Histamine content and mast cells distribution in mouse uterus: the effect of sexual hormones, gestation and labor.

The histamine content of uteri from mice was analyzed in terms of both concentration and total amount per uterine horn a) at two stages of the estrous cycle (estrous and diestrous), b) under sex hormone treatment, c) during pregnancy and after delivery. Histamine concentration and mast cell density were greater during diestrous and in mice treated with progesterone (p less than 0.001). This effect was attributed to a reduction in uterine mass weight, since the amount of histamine per uterine horn remained constant throughout the estrous cycle. During pregnancy, both concentration and amount of histamine per uterine horn were increased, values were significantly higher than in estrous (p less than 0.001) from day 14-17 until day 21 when labor occurred. After six to eight hours post-partum an abrupt reduction on histamine content was observed. Mast cells were more abundant in myometrium than in endometrium, their density followed the same pattern as histamine concentration throughout the estrous cycle.

Synergistic activity for high-proliferative-potential colony-forming cell (HPP-CFC) development, stable to trypsin digestion, present in pregnant mouse uterus and placenta extract.

In this paper we describe a crude pregnant mouse uterus and embryo extract (PMUE) prepared from CFW/ep mice which was able to stimulate the proliferation of high-proliferative-potential colony-forming cells (HPP-CFC) of bone marrow of normal mice, in vitro, in semisolid agar culture system. The development of that primitive murine progenitor cells requires the presence of a macrophage-stimulating factor (CSF-1) plus a synergistic factor (SF). The biological activity of both factors was present in our extracts. The higher SF activity was found in uterine plus placental tissues extracts. The SF was precipitated over 45 per cent ammonium sulfate saturation, and behaved as a nondialyzable substance, remained unaffected by trypsin digestion, and was heat-stable (70 degrees C for 15 min).

Synergistic activity for high proliferative potential colony forming cell (HPP CFC) development, stable to trypsin digestion, present in pregnant mouse uterus and placenta extract

Se estudió la ación "in vitro" del extracto de embriones y úteros de ratón preñado (PMUE), que promueve el desarrollo de grandes colonias de células progenitoras comprometidas de alto potencial prolifarativo (HPP-CFC) y su diferenciación a macrófagos. El extracto se obtuvo de la cepa CFW/ep y tanto las células de la médula ósea de ésta como las de ratones suizos normales se cultivaron en medio semisólido con agar para demostrar la acción biológica del PMUE y medio condicionante de placenta humana (HPCM). La proliferación de esas células progenitoreas muy primitivas requiere la presencia concurrente de un factor estimulante de macrófagos - CSF - y de un factor sinergístico (SF). La actividad biológica de ambos está presente en el extracto, encontrándose la mayor actividad en útero y placenta. El SF precipita con sulfato de amonio con más de 45% de saturación, no es dializable y es termiestable (a 70-C durante 15 min). Se destaca su resistencia a la acción enzimática de la tripsina

Synergistic activity for high proliferative potential colony forming cell (HPP CFC) development, stable to trypsin digestion, present in pregnant mouse uterus and placenta extract

Se estudió la ación "in vitro" del extracto de embriones y úteros de ratón preñado (PMUE), que promueve el desarrollo de grandes colonias de células progenitoras comprometidas de alto potencial prolifarativo (HPP-CFC) y su diferenciación a macrófagos. El extracto se obtuvo de la cepa CFW/ep y tanto las células de la médula ósea de ésta como las de ratones suizos normales se cultivaron en medio semisólido con agar para demostrar la acción biológica del PMUE y medio condicionante de placenta humana (HPCM). La proliferación de esas células progenitoreas muy primitivas requiere la presencia concurrente de un factor estimulante de macrófagos - CSF - y de un factor sinergístico (SF). La actividad biológica de ambos está presente en el extracto, encontrándose la mayor actividad en útero y placenta. El SF precipita con sulfato de amonio con más de 45% de saturación, no es dializable y es termiestable (a 70-C durante 15 min). Se destaca su resistencia a la acción enzimática de la tripsina (AU)

Evoluçäo da altura uterina e circunferência abdominal em gestantes normais / Evolution of uterine height and abdominal circunference in normal pregnant women

Foram analisadas retrospectivamente 180 gestantes consideradas normais clínico-laboratorialmente, atendidas no Serviço de Pré-natal do Hospital das Clínicas da Faculdade de Medicina de Ribeiräo Preto, que tiveram pelo menos 3 visitas pré-natais e deram à luz neste hospital. Observou-se que a altura uterina cresce linearmente durante a gravidez e que sua medida para uma gestaçäo a termo (padräo OMS) variou de 34 a 35cm. Na 36§ semana de gravidez, época em que normalmente se interrompem as gestaçöes de alto risco, para termos comparativos, foi de 33cm. A correlaçäo da altura uterina com idade gestacional foi quase perfeita (r = 0,93). Peso e altura fetais, assim como a circunferência abdominal, näo apresentam correlaçöes com a idade gestacional

Altura uterina como método para predecir el crecimiento fetal / Uterine height as a method of predicting fetal growth

El diagnóstico prenatal del retardo en el crecimiento intrauterino entraña dificultades que pueden llevar a la adopción de conductas inadecuadas. De ahí la búsqueda constante de métodos destinados a lograr un diagnóstico precoz y confiable. Los recientes adelantos de la medicina perinatal se apoyan en tecnologías complejas que permiten una mejor atención a los embarazos de riesgo. No obstante, el empleo de dichos métodos en casos que no lo justifican conduce a un aumento de costos, y lo que es peor al desuso de prácticas sencillas y fáciles de aplicar como es la altura uterina.Para hacer una evaluación correcta y para normalizar de forma racional la asistencia es imprescindible conocer la eficacia diagnostica de estas prácticas

[Identification of substances with biological activity similar to prostaglandins in Cestoda of Moniezia genus]. / Identificación de sustancias con actividad biológica similar a prostaglandinas en cestodos del género Moniezia.

Prostaglandins are biologically active substances which are found in large quantities in the semen of man and sheep, and in lower quantities in the amniotic and menstrual fluids, uterus, lungs, thymus, pancreas, and renal tissue. It is determined that such substances derive from unsaturated fatty acids, and that their main function is the regulation of the cell metabolism. Through the use of thin-plate chromatography as well as through the correspondent pharmacological action of the eluates upon prostaglandin-sensitive isolated organs, the presence of lipidic substances with biological activity similar to that of prostaglandins was determined for the first time ever in extracts of Moniezia cestodes. Such finding is assessed, and the possibility that the diarrhea frequently found in animals infested by such parasites may be caused by the action of their own prostaglandins on the intestinal smooth musculature of the animals, as well as the passibility that the agents inhibitory of the prostaglandin synthesis may be used in the treatment of such parasitosis are expressed.