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1.
PLoS Pathog ; 15(9): e1008085, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31568486

RESUMO

Human parasitic nematodes are the causative agents of lymphatic filariasis (elephantiasis) and onchocerciasis (river blindness), diseases that are endemic to more than 80 countries and that consistently rank in the top ten for the highest number of years lived with disability. These filarial nematodes have evolved an obligate mutualistic association with an intracellular bacterium, Wolbachia, a symbiont that is essential for the successful development, reproduction, and survival of adult filarial worms. Elimination of the bacteria causes adult worms to die, making Wolbachia a primary target for developing new interventional tools to combat filariases. To further explore Wolbachia as a promising indirect macrofilaricidal drug target, the essential cellular processes that define the symbiotic Wolbachia-host interactions need to be identified. Genomic analyses revealed that while filarial nematodes encode all the enzymes necessary for glycolysis, Wolbachia does not encode the genes for three glycolytic enzymes: hexokinase, 6-phosphofructokinase, and pyruvate kinase. These enzymes are necessary for converting glucose into pyruvate. Wolbachia, however, has the full complement of genes required for gluconeogenesis starting with pyruvate, and for energy metabolism via the tricarboxylic acid cycle. Therefore, we hypothesized that Wolbachia might depend on host glycolysis to maintain a mutualistic association with their parasitic host. We did conditional experiments in vitro that confirmed that glycolysis and its end-product, pyruvate, sustain this symbiotic relationship. Analysis of alternative sources of pyruvate within the worm indicated that the filarial lactate dehydrogenase could also regulate the local intracellular concentration of pyruvate in proximity to Wolbachia and thus help control bacterial growth via molecular interactions with the bacteria. Lastly, we have shown that the parasite's pyruvate kinase, the enzyme that performs the last step in glycolysis, could be a potential novel anti-filarial drug target. Establishing that glycolysis is an essential component of symbiosis in filarial worms could have a broader impact on research focused on other intracellular bacteria-host interactions where the role of glycolysis in supporting intracellular survival of bacteria has been reported.


Assuntos
Brugia/metabolismo , Brugia/microbiologia , Ácido Pirúvico/metabolismo , Wolbachia/metabolismo , Animais , Brugia/genética , Brugia Malayi/genética , Brugia Malayi/metabolismo , Brugia Malayi/microbiologia , Brugia pahangi/genética , Brugia pahangi/metabolismo , Brugia pahangi/microbiologia , Feminino , Filariose/metabolismo , Filariose/microbiologia , Filariose/parasitologia , Genes de Helmintos , Glicólise , Interações entre Hospedeiro e Microrganismos , Interações Hospedeiro-Parasita , Humanos , Masculino , Simbiose , Wolbachia/genética
2.
Expert Rev Anti Infect Ther ; 7(5): 595-605, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19485799

RESUMO

Lymphatic filariasis is a major cause of acute and chronic morbidity in 81 countries. The availability of safe treatment regimens along with rapid diagnostic tools resulted in a global program to eliminate the disease. The two main objectives of the global elimination program are to interrupt transmission of the parasites and to provide care for those with the disease. The strategy for transmission interruption is preventive chemotherapy through mass drug administration. This article reviews the current treatment regimens for lymphatic filariasis and discusses the challenges posed by co-endemicity with other diseases. The role of integrated vector management as a supplementary strategy for mass drug administration and new strategies for treatment and morbidity control through antibiotic targeting of the Wolbachia endosymbionts are also discussed.


Assuntos
Filariose Linfática/tratamento farmacológico , Filariose Linfática/prevenção & controle , Filaricidas/uso terapêutico , Animais , Brugia/efeitos dos fármacos , Brugia/microbiologia , Culex/parasitologia , Atenção à Saúde , Filariose Linfática/parasitologia , Filariose Linfática/transmissão , Humanos , Insetos Vetores/parasitologia , Microfilárias/efeitos dos fármacos , Wolbachia/efeitos dos fármacos , Wuchereria bancrofti/efeitos dos fármacos , Wuchereria bancrofti/microbiologia
3.
Cell Microbiol ; 6(2): 97-104, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-14706096

RESUMO

In this review, we describe the pathogenic role of Wolbachia endosymbiotic bacteria in filarial diseases, focusing on the host innate immune responses to filarial and Wolbachia products. A description of the host pathogen recognition and early inflammatory responses including TLR4-mediated signalling, chemokine and cytokine responses and inflammatory cell recruitment is provided from human studies and from animal models of filarial disease. Finally, the impact of the discovery and characterization of Wolbachia on filarial research and treatment programmes is discussed.


Assuntos
Brugia/microbiologia , Filariose/imunologia , Filariose/fisiopatologia , Onchocerca/microbiologia , Simbiose , Wolbachia/imunologia , Animais , Brugia/imunologia , Brugia/patogenicidade , Filariose/parasitologia , Humanos , Onchocerca/imunologia , Onchocerca/patogenicidade , Wolbachia/crescimento & desenvolvimento
4.
Curr Opin Investig Drugs ; 3(4): 533-7, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12090719

RESUMO

More effective drugs are needed for the treatment of human filarial diseases and the elimination of these infections as a public health problem. The drugs must either kill or sterilize adult worms. The relevant filariae, Onchocerca volvulus, Wuchereria bancofti and Brugia species, harbor rickettsial endoboacteria of the genus Wolbachia as symbionts. Animal experiments have shown that the elimination of these endobacteria causes inhibition of embryogenesis, and with Onchocerca ochengi a macrofilaricidal effect. Trials with human onchocerciasis patients using doxycydine demonstrated a long-term sterilizing activity, opening up a new strategy for the control of filarial infections. Indications of antiwolbachial therapy against onchocerciasis are discussed.


Assuntos
Filariose/tratamento farmacológico , Filaricidas/uso terapêutico , Animais , Brugia/microbiologia , Doxiciclina/administração & dosagem , Doxiciclina/uso terapêutico , Resistência a Medicamentos , Filariose/parasitologia , Humanos , Onchocerca/microbiologia , Oncocercose/tratamento farmacológico , Oncocercose/parasitologia , Simbiose , Wolbachia/efeitos dos fármacos , Wuchereria bancrofti/microbiologia
5.
Ann Trop Med Parasitol ; 96(8): 809-21, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12625936

RESUMO

Brugia timori is widely distributed on Alor Island, Indonesia, where it causes a high degree of morbidity. The HhaI tandem repeat of B. timori was found to be identical to that of B. malayi, for which sensitive PCR-based assays have already been developed. Using one of these assays, a single microfilaria (mf) of B. timori, present in a spot of dry blood on filter paper, could be detected. The assay was equally sensitive in the detection of B. timori and B. malayi. When the collected mosquitoes were pooled according to species and tested with the assay, 39 (64%) of the 61 Anopheles barbirostris pools (containing a total of 642 mosquitoes) were positive. As none of the 33 Culex pools tested (which contained 624 mosquitoes) gave a positive result, and An. barbirostris is the only Anopheles species commonly caught on human bait in Alor, An. barbirostris is assumed to be the main and perhaps only local vector. Brugia timori could be differentiated from B. malayi by restriction-endonuclease digestion of the PCR-amplified mitochondrial cytochrome oxidase subunit 2. A few distinct nucleotide exchanges were also found in the second internal transcribed ribosomal spacer of the filariae, and in the 16S rDNA and FTSZ gene of their Wolbachia endobacteria. The results show that B. timori can be effectively detected using the PCR-based assay developed for B. malayi and can then be differentiated from B. malayi by other molecular markers. PCR-based techniques targeting the HhaI repeat can therefore be employed for monitoring B. timori in the framework of the Global Programme to Eliminate Lymphatic Filariasis.


Assuntos
Brugia/genética , DNA de Helmintos/análise , Filariose Linfática/parasitologia , Reação em Cadeia da Polimerase/métodos , Animais , Anopheles/parasitologia , Brugia/isolamento & purificação , Brugia/microbiologia , Brugia Malayi/genética , Brugia Malayi/isolamento & purificação , Brugia pahangi/genética , Brugia pahangi/isolamento & purificação , DNA Espaçador Ribossômico/análise , Humanos , Indonésia , Dados de Sequência Molecular , Sequências Repetitivas de Ácido Nucleico , Homologia de Sequência do Ácido Nucleico , Wolbachia/genética
7.
Int J Parasitol ; 29(2): 357-64, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10221636

RESUMO

Wolbachia endosymbiotic bacteria have been shown to be widespread among filarial worms and could thus play some role in the biology of these nematodes. Indeed, tetracycline has been shown to inhibit both the development of adult worms from third-stage larvae and the development of the microfilaraemia in jirds infected with Brugia pahangi. The possibility that these effects are related to the bacteriostatic activity of tetracycline on Wolbachia symbionts should be considered. Here we show that tetracycline treatment is very effective in blocking embryo development in two filarial nematodes, B. pahangi and Dirofilaria immitis. Embryo degeneration was documented by TEM, while the inhibition of the transovarial transmission of Wolbachia was documented by PCR. Phylogenetic analysis on the ssrDNA sequence of the Wolbachia of B. pahangi confirms that the phylogeny of the bacterial endosymbionts is consistent with that of the host worms. The possibility that tetracycline inhibition of embryo development in B. pahangi and D. immitis is determined by cytoplasmic incompatibility is discussed.


Assuntos
Antibacterianos/farmacologia , Brugia/efeitos dos fármacos , Dirofilaria/efeitos dos fármacos , Rickettsiaceae/efeitos dos fármacos , Tetraciclina/farmacologia , Animais , Brugia/microbiologia , Dirofilaria/microbiologia , Cães , Filariose Linfática/veterinária , Feminino , Gerbillinae/parasitologia , Microscopia Eletrônica , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/métodos , Rickettsiaceae/genética , Rickettsiaceae/isolamento & purificação , Simbiose
8.
J Parasitol ; 63(6): 992-1000, 1977 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-592054

RESUMO

Observation of intracellular organisms in the lateral chords of Brugia malayi adults initiated further studies to determine the prevalence of these organisms within the tissues of adult worms and of larvae. The organisms were found in the lateral chords of adult males and females, microfilariae, first-, second-, third-, and fourth-stage larvae. In the females, they were present in the oogonia, oocytes, and developing eggs, suggesting transovarial transmission within the life cycle of the filarid. The organisms may have a developmental cycle consisting of more than one stage, including a small spheroidal stage up to 0.6 micrometer in size and a larger form up to 1.5 micrometer in length, all of which occur in the cytoplasm within a vesicle formed of host membrane. Each stage lacks a definite cell wall, being bound by 2 trilaminate membranes. The bacterial entities in B. malayi resemble both in morphology and development the organisms found in other filarids, but whether they affect the vertebrate host in any way remains to be determined. Their presence within certain cells of the developing eggs could be exploited as intracellular markers for the organogenesis of the lateral chords and the ovary.


Assuntos
Brugia/microbiologia , Filarioidea/parasitologia , Aedes/parasitologia , Animais , Brugia/ultraestrutura , Gatos/parasitologia , Feminino , Filarioidea/microbiologia , Ovário/microbiologia , Ovário/ultraestrutura
9.
Trans R Soc Trop Med Hyg ; 69(5-6): 509-14, 1975.
Artigo em Inglês | MEDLINE | ID: mdl-1228988

RESUMO

Unusual bodies have been described in the hypodermal tissues of larval Dirofilaria immitis and Brugia pahangi. Ultrastructural evidence indicates that these bodies are probably Gram-negative micro-organisms. It appears that the presence of large numbers of these bodies in an early embryo may affect development adversely. Their importance at later stages of development of filariae is not known.


Assuntos
Brugia/microbiologia , Dirofilaria immitis/microbiologia , Filarioidea/microbiologia , Animais , Brugia/ultraestrutura , Dirofilaria immitis/ultraestrutura , Feminino , Larva , Microscopia de Fluorescência
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