RESUMO
OBJECTIVE: Quantitative relationships between the extent of injury and thrombus formation in vivo are not well understood. Moreover, it has not been investigated how increased injury severity translates to blood-flow modulation. Here, we investigated interconnections between injury length, clot growth, and blood flow in a mouse model of laser-induced thrombosis. Approach and Results: Using intravital microscopy, we analyzed 59 clotting events collected from the cremaster arteriole of 14 adult mice. We regarded injury length as a measure of injury severity. The injury caused transient constriction upstream and downstream of the injury site resulting in a 50% reduction in arteriole diameter. The amount of platelet accumulation and fibrin formation did not depend on arteriole diameter or deformation but displayed an exponentially increasing dependence on injury length. The height of the platelet clot depended linearly on injury length and the arteriole diameter. Upstream arteriolar constriction correlated with delayed upstream velocity increase, which, in turn, determined downstream velocity. Before clot formation, flow velocity positively correlated with the arteriole diameter. After the onset of thrombus growth, flow velocity at the injury site negatively correlated with the arteriole diameter and with the size of the above-clot lumen. CONCLUSIONS: Injury severity increased platelet accumulation and fibrin formation in a persistently steep fashion and, together with arteriole diameter, defined clot height. Arterial constriction and clot formation were characterized by a dynamic change in the blood flow, associated with increased flow velocity.
Assuntos
Músculos Abdominais/irrigação sanguínea , Arteríolas/patologia , Coagulação Sanguínea , Trombose/patologia , Lesões do Sistema Vascular/patologia , Animais , Arteríolas/lesões , Arteríolas/fisiopatologia , Velocidade do Fluxo Sanguíneo , Plaquetas/metabolismo , Constrição Patológica , Modelos Animais de Doenças , Fibrina/metabolismo , Microscopia Intravital , Masculino , Camundongos , Microscopia de Fluorescência , Índice de Gravidade de Doença , Trombose/sangue , Trombose/fisiopatologia , Fatores de Tempo , Lesões do Sistema Vascular/sangue , Lesões do Sistema Vascular/fisiopatologiaRESUMO
Noninflammatory necrotizing vasculopathy, also referred to as lupus vasculopathy, is not infrequently observed in the pathology of lupus nephritis. It affects vessels causing them to become severely narrowed and occluded by a mechanism involving immune complexes. We experienced a 51-year-old woman with lupus nephritis class IV + V, which was accompanied by lupus vasculopathy. Renal biopsy and light microscopy showed eosinophilic hyaline-like material in the afferent and/or efferent arterioles, which narrowed the lumen, and which were positive for IgG by immunofluorescent analysis. Electron microscopy indicated that amorphous material and endothelial detachment occluded the arterioles. These findings were consistent with those of lupus vasculopathy. We treated the patient with steroids and cyclophosphamide. By the day of discharge, her levels of creatinine and proteinuria had undergone partial remission. Although lupus vasculopathy was implied as a lesion with unfavorable renal prognosis, some recent reports suggest its true renal prognosis is not unfavorable necessarily. Nevertheless, lupus vasculopathy is an important finding in diagnosis in contradiction to other vascular legions in systemic lupus erythematosus. In addition, a standard therapy has also not been established. Therefore, it is important to accumulate cases of lupus vasculopathy to determine its prognosis and develop standard treatments.
Assuntos
Arteríolas/lesões , Rim/irrigação sanguínea , Nefrite Lúpica/complicações , Doenças Vasculares/etiologia , Corticosteroides/administração & dosagem , Corticosteroides/uso terapêutico , Arteríolas/patologia , Biópsia , Creatinina/sangue , Ciclofosfamida/administração & dosagem , Ciclofosfamida/uso terapêutico , Quimioterapia Combinada , Imunofluorescência/métodos , Humanos , Imunossupressores/administração & dosagem , Imunossupressores/uso terapêutico , Rim/patologia , Nefrite Lúpica/diagnóstico , Nefrite Lúpica/imunologia , Nefrite Lúpica/patologia , Microscopia Eletrônica/métodos , Pessoa de Meia-Idade , Prognóstico , Proteinúria/urina , Doenças Vasculares/tratamento farmacológico , Doenças Vasculares/patologiaRESUMO
An increase in arterial pressure within the cerebral vasculature appears to coincide with ischemia and dysfunction of the neurovascular unit in some cases of traumatic brain injury. In this study, we examine a new mechanism of brain tissue damage that results from excessive cerebral arteriole pressurization. We begin by considering the morphological and material properties of normotensive and hypertensive arterioles and present a computational model that captures the interaction of neighboring pressurized arterioles and the surrounding brain tissue. Assuming an axonal strain-induced injury criterion, we find that the injury depends on vessel spacing, proximity to an unconfined free surface, and the relative difference in stiffness between the arterioles and the surrounding tissue. We find that a steeper heterogeneity (stiffer vessels surrounded by softer brain tissue) causes larger axial strains to develop at some distance from the arteriole wall, within the brain parenchyma. For a more gradual heterogeneity (softer vessels), we observe more larger strain fields close to the arteriole walls. Both deformation patterns are comparable to damage seen in previous pathology studies on postmortem TBI patients. Finally, we use an analytical model to approximate the interplay between internal pressure, arteriole thickness, and the variation in mechanical properties of arterioles.
Assuntos
Arteríolas/lesões , Encéfalo/irrigação sanguínea , Animais , Anisotropia , Pressão Arterial , Arteríolas/patologia , Arteríolas/fisiopatologia , Encéfalo/fisiopatologia , Lesões Encefálicas Traumáticas/patologia , Lesões Encefálicas Traumáticas/fisiopatologia , Córtex Cerebral/patologia , Simulação por Computador , Módulo de Elasticidade , Análise de Elementos Finitos , Humanos , Modelos Biológicos , Ratos , Estresse MecânicoRESUMO
BACKGROUND/AIMS: Cardiovascular complications are responsible for increased mortality and morbidity in chronic kidney disease (CKD) patients. Functional and structural changes of peritoneal membrane are reported in CKD patients both on conservative treatment and on renal replacement therapy (RRT). The aim of the study was to assess the structure of peritoneal membrane small arteries (precapillary arterioles) in diabetic and non-diabetic CKD stage 5 patients before initiation of peritoneal dialysis (PD) and evaluate its relationship with heart and large arteries abnormalities and with selected biochemical parameters. METHODS: Evaluation of 42 CKD stage 5 patients before starting PD. Diabetic (n=26) and non-diabetic (n=16) patients were compared. Peritoneal membrane samples were taken during Tenckhoff catheter insertion. Histopathological evaluation of peritoneal precapillary arterioles (arteriolar evaluation) with measurement of wall thickness (WT) and calculation of lumen/vessel (L/V) ratio was performed in each patients. Echocardiography, intima media thickness (IMT), pulse wave velocity (PWV), ambulatory blood pressure monitoring (ABPM) and biochemical parameters assessment: serum albumin (SA), total cholesterol (TCH), hemoglobin (Hgb), parathormone (PTH), serum calcium (Ca), serum phosphorus (P), transferrin saturation (TSAT%), C-reactive protein (CRP) were performed in each participant. RESULTS: There were no statistically significant differences in peritoneal membrane arteriolar indices - wall thickness (WT) and L/V ratio between investigated groups. There was statistically significant higher PWV value in diabetic patients. There were no statistically significant differences in echocardiographic indices, IMT, laboratory data in analyzed groups. There were some linear correlations between: PWV vs IMT (R=0,84; p=0,0006); PWV vs PP (R=0,58; p=0,03) in non-diabetic and linear correlation between: PWV vs age (R=0,75; p=0,02); WT vs DP (R=-0,93; p=0,001); WT vs DBP ( R=0,64; p=0,04) in diabetic group. CONCLUSION: Peritoneal membrane arteriolar damage seems to be an integrated part of cardiovascular system damage in CKD stage 5 patients.
Assuntos
Arteríolas/patologia , Doenças Cardiovasculares/diagnóstico , Membranas/irrigação sanguínea , Peritônio/ultraestrutura , Insuficiência Renal Crônica/complicações , Adulto , Idoso , Arteríolas/lesões , Arteríolas/ultraestrutura , Doenças Cardiovasculares/mortalidade , Espessura Intima-Media Carotídea , Diabetes Mellitus , Humanos , Pessoa de Meia-Idade , Análise de Onda de Pulso , Insuficiência Renal Crônica/mortalidadeRESUMO
Essentials Platelet packing density in a hemostatic plug limits molecular movement to diffusion. A diffusion-dependent steep thrombin gradient forms radiating outwards from the injury site. Clot retraction affects the steepness of the gradient by increasing platelet packing density. Together, these effects promote hemostatic plug core formation and inhibit unnecessary growth. SUMMARY: Background Hemostasis studies performed in vivo have shown that hemostatic plugs formed after penetrating injuries are characterized by a core of highly activated, densely packed platelets near the injury site, covered by a shell of less activated and loosely packed platelets. Thrombin production occurs near the injury site, further activating platelets and starting the process of platelet mass retraction. Tightening of interplatelet gaps may then prevent the escape and exchange of solutes. Objectives To reconstruct the hemostatic plug macro- and micro-architecture and examine how platelet mass contraction regulates solute transport and solute concentration in the gaps between platelets. Methods Our approach consisted of three parts. First, platelet aggregates formed in vitro under flow were analyzed using scanning electron microscopy to extract data on porosity and gap size distribution. Second, a three-dimensional (3-D) model was constructed with features matching the platelet aggregates formed in vitro. Finally, the 3-D model was integrated with volume and morphology measurements of hemostatic plugs formed in vivo to determine how solutes move within the platelet plug microenvironment. Results The results show that the hemostatic mass is characterized by extremely narrow gaps, porosity values even smaller than previously estimated and stagnant plasma velocity. Importantly, the concentration of a chemical species released within the platelet mass increases as the gaps between platelets shrink. Conclusions Platelet mass retraction provides a physical mechanism to establish steep chemical concentration gradients that determine the extent of platelet activation and account for the core-and-shell architecture observed in vivo.
Assuntos
Músculos Abdominais/irrigação sanguínea , Arteríolas/lesões , Plaquetas/metabolismo , Hemostasia , Agregação Plaquetária , Trombina/metabolismo , Trombose/sangue , Lesões do Sistema Vascular/sangue , Animais , Arteríolas/patologia , Arteríolas/fisiopatologia , Velocidade do Fluxo Sanguíneo , Plaquetas/patologia , Retração do Coágulo , Simulação por Computador , Difusão , Modelos Animais de Doenças , Camundongos Endogâmicos C57BL , Microcirculação , Modelos Biológicos , Porosidade , Trombose/patologia , Trombose/fisiopatologia , Fatores de Tempo , Lesões do Sistema Vascular/patologia , Lesões do Sistema Vascular/fisiopatologiaRESUMO
Stem cell-derived platelets have the potential to replace donor platelets for transfusion. Defining the platelet-producing megakaryocytes (MKs) within the heterogeneous MK culture may help to optimize the in vitro generation of platelets. Using 2 human stem cell models of megakaryopoiesis, we identified novel MK populations corresponding to distinct maturation stages. An immature, low granular (LG) MK pool (defined by side scatter on flow cytometry) gives rise to a mature high granular (HG) pool, which then becomes damaged by apoptosis and glycoprotein Ib α chain (CD42b) shedding. We define an undamaged HG/CD42b+ MK subpopulation, which endocytoses fluorescently labeled coagulation factor V (FV) from the media into α-granules and releases functional FV+CD42b+ human platelet-like particles in vitro and when infused into immunodeficient mice. Importantly, these FV+ particles have the same size distribution as infused human donor platelets and are preferentially incorporated into clots after laser injury. Using drugs to protect HG MKs from apoptosis and CD42b shedding, we also demonstrate that apoptosis precedes CD42b shedding and that apoptosis inhibition enriches the FV+ HG/CD42b+ MKs, leading to increased platelet yield in vivo, but not in vitro. These studies identify a transition between distinct MK populations in vitro, including one that is primed for platelet release. Technologies to optimize and select these platelet-ready MKs may be important to efficiently generate functional platelets from in vitro-grown MKs.
Assuntos
Plaquetas/citologia , Células da Medula Óssea/imunologia , Fator V/genética , Células Progenitoras de Megacariócitos/citologia , Megacariócitos/citologia , Animais , Apoptose/efeitos dos fármacos , Arteríolas/efeitos dos fármacos , Arteríolas/imunologia , Arteríolas/lesões , Biomarcadores/sangue , Plaquetas/imunologia , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Diferenciação Celular , Linhagem da Célula/imunologia , Endocitose , Fator V/imunologia , Fator V/farmacologia , Citometria de Fluxo , Expressão Gênica , Humanos , Imunofenotipagem , Lasers , Células Progenitoras de Megacariócitos/imunologia , Megacariócitos/imunologia , Camundongos , Camundongos SCID , Complexo Glicoproteico GPIb-IX de Plaquetas/genética , Complexo Glicoproteico GPIb-IX de Plaquetas/imunologiaRESUMO
Introducción. El reflejo venoarteriolar (RVA) lo provoca un incremento en la presión venosa transmural al colocar una parte del cuerpo en el sentido de la aceleración gravitatoria por debajo del corazón. Objetivo. Evaluar el RVA en sujetos sanos al levantar una parte del cuerpo por encima del corazón. Sujetos y métodos. En 16 sujetos sanos (20-65 años) se estudió el RVA mediante cambios en el flujo sanguíneo de la piel con un fotopletismógrafo digital infrarrojo colocado en el pulpejo en sujetos sanos durante las siguientes condiciones: brazo derecho a la altura del corazón, brazo derecho 40 cm por debajo del corazón y brazo derecho 40 cm por encima del corazón. Las variables medidas fueron: amplitud del flujo sanguíneo de la piel con el brazo a la altura del corazón (amplitud basal), porcentaje de disminución del flujo sanguíneo de la piel con el brazo por debajo del corazón y porcentaje de aumento del flujo sanguíneo de la piel con el brazo por encima del corazón. Resultados. El porcentaje de vasoconstricción con el brazo derecho por debajo del corazón fue del 35%, y el de vasodilatación, del 50%. Conclusiones. La evaluación del RVA con el brazo por debajo del corazón provoca vasoconstricción, y la elevación del brazo produce una importante vasodilatación. La vasoconstricción y la vasodilatación se mantienen mientras la extremidad se mantenga por encima o por debajo del corazón. Éste es un estudio potencialmente muy útil y económico para estudiar la inervación de la microcirculación en diversas neuropatías periféricas de fibras delgadas y mixtas (AU)
Introduction. The veno-arteriolar reflex (VAR) is triggered by an increase in the transmural venous pressure on placing a part of the body in the same direction as the gravitational acceleration below the heart. Aim. To assess the VAR in healthy subjects on raising a part of the body above the level of the heart. Subjects and methods. VAR was studied in 16 healthy subjects (20-65 years old) by means of changes in the blood flow in the skin detected using a digital infrared photoplethysmograph attached to the fingertip under the following conditions: right arm at the height of the heart, right arm below the heart and right arm below the level of the heart. The variables measured were: amplitude of the blood flow in the skin with the arm raised to the height of the heart (baseline amplitude), percentage decrease of the blood flow in the skin with the arm below the heart and percentage increase in blood flow with the arm above the heart. Results. The percentage of vasoconstriction with the right arm below the heart was 35%, and that of vasodilation, 50%. Conclusions. Evaluation of the VAR with the arm below the heart causes vasoconstriction, and elevation of the arm causes an important degree of vasodilation. Vasoconstriction and vasodilation are maintained while the limb is kept above or below the heart. This is an economical and potentially very useful way of studying the innervation of the microcirculation in a number of different peripheral neuropathies of thin and mixed fibres (AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Vasoconstrição/fisiologia , Vasodilatação/fisiologia , Microcirculação/fisiologia , Arteríolas/lesões , Arteríolas/patologia , Homeostase/fisiologia , Sistema Nervoso Autônomo/lesões , Sistema Nervoso Autônomo/patologia , Sistema Nervoso Autônomo/fisiologia , Sistema Nervoso Simpático/lesões , Sistema Nervoso Simpático/patologia , Sistema Nervoso Simpático/fisiologia , Fotopletismografia/instrumentação , Fotopletismografia/métodos , Fotopletismografia , Ultrassonografia Doppler/instrumentação , Ultrassonografia Doppler/métodos , Ultrassonografia Doppler , Termografia/instrumentação , Termografia/métodos , TermografiaAssuntos
Doenças das Cartilagens/etiologia , Telefone Celular , Dermatite/etiologia , Otopatias/etiologia , Orelha Externa/lesões , Doenças Profissionais/etiologia , Arteríolas/lesões , Doenças das Cartilagens/diagnóstico , Dermatite/diagnóstico , Cartilagem da Orelha/irrigação sanguínea , Otopatias/diagnóstico , Orelha Externa/irrigação sanguínea , Humanos , Isquemia/etiologia , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/diagnóstico , Pressão/efeitos adversos , Meios de Transporte , Vasculite/etiologiaRESUMO
An important negative regulator of factor VIIIa (FVIIIa) cofactor activity is A2 subunit dissociation. FVIII molecules with stabilized activity have been generated by elimination of charged residues at the A1-A2 and A2-A3 interfaces. These molecules exhibited reduced decay rates as part of the enzymatic factor Xa generation complex and retained their activities under thermal and chemical denaturing conditions. We describe here the potency and efficacy of 1 such stability variant, D519V/E665V, derived from B domain-deleted FVIII (BDD-FVIII). The major effect of A2 stabilization was on cofactor activity. D519V/E665V potency was increased twofold by the 2-stage chromogenic assay relative to BDD-FVIII. D519V/E665V demonstrated enhanced thrombin generation responses (fivefold by peak thrombin) relative to BDD-FVIII. In vivo consequences of enhanced cofactor activity of D519V/E665V included >fourfold increased maximal platelet-fibrin deposition after laser injury and twofold increased protection from bleeding in acute and prolonged vascular injury model in hemophilia A mice. These results demonstrate that noncovalent stabilization of the FVIII A2 subunit can prolong its cofactor activity, leading to differential enhancement in clot formation over protection from blood loss in hemophilia. The FVIII molecule described here is the first molecule with clear efficacy enhancement resulting from noncovalent stabilization of the A2 domain.
Assuntos
Fator VIII/química , Fator VIII/farmacologia , Hemofilia A/genética , Animais , Arteríolas/lesões , Modelos Animais de Doenças , Fator VIII/genética , Feminino , Camundongos , Camundongos Knockout , Estabilidade Proteica , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/farmacologiaRESUMO
The role of salt intake in the development of hypertension is prominent, but its mechanism has not been fully elucidated. Our aim was to examine the effect of transient salt intake during the prehypertensive period in hypertensive model animals. Dahl salt-sensitive rats and spontaneously hypertensive rats were fed from 6 to 14 weeks with low-salt (0.12% NaCl), normal-salt (0.8% NaCl), high-salt (7% NaCl), or high-sodium/normal-chloride diet and returned to normal-salt diet for 3 months. Rats in the high-salt group saw elevations in blood pressure (BP) not only during the treatment period but also for the 3 months after returning to normal-salt diet. We named this phenomenon salt memory. Renal arteriolar injury was found in the high-salt group at the end of experiment. Dahl salt-sensitive rats were fed from 6 to 14 weeks with high-salt diet with angiotensin receptor blocker, vasodilator, calcium channel blocker, and calcium channel blocker+angiotensin receptor blocker and returned to normal-salt diet. Although BP was suppressed to control levels by vasodilator or calcium channel blocker, elevated renal angiotensin II and renal arteriolar injury were observed, and salt memory did not disappear because of sustained renal arteriolar injury. Calcium channel blocker+angiotensin receptor blocker suppressed renal arteriolar injury, resulting in the disappearance of salt memory. Cross-transplantation of kidneys from Dahl salt-sensitive rats on high salt to control rats caused increase of BP, whereas control kidneys caused reduction in BP of hypertensive rats, inducing the central role of the kidney. These results suggest that renal arteriolar injury through BP and renal angiotensin II elevation plays important roles in the development of salt memory for hypertension.
Assuntos
Arteríolas/fisiopatologia , Pressão Sanguínea/fisiologia , Hipertensão/fisiopatologia , Rim/irrigação sanguínea , Cloreto de Sódio na Dieta/administração & dosagem , Angiotensina II/sangue , Angiotensina II/metabolismo , Antagonistas de Receptores de Angiotensina/farmacologia , Animais , Arteríolas/efeitos dos fármacos , Arteríolas/lesões , Pressão Sanguínea/efeitos dos fármacos , Western Blotting , Bloqueadores dos Canais de Cálcio/farmacologia , Canais Epiteliais de Sódio/genética , Canais Epiteliais de Sódio/metabolismo , Expressão Gênica/efeitos dos fármacos , Hipertensão/etiologia , Glomérulos Renais/efeitos dos fármacos , Glomérulos Renais/patologia , Glomérulos Renais/fisiopatologia , Transplante de Rim/métodos , Masculino , Ratos , Ratos Endogâmicos Dahl , Ratos Endogâmicos SHR , Renina/sangue , Sistema Renina-Angiotensina/efeitos dos fármacos , Sistema Renina-Angiotensina/fisiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Cloreto de Sódio na Dieta/toxicidade , Fatores de TempoRESUMO
The mouse laser injury thrombosis model provides up to 0.22 µm-resolved voxel information about the pore architecture of the dense inner core and loose outer shell regions of an in vivo arterial thrombus. Computational studies were conducted on this 3D structure to quantify transport within and around the clot: Lattice Boltzmann method defined vessel hemodynamics, while passive Lagrangian Scalar Tracking with Brownian motion contribution simulated diffusive-convective transport of various inert solutes (released from lumen or the injured wall). For an input average lumen blood velocity of 0.478 cm/s (measured by Doppler velocimetry), a 0.2 mm/s mean flow rate was obtained within the thrombus structure, most of which occurred in the 100-fold more permeable outer shell region (calculated permeability of the inner core was 10(-11) cm(2)). Average wall shear stresses were 80-100 dyne/cm(2) (peak values >200 dyne/cm(2)) on the outer rough surface of the thrombus. Within the thrombus, small molecule tracers (0.1 kDa) experienced ~70,000 collisions/s and penetrated/exited it in about 1 s, whereas proteins (~50 kDa) had ~9000 collisions/s and required about 10 s (tortuosity ~2-2.5). These simulations help define physical processes during thrombosis and constraints for drug delivery to the thrombus.
Assuntos
Arteríolas/fisiopatologia , Modelos Biológicos , Trombose/fisiopatologia , Animais , Arteríolas/lesões , Plaquetas/fisiologia , Simulação por Computador , Análise de Elementos Finitos , Fluxometria por Laser-Doppler , Lasers , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/irrigação sanguínea , Músculo Esquelético/lesões , Porosidade , Fluxo Sanguíneo RegionalRESUMO
OBJECTIVE: Platelet granule exocytosis serves a central role in hemostasis and thrombosis. Recently, single-cell amperometry has shown that platelet membrane fusion during granule exocytosis results in the formation of a fusion pore that subsequently expands to enable the extrusion of granule contents. However, the molecular mechanisms that control platelet fusion pore expansion and collapse are not known. METHODS AND RESULTS: We identified dynamin-related protein-1 (Drp1) in platelets and found that an inhibitor of Drp1, mdivi-1, blocked exocytosis of both platelet dense and α-granules. We used single-cell amperometry to monitor serotonin release from individual dense granules and, thereby, measured the effect of Drp1 inhibition on fusion pore dynamics. Inhibition of Drp1 increased spike width and decreased prespike foot events, indicating that Drp1 influences fusion pore formation and expansion. Platelet-mediated thrombus formation in vivo after laser-induced injury of mouse cremaster arterioles was impaired after infusion of mdivi-1. CONCLUSIONS: These results demonstrate that inhibition of Drp1 disrupts platelet fusion pore dynamics and indicate that Drp1 can be targeted to control thrombus formation in vivo.
Assuntos
Plaquetas/metabolismo , Dinaminas/sangue , Exocitose , GTP Fosfo-Hidrolases/sangue , Fusão de Membrana , Proteínas Associadas aos Microtúbulos/sangue , Proteínas Mitocondriais/sangue , Vesículas Secretórias/metabolismo , Trombose/sangue , Lesões do Sistema Vascular/sangue , Animais , Arteríolas/lesões , Plaquetas/efeitos dos fármacos , Modelos Animais de Doenças , Dinaminas/antagonistas & inibidores , Exocitose/efeitos dos fármacos , Fibrinolíticos/farmacologia , GTP Fosfo-Hidrolases/antagonistas & inibidores , Humanos , Lasers , Fusão de Membrana/efeitos dos fármacos , Camundongos , Proteínas Associadas aos Microtúbulos/antagonistas & inibidores , Proteínas Mitocondriais/antagonistas & inibidores , Selectina-P/sangue , Quinazolinonas/farmacologia , Coelhos , Vesículas Secretórias/efeitos dos fármacos , Serotonina/sangue , Trombose/etiologia , Trombose/prevenção & controle , Fatores de Tempo , Lesões do Sistema Vascular/etiologiaRESUMO
Extracellular protein disulfide isomerase (PDI) is required for platelet thrombus formation and fibrin generation after arteriolar wall injury in live mice. PDI is secreted from platelets and endothelial cells on cellular activation, but the mechanism of capture of secreted PDI within the injured vasculature is unknown. We establish that, like the endothelial ß3 integrin α(V)ß(3), the platelet integrin α(IIb)ß(3) binds PDI. PDI also binds to recombinant ß3. Using intravital microscopy, we demonstrate that PDI accumulation at the site of laser-induced arteriolar wall injury is markedly reduced in ß3-null (ß3(-/-)) mice, and neither a platelet thrombus nor fibrin is generated at the vessel injury site. The absence of fibrin after vascular injury in ß3(-/-) mice is because of the absence of extracellular PDI. To evaluate the relative importance of endothelial α(V)ß(3) versus platelet α(IIb)ß(3) or α(V)ß(3), we performed reciprocal bone marrow transplants on wild-type and ß3(-/-) mice. PDI accumulation and platelet thrombus formation were markedly decreased after vessel injury in wild-type mice transplanted with ß3(-/-) bone marrow or in ß3(-/-) mice transplanted with wild-type bone marrow. These results indicate that both endothelial and platelet ß3 integrins contribute to extracellular PDI binding at the vascular injury site.
Assuntos
Plaquetas/enzimologia , Células Endoteliais/enzimologia , Integrina beta3/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo , Trombose/metabolismo , Animais , Arteríolas/enzimologia , Arteríolas/lesões , Coagulação Sanguínea/fisiologia , Plaquetas/metabolismo , Transplante de Medula Óssea , Células CHO , Cricetinae , Espaço Extracelular/enzimologia , Integrina alfaVbeta3/metabolismo , Camundongos , Camundongos da Linhagem 129 , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Microscopia de Vídeo , Músculo Esquelético/irrigação sanguínea , Complexo Glicoproteico GPIIb-IIIa de Plaquetas/metabolismo , Quimeras de TransplanteRESUMO
OBJECTIVE: To evaluate the feasibility and safety of endovascular treatment of ruptured and unruptured intracranial very small aneurysms (< or = 3 mm in maximal diameter). METHODS: Forty-eight intracranial very small aneurysms in 44 patients treated with endovascular therapy from June 2001 to August 2009 were reviewed retrospectively in clinical, imaging, interventional and follow-up data. Among 44 patients, there were 20 males and 24 females with a mean age of 57.8 years old. The Hunt-Kosnik grade was as follows: Grade 0 (n = 11); Grades I & II (n = 23); Grades III & IV (n = 9); and ungraded (n = 1). The sizes of 48 aneurysms were not more than 3 mm in maximal diameter. The locations of aneurysms were as follows: ACoA (n = 11), MCA (n = 8), PCoA (n = 14), ICA (n = 12), pericallosal artery (n = 1), VA (n = 1) and PICA (n = 1). Thirty-nine aneurysms were embolized with coil, of which 13 with stent assistance and 6 by balloon remodeling technique. The other 9 aneurysms underwent sole stent placement in parent artery. RESULTS: Among 39 coiling aneurysms, 100% occlusion was achieved in 9 aneurysms, 90% in 20, 80% in 9 and less than 80% in 1 respectively. Only one aneurysm ruptured during coiling. Two patients had transient hemiparesis and one patient had ataxia caused by bilateral cerebellar infarction postoperatively. All patients were clinically followed up for 4-90 months and no recurrent hemorrhage occurred. Thirteen patients received repeat angiography at 4-72 months post-treatment. And no radiological re-growth was detected. CONCLUSION: Endovascular treatment of ruptured and unruptured intracranial very small aneurysms seems to be technically feasible, relatively safe and practically effective.
Assuntos
Arteríolas/lesões , Embolização Terapêutica/métodos , Aneurisma Intracraniano/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Aneurisma Intracraniano/patologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Ruptura , Resultado do TratamentoRESUMO
This study examines mechanisms by which changes in tissue oxygen tension elicit vasomotor responses and whether localized changes in oxygen tension initiates conducted vasomotor responses in mouse cremaster arterioles. Intravital microscopy was used to visualize the mouse cremaster microcirculation. The cremaster was superfused with Krebs' solution with different oxygen tensions, and a gas exchange chamber was used to induce localized changes in oxygen tension. In arterioles where red blood cells were removed by buffer perfusion, arterioles responded with same magnitudes of vasodilatation (DeltaD = 16.0 +/- 4.9 microm) when changing from high (PO(2) = 242.5 +/- 13.3 mm Hg) to low (PO(2) = 22.5 +/- 4.8 mm Hg) oxygen tension as seen in the intact cremaster circulation (DeltaD = 18.7 +/- 1.0 microm). Blockade of NO synthases by L: -NAME and adenosine receptors by DPCPX had no effects on vasomotor responses to low or high oxygen. Induction of localized low (PO(2) = 23.3 +/- 5.7 mmHg) or high (PO(2) = 300.0 +/- 25.7 mm Hg) oxygen tension caused vasodilatation or -constriction locally and at a site 1,000 microm upstream (distantly). Glibenclamide blocker of ATP-sensitive K(+) channels inhibited vasodilatation and -constriction to low (PO(2) = 16.0 +/- 6.4 mm Hg) and high (PO(2) = 337.4 +/- 12.8 mm Hg) oxygen tension. 1) ATP-sensitive K(+) channels seem to mediate, at least in part, vasodilatation and vasoconstriction to low and high oxygen tension; 2) Red blood cells are not necessary for inducing vasodilatation and vasoconstriction to low or high oxygen tension; 3) localized changes in the oxygen tension cause vasomotor responses, which are conducted upstream along arterioles in mouse cremaster microcirculation.
Assuntos
Músculos/irrigação sanguínea , Oxigênio/metabolismo , Vasoconstrição , Vasodilatação , Adenosina/metabolismo , Antagonistas do Receptor A1 de Adenosina , Antagonistas do Receptor A2 de Adenosina , Animais , Arteríolas/lesões , Arteríolas/metabolismo , Hipóxia Celular , Inibidores Enzimáticos/farmacologia , Eritrócitos/metabolismo , Canais KATP/antagonistas & inibidores , Canais KATP/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microcirculação , Microscopia de Vídeo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico Sintase/metabolismo , Oxigênio/sangue , Perfusão , Bloqueadores dos Canais de Potássio/farmacologia , Receptor A1 de Adenosina/metabolismo , Receptores A2 de Adenosina/metabolismo , Fluxo Sanguíneo Regional , Vasoconstrição/efeitos dos fármacos , Vasodilatação/efeitos dos fármacosRESUMO
Fibrin is an integral component of arterial thrombi. Using a mouse model of arteriolar thrombosis, high-speed fluorescence microscopy reveals that, within minutes, the fibrin content of thrombi rapidly increases and then decreases. The decrease in fibrin coincides with leukocyte binding to the thrombi, a process mediated by the interaction of leukocyte P-selectin glycoprotein ligand-1 (PSGL-1) with P-selectin on the surface of activated platelets. Because leukocytes possess urokinase-type plasminogen activator (uPA) activity, we used mice deficient in uPA or the uPA receptor (uPAR) to explore the contribution of leukocyte-associated uPA to the loss of fibrin from these thrombi. Fibrin loss in both uPA-deficient mice and uPAR-deficient mice was reduced compared with that in wild-type controls. Transfusion of leukocytes from wild-type mice into uPAR-deficient mice restored fibrin loss to levels similar to that in wild-type mice. In contrast, transfusion of leukocytes from mice deficient in uPAR or PSGL-1 did not enhance fibrin loss. Thus, fibrin loss from microarteriolar thrombi is mediated, at least in part, by leukocyte-associated uPA in a process that requires leukocyte uPAR and PSGL-1.
Assuntos
Fibrinólise/fisiologia , Leucócitos/fisiologia , Glicoproteínas de Membrana/sangue , Receptores de Ativador de Plasminogênio Tipo Uroquinase/sangue , Ácido Aminocaproico/farmacologia , Animais , Arteríolas/lesões , Arteríolas/fisiopatologia , Plaquetas/patologia , Modelos Animais de Doenças , Fibrina/metabolismo , Fibrinólise/genética , Transfusão de Leucócitos , Glicoproteínas de Membrana/deficiência , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptores de Ativador de Plasminogênio Tipo Uroquinase/deficiência , Receptores de Ativador de Plasminogênio Tipo Uroquinase/genética , Trombose/sangue , Trombose/etiologia , Trombose/fisiopatologia , Ativador de Plasminogênio Tipo Uroquinase/sangue , Ativador de Plasminogênio Tipo Uroquinase/deficiência , Ativador de Plasminogênio Tipo Uroquinase/genéticaRESUMO
AIM: To investigate microvascular injury quantitatively in the small bowel with respect to cardiopulmonary bypass (CPB) and related mechanisms. METHODS: In 10 male SD rats, normothermic CPB was established and continued with a flow rate of 100-150 mL/kg per minute for 60 min, while another 10 sham-operated animals served as controls. An approximate 10-cm loop of the terminal ileum was exteriorized for observation by means of intravital fluorescence microscopy. The small bowel microcirculatory network including arterioles, capillaries, and collecting venules was observed prior to CPB, CPB 30 min, CPB 60 min, post-CPB 60 min and post-CPB 120 min. The intestinal capillary perfusion, microvascular permeability and leukocyte adherence were also measured. RESULTS: The systemic hemodynamics remained stable throughout the experiment in both groups. In CPB animals, significant arteriolar vasoconstriction, blood velocity reduction and functional capillary density diminution were found. As concomitances, exaggerated albumin extravasation and increased leukocyte accumulation were also noted. These changes were more pronounced and there were no signs of restitution at the end of the observation period. CONCLUSION: CPB induces significant microcirculatory injury of the small bowel in rats. The major underlying mechanisms are blood flow redistribution and generalized inflammatory response associated with CPB.
Assuntos
Capilares/lesões , Ponte Cardiopulmonar/efeitos adversos , Intestino Delgado , Microcirculação , Animais , Arteríolas/anatomia & histologia , Arteríolas/lesões , Capilares/anatomia & histologia , Permeabilidade Capilar , Hemodinâmica , Humanos , Intestino Delgado/irrigação sanguínea , Intestino Delgado/patologia , Leucócitos/metabolismo , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Fluxo Sanguíneo RegionalRESUMO
Individuals whose platelets lack dense or alpha-granules suffer various degrees of abnormal bleeding, implying that granule cargo contributes to hemostasis. Despite these clinical observations, little is known regarding the effects of impaired platelet granule secretion on thrombus formation in vivo. In platelets, SNARE proteins mediate the membrane fusion events required for granule cargo release. Endobrevin/VAMP-8 is the primary vesicle-SNARE (v-SNARE) responsible for efficient release of dense and alpha-granule contents; thus, VAMP-8(-/-) mice are a useful model to evaluate the importance of platelet granule secretion in thrombus formation. Thrombus formation, after laser-induced vascular injury, in these mice is delayed and decreased, but not absent. In contrast, thrombus formation is almost completely abolished in the mouse model of Hermansky-Pudlak syndrome, ruby-eye, which lacks dense granules. Evaluation of aggregation of VAMP-8(-/-) and ruby-eye platelets indicates that defective ADP release is the primary abnormality leading to impaired aggregation. These results demonstrate the importance of dense granule release even in the earliest phases of thrombus formation and validate the distal platelet secretory machinery as a potential target for antiplatelet therapies.
Assuntos
Transtornos da Coagulação Sanguínea/sangue , Coagulação Sanguínea/fisiologia , Plaquetas/metabolismo , Grânulos Citoplasmáticos/metabolismo , Agregação Plaquetária/fisiologia , Proteínas R-SNARE/fisiologia , Difosfato de Adenosina/metabolismo , Animais , Arteríolas/lesões , Transtornos da Coagulação Sanguínea/etiologia , Plaquetas/química , Plaquetas/ultraestrutura , Modelos Animais de Doenças , Síndrome de Hermanski-Pudlak/sangue , Humanos , Lasers/efeitos adversos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Mutantes , Proteínas R-SNARE/deficiência , Proteínas R-SNARE/genética , Proteínas SNARE/sangueRESUMO
PURPOSE: Shockwave lithotripsy (SWL) injures renal tissue, and cavitation has been reported to mediate some of these effects. Much of the work characterizing the cavitation injury of SWL has been performed in small animals or in vitro. We describe experiments that promote cavitation during SWL and estimate the spatial distribution of the resulting hemorrhagic lesion in a large-animal (porcine) model of clinical lithotripsy. MATERIALS AND METHODS: The lower pole calix of the left kidney in female farm pigs was targeted for SWL with a Dornier HM3 lithotripter. Intraventricular injections of polystyrene microspheres were made before and at intervals during lithotripsy to blanket systemic circulation with cavitation nuclei. Following SWL, the abdominal viscera were inspected and the kidneys were processed for morphologic analysis. RESULTS: Extensive surface hemorrhage occurred over both the targeted and contralateral kidneys, along with widespread petechial hemorrhage over the spleen, intestines, and peritoneum. The targeted kidneys developed subcapsular hematomas. Histology revealed focal and diffuse damage to the targeted kidneys and vascular rupture in both kidneys with complete necrosis of the walls of intralobular arteries and veins. CONCLUSIONS: These results demonstrate the potential for unfocused shockwaves to damage blood vessels outside the focal zone of the lithotripter when the vasculature is seeded with cavitation nuclei. The wide distribution of damage suggests that the acoustic field of a lithotripter delivers negative pressures that exceed the cavitation threshold far off the acoustic axis. The findings underscore that conditions permissive for cavitation can lead to dramatic sequelae during SWL.
Assuntos
Rim/lesões , Litotripsia/efeitos adversos , Animais , Arteríolas/lesões , Arteríolas/patologia , Feminino , Hemorragia/etiologia , Rim/irrigação sanguínea , Rim/patologia , Sus scrofaRESUMO
The metalloprotease ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type I repeats 13) cleaves highly adhesive large von Willebrand factor (VWF) multimers after their release from the endothelium. ADAMTS13 deficiency is linked to a life-threatening disorder, thrombotic thrombocytopenic purpura (TTP), characterized by platelet-rich thrombi in the microvasculature. Here, we show spontaneous thrombus formation in activated microvenules of Adamts13-/- mice by intravital microscopy. Strikingly, we found that ADAMTS13 down-regulates both platelet adhesion to exposed subendothelium and thrombus formation in injured arterioles. An inhibitory antibody to ADAMTS13 infused in wild-type mice prolonged adhesion of platelets to endothelium and induced thrombi formation with embolization in the activated microvenules. Absence of ADAMTS13 did not promote thrombi formation in alphaIIbbeta3 integrin-inhibited blood. Recombinant ADAMTS13 reduced platelet adhesion and aggregation in histamine-activated venules and promoted thrombus dissolution in injured arterioles. Our findings reveal that ADAMTS13 has a powerful natural antithrombotic activity and recombinant ADAMTS13 could be used as an antithrombotic agent.