Financial losses for Dutch stakeholders during the 2013 aflatoxin incident in Maize in Europe.

Early 2013, high concentrations of aflatoxin M1 were found in the bulk milk of a few dairy farms in the Netherlands. These high concentrations were caused by aflatoxin B1 contaminated maize from Eastern Europe that was processed into compound feed, which was fed to dairy cows. Since the contamination was discovered in the downstream stages of the supply chain, multiple countries and parties were involved and recalls of the feed were necessary, resulting into financial losses. The aim of this study was to estimate the direct short-term financial losses related to the 2013 aflatoxin incident for the maize traders, the feed industry, and the dairy sector in the Netherlands. First, the sequence of events of the incident was retrieved. Then, a Monte Carlo simulation model was built to combine the scarce and uncertain data to estimate the direct financial losses for each stakeholder. The estimated total direct financial losses of this incident were estimated to be between 12 and 25 million euros. The largest share, about 60%, of the total losses was endured by the maize traders. About 39% of the total losses were for the feed industry, and less than 1% of the total losses were for the dairy sector. The financial losses estimated in this study should be interpreted cautiously due to limitations associated with the quality of the data used. Furthermore, this incident led to indirect long-term financial effects, identified but not estimated in this study.

Costs and efficacy of public health interventions to reduce aflatoxin-induced human disease.

This study reviews available information on the economics and efficacy of aflatoxin risk-reduction interventions, and it provides an approach for analysis of the cost-effectiveness of public health interventions to reduce aflatoxin-induced human disease. Many strategies have been developed to reduce aflatoxin or its adverse effects in the body. However, a question that has been under-addressed is how likely these strategies will be adopted in the countries that need them most to improve public health. This study evaluates two aspects crucial to the adoption of new technologies and methods: the costs and the efficacy of different strategies. First, different aflatoxin risk-reduction strategies are described and categorized into pre-harvest, post-harvest, dietary, and clinical settings. Relevant data on the costs and efficacy of each strategy, in reducing either aflatoxin in food or its metabolites in the body are then compiled and discussed. In addition, we describe which crops are affected by each intervention, who is likely to pay for the control strategy, and who is likely to benefit. A framework is described for how to evaluate cost-effectiveness of strategies according to World Health Organization (WHO) standards. Finally, it is discussed which strategies are likely to be cost-effective and helpful under different conditions worldwide of regulations, local produce and soil ecology, and potential health emergencies.

Mycotoxin reduction in Bt corn: potential economic, health, and regulatory impacts.

Genetically modified (GM) Bt corn, through the pest protection that it confers, has lower levels of mycotoxins: toxic and carcinogenic chemicals produced as secondary metabolites of fungi that colonize crops. In some cases, the reduction of mycotoxins afforded by Bt corn is significant enough to have an economic impact, both in terms of domestic markets and international trade. In less developed countries where certain mycotoxins are significant contaminants of food, Bt corn adoption, by virtue of its mycotoxin reduction, may even improve human and animal health. This paper describes an integrated assessment model that analyzes the economic and health impacts of two mycotoxins in corn: fumonisin and aflatoxin. It was found that excessively strict standards of these two mycotoxins could result in global trade losses in the hundreds of millions US dollars annually, with the US, China, and Argentina suffering the greatest losses. The paper then discusses the evidence for Bt corn's lower levels of contamination of fumonisin and aflatoxin, and estimates economic impacts in the United States. A total benefit of Bt corn's reduction of fumonisin and aflatoxin in the US was estimated at 23 million dollars annually. Finally, the paper examines the potential policy impacts of Bt corn's mycotoxin reduction, on nations that are making a decision on whether to allow commercialization of this genetically modified crop.

Mycotoxin risk assessment for the purpose of setting international regulatory standards.

The 2003 Council for Agricultural Science and Technology Mycotoxin report states that one 21st century goal is the development of uniform regulations worldwide for foodborne mycotoxin contamination. This study informs that endeavor by a risk assessment and economic analysis of two important mycotoxins: fumonisins and aflatoxins. The goals are to identify the nations that would be most heavily impacted by tighter mycotoxin regulations, examine costs and benefits as a function of regulatory stringency, and address risk-risk tradeoffs between health benefits and economic losses from compliance with those regulations. Among industrial nations, the United States would experience the heaviest economic losses from more precautionary mycotoxin standards. Environmental conditions in the developing world, however, are more conducive to mycotoxin accumulation in crops. Contrary to concerns expressed among policymakers, the less developed countries that would likely experience the greatest loss from tighter mycotoxin standards are not sub-Saharan African nations, but China and Argentina. If a fumonisin standard of 0.5 mg/kg were adopted worldwide, total export losses from fumonisins in corn may exceed 300 million dollars annually: 3-fold higher than if the less stringent U.S. standard of 2 mg/kg were adopted. Likewise, export losses from aflatoxins in peanuts may exceed 450 million dollars under the current EU regulatory standard of 4 microg/kg: almost 5-fold higher than if the U.S. standard of 20 microg/kg were adopted. Stricter standards are unlikely to improve health significantly. In developing nations such as China where hepatitis B and C are prevalent, tighter aflatoxin standards may increase health risks until improved control methods for aflatoxins are found, as high-quality crops may be exported instead of being consumed domestically.

Production of ultrasensitive antibodies against aflatoxin B1.

AIMS: To produce specific antibodies against the haptenic fungal toxin aflatoxin B1 (AFB1) and apply these antibodies in immunochemical assays for aflatoxins. METHODS AND RESULTS: Rabbits were immunized using an AFB1-bovine serum albumin conjugate and serum titres determined by double-antibody enzyme immunoassay. High titres of antibodies with very high affinity for AFB1 were obtained 15 and 4 weeks after the initial immunization and the first booster immunization respectively. The antibodies were employed in enzyme immunoassay (EIA) and immunoaffinity chromatography (IAC) methods for aflatoxins. With a detection limit of 15.8 pg ml(-1) for AFB1, the EIA employing these antibodies is the most sensitive test for AFB1 described so far. In IAC columns, these antibodies provided high binding capacity for all major aflatoxins, including AFB1, AFB2, AFG1 and AFG2. CONCLUSION: The antibodies described here are useful for the analysis of trace levels of aflatoxins. SIGNIFICANCE AND IMPACT OF THE STUDY: Polyclonal antibody-based EIA and IAC methods for aflatoxin analysis offer a suitable alternative to the more expensive monoclonal antibody-based methods.