Expression analysis of light-regulated genes isolated from a full-length-enriched cDNA library of Onosma paniculatum cell cultures.

Shikonin and its derivatives are formed in large amounts in dark-cultured Onosma paniculatum cells. In order to isolate and identify the genes regulating shikonin biosynthesis, we constructed and characterized a full-length-enriched cDNA library of dark-cultured cells by using the SMART (Switching Mechanism At 5'-end of RNA Transcript) cDNA synthesis and LD-PCR (long-distance PCR) strategies. The titer of the primary cDNA library was 1.04 x 10(6)pfu/mL with a recombination rate of 99.60%. Most of the cDNA inserts ranged from 1.0 to 2.5 kb, and 78.33% of the 76 randomly selected clones contained full-length coding regions. Expression analysis of randomly selected genes by small scale microarray revealed that 23 genes were down-regulated, including 17 genes with known functions, 2 genes with putative functions, and 4 novel genes, and that 3 genes were up-regulated (two-fold) in cells cultured under white light as compared with those cultured in the dark. Interestingly, two of the down-regulated genes, encoding aci-reductone dioxygenase (ARD)-like protein and ethylene responsive factor (ERF), are involved in ethylene biosynthesis and signal transduction, implying that ethylene might play an important role as a signal molecule in light-regulated shikonin formation. These data contribute to a better understanding of light-involvement in regulating the formation of plant secondary metabolites.

[Characteristics of natural radionuclide distribution in different organs of plants growing on territory with increased radiation background].

We investigated wild-growing grassy plants such as Alhagi pseudalhagi, Zygophyllum, Juncus acutus and Argusia sibirica with the purpose of studying the mechanism of carry of radionuclides from ground in plants and an establishment most the common laws of distribution of radionuclides between different organs of plants. The results of the research show that the researched plants are mainly accumulated 40K. On a degree of accumulation, 226Ra occupies the second place, but 232Th doesn't participate almost in processes of carry from ground in plants. It is established that root systems of plants possesses unequal ability to absorb same radionuclides from the same ground. For example, the root system of Argusia sibirica is characterized by smaller ability to absorb 226Ra, but 40K is more accessible. It is shown that biological availability radionuclides in the given ground depends both on a kind of plants and on radionuclides. For example, Argusia sibirica, Zygophyllum show higher accumulating ability to 40K and in relation to 226Ra, Alhagi pseudalhagi and Juncus acutus are more sensitive. It is established that accumulating ability of stalks of different plants in relation to 40K are appreciably different. Distribution of radionuclides in seeds of plants has other character. Thus Alhagi pseudalhagi in the seeds had also certain amount of 232Th, and leaves Argusia sibirica in comparison with Zygophyllum have a high degree of accumulation as for 40K, so for 226Ra.