Evaluation of thermal energy consumption in broiler farms and saving strategies / [Avaliação do consumo de energia térmica em granjas de frango e estratégias de economia]

The aim of this study was to evaluate thermal energy consumption in broiler farms and provide solutions to reduce it. This study was performed with a completely randomized design under 4 climatic conditions, including Ardabil (cold climate representative), Khuzestan (warm climate representative), Isfahan (dry climate representative) and Guilan (temperate climate representative) in 4 replicates (4 broiler farms in each climate) and with 5 repetitions (5 periods of breeding per unit) and a capacity of 492,700, Ross 308 broiler in each breeding period. According to the results, in all climates, the proposed solutions to save thermal energy were able to create a significant difference (P<0.05). The experimental results also showed that the difference in thermal energy consumption in cold and dry climates wasmuch higher than in temperate and warm climates (P<0.05). Overall, the results of the present study show that, by optimizing andmodernizing construction equipment in broiler farms, thermal energy losses can be reduced in all climatic conditions.(AU)

O objetivo do presente estudo foi avaliar o consumo de energia térmica em granjas de frangos de corte e fornecer soluções para reduzi-lo. Este estudo foi realizado com um desenho inteiramente casualizado em 4 condições climáticas, incluindo Ardabil (representante do clima frio), Khuzestan (representante do clima quente), Isfahan (representante do clima seco) e Guilan (representante do clima temperado) em 4 réplicas (4 granjas de frangos em cada clima), com 5 repetições (5 períodos de criação por unidade) e capacidade de 492.700 frangos Ross 308 em cada período de criação. De acordo com os resultados, em todos os climas, as soluções propostas para economizar energia térmica criaram diferença significativa (P <0,05). Os resultados experimentais também mostraram que a diferença no consumo de energia térmica em climas frios e secos foi muito maior do que em climas temperados e quentes (P <0,05). De forma geral, os resultados do presente estudo mostram que, com a otimização e modernização dos equipamentos de construção em granjas de frangos, as perdas de energia térmica podem ser reduzidas em todas as condições climáticas.(AU)

The epidemiology of fighting in group-housed laboratory mice.

Injurious home-cage aggression (fighting) in mice affects both animal welfare and scientific validity. It is arguably the most common potentially preventable morbidity in mouse facilities. Existing literature on mouse aggression almost exclusively examines territorial aggression induced by introducing a stimulus mouse into the home-cage of a singly housed mouse (i.e. the resident/intruder test). However, fighting occurring in mice living together in long-term groups under standard laboratory housing conditions has barely been studied. We performed a point-prevalence epidemiological survey of fighting at a research institution with an approximate 60,000 cage census. A subset of cages was sampled over the course of a year and factors potentially influencing home-cage fighting were recorded. Fighting was almost exclusively seen in group-housed male mice. Approximately 14% of group-housed male cages were observed with fighting animals in brief behavioral observations, but only 14% of those cages with fighting had skin injuries observable from cage-side. Thus simple cage-side checks may be missing the majority of fighting mice. Housing system (the combination of cage ventilation and bedding type), genetic background, time of year, cage location on the rack, and rack orientation in the room were significant risk factors predicting fighting. Of these predictors, only bedding type is easily manipulated to mitigate fighting. Cage ventilation and rack orientation often cannot be changed in modern vivaria, as they are baked in by cookie-cutter architectural approaches to facility design. This study emphasizes the need to invest in assessing the welfare costs of new housing and husbandry systems before implementing them.

Construction of an Affordable Open-Design Recirculating Zebrafish Housing System.

Recently, the zebrafish has gained in popularity as a vertebrate animal model for biomedical research. Commercial zebrafish housing systems are available and are designed to maximize stocking density of fish for a given space, but these systems are expensive and purchasing them may not be feasible for emerging laboratories with limited funding. In this article, we describe the construction of a simple and affordable recirculating zebrafish housing system. This system can be constructed in 3 working days, with materials readily available in hardware stores. The cost for construction of the system was only 3,000 MYR (750 USD). The system consists of a water reservoir, a supply line that delivers water to the shelves holding the zebrafish tanks, and a drainage line that receives water from both the supply line and the shelves containing the fish tanks and returns this water to the reservoir. This system also has a 3-stage filtration process to ensure that clean water is delivered to the zebrafish tank. The system can house up to 360 zebrafish. This low-cost housing system may make research using zebrafish feasible some laboratories.

Cost Comparison of Rodent Soiled Bedding Sentinel and Exhaust Air Dust Health-Monitoring Programs.

Rodent vivaria have traditionally used soiled bedding sentinel (SBS) health-monitoring programs to detect and exclude adventitious pathogens that could affect research results. Given the limitations of SBS, a likely reduction in animal usage, and a decrease in animal care staff labor, exhaust air dust (EAD) health monitoring has been evaluated by several groups for its efficacy in detecting pathogens when used as a complete replacement for traditional SBS health-monitoring programs. Compared with SBS, EAD has also been shown to provide increased sensitivity for the detection of multiple pathogens. After implementing EAD at our institution, we conducted an analysis to compare the annual costs of the 2 health-monitoring programs. The EAD program was found to be 26% less expensive than SBS. In addition to these cost savings, EAD decreased the amount of time spent by the staff on heath-monitoring activities. For veterinary technicians, this decrease in time was calculated as a savings of 150 h annually, almost 3 h each week. Finally, the EAD program replaced the use of live sentinel animals, decreasing the associated yearly usage from 1,676 animals to zero.

Pragmatic selection of larval mosquito diets for insectary rearing of Anopheles gambiae and Aedes aegypti.

Larval mosquitoes are aquatic omnivorous scavengers which scrape food from submerged surfaces and collect suspended food particles with their mouth brushes. The composition of diets that have been used in insectaries varies widely though necessarily provides sufficient nutrition to allow colonies to be maintained. Issues such as cost, availability and experience influence which diet is selected. One component of larval diets, essential fatty acids, appears to be necessary for normal flight though deficiencies may not be evident in laboratory cages and are likely more important when mosquitoes are reared for release into the field in e.g. mark-release-recapture and genetic control activities. In this study, four diets were compared for rearing Anopheles gambiae and Aedes aegypti, all of which provide these essential fatty acids. Two diets were custom formulations specifically designed for mosquitoes (Damiens) and two were commercially available fish foods: Doctors Foster and Smith Koi Staple Diet and TetraMin Plus Flakes. Development rate, survival, dry weight and adult longevity of mosquitoes reared with these four diets were measured. The method of presentation of one diet, Koi pellets, was additionally fed in two forms, pellets or a slurry, to determine any effect of food presentation on survival and development rate. While various criteria might be selected to choose 'the best' food, the readily-available Koi pellets resulted in development rates and adult longevity equal to the other diets, high survival to the adult stage and, additionally, this is available at low cost.

Artificial microbiome heterogeneity spurs six practical action themes and examples to increase study power-driven reproducibility.

With >70,000 yearly publications using mouse data, mouse models represent the best engrained research system to address numerous biological questions across all fields of science. Concerns of poor study and microbiome reproducibility also abound in the literature. Despite the well-known, negative-effects of data clustering on interpretation and study power, it is unclear why scientists often house >4 mice/cage during experiments, instead of ≤2. We hypothesized that this high animal-cage-density  practice abounds in published literature because more mice/cage could be perceived as a strategy to reduce housing costs. Among other sources of 'artificial' confounding, including cyclical oscillations of the 'dirty-cage/excrement microbiome', we ranked by priority the heterogeneity of modern husbandry practices/perceptions across three professional organizations that we surveyed in the USA. Data integration (scoping-reviews, professional-surveys, expert-opinion, and 'implementability-score-statistics') identified Six-Actionable Recommendation Themes (SART) as a framework to re-launch emerging protocols and intuitive statistical strategies to use/increase study power. 'Cost-vs-science' discordance was a major aspect explaining heterogeneity, and scientists' reluctance to change. With a 'housing-density cost-calculator-simulator' and fully-annotated statistical examples/code, this themed-framework streamlines the rapid analysis of cage-clustered-data and promotes the use of 'study-power-statistics' to self-monitor the success/reproducibility of basic and translational research. Examples are provided to help scientists document analysis for study power-based sample size estimations using preclinical mouse data to support translational clinical trials, as requested in NIH/similar grants or publications.

Reducing the cost and assessing the performance of a novel adult mass-rearing cage for the dengue, chikungunya, yellow fever and Zika vector, Aedes aegypti (Linnaeus).

INTRODUCTION: The widespread emergence of resistance to insecticides used to control adult Aedes mosquitoes has made traditional control strategies inadequate for the reduction of various vector populations. Therefore, complementary vector control methods, such as the Sterile Insect Technique, are needed to enhance existing efforts. The technique relies on the rearing and release of large numbers of sterile males, and the development of efficient and standardized mass-rearing procedures and tools is essential for its application against medically important mosquitoes. METHODS: In the effort to reduce the cost of the rearing process, a prototype low-cost plexiglass mass-rearing cage has been developed and tested for egg production and egg hatch rate in comparison to the current Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA) stainless-steel cage. Additionally, an adult-index was validated and used as a proxy to estimate the mosquito survival rates by counting the number of male and female mosquitoes that were resting within each of the 6 squares at a given point of time each day in the cage. RESULTS: The study has shown that the prototype mass-rearing cage is cheap and is as efficient as the FAO/IAEA stainless-steel cage in terms of egg production, with even better overall egg hatch rate. The mean numbers of eggs per cage, after seven cycles of blood feeding and egg collection, were 969,789 ± 138,101 and 779,970 ± 123,042, corresponding to 81 ± 11 and 65 ± 10 eggs per female over her lifespan, in the prototype and the stainless-steel-mass-rearing cages, respectively. The longevity of adult male and female mosquitoes was not affected by cage type and, the adult-index could be considered as an appropriate proxy for survival. Moreover, the mass-rearing cage prototype is easy to handle and transport and improves economic and logistic efficiency. CONCLUSION: The low-cost mass-rearing prototype cage can be recommended to produce Ae. aegypti in the context of rear and release techniques. The proposed adult-index can be used as a quick proxy of mosquito survival rates in mass-rearing settings.

Modeled construction and operating costs of different ventilation systems for lactating dairy cows.

The objectives were to compare capital costs of building and installation of 7 ventilation systems for adult lactating dairy cow housing and evaluate the energy use and operating cost between systems. A cost model comprising stochastic and parametric modules was created to estimate the number of fans operating each day based on temperature set points; annual profiles of daily maximum, minimum, and average temperatures; ramping functions to transition between seasons; and weather data from 7 locations in the United States. Costs were described as US$ per stall per year and operating costs as US$ (kW·h) per stall per year. Building costs amoritized over 10 yr ranged from $246 to $318, where a 16-row cross-ventilated design had the minimum cost and a hybrid design incorporating elements of tunnel and natural ventilation had the maximum cost. Lowering the summer temperature set point from 22.2 to 18.0°C to potentially improve heat abatement for high-producing cows increased cost by $10.10 (101.0 kW·h). On average, an exponential ramping function for transitioning between seasons cost $55.40 (554 kW·h) compared with $61.40 (614 kW·h) for a linear function. A tunnel barn ranged from $79.40 (794 kW·h) to $212.30 (2123 kW·h), and a natural design ranged from $32.60 (326 kW·h) to $81.80 (818 kW·h) in operating costs due to fan selection alone. Cross-ventilated barns benefitted from economies of scale and had similar operating costs as naturally ventilated barns in larger facilities. On average, mechanical systems cost twice as much to operate as natural systems, and operating costs in hotter US climates were approximately double those in milder climates. Selecting a fan with low energy efficiency can increase the operating cost of any ventilation system approximately 2-fold, making fan choice a critical design element.

"Cracking" into the debate about laying hen housing.

Livestock production systems have faced social pressures related to animal welfare; the laying hen industry also has faced changes in governing regulations/policies. This study employs experimental data to evaluate 3 confinement-housing systems: standard cages with no perches, cages with standard perches, and cages with cooled perches. Based on the cost-benefit analysis conducted of revenue less feed costs, operators are expected to have revenue, after covering feed costs, which could contribute to installation and maintenance of standard and/or cooled perches. Thus, egg producers may be able to use caged housing that incorporates perches profitably, depending on the final costs of such a system.

The allocation of resources for animal health.

Economics is too important to be left to the experts. This paper is therefore mainly for animal health policy-makers who are not economists but want a better appreciation of how economics can contribute to resource allocation decisions. First, the methodology of economic analysis is outlined with the objective of dispelling criticisms of its simplifying assumption of rationality. Then, unusual in economics but more familiar to biological and veterinary scientists, the technical aspects of transforming resources into products are discussed. Economics' unique contribution is to establish criteria enabling society to obtain maximum value from the production and distribution of goods and services (products) from scarce resources. Animal disease reduces the efficiency of this process. Value is intangible, but people reveal how much they value (i.e. feel a want or need for) products by what they actually consume, in quality and quantity. Animal products, and so implicitly animals themselves, are an example. The strength of people's preferences is reflected both in the prices they pay for market goods and services, and by their political votes where markets do not exist. Importantly, there is a difference between financial value (what the consumer pays for a good or service) and economic value (the maximum amount of money they would be prepared to pay for it). Allocating resources for animal health creates both costs and benefits, financial and economic. Moreover, costs and benefits are both private and social because of externalities, a major consideration in infectious diseases. Where production decisions with animal health implications are made exclusively for private benefit, government has a role in providing incentives for animal sectors to act in ways that result in socially efficient outcomes.

L'économie est trop importante pour être laissée entre les seules mains des experts. C'est pourquoi cet article s'adresse principalement aux responsables des politiques de santé animale qui ne sont pas économistes mais qui souhaitent néanmoins évaluer l'apport de l'économie aux décisions relatives à l'affectation des ressources. L'auteur commence par rappeler la méthodologie de l'analyse économique afin de réfuter les critiques sur le caractère supposément simplificateur du postulat de rationalité. Il examine ensuite les aspects techniques liés à la transformation de ressources en produits, concept familier pour les biologistes et les chercheurs en médecine vétérinaire mais moins courant chez les économistes. La véritable contribution de l'économie consiste à déterminer les critères qui permettent à une société de valoriser le plus possible la production et la distribution de biens et de services (produits) à partir de ressources limitées. Les maladies animales compromettent l'efficacité de ce processus. La valeur est intangible par nature mais les individus expriment la valeur qu'ils attachent à un produit (c'est-à-dire le désir ou le besoin qu'ils ont de ce produit) à travers leur consommation, au plan qualitatif et quantitatif. Les produits d'origine animale et partant, implicitement, les animaux eux-mêmes illustrent parfaitement ce phénomène. L'influence des préférences des individus se manifeste par le prix qu'ils sont disposés à payer pour les biens et les services pour lesquels il existe un marché, et par le vote politique pour tout ce qui est extérieur au marché. La distinction entre la valeur financière (prix payé par le consommateur pour un bien ou un service) et la valeur économique (le montant le plus élevé qu'il serait disposé à payer pour ce même bien ou service) est un aspect important. Les ressources allouées à la santé animale génèrent à la fois des coûts et des bénéfices, financiers et économiques. De plus, du fait des externalités, ces coûts et bénéfices sont de nature tant privée que sociale, facteur essentiel à prendre en compte pour les maladies infectieuses. Dans les situations où les décisions en matière de production animale obéissent aux seuls impératifs du profit privé, sans tenir compte des répercussions sur la santé animale, les gouvernements ont un rôle incitatif à jouer pour que le secteur de l'élevage infléchisse son action en vue de résultats efficients pour la société.

La economía es demasiado importante para abandonarla a los expertos. Por ello este artículo va dirigido sobre todo a los planificadores de políticas zoosanitarias que no son economistas pero desean tener una idea más clara de cómo puede ayudar la economía a tomar decisiones sobre la asignación de los recursos. Ante todo el autor presenta sucintamente la metodología del análisis económico, a fin de refutar las críticas que achacan una excesiva simplificación al postulado de la racionalidad. Después aborda algo inusual en economía, pero más familiar para biólogos y veterinarios: los aspectos técnicos de la transformación de los recursos en productos. La singular aportación de la economía estriba en definir criterios que permiten a la sociedad extraer el máximo «valor¼ de la producción y distribución de bienes y servicios (productos) a partir de recursos escasos. Las enfermedades animales restan eficiencia a este proceso. El «valor¼ es algo intangible, pero las personas revelan cuánto valoran un producto (es decir, hasta qué punto sienten que lo desean o lo necesitan) por lo que en la práctica consumen, tanto cualitativa como cuantitativamente. Los productos animales, y por ende, implícitamente, los propios animales, son ejemplo de ello. La fuerza de las preferencias de la gente se manifiesta en el precio que paga por bienes y servicios, cuando hay un mercado para ellos, o por su voto político, cuando no lo hay. Es importante señalar que hay una diferencia entre el valor monetario (lo que pagan los consumidores por un bien o servicio) y el valor económico (la cantidad máxima de dinero que estarían dispuestos a pagar por él). La forma en que se distribuyen los recursos en sanidad animal genera costos y beneficios, tanto monetarios como económicos. Además, esos costos y beneficios son tanto privados como sociales debido a la existencia de externalidades, factor este de gran importancia en el caso de las enfermedades infecciosas. Allí donde las decisiones de producción que tienen consecuencias zoosanitarias se rijan únicamente por el criterio del beneficio privado, las administraciones públicas deben cumplir la función de ofrecer incentivos a los sectores ligados a la producción animal para que su proceder se traduzca en resultados socialmente eficientes.

Poor construction, not time, takes its toll on subsidised fences designed to deter large carnivores.

Large carnivore conservation may be considered as successful in Sweden, as wolf (Canis lupus), lynx (Lynx lynx), brown bear (Ursus arctos), golden eagles (Aquila chrysaetos), and wolverine (Gulo gulo) populations have recovered from extinction or near extinction to viable populations during the last three decades. Particularly the wolf and lynx populations have returned at the cost of an increasing number of carnivore attacks on domestic livestock. To support coexistence between carnivores and livestock production, the Swedish authorities subsidise interventions to prevent or reduce the number of carnivore attacks. The most commonly used intervention is carnivore deterring fencing, and all livestock owners can apply for subsidies to build a fence. To receive reimbursement the fence must be approved by the authorities according to predefined criteria. An important part of any management aiming to be adaptive is evaluating interventions. In this paper we evaluate to what extent previously subsidised fences still meet the criteria 1-15 years after their approval. Of 296 fences that had received subsidies in the county of Värmland, 100 randomly selected fences were revisited in 2016. From this subsample 14% of the fences still met the initial criteria for subsidies. None of the fences that still fulfilled the criteria were more than 8 years old, whereas fences with identified failures occurred in all age groups. Of the 86 fences that failed to meet the criteria, construction failures were the most commonly occurring problem. Maintenance failures, wear and tear, only explain a minor part of the failures. To improve the quality of fencing, as well as the quality and longevity of the subsidies programme, there is a need for improved communication between authorities, and improved communication and support from the authorities to livestock producers before and during construction of fences, as well as more rigorous inspection when the fences are built.

Versatile 3D-printed headstage implant for group housing of rodents.

BACKGROUND: An unfavourable yet necessary side-effect of stereotaxic surgery involves the social isolation of post-surgery rats, in order to protect their wound site or skull-mounted implant from damage. Social isolation can cause a myriad of behavioural and physiological changes that are detrimental to the well-being of rats, with potential negative implications for a range of experimental paradigms. New method. Female Sprague Dawley rats (n=40) were implanted onto the skull with a novel 3D-printed headstage socket that surrounded an electrode connector. The socket accommodated a removable stainless-steel headcap for the purposes of protecting the implant. Rats were pair-housed following surgery, and their behaviour was monitored for up to several weeks under two experimental conditions that involved EEG recording and deep-brain stimulation, as well as behavioural test sessions inside an open-field maze. Rat weights were compared between individually- and pair-housed rats at up to 3 weeks post-surgery. RESULTS: These experiments were successfully carried out using pair-housed rats, with no damage or complications observed regarding the implant and its headcap. Rats were able to carry out a range of normal behaviours including running, grooming, foraging and sleeping. Compared to individually-housed rats, pair-housed rats gained less weight over the 3 weeks post-implantation period. Comparison with existing method(s). This method offers additional protection compared to group-housed post-surgical rats that lack the protective headcap. It is also potentially more practical and versatile than a fully-implantable device for the safe post-surgery group housing of rodents. CONCLUSIONS: This implant design can reduce the cost of rodent upkeep, whilst potentially avoiding a myriad of behavioural and physiological changes that are known to result from social isolation.

Hygieia Comfort Cages--better for the research mouse, better for your research dollars.

Investigative research is the foundation of innovation that drives a first-world quality of life. Because we stand on the shoulders of scientific giants, we live longer, healthier and more productive lives than any humans in the history of civilization. The laboratory mouse is one tool that enables innovation to move forward, with 20-30 million mice used across the US each year. Many variables contribute to the environment surrounding the laboratory mouse in an individually ventilated cage (IVC), such as the type of cage used, the composition and volume of bedding used, the number of air changes per hour (ACH), the frequency of bedding changes, the frequency of cage changes and the temperature and relative humidity of air in the cage.

Sensitivity analysis to aid shelter management decisions: how does altering expenditure affect operational viability?

Streamlining purchasing in nonhuman animal shelters can provide multiple financial benefits. Streamlining shelter inputs and thus reducing shelter costs can include trading paid labor and management for fewer, more involved volunteers or purchasing large quantities of medical supplies from fewer vendors to take advantage of bulk-purchasing discounts. Beyond direct savings, time and energy spent on purchasing and inventory control can be reduced through careful management. Although cost-cutting measures may seem attractive, shelter managers are cautioned to consider the potential unintended consequences of short-term cost reduction measures that could limit revenues or increase costs in the future. This analysis illustrates an example of the impact of cost reductions in specific expense categories and the impact on shelter net revenue, as well as the share of expenses across categories. An in-depth discussion of labor and purchasing cost-reducing strategies in the real world of animal shelter management is provided.

Effects of housing system on the costs of commercial egg production.

This article reports the first publicly available egg production costs compared across 3 hen-housing systems. We collected detailed data from 2 flock cycles from a commercial egg farm operating a conventional barn, an aviary, and an enriched colony system at the same location. The farm employed the same operational and accounting procedures for each housing system. Results provide clear evidence that egg production costs are much higher for the aviary system than the other 2 housing systems. Feed costs per dozen eggs are somewhat higher for the aviary and lower for the enriched house compared with the conventional house. Labor costs are much lower for the conventional house than the other 2, and pullet costs are much higher for the aviary. Energy and miscellaneous costs are a minimal part of total operating costs and do not differ by housing system. Total capital investments per hen-capacity are much higher for the aviary and the enriched house. Capital costs per dozen eggs depend on assumptions about appropriate interest and depreciation rates. Using the same 10% rate for each housing system shows capital costs per dozen for the aviary and the enriched housing system are much higher than capital costs per dozen for the conventional house. The aviary has average operating costs (feed, labor, pullet, energy, and miscellaneous costs that recur for each flock and vary with egg production) about 23% higher and average total costs about 36% higher compared with the conventional house. The enriched housing system has average operating costs only about 4% higher compared with the conventional house, but average total costs are 13% higher than for the conventional house.

Construction of an affordable and easy-to-build zebrafish facility.

In vivo biomedical research is pivotal to translate in vitro findings into clinical advances. Small academic institutions with limited resources find it virtually impossible to build and maintain typical rodent facilities for research. Zebrafish research has been demonstrated to be a valuable alternative for in vivo research in pharmacology, physiology, development and genetic studies. This article demonstrates that a functional zebrafish facility can be built in an easy and affordable manner. We demonstrate that such a facility could be built in about one working day with minimal tools and expertise. The cost of the 27 1.8 L fish tank zebrafish facility constructed in this study was approximately $1,500. We estimate that the maintenance of an initial working 150 fish colony for 3 months is $1,000. This project involved students, who were introduced to aquaculturing of zebrafish for research proposes.

Evaluation of the sustainability of contrasted pig farming systems: the procedure, the evaluated systems and the evaluation tools.

Although a few studies consider the sustainability of animal farming systems along the three classical main pillars (economy, environment and society), most studies on pig farming systems address only one of these pillars. The present paper is the introduction to a series of companion papers presenting the results of a study undertaken within the EU-supported project Q-PorkChains, aiming at building a comprehensive tool for the evaluation of pig farming systems, which is robust to accommodate the large variability of systems existing in Europe. The tool is mostly based on questions to farmers and comprises a total of 37 dimensions distributed along eight themes: Animal Welfare, Animal Health, Breeding Programmes, Environmental Sustainability, Meat Safety, Market Conformity, Economy and Working Conditions. The paper describes the procedure that was used for building the tool, using it on 15 contrasted pig farming systems and analysing the results. The evaluated systems are briefly described and a short overview of the dimensions is provided. Detailed descriptions of the theme-wise tools and results, as well as the results of an integrated evaluation, are available in the companion papers.