Detección de los cambios fenotípicos en productos de ratas long evans infectadas con trichinella spiralis y tratadas con albendazol / Phenotypic changes detection in new born Long Evans Rat infected with T. spiralis and Albendazol treated

Trichinella spiralis es uno de los agentes causantes de la Trichinellosis, es una zoonosis de distribución mundial, afecta a gran número de mamíferos así como al humano. En México la causa principal de infección se asocia con el consumo de carne de cerdo infectada con T. spiralis. En este trabajo se detectaron los cambios fenotípicos en productos de ratas Long Evans infectas con T. spiralis y tratadas con Albendazol. Utilizamos ratas cepa Long Evans en edad reproductiva. Divididas en grupos, incluidos controles y con tratamiento de uno a catorce días de tratamiento, para sincronizar la gestación en las ratas se realizaron frotis vaginales. Para la detección del parásito se utilizaron técnicas directas de compresión en placa y digestión artificial y la técnica indirecta de MIDD, para los cambios fenotípicos de crías obtenidas se realizo análisis detallado con microscopio estereoscópico

Trichinella spiralis is one of the causative agents of the Trichinellosis, is a zoonosis of world-wide distribution, affects to big number of mammals like this as to the human. In Mexico the main cause of infection associate with the consumption of pig infected meat with T. spiralis. In this work detected the phenotypical changes in long Evans rats products infected with Trichinells spiralis and threated with albendazol medication. Use rats cepa long evans ina reproductive age. Divided in groups, included controls and with treatment varying of one to fourteen days of treatment, to synchronize the management in the rats realized vaginal.smears For the detection of the parasites used direct techniques of understanding in plate and artificial digestion and the indirect technician of MIID, for the phenotypical changes of baby rats obtained realized a detailed analysis with stereoscopic microscope

Intestinal elimination of albendazole sulfoxide: pharmacokinetic effects of inhibitors.

Albendazole (ABZ) is an anthelmintic drug widely used in human and veterinary medicine. Intestinal and hepatic ABZ metabolism leads to albendazole sulfoxide (ABZSO), the active metabolite. This work examines the mechanism involved in intestinal elimination of ABZSO and their pharmacokinetic consequences in rat and sheep. To assess the drug intestinal elimination, an upper small intestine segment was isolated and perfused in situ with saline, after ABZSO administration (10 mg/kg i.v.). The intestinal clearance of ABZSO was 0.106+/-0.010 ml/min, exhibiting a stereoselective intestinal elimination to (-)ABZSO form. Oxfendazole, ampicillin and cyclosporine significantly reduced the intestinal elimination of ABZSO to 0.079+/-0.008, 0.069+/-0.009 and 0.065+/-0.012 ml/min, respectively. Glucose significantly induced ABZSO intestinal elimination. Pharmacokinetic results showed a clear and statistically significant interaction between ABZ metabolites and drug efflux inhibitors. In rat, an increased area under the curve (AUC) for ABZSO in the groups co-administered with ABZ plus verapamil (43%) and plus ketoconazole (29%) was obtained. In sheep, the AUC for ABZSO in the groups co-administered with the inhibitors were significantly higher 53.68% with verapamil, 78.62% with quinidine, and 50.55% with ivermectin.