RESUMO
The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 µg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 µg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 µg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E(2 )(PGE(2)) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 µg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research.
Assuntos
Arisaema/química , Lectinas de Plantas/imunologia , Lectinas de Plantas/isolamento & purificação , Lectinas de Plantas/farmacologia , Animais , Arisaema/anatomia & histologia , Assialoglicoproteínas/farmacologia , Movimento Celular/efeitos dos fármacos , Dinoprostona/imunologia , Edema/induzido quimicamente , Edema/patologia , Eritrócitos/efeitos dos fármacos , Fetuínas/farmacologia , Hemaglutinação/efeitos dos fármacos , Macrófagos Peritoneais/citologia , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/metabolismo , Masculino , Mastócitos/citologia , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Metacarpo/patologia , Neutrófilos/citologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/fisiologia , Óxido Nítrico/imunologia , Raízes de Plantas/química , Coelhos , Ratos , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa/imunologia , p-Metoxi-N-metilfenetilamina/farmacologiaRESUMO
Through textual research and surveying on officinal varieties of Rhizoma Arisaematis, we considered the following results. The name of Rhizoma Arisaematis is in ceaseless change and its original is in confusion in the development of bencaology history. The original plants as Rhizoma Arisaematis are from many species and have wide distribution. This review can provide important reference for exploitation and utilization of resources and further development of Rhizoma Arisaematis through the discussion of the state of its original plant, distribution and mainstream varieties in the current market.
