM varied markedly on an equal mass basis, with PM(2.5-10) displaying the largest signals and contrasts among sites. Notably, we found no evidence of enhanced inflammatory potential at high-traffic sites and observed some of the largest responses at sites distant from traffic. Correlation analyses indicated that much of the sample-to-sample contrast in the proinflammatory response was related to the content of endotoxin and transition metals (especially iron and copper) in PM(2.5-10). Use of the metal chelator diethylene triamine pentaacetic acid inhibited AA release, whereas recombinant endotoxin-neutralizing protein partially inhibited TNFα production, demonstrating that different PM components triggered inflammatory responses through separate pathways. CONCLUSIONS: We found no evidence that PM collected from sites in close proximity to traffic sources displayed enhanced proinflammatory activity in RAW264.7 cells.


Assuntos
Poluentes Atmosféricos/toxicidade , Macrófagos/efeitos dos fármacos , Material Particulado/toxicidade , Animais , Ácido Araquidônico/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Mediadores da Inflamação/metabolismo , Camundongos , Tamanho da Partícula , Ácido Pentético/toxicidade , Fator de Necrose Tumoral alfa/metabolismo
6.
Int J Toxicol ; 27 Suppl 2: 71-92, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18830865

RESUMO

Pentasodium Pentetate and Pentetic Acid function as chelating agents in cosmetics. Pentasodium Pentetate is readily soluble in water, but the corresponding free acid is not. Pentasodium Pentetate is used in almost 400 cosmetic products over a wide range of product categories, although it is mostly used in hair dyes and colors at use concentrations of 0.1% to 1.0%. Pentetic Acid is used in 150 cosmetic products, mostly in hair dyes and colors. Chelating agents are used in cosmetics to remove calcium and magnesium cations, which impede foaming and cleansing performance and which can cause a haze in clear liquids. The acute oral LD(50) of Pentasodium Pentetate in rats was > 5 g/kg. The acute dermal LD(50) of Pentapotassium Pentetate using rats was reported to be > 2 g/kg. The intraperitonal LD(50) of Pentetic Acid was reported to be 585 mg/kg. Short-term studies of the calcium and sodium salts of Pentetic Acid in male mice demonstrated no dose-related toxicity over the dose range of 10, 100, and 250 mg/kg. In a 4-week dermal toxicity study, daily topical application of 0.05% Pentasodium Pentetate to shaved and abraded rabbit skin produced moderate erythema after the first week and throughout the study, but no systemic toxicity. Pentasodium Pentetate or Pentapotassium Pentetate applied to intact albino rabbit skin were not irritating. A 40% solution of Pentapotassium Pentetate was not sensitizing in a guinea pig maximization test. The no observed adverse effect level (NOAEL) for rats given 40% Pentapotassium Pentetate by oral gavage was reported to be 83 mg/kg day(-1). Subchronic inhalation evaluation of a bath freshener containing 0.05% or 0.09% Pentasodium Pentetate using albino rats determined that there was no cumulative systemic toxicity attributable to the ingredient at either concentration. The no observed effect level (NOEL) for maternal toxicity in pregnant rats was 400 mg/kg body weight and for fetal toxicity was 100 mg/kg body weight. Another reproductive toxicity study evaluated Pentetic Acid-Zn with and without sodium chloride in pregnant C57/B1 Dougherty mice. No toxicity was found without added sodium chloride. Pentapotassium Pentetate was not mutagenic in an Ames test, with or without metabolic activation. The same material tested in Chinese hamster ovary cells was not clastogenic. Calcium Pentetate at 1.351 microg/ml produced a statistically significant increase in the number of sister-chromatid exchanges. Pentasodium Pentetate is nonirritating to moderately irritating, but not a sensitizer in clinical tests. A human comedogenicity (acne promotion) test using Pentasodium Pentetate found no effect. Although data are lacking on the dermal penetration of these two ingredients, other chelating agents such as EDTA do not penetrate the skin, so it is likely that Pentasodium Pentetate and Pentetic Acid also would not penetrate. The high water solubility of Pentasodium Pentetate and the low water solubility of Pentetic Acid also support that their dermal penetration will be low. Other chelating agents, including EDTA and its salts, have been determined to be safe in the current practices of use in cosmetics. Meta-, Tri-, and Hexametaphosphate salts are chelating agents determined to be safe in the current practices of use in cosmetics. Metasilicate salts were found to be safe as chelating agents in cosmetics when formulated to avoid irritation. Overall, these data were considered sufficient to support the safety of Pentesodium Pentetate and Pentetic Acid as used in cosmetics.


Assuntos
Cosméticos/química , Ácido Pentético/toxicidade , Animais , Feminino , Humanos , Masculino , Estrutura Molecular , Testes de Mutagenicidade , Ácido Pentético/química , Pele/efeitos dos fármacos , Teratogênicos/química , Teratogênicos/toxicidade
7.
Eksp Klin Farmakol ; 71(4): 41-6; discussion 47-8, 2008.
Artigo em Russo | MEDLINE | ID: mdl-18819440

RESUMO

The preclinical evaluation of 0.5 M solution of a manganese(II)-DTPA complex (Mangapentetate, Pentamang) has been carried out in order to test the ability of manganese to be used as substitute for potentially toxic gadolinium in paramagnetic contrast agents in the MRI clinical routines. The toxicologic tests of the Mn(II) - DTPA were carried out in mice, rats, and rabbits. Liquid phantoms served for direct comparison of the ability of Mn(II) - DTPA to increase the intensity of T1-weighted SE-images to the contrast properties of the Gd(III) - DTPA (Magnevist). Normal healthy rabbits (n = 12) were used for quantification of the imaging ability of Mn(II) - DTPA. The value of LD50 in rabbits was above 10 ml/kg, rather close to that one of Gd(III) - DTPA. An increase in intensity of the T1-weighted images induced by addition of Mn(II) - DTPA in phantom tests did not differ significantly from the values obtained with Gadopentetate. Mn(II) - DTPA delivered prominent enhancement of normal kidneys in healthy rabbits as well as chest tumors in dogs.


Assuntos
Meios de Contraste , Ácido Pentético , Animais , Meios de Contraste/toxicidade , Feminino , Dose Letal Mediana , Imageamento por Ressonância Magnética , Masculino , Camundongos , Ácido Pentético/toxicidade , Ratos
8.
Eksp Klin Farmakol ; 71(4): 49-52, 2008.
Artigo em Russo | MEDLINE | ID: mdl-18819441

RESUMO

We have carried out a preclinical toxicological investigation (acute toxicity evaluation) of Mangascan (0.5 M solution of manganese(II) - EDTA complex) and Pentamang (0.5 M solution of manganese(II) - DTPA complex), a new paramagnetic contrast agents for MRI procedures. In 14 days after single intravenous introduction of Mangascan (10.0 ml/kg) or Pentamang (5.0 ml/kg) to rats, no any toxic influence of the studied agents was detected in the general condition, bone marrow, cardiovascular and central nervous systems, and liver and kidney functions of experimental animals. No pathological changes were observed in the functional activity and morphology of the internal organs and systems. The results showed that both Mangascan and Pentamang belong to the class of low toxicity substances.


Assuntos
Meios de Contraste/toxicidade , Ácido Edético/toxicidade , Ácido Pentético/toxicidade , Animais , Feminino , Imageamento por Ressonância Magnética , Masculino , Camundongos , Ratos
9.
Mediators Inflamm ; 2005(5): 280-92, 2005 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-16258195

RESUMO

S100A8/A9 (calprotectin), which is released by neutrophils under inflammatory conditions, has the capacity to induce apoptosis in various cells. We previously reported that S100A8/A9 induces apoptosis of EL-4 lymphoma cells via the uptake of extracellular zinc in a manner similar to DTPA, a membrane-impermeable zinc chelator. In this study, S100A8/A9-induced apoptosis was examined in several cell lines that are weakly sensitive to DTPA, suggesting S100A8/A9 is directly responsible for apoptosis in these cells. Since zinc inhibits apoptosis of MM46, one of these cells, the regulation by zinc of the capacity of S100A8/A9 to bind MM46 cells was studied. When MM46 cells were incubated with S100A8/A9 in standard or zinc-depleted medium, the amounts of S100A8/A9 bound to cells was markedly lower at 3 h than at 1 h. In contrast, when MM46 cells were incubated with S100A8/A9 in the presence of high levels of zinc, binding to cells was the same at 1 and 3 h. When the cells were permeabilized with saponin prior to analysis, a larger amount of cell-associated S100A8/A9 was detected at 3 h. The amount was further increased in cells treated with chloroquine, suggesting that S100A8/A9 was internalized and degraded in lysosomes. Although it has been reported that S100A8/A9 binds to heparan sulfate on cell membranes, the amount of S100A8/A9 bound to MM46 cells was not reduced by heparinase treatment, but was reduced by trypsin treatment. These results suggest that S100A8/A9 induces apoptosis by direct binding to MM46 cells, and that this activity is regulated by zinc.


Assuntos
Apoptose/fisiologia , Complexo Antígeno L1 Leucocitário/metabolismo , Zinco/farmacologia , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Quelantes/toxicidade , Heparitina Sulfato/farmacologia , Humanos , Iontoforese , Complexo Antígeno L1 Leucocitário/farmacologia , Complexo Antígeno L1 Leucocitário/toxicidade , Camundongos , Ácido Pentético/toxicidade , Ligação Proteica/efeitos dos fármacos
10.
Environ Pollut ; 133(2): 199-211, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15519451

RESUMO

Potential contamination at ex-industrial sites means that, prior to change of use, it will be necessary to quantify the extent of risks to potential receptors. To assess ecological hazards, it is often suggested to use biological assessment to augment chemical analyses. Here we investigate the potential of a commonly recommended bioassay, the earthworm reproduction test, to assess the status of urban contaminated soils. Sample points at all study sites had contaminant concentrations above the Dutch soil criteria Target Values. In some cases, the relevant Intervention Values were exceeded. Earthworm survival at most points was high, but reproduction differed significantly in soil from separate patches on the same site. When the interrelationships between soil parameters and reproduction were studied, it was not possible to create a good model of site soil toxicity based on single or even multiple chemical measurements of the soils. We thus conclude that chemical analysis alone is not sufficient to characterize soil quality and confirms the value of biological assays for risk assessment of potentially contaminated soils.


Assuntos
Oligoquetos/efeitos dos fármacos , Poluentes do Solo/análise , Animais , Bioensaio/métodos , Cálcio/análise , Cálcio/toxicidade , Minas de Carvão , Ecossistema , Exposição Ambiental/efeitos adversos , Resíduos Industriais/efeitos adversos , Magnésio/análise , Magnésio/toxicidade , Metais Pesados/análise , Metais Pesados/toxicidade , Oligoquetos/crescimento & desenvolvimento , Ácido Pentético/análise , Ácido Pentético/toxicidade , Potássio/análise , Potássio/toxicidade , Reprodução , Poluentes do Solo/toxicidade
11.
Radiats Biol Radioecol ; 44(2): 221-8, 2004.
Artigo em Russo | MEDLINE | ID: mdl-15174385

RESUMO

An effect of cincacine at three doses (25, 150 and 300 mumol/kg) has been studied in rats receiving 241Am citrate intragastrically. The radionuclide was introduced every other day for 2 weeks. The total content was 925 kBq/kg. A cincacine administration leads to limitation of radionuclide accumulation in the major organs of deposition independent of the modes of intake. At gastrointestinal 241Am intake peroral cincacine administration is more effective in limiting this radionuclide accumulation in skeleton but less effective in reduction of its accumulation in liver compared to parenteral cincacine. No reliable dependence of cincacine efficacy on dosage has been revealed. A morphology study of organs has shown that cincacine ingestion at a dose of 150 mumol/kg for 4 weeks and at a dose of 300 mumol/kg for 2 weeks produces a toxic effect on the small intestine mucosa. 25 mumol/kg is the optimum dose and per os administration of higher doses is not expedient.


Assuntos
Amerício/química , Quelantes/farmacologia , Ácido Pentético/farmacologia , Administração Oral , Amerício/metabolismo , Animais , Osso e Ossos/química , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Quelantes/administração & dosagem , Relação Dose-Resposta a Droga , Injeções Intraperitoneais , Mucosa Intestinal/química , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Masculino , Ácido Pentético/administração & dosagem , Ácido Pentético/toxicidade , Ratos , Vísceras/química , Vísceras/efeitos dos fármacos , Vísceras/metabolismo
12.
Nucl Med Biol ; 30(5): 457-64, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12831982

RESUMO

We report the preclinical testing of a synthetic receptor-binding macromolecule, [(99m)Tc]DTPA-mannosyl-dextran (36 kDa, 8 DTPA and 55 mannosyl units per dextran, K(D) = 0.12 nM), for sentinel node detection. Nonclinical safety studies included cardiac pharmacology safety studies, acute toxicology and pathology studies at 50 and 500 times the scaled human dose in both rats and rabbits after foot pad administration, and perivascular irritation studies in rabbits following intra-muscular administration at 100 and 1000 times the scaled human dose. Biodistribution studies in rabbits at 15 m, 1 h, and 3 h indicated that [(99m)Tc]DTPA-mannosyl-dextran cleared the hind foot pad with a biological half-life of 2.21 +/- 0.27 h. Other than mild hepatocyte hypertrophy in rabbits, no abnormalities in toxicology or pathology were found. Intravenous administration had no effect on survival, any clinical observations, electrocardiograms, or blood pressures. Intramuscular injection had no effect on survival, clinical observations, injection site observations, or injection site histopathology. The estimated absorbed radiation dose to the affected breast was 0.15 mGy/MBq and the effective dose was 1.06 x 10(-2) mSv/MBq. This preclinical study demonstrates that [(99m)Tc]DTPA-mannosyl-dextran has no toxicities and has an acceptable biodistribution and radiation dose.


Assuntos
Dextranos/efeitos adversos , Dextranos/farmacocinética , Linfonodos/metabolismo , Mananas/efeitos adversos , Mananas/farmacocinética , Compostos de Organotecnécio/efeitos adversos , Compostos de Organotecnécio/farmacocinética , Ácido Pentético/efeitos adversos , Ácido Pentético/farmacocinética , Radiometria/métodos , Animais , Carga Corporal (Radioterapia) , Doenças Cardiovasculares/etiologia , Dextranos/toxicidade , Cães , Avaliação Pré-Clínica de Medicamentos , Estabilidade de Medicamentos , Feminino , Humanos , Linfonodos/diagnóstico por imagem , Contagem de Linfócitos , Masculino , Mananas/toxicidade , Modelos Biológicos , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Especificidade de Órgãos , Compostos de Organotecnécio/toxicidade , Ácido Pentético/toxicidade , Coelhos , Doses de Radiação , Cintilografia , Compostos Radiofarmacêuticos/efeitos adversos , Compostos Radiofarmacêuticos/farmacocinética , Compostos Radiofarmacêuticos/toxicidade , Ratos , Análise de Sobrevida , Pentetato de Tecnécio Tc 99m/análogos & derivados , Distribuição Tecidual
13.
Breast Cancer Res Treat ; 71(2): 149-59, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11881911

RESUMO

Metastasis is the principal cause of death in breast cancer patients. New and improved treatments for eradicating micrometastases are needed. To this end, a novel alpha-emitting protein construct, 213Bi-labelled plasminogen activator inhibitor type-2 (PAI-2) (alpha-PAI-2), was evaluated in vitro. This construct exploits: (a) the overexpression of the cell-surface receptor bound urokinase plasminogen activator (uPA) in the metastatic spread of breast cancer cells; (b) the binding and inhibition of receptor-bound uPA by PAI-2; and (c) the high cytotoxicity of alpha radiation. High labeling efficiencies and stability of 213Bi bound to human recombinant PAI-2 conjugated with cyclic diethylenetriaminepentaacetic acid anhydride were achieved (greater than 90%). The uPA inhibitory activity of the chelated PAI-2 was maintained as determined by complex formation with uPA and by inhibition of uPA activity. Furthermore, the reactivity of alpha-PAI-2 was confirmed in a cell assay as this construct was highly cytotoxic to breast cancer cell lines that express active, receptor bound uPA. The specificity of alpha-PAI-2 targeting was shown using several controls. Firstly, an active uPA blocking agent that limits PAI-2 binding significantly improved cell survival by a factor greater than three. Secondly, a non-specific alpha-BSA construct had minimal cytotoxic effect. Moreover, alpha-PAI-2 was not cytotoxic to freshly isolated normal human leukocytes, confirming that cells which do not contain active, receptor bound uPA cannot be targeted by alpha-PAI-2. In conclusion, we have validated, in vitro, the potential of alpha-PAI-2 as a novel therapeutic agent for breast cancer.


Assuntos
Bismuto/toxicidade , Sobrevivência Celular/efeitos da radiação , Inibidor 2 de Ativador de Plasminogênio/toxicidade , Neoplasias da Mama , Sobrevivência Celular/efeitos dos fármacos , Feminino , Humanos , Cinética , Metástase Neoplásica , Ácido Pentético/toxicidade , Radioisótopos , Proteínas Recombinantes/toxicidade , Células Tumorais Cultivadas
14.
J Asian Nat Prod Res ; 3(1): 63-75, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11355772

RESUMO

Salvia miltiorrhiza is a traditional Chinese medicine which has been well documented for its anti-cancer effects. Based on the structure of danshinone, one of the active compounds derived from Salvia miltiorrhiza, we synthesized a simplified phenolic analog, S-3-1, and tried to explore its possible actions in preventing the development of cancer. With the Ames test, S-3-1 was found to efficiently suppress the mutagenicity of benzo[alpha]pyrene. This result is consistent with the inhibitory effect of S-3-1 on the activation of benzo[alpha]pyrene by hepatic microsomal enzymes. Besides the anti-initiation effects, S-3-1 could significantly inhibit the croton oil-induced increase of mouse skin epithermal ornithine decarboxylase activity. Moreover, S-3-1 quenched both superoxide and hydroxyl free radicals whereas it inhibited lipid peroxidation in the in vitro model. These results suggest that S-3-1 might act as anti-initiation and anti-promotion agents through reversing the biochemical alterations induced by carcinogen during carcinogenesis. Therefore, we further investigated the effects of S-3-1 on carcinogenesis. In vitro, S-3-1 inhibited the benzo[alpha]pyrene-induced transformation of V79 Chinese hamster lung fibroblasts. At 10-40 mg/kg, S-3-1 was found to inhibit the development of DMBA/croton oil-induced skin papilloma in mice through decreasing the incidence of papilloma, prolonging the latent period of tumor occurrence and reducing tumor number per mouse in a dose-dependent manner. We concluded from this study that S-3-1 might be developed as a new chemopreventive drug.


Assuntos
Anticarcinógenos/isolamento & purificação , Anticarcinógenos/farmacologia , Benzofuranos/isolamento & purificação , Benzofuranos/farmacologia , Bepridil/análogos & derivados , Medicamentos de Ervas Chinesas/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/farmacologia , Papiloma/induzido quimicamente , Fenantrenos/isolamento & purificação , Fenantrenos/farmacologia , Picratos , Plantas Medicinais/química , Neoplasias Cutâneas/induzido quimicamente , 9,10-Dimetil-1,2-benzantraceno , Animais , Anticarcinógenos/química , Anticarcinógenos/farmacocinética , Benzo(a)pireno , Benzofuranos/química , Benzofuranos/farmacocinética , Bepridil/toxicidade , Compostos de Bifenilo , Transformação Celular Neoplásica/induzido quimicamente , Células Cultivadas/efeitos dos fármacos , Cricetinae , Óleo de Cróton , Cisteína/metabolismo , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Células Epiteliais/enzimologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/farmacocinética , Hipoxantina/toxicidade , Técnicas In Vitro , Ferro/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Masculino , Medicina Tradicional Chinesa , Camundongos , Camundongos Endogâmicos ICR , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/enzimologia , Microssomos Hepáticos/metabolismo , Estrutura Molecular , Mutagênicos , Ornitina/metabolismo , Ornitina Descarboxilase/metabolismo , Ácido Pentético/toxicidade , Fenantrenos/química , Fenantrenos/farmacocinética , Ratos , Salmonella/efeitos dos fármacos , Pele/efeitos dos fármacos , Pele/enzimologia , Pele/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Relação Estrutura-Atividade , Xantina Oxidase/metabolismo
15.
Invest Radiol ; 35(6): 373-9, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10853612

RESUMO

RATIONALE AND OBJECTIVES: In vivo studies have shown species-specific toxicity after application of the liver-specific contrast agent Dy-ethoxybenzyl (EOB)-DTPA. To predict species differences in the laboratory, an in vitro model of the liver was used to examine the divergent results. METHODS: Rat, canine, porcine, and human hepatocytes were isolated and embedded between layers of collagen. During and after 48 hours of incubation with different concentrations of Dy-EOB-DTPA (maximum concentration 50 mmol/L), morphological changes and enzyme leakage were determined. RESULTS: The response to the contrast agent varied for hepatocytes from different species. For canine cells, morphological changes and cell death were evident with as little as 5 mmol/L Dy-EOB-DTPA. Rat hepatocytes tolerated up to 50 mmol/L Dy-EOB-DTPA, and enzyme leakage was transient. Only after incubation with 50 mmol/L Dy-EOB-DTPA was the formation of intracellular vacuoles evident. In contrast, even the highest concentration of Dy-EOB-DTPA did not cause an enzyme leakage of porcine or human hepatocytes, although similar vacuoles were seen. CONCLUSIONS: These data demonstrate a species-dependent toxicity for Dy-EOB-DTPA in vitro, with similar responses in porcine and human hepatocytes.


Assuntos
Meios de Contraste/toxicidade , Fígado/efeitos dos fármacos , Ácido Pentético/análogos & derivados , Ácido Pentético/toxicidade , Animais , Células Cultivadas/efeitos dos fármacos , Cães , Feminino , Humanos , Fígado/citologia , Masculino , Ratos , Ratos Endogâmicos Lew , Ratos Wistar , Suínos , Testes de Toxicidade
16.
Nucl Med Biol ; 26(5): 581-9, 1999 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10473198

RESUMO

The biodistribution and tissue toxicity of intravenously administered 225-actinium (225Ac) complexed with acetate, ethylene diamine tetraacetic acid (EDTA), 1, 4, 7, 10, 13-pentaazacyclopentadecane-N, N', N", N"', N""-pentaacetic acid (PEPA), or the "a" isomer of cyclohexyl diethylenetriamine pentaacetic acid (CHX-DTPA), were examined. The percent of injected dose per organ and per gram of tissue for each chelate complex was determined. 225Ac-CHX-DTPA was evaluated further for radiotoxic effects. Mice receiving > or =185 kBq 225Ac-CHX-DTPA suffered 100% morbidity by 5 days and 100% mortality by 8 days postinjection, and all animals evaluated had significant organ damage. The in vivo instability of the 225Ac-CHX-DTPA complex likely allowed accumulation of free 225Ac in organs, which resulted in tissue pathology.


Assuntos
Actínio/farmacocinética , Quelantes/farmacocinética , Isotiocianatos/farmacocinética , Ácido Pentético/análogos & derivados , Actínio/toxicidade , Animais , Quelantes/toxicidade , Relação Dose-Resposta à Radiação , Feminino , Isotiocianatos/síntese química , Isotiocianatos/toxicidade , Camundongos , Camundongos Endogâmicos BALB C , Ácido Pentético/síntese química , Ácido Pentético/farmacocinética , Ácido Pentético/toxicidade , Relação Estrutura-Atividade , Distribuição Tecidual
17.
Ecotoxicol Environ Saf ; 43(1): 74-82, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10330324

RESUMO

The chronic effects of the chelating agent diethylenetriamine pentaacetic acid (DTPA) on reproduction, condition factor, liver somatic index (LSI), gonad somatic index (GSI), and ethoxyresorufin O-deethylase (EROD) activity of adult Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) were assessed. Breeding groups of three females and two males were exposed to 0, 1, 10, or 100 mg/liter DTPA (nominal) in a 28-day "static-renewal" experiment. Overall, the toxicity of DTPA to adult crimson-spotted rainbowfish was relatively low. Reproduction was not affected at concentrations up to 100 mg/liter DTPA, although an early effect on hatchability was potentially attributed to direct toxicity to rainbowfish eggs. DTPA also had little effect on the condition of adult rainbowfish, with condition factor and GSI being unaffected at concentrations up to 100 mg/liter, the latter finding supporting the reproduction results. However, LSI in male rainbowfish exposed to 100 mg/liter was significantly lower than in those exposed to 1 mg/liter DTPA (P

Assuntos
Quelantes/toxicidade , Citocromo P-450 CYP1A1/metabolismo , Peixes/fisiologia , Ácido Pentético/toxicidade , Reprodução/efeitos dos fármacos , Animais , Feminino , Peixes/metabolismo , Gônadas/anatomia & histologia , Gônadas/efeitos dos fármacos , Fígado/anatomia & histologia , Fígado/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Óvulo/efeitos dos fármacos , Fatores de Tempo
18.
J Nucl Med ; 40(1): 166-76, 1999 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9935073

RESUMO

UNLABELLED: The alpha-particle-emitting radionuclides have several physical characteristics that make them attractive candidates for radioimmunotherapy: (a) high linear energy transfer; (b) short path lengths (50-80 microm); and (c) limited ability of cells to repair damage to DNA. This article describes the pharmacokinetic, bioactivity, toxicity and chemical characteristics of alpha-particle-emitting, 213Bi and 212Bi radiometal conjugated HuM195 (anti-CD33) constructs. Conjugation of HuM195 to SCN-CHX-A-DTPA resulted in the attachment of up to 10 chelating ligand molecules per antibody. RESULTS: Radiolabeling efficiency of the CHX-A-DTPA-HuM195 construct with 213Bi was 78%+/-10% (n = 46) after 10 min at specific activities of up to 1110 MBq/mg. The immunoreactivity of the 213Bi-labeled CHX-A-DTPA-HuM195 construct was 84%+/-10% (n = 28) and was independent of the specific activity. The bismuth-labeled CHX-A-DTPA-HuM195 construct was rapidly internalized into the cell in a time-dependent manner ranging from 50% at 1 h to 65% at 24 h. 205Bi/206Bi-labeled constructs were stable for at least 2 d in vitro in the presence of human serum at 37 degrees C. After injection into mice, there was no uptake or loss of bismuth to mouse tissues, which do not express CD33, or to the kidney, which has avidity for free bismuth. Mice injected intraperitoneally with doses of (213Bi)CHX-A-DTPA-HuM1 95 ranging from 18.5 to 740 MBq/kg showed no toxicity, but at 2590 MBq/kg, two of the three mice died within 2 wk and a third mouse showed significant reductions in white blood cell counts. Mice injected intravenously with doses of (213Bi)CHX-A-DTPA-HuM195 up to 370 MBq/kg exhibited little toxicity, but 666 MBq/kg was above the MTD for mice. Leukemia cell killing in vitro with bismuth-labeled HuM1 95 showed dose- and specific activity-dependent killing of CD33+ HL60 cells; approximately 50% killing was observed when two bismuth atoms (50 fM radiolabeled antibody) were initially bound onto the target cell surface. CONCLUSION: Alpha-emitting antibodies are among the most potent cytotoxic agents known, yet are specific and appear safe in vivo. The physical and biochemical characteristics of the 213Bi isotope and its generation, as well as the biochemistry of the 213Bi-labeled CHX-A-DTPA-HuM195 construct, make it possible to use the constructs safely and feasibly in humans at therapeutic levels.


Assuntos
Anticorpos Monoclonais , Antígenos CD/imunologia , Antígenos de Diferenciação Mielomonocítica/imunologia , Ácido Pentético/análogos & derivados , Partículas alfa , Animais , Células HL-60/patologia , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Ácido Pentético/química , Ácido Pentético/imunologia , Ácido Pentético/farmacocinética , Ácido Pentético/toxicidade , Radioimunoterapia , Proteínas Recombinantes , Lectina 3 Semelhante a Ig de Ligação ao Ácido Siálico
19.
Int J Radiat Biol ; 75(8): 929-41, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10465359

RESUMO

PURPOSE: To reduce the long-term toxicity of 239Pu in rats by lifetime drinking of ZnDTPA solution and to investigate possible side-effects of the drug. MATERIALS AND METHODS: Male Sprague-Dawley rats received a single injection of 239Pu citrate, alone or plus oral ZnDTPA. Additional groups were administered only ZnDTPA. Late tissue changes were evaluated by post-mortem examination, X-rays and histologically. RESULTS: The incidence of rat bearing osteosarcoma decreased after treatment to 35% as compared with 53% in untreated controls. The proportional incidence of osteosarcomas was reduced after ZnDTPA by more than the corresponding removal of 239Pu. Unexpectedly in the male rat, mammary tumours, mostly malignant, developed in 20% of rats that received 239Pu as compared with 0.5% in the untreated controls. After a lifetime drinking solely 3 x 10(-3) M ZnDTPA the incidence of diffuse glomerulosclerosis reached 29% as compared with 10% in controls. CONCLUSIONS: In rat, protracted oral administration of ZnDTPA reduced the incidence of osteosarcomas after injection of 239Pu, even if treatment started with a delay of 1 month. In the latter case, however, more soft tissue damage was found than after treatment beginning at 4 days post-239Pu. An increased incidence of diffuse glomerulosclerosis was observed as a side effect of oral ZnDTPA only when given continuously, alone and in high amounts.


Assuntos
Ácido Pentético/farmacologia , Plutônio/toxicidade , Administração Oral , Animais , Neoplasias Ósseas/prevenção & controle , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/efeitos da radiação , Glomerulosclerose Segmentar e Focal/induzido quimicamente , Masculino , Neoplasias Mamárias Experimentais/prevenção & controle , Osteossarcoma/prevenção & controle , Ácido Pentético/toxicidade , Plutônio/farmacocinética , Radiometria , Ratos , Ratos Sprague-Dawley , Taxa de Sobrevida , Fatores de Tempo , Distribuição Tecidual/efeitos dos fármacos
20.
J Nutr ; 128(10): 1600-5, 1998 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9772124

RESUMO

Growth failure in zinc-deficient animals is associated with decreased DNA synthesis; zinc deprivation of 3T3 cells, by use of diethylenetrinitrilopentaacetate (DTPA), impairs thymidine incorporation when the cells are stimulated with fetal bovine serum (FBS). The purpose of this study was to determine the step of cell cycle progression that is affected by zinc deprivation. Swiss murine 3T3 cells were cultured for 3 d in complete media and then for 2 d in low serum media. Cells were then placed in serum-free media and stimulated in sequence with platelet-derived growth factor (PDGF; 3 h), epidermal growth factor (EGF; 0.5 h) and insulin-like growth factor-I (IGF-I; 16 h). The combination of growth factors stimulated thymidine incorporation to the same extent as 10% FBS, and DTPA or EDTA (0.6 mmol/L) inhibited thymidine incorporation. Inhibition was prevented by addition of zinc, but not calcium, iron or cadmium (0.4 mmol/L). When DTPA was present during all stages with no addition of zinc, or zinc added during the competency-priming (PDGF and EGF) step, the IGF-I step, or both steps, the zinc effect occurred at the IGF-I step. Zinc addition 4 h before the measurement of thymidine incorporation had no ameliorative effect, but the presence of zinc during the prior 12 h increased incorporation. Thus zinc exerts its major effect on DNA synthesis during the IGF-I stimulatory phase of the cell cycle. The total zinc concentration of 3T3 cells treated with DTPA for 16 h was not different from that of untreated cells; hence only a small compartment of the cell is affected by DTPA.


Assuntos
Células 3T3/efeitos dos fármacos , Células 3T3/metabolismo , Quelantes/toxicidade , DNA/biossíntese , Fator de Crescimento Insulin-Like I/farmacologia , Ácido Pentético/toxicidade , Timidina/metabolismo , Zinco/deficiência , Animais , Células Cultivadas , Fator de Crescimento Epidérmico/farmacologia , Camundongos , Fator de Crescimento Derivado de Plaquetas/farmacologia , Zinco/farmacologia , Zinco/fisiologia
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