RESUMO
Intracellular pathogens are challenged with limited space and resources while replicating in a single host cell. Mechanisms for direct invasion of neighboring host cells have been discovered in cell culture, but we lack an understanding of how bacteria directly spread between host cells in vivo. Here, we describe the discovery of intracellular bacteria that use filamentation for spreading between the intestinal epithelial cells of a natural host, the rhabditid nematode Oscheius tipulae. The bacteria, which belong to the new species Bordetella atropi, can infect the nematodes following a fecal-oral route, and reduce host life span and fecundity. Filamentation requires UDP-glucose biosynthesis and sensing, a highly conserved pathway that is used by other bacteria to detect rich conditions and inhibit cell division. Our results indicate that B. atropi uses a pathway that normally regulates bacterial cell size to trigger filamentation inside host cells, thus facilitating cell-to-cell dissemination.
Assuntos
Bordetella/crescimento & desenvolvimento , Mucosa Intestinal/citologia , Rhabditoidea/citologia , Animais , Bordetella/classificação , Bordetella/patogenicidade , Divisão Celular/genética , Células Epiteliais/microbiologia , Células Epiteliais/ultraestrutura , Genoma Bacteriano/genética , Interações Hospedeiro-Patógeno , Hibridização in Situ Fluorescente , Mucosa Intestinal/microbiologia , Espaço Intracelular/microbiologia , Redes e Vias Metabólicas/genética , Microscopia Eletrônica de Transmissão , Filogenia , RNA Ribossômico 16S/genética , Rhabditoidea/genética , Rhabditoidea/microbiologia , Análise de Sequência de DNA , VirulênciaRESUMO
PURPOSE: The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. METHODS: This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. RESULTS: Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy control. CONCLUSION: This study supports the usefulness of 16S rRNA gene sequencing for improved sensitivity on pertussis diagnosis compared to real-time PCR and to understand other microbial changes occurring in the nasopharynx in children <1 year old with suspected whooping cough compared to healthy controls.
Assuntos
Microbiota , Coqueluche/microbiologia , Bordetella/genética , Bordetella/isolamento & purificação , Bordetella/patogenicidade , Estudos de Casos e Controles , Feminino , Humanos , Lactente , Masculino , Cavidade Nasal/microbiologia , Faringe/microbiologia , RNA Ribossômico 16S/genética , Coqueluche/diagnósticoRESUMO
The lungs possess an effective antimicrobial system and a strong ability to eliminate microorganisms in healthy organisms, and were once considered sterile. With the development of culture-independent sequencing technology, the richness and diversity of porcine lung microbiota have been gaining attention. In order to study the relationship between lung microbiota and porcine respiratory disease complex (PRDC), the lung microbiota in healthy and diseased swine bronchoalveolar lavage fluids were analyzed and compared using the Illumina MiSeq sequencing platform. The predominant microbial communities of healthy and diseased swine were similar at the phylum level, mainly composed of Proteobacteria, Firmicutes, Tenericutes, and Bacteroidetes. However, the bacterial taxonomic communities of healthy and diseased swine differed at the genus level. The higher relative abundances of Lactococcus, Enterococcus, Staphylococcus, and Lactobacillus genera in healthy swine might provide more benefits for lung health, while the enhanced richness of Streptococcus, Haemophilus, Pasteurella, and Bordetella genera in diseased swine might be closely related to pathogen invasion and the occurrence of respiratory disease. In conclusion, the observed differences in the richness and diversity of lung microbiota can provide novel insights into their relationship with PRDC. Analyses of swine lung microbiota communities might produce an effective strategy for the control and prevention of respiratory tract infections.
Assuntos
DNA Bacteriano/genética , Pulmão/microbiologia , Microbiota/genética , Infecções Respiratórias/microbiologia , Suínos/microbiologia , Animais , Bordetella/classificação , Bordetella/genética , Bordetella/isolamento & purificação , Bordetella/patogenicidade , Líquido da Lavagem Broncoalveolar/microbiologia , Enterococcus/classificação , Enterococcus/genética , Enterococcus/isolamento & purificação , Haemophilus/classificação , Haemophilus/genética , Haemophilus/isolamento & purificação , Haemophilus/patogenicidade , Sequenciamento de Nucleotídeos em Larga Escala , Lactobacillus/classificação , Lactobacillus/genética , Lactobacillus/isolamento & purificação , Lactococcus/classificação , Lactococcus/genética , Lactococcus/isolamento & purificação , Pasteurella/classificação , Pasteurella/genética , Pasteurella/isolamento & purificação , Pasteurella/patogenicidade , Filogenia , RNA Ribossômico 16S/genética , Staphylococcus/classificação , Staphylococcus/genética , Staphylococcus/isolamento & purificação , Streptococcus/classificação , Streptococcus/genética , Streptococcus/isolamento & purificação , Streptococcus/patogenicidadeRESUMO
Infection and inflammation of the middle ears that characterizes acute and chronic otitis media (OM), is a major reason for doctor visits and antibiotic prescription, particularly among children. Nasopharyngeal pathogens that are commonly associated with OM in humans do not naturally colonize the middle ears of rodents, and experimental models in most cases involve directly injecting large numbers of human pathogens into the middle ear bullae of rodents, where they induce a short-lived acute inflammation but fail to persist. Here we report that Bordetella pseudohinzii, a respiratory pathogen of mice, naturally, efficiently and rapidly ascends the eustachian tubes to colonize the middle ears, causing acute and chronic histopathological changes with progressive decrease in hearing acuity that closely mimics otitis media in humans. Laboratory mice experimentally inoculated intranasally with very low numbers of bacteria consistently have their middle ears colonized and subsequently transmit the bacterium to cage mates. Taking advantage of the specifically engineered and well characterized immune deficiencies available in mice we conducted experiments to uncover different roles of T and B cells in controlling bacterial numbers in the middle ear during chronic OM. The iconic mouse model provides significant advantages for elucidating aspects of host-pathogen interactions in otitis media that are currently not possible using other animal models. This natural model of otitis media permits the study of transmission between hosts, efficient early colonization of the respiratory tract, ascension of the eustachian tube, as well as colonization, pathogenesis and persistence in the middle ear. It also allows the combination of the powerful tools of mouse molecular immunology and bacterial genetics to determine the mechanistic basis for these important processes.
Assuntos
Infecções por Bordetella/transmissão , Bordetella/patogenicidade , Modelos Animais de Doenças , Tuba Auditiva/microbiologia , Cavidade Nasal/microbiologia , Otite Média/microbiologia , Animais , Infecções por Bordetella/complicações , Infecções por Bordetella/microbiologia , Doença Crônica , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BLRESUMO
Bacteria use a variety of mechanisms to translocate proteins from the cytoplasm, where they are synthesized, to the cell surface or extracellular environment or directly into other cells, where they perform their ultimate functions. Type V secretion systems (T5SS) use ß-barrel transporter domains to export passenger domains across the outer membranes of Gram-negative bacteria. Distinct among T5SS are type Vb or two-partner secretion (TPS) systems in which the transporter and passenger are separate proteins, necessitating a mechanism for passenger-translocator recognition in the periplasm and providing the potential for reuse of the translocator. This review describes current knowledge of the TPS translocation mechanism, using Bordetella filamentous hemagglutinin (FHA) and its transporter FhaC as a model. We present the hypothesis that the TPS pathway may be a general mechanism for contact-dependent delivery of toxins to target cells.
Assuntos
Bordetella/metabolismo , Hemaglutininas/metabolismo , Via Secretória/fisiologia , Adesinas Bacterianas/metabolismo , Proteínas da Membrana Bacteriana Externa/metabolismo , Bordetella/patogenicidade , Bordetella pertussis/metabolismo , Bordetella pertussis/patogenicidade , Bactérias Gram-Negativas , Proteínas de Membrana Transportadoras , Modelos Moleculares , Sistemas de Secreção Tipo V/metabolismo , Virulência , Fatores de Virulência de Bordetella/metabolismo , Coqueluche/microbiologiaRESUMO
Several species of the Gram-negative genus Bordetella are the cause of respiratory infections in mammals and birds, including whooping cough (pertussis) in humans. Very recently, a novel atypical species, Bordetella pseudohinzii, was isolated from laboratory mice. These mice presented no obvious clinical symptoms but elevated numbers of neutrophils in bronchoalveolar lavage fluid and inflammatory signs in histopathology. We noted that this species can occur at high prevalence in a mouse facility despite regular pathogen testing according to the FELASA-recommendations. Affected C57BL/6 J mice had, in addition to the reported pulmonary alterations, tracheal inflammation with reduced numbers of ciliated cells, slower ciliary beat frequency, and largely (>50%) compromised cilia-driven particle transport speed on the mucosal surface, a primary innate defence mechanism. In an in vitro-model, Bordetella pseudohinzii attached to respiratory kinocilia, impaired ciliary function within 4 h and caused epithelial damage within 24 h. Regular testing for this ciliotropic Bordetella species and excluding it from colonies that provide mice for lung research shall be recommended. On the other hand, controlled colonization and infection with Bordetella pseudohinzii may serve as an experimental model to investigate mechanisms of mucociliary clearance and microbial strategies to escape from this primary innate defence response.
Assuntos
Infecções por Bordetella/veterinária , Bordetella/fisiologia , Infecções Respiratórias/veterinária , Doenças dos Roedores/microbiologia , Traqueia/microbiologia , Animais , Bordetella/classificação , Bordetella/isolamento & purificação , Bordetella/patogenicidade , Infecções por Bordetella/epidemiologia , Infecções por Bordetella/microbiologia , Cílios/microbiologia , DNA Bacteriano/análise , Camundongos , Camundongos Endogâmicos C57BL , Depuração Mucociliar , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Análise de Sequência de DNA , Traqueia/metabolismo , Traqueia/patologiaRESUMO
The genus Bordetella comprises several bacterial species that colonize the respiratory tract of mammals. It includes B. pertussis, a human-restricted pathogen that is the causative agent of Whooping Cough. In contrast, the closely related species B. bronchiseptica colonizes a broad range of animals as well as immunocompromised humans. Recent metagenomic studies have identified known and novel bordetellae isolated from different environmental sources, providing a new perspective on their natural history. Using phylogenetic analysis, we have shown that human and animal pathogenic bordetellae have most likely evolved from ancestors that originated from soil and water. Our recent study found that B. bronchiseptica can evade amoebic predation and utilize Dictyostelium discoideum as an expansion and transmission vector, which suggests that the evolutionary pressure to evade the amoebic predator enabled the rise of bordetellae as respiratory pathogens. Interactions with amoeba may represent the starting point for bacterial adaptation to eukaryotic cells. However, as bacteria evolve and adapt to a novel host, they can become specialized and restricted to a specific host. B. pertussis is known to colonize and cause infection only in humans, and this specialization to a closed human-to-human lifecycle has involved genome reduction and the loss of ability to utilize amoeba as an environmental reservoir. The discoveries from studying the interaction of Bordetella species with amoeba will elicit a better understanding of the evolutionary history of these and other important human pathogens.
Assuntos
Adaptação Biológica , Infecções por Bordetella/microbiologia , Bordetella/genética , Bordetella/patogenicidade , Dictyostelium/microbiologia , Microbiologia Ambiental , Infecções Respiratórias/microbiologia , Animais , Bordetella/classificação , Infecções por Bordetella/veterinária , Humanos , Filogenia , Infecções Respiratórias/veterináriaRESUMO
Since the first description of Bordetella holmesii in 1995, almost 100 publications have contributed to the increasing knowledge of this emerging bacterium. Although first reported to induce bacteremia mainly in immunocompromised patients, it has also been isolated in healthy persons and has shown the capacity to induce pertussis-like symptoms and other clinical entities, such as meningitis, arthritis, or endocarditis. Respiratory diseases are generally less severe than those induced by Bordetella pertussis. However, B. holmesii was found to have a higher capacity of invasiveness given the various infection sites in which it was isolated. The diagnosis is difficult, particularly as it is a slow-growing organism but also because respiratory infections are systematically misdiagnosed as B. pertussis. Treatment is delicate, as its susceptibility to macrolides (prescribed in respiratory infections) and ceftriaxone (used in invasive disease) is challenged. Regarding prevention, there is no consensus on prophylactic treatment following index cases and no vaccine is available. Epidemiological data are also sparse, with few prevalence studies available. In this chapter, we provide an overview of the current state of knowledge on B. holmesii.
Assuntos
Infecções por Bordetella/microbiologia , Bordetella/fisiologia , Bordetella/efeitos dos fármacos , Bordetella/patogenicidade , Infecções por Bordetella/diagnóstico , Infecções por Bordetella/epidemiologia , Infecções por Bordetella/terapia , Ceftriaxona/uso terapêutico , Humanos , Macrolídeos/uso terapêuticoRESUMO
The Bordetella genus comprises nine species of which Bordetella pertussis and B. parapertussis are isolated from humans and are the most studied Bordetella species since they cause whooping cough. They both originate from B. bronchiseptica, which infects several mammals and immune compromised humans, but the intensive use of pertussis vaccines induced changes in B. pertussis and B. parapertussis populations. B. petrii and B. holmesii are other species of unknown reservoir and transmission pattern that have been described in humans. It is still unknown whether these species are pathogens for humans or only opportunistic bacteria but biological diagnosis has confirmed the presence of B. holmesii in human respiratory samples while B. petrii and the four other species have little implications for public health.
Assuntos
Infecções por Bordetella/epidemiologia , Infecções por Bordetella/microbiologia , Bordetella/imunologia , Bordetella/isolamento & purificação , Vacina contra Coqueluche/imunologia , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/microbiologia , Bordetella/patogenicidade , Infecções por Bordetella/prevenção & controle , Humanos , Vacina contra Coqueluche/administração & dosagem , Infecções Respiratórias/prevenção & controleRESUMO
Bordetella holmesii, first described in 1995, is believed to cause both invasive infections (bacteraemia, meningitis, endocarditis, pericarditis, pneumonia, and arthritis) and pertussis-like symptoms. Infection with B holmesii is frequently misidentified as being with B pertussis, the cause of whooping cough, because routine diagnostic tests for pertussis are not species-specific. In this Review, we summarise knowledge about B holmesii diagnosis and treatment, and assess research needs. Although no fatal cases of B holmesii have been reported, associated invasive infections can cause substantial morbidities, even in previously healthy individuals. Antimicrobial treatment can be problematic because B holmesii's susceptibility to macrolides (used empirically to treat B pertussis) and third-generation cephalosporins (often used to treat invasive infections) is lower than would be expected. B holmesii's adaptation to human beings is continuing, and virulence might increase, causing the need for better diagnostic assays and epidemiological surveillance.
Assuntos
Bacteriemia/microbiologia , Infecções por Bordetella/microbiologia , Bordetella/isolamento & purificação , Genoma Bacteriano/genética , Bacteriemia/diagnóstico , Bacteriemia/epidemiologia , Bacteriemia/prevenção & controle , Bordetella/genética , Bordetella/patogenicidade , Infecções por Bordetella/diagnóstico , Infecções por Bordetella/epidemiologia , Infecções por Bordetella/prevenção & controle , Diagnóstico Diferencial , Humanos , Incidência , Testes de Sensibilidade Microbiana , Prevalência , Especificidade da Espécie , VirulênciaRESUMO
Bordetella is a Gram-negative bacterium responsible for causing whooping cough in a broad range of host organisms. For successful infection, Bordetella controls expression of four distinct classes of genes (referred to as class 1, 2, 3, and 4 genes) at distinct times in the infection cycle. This control is executed by a single two-component system, BvgAS. Interestingly, the transmembrane component of the two-component system, BvgS, consists of three phospho-transfer domains leading to phosphorylation of the response regulator, BvgA. Phosphorylated BvgA then controls expression of virulence genes and also controls bvgAS transcription. In this work, we perform simulations to characterize the role of the network architecture in governing gene expression in Bordetella. Our results show that the wild-type network is locally optimal for controlling the timing of expression of the different classes of genes involved in infection. In addition, the interplay between environmental signals and positive feedback aids the bacterium identify precise conditions for and control expression of virulence genes.
Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Bordetella/fisiologia , Retroalimentação Fisiológica , Regulação Bacteriana da Expressão Gênica , Proteínas de Bactérias/química , Bordetella/patogenicidade , Modelos Biológicos , Fosforilação , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas , Transdução de Sinais , Virulência , Fatores de Virulência/genéticaRESUMO
No disponible
Assuntos
Humanos , Infecções por Bordetella/complicações , Infecções Respiratórias/microbiologia , Bordetella/patogenicidadeAssuntos
Infecções por Bordetella/microbiologia , Bordetella/isolamento & purificação , Infecções Respiratórias/microbiologia , Idoso de 80 Anos ou mais , Bordetella/classificação , Bordetella/patogenicidade , Infecções por Bordetella/complicações , Infecções por Bordetella/diagnóstico , Coinfecção , Farmacorresistência Bacteriana Múltipla , Feminino , Humanos , Imunocompetência , Klebsiella/isolamento & purificação , Infecções por Klebsiella/complicações , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/microbiologia , Testes de Sensibilidade Microbiana , Infecções Respiratórias/diagnóstico , Ribotipagem , Escarro/microbiologiaAssuntos
Bordetella/isolamento & purificação , Pé Diabético/microbiologia , Achromobacter/isolamento & purificação , Idoso , Antibacterianos/uso terapêutico , Bordetella/patogenicidade , Ceftazidima/uso terapêutico , Coinfecção/tratamento farmacológico , Infecções por Corynebacterium/tratamento farmacológico , Infecções por Corynebacterium/microbiologia , Pé Diabético/complicações , Feminino , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico , Vancomicina/uso terapêutico , Infecção dos Ferimentos/microbiologiaRESUMO
Sensing the environment allows pathogenic bacteria to coordinately regulate gene expression to maximize survival within or outside of a host. Here we show that Bordetella species regulate virulence factor expression in response to carbon dioxide levels that mimic in vivo conditions within the respiratory tract. We found strains of Bordetella bronchiseptica that did not produce adenylate cyclase toxin (ACT) when grown in liquid or solid media with ambient air aeration, but produced ACT and additional antigens when grown in air supplemented to 5% CO(2). Transcriptome analysis and quantitative real time-PCR analysis revealed that strain 761, as well as strain RB50, increased transcription of genes encoding ACT, filamentous hemagglutinin (FHA), pertactin, fimbriae and the type III secretion system in 5% CO(2) conditions, relative to ambient air. Furthermore, transcription of cyaA and fhaB in response to 5% CO(2) was increased even in the absence of BvgS. In vitro analysis also revealed increases in cytotoxicity and adherence when strains were grown in 5% CO(2). The human pathogens B. pertussis and B. parapertussis also increased transcription of several virulence factors when grown in 5% CO(2), indicating that this response is conserved among the classical bordetellae. Together, our data indicate that Bordetella species can sense and respond to physiologically relevant changes in CO(2) concentrations by regulating virulence factors important for colonization, persistence and evasion of the host immune response.
Assuntos
Infecções por Bordetella/microbiologia , Bordetella/genética , Bordetella/metabolismo , Dióxido de Carbono/metabolismo , Regulação Bacteriana da Expressão Gênica , Fatores de Virulência de Bordetella/metabolismo , Toxina Adenilato Ciclase/genética , Toxina Adenilato Ciclase/metabolismo , Animais , Bordetella/patogenicidade , Bordetella bronchiseptica/genética , Bordetella bronchiseptica/metabolismo , Bordetella bronchiseptica/patogenicidade , Bordetella parapertussis/genética , Bordetella parapertussis/metabolismo , Bordetella parapertussis/patogenicidade , Bordetella pertussis/genética , Bordetella pertussis/metabolismo , Bordetella pertussis/patogenicidade , Linhagem Celular , Perfilação da Expressão Gênica , Humanos , Macrófagos/microbiologia , Camundongos , Camundongos Endogâmicos C57BL , Fatores de Virulência de Bordetella/genéticaRESUMO
BACKGROUND: The classical Bordetella subspecies are phylogenetically closely related, yet differ in some of the most interesting and important characteristics of pathogens, such as host range, virulence and persistence. The compelling picture from previous comparisons of the three sequenced genomes was of genome degradation, with substantial loss of genome content (up to 24%) associated with adaptation to humans. RESULTS: For a more comprehensive picture of lineage evolution, we employed comparative genomic and phylogenomic analyses using seven additional diverse, newly sequenced Bordetella isolates. Genome-wide single nucleotide polymorphism (SNP) analysis supports a reevaluation of the phylogenetic relationships between the classical Bordetella subspecies, and suggests a closer link between ovine and human B. parapertussis lineages than has been previously proposed. Comparative analyses of genome content revealed that only 50% of the pan-genome is conserved in all strains, reflecting substantial diversity of genome content in these closely related pathogens that may relate to their different host ranges, virulence and persistence characteristics. Strikingly, these analyses suggest possible horizontal gene transfer (HGT) events in multiple loci encoding virulence factors, including O-antigen and pertussis toxin (Ptx). Segments of the pertussis toxin locus (ptx) and its secretion system locus (ptl) appear to have been acquired by the classical Bordetella subspecies and are divergent in different lineages, suggesting functional divergence in the classical Bordetellae. CONCLUSIONS: Together, these observations, especially in key virulence factors, reveal that multiple mechanisms, such as point mutations, gain or loss of genes, as well as HGTs, contribute to the substantial phenotypic diversity of these versatile subspecies in various hosts.
Assuntos
Bordetella/genética , Bordetella/patogenicidade , Transferência Genética Horizontal , Genoma Bacteriano , Antígenos O/genética , Toxina Pertussis/genética , Fatores de Virulência de Bordetella/genética , Animais , Evolução Biológica , Bordetella/classificação , Mapeamento Cromossômico , Variação Genética , Estudo de Associação Genômica Ampla , Genômica , Especificidade de Hospedeiro , Humanos , Filogenia , Polimorfismo de Nucleotídeo Único , Ovinos , Especificidade da Espécie , VirulênciaRESUMO
Over the past few decades, our understanding of the bacterial protein toxins that modulate G proteins has advanced tremendously through extensive biochemical and structural analyses. This article provides an updated survey of the various toxins that target G proteins, ending with a focus on recent mechanistic insights in our understanding of the deamidating toxin family. The dermonecrotic toxin from Pasteurella multocida (PMT) was recently added to the list of toxins that disrupt G-protein signal transduction through selective deamidation of their targets. The C3 deamidase domain of PMT has no sequence similarity to the deamidase domains of the dermonecrotic toxins from Escherichia coli (cytotoxic necrotizing factor [CNF]1-3), Yersinia (CNFY) and Bordetella (dermonecrotic toxin). The structure of PMT-C3 belongs to a family of transglutaminase-like proteins, with active site Cys-His-Asp catalytic triads distinct from E. coli CNF1.
Assuntos
Toxinas Bacterianas/metabolismo , Citotoxinas/metabolismo , Proteínas de Ligação ao GTP/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Bordetella/patogenicidade , Desaminação , Escherichia coli/patogenicidade , Modelos Biológicos , Pasteurella multocida/patogenicidade , Estrutura Terciária de Proteína , Homologia de Sequência de Aminoácidos , Yersinia/patogenicidadeRESUMO
Pertactin (PRN) is an autotransporter protein produced by all members of the Bordetella bronchiseptica cluster, which includes B. pertussis, B. parapertussis, and B. bronchiseptica. It is a primary component of acellular pertussis vaccines, and anti-PRN antibody titers correlate with protection. In vitro studies have suggested that PRN functions as an adhesin and that an RGD motif located in the center of the passenger domain is important for this function. Two regions of PRN that contain sequence repeats (region 1 [R1] and R2) show polymorphisms among strains and have been implicated in vaccine-driven evolution. We investigated the role of PRN in pathogenesis using B. bronchiseptica and natural-host animal models. A Deltaprn mutant did not differ from wild-type B. bronchiseptica in its ability to adhere to epithelial and macrophage-like cells in vitro or to establish respiratory infection in rats but was cleared much faster than wild-type bacteria in a mouse lung inflammation model. Unlike wild-type B. bronchiseptica, the Deltaprn mutant was unable to cause a lethal infection in SCID-Bg mice, but, like wild-type bacteria, it was lethal for neutropenic mice. These results suggest that PRN plays a critical role in allowing Bordetella to resist neutrophil-mediated clearance. Mutants producing PRN proteins in which the RGD motif was replaced with RGE or in which R1 and R2 were deleted were indistinguishable from wild-type bacteria in all assays, suggesting that these sequences do not contribute to PRN function.
Assuntos
Proteínas da Membrana Bacteriana Externa/fisiologia , Infecções por Bordetella/microbiologia , Bordetella/patogenicidade , Neutrófilos/imunologia , Animais , Aderência Bacteriana/genética , Aderência Bacteriana/fisiologia , Proteínas da Membrana Bacteriana Externa/genética , Bordetella/genética , Infecções por Bordetella/imunologia , Bordetella bronchiseptica/genética , Bordetella bronchiseptica/patogenicidade , Epitélio/microbiologia , Feminino , Pulmão/imunologia , Pulmão/microbiologia , Macrófagos Alveolares/microbiologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos SCID , Neutropenia/imunologia , Neutropenia/microbiologia , Neutrófilos/fisiologia , Engenharia de Proteínas , Ratos , Ratos Wistar , Fatores de Virulência de Bordetella/genéticaRESUMO
Bordetella pertussis, the etiological agent of whooping cough, belongs to the bacterial pathogens first described in the so-called golden era of microbiology more than 100 years ago. In the course of the following decades, several other closely related pathogens were described which are nowadays classified in the genus Bordetella together with B. pertussis. These are the human and animal pathogens B. parapertussis, B. bronchiseptica and B. avium which are of high medical or veterinary interest, and which, together with B. pertussis, are referred to as the "classical" Bordetella species. Only in the past 15 years, several additional species were classified in the genus, frequently isolated from patients with underlying disease, animals or from the environment. Very little is known about most of these bacteria. In the present review, the current knowledge about these "new" Bordetella species is briefly summarized.