1.
Clin Transl Med
; 11(11): e572, 2021 11.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34841699
Assuntos
Aminoácido Oxirredutases/farmacologia , Antifibróticos/farmacologia , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/normas , Aminoácido Oxirredutases/análise , Aminoácido Oxirredutases/uso terapêutico , Antifibróticos/análise , Antifibróticos/uso terapêutico , Técnicas Biossensoriais/métodos , Fibrose/tratamento farmacológico , Fibrose/prevenção & controle , Humanos
2.
STAR Protoc
; 2(1): 100387, 2021 03 19.
Artigo
em Inglês
| MEDLINE
| ID: mdl-33778781
RESUMO
Controversies remain over the standard procedures for the modeling of skin fibrosis and its use in in vitro testing of different drugs. Here, we report a reproducible protocol for producing a skin fibrosis model using human dermal fibroblasts seeded in collagen hydrogel. Detailed procedures for the fabrication of cell/hydrogel constructs, fibrosis induction, protein extraction for western blotting analysis are presented along with how this model can be employed for investigating the possible anti-fibrotic functions of certain chemical compounds.