RESUMO
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
Assuntos
Antígenos de Helmintos/análise , Brugia pahangi/química , Proteínas de Helminto/análise , Sequência de Aminoácidos , Animais , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/química , Antígenos de Helmintos/imunologia , Brugia pahangi/imunologia , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Filariose Linfática/sangue , Filariose Linfática/imunologia , Filariose Linfática/parasitologia , Gerbillinae , Proteínas de Helminto/química , Proteínas de Helminto/imunologia , Humanos , Processamento de Imagem Assistida por Computador , Immunoblotting , Dados de Sequência Molecular , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
A fragment of a cut-1 like gene from the filarial nematode Brugia pahangi (designated Bp-cut-1) was isolated by PCR from genomic DNA. The sequence was used to design primers for use in RT-PCR and resulted in the isolation of a cDNA fragment from larvae in the process of the L3-L4 moult. Screening of a B. malayi genomic library identified a single clone, Bm-cut-1. Using primers designed from the Brugia sequences, semi-quantitative RT-PCR was carried out on 11 different life cycle stages chosen to cover periods around the moult and inter-moult periods. This analysis demonstrated that the cut-1 mRNA was most abundant preceding the moult, consistent with its function as a cuticular protein. Immuno-gold electron microscopy using an affinity purified antiserum raised to the highly conserved region of Ascaris CUT-1 confirmed that the protein was restricted to a tight band in the median layer of the cuticle. Despite the fact that no transcripts could be detected in mature adult worms by RT-PCR, immuno-gold microscopy revealed staining of the microfilarial cuticle within the uterus of the adult female worm, suggesting that other cut-1-like genes are present in Brugia.
Assuntos
Brugia Malayi/genética , Brugia pahangi/genética , Proteínas de Caenorhabditis elegans , Genes de Helmintos , Proteínas de Helminto/genética , Sequência de Aminoácidos , Animais , Brugia Malayi/química , Brugia Malayi/crescimento & desenvolvimento , Brugia pahangi/química , Brugia pahangi/crescimento & desenvolvimento , Clonagem Molecular , DNA Complementar/genética , Feminino , Biblioteca Gênica , Proteínas de Helminto/análise , Proteínas de Helminto/química , Imuno-Histoquímica , Masculino , Microscopia Eletrônica , Dados de Sequência Molecular , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Transforming growth factor-beta (TGF-beta) superfamily genes encode products controlling pattern formation, cell differentiation, and immune-mediated inflammation. Members of this superfamily are known in multicellular organisms from mammals to the model nematode Caenorhabditis elegans. Using PCR with oligonucleotides complementary to highly conserved motifs in the TGF-beta superfamily, we first isolated a genomic clone from the filarial nematode Brugia malayi. This gene, termed Bm-tgh-1 (TGF-beta homolog-1), spans 2.5 kb of genomic DNA and contains seven exons. Transcripts of this gene are poorly represented in cDNA libraries, but a full-length cDNA was isolated by RACE from B. pahangi (Bp-tgh-1). The tgh-1 genes from the two species are >98% identical at the nucleotide and amino acid levels, differing at 18/1576 base pairs and 5/428 amino acids; all nonsynonymous substitutions are in the long N-terminal propeptide. They show a high level of similarity throughout all seven exons to a C. elegans gene on cosmid T25F10. Homology to other members of the TGF-beta superfamily is restricted to the C-terminal domain which contains the mature active protein. Key features shared with other members of the superfamily include the tetrabasic proteolytic cleavage site to release an active C-terminal peptide, seven cysteines arrayed in identical fashion, and conserved sequence motifs. tgh-1 is most similar to the BMP-1 subfamily involved in developmental signaling in nematodes, insects, and vertebrates. RT-PCR on first-strand cDNA from both Brugia species, with primers specific to the 3' end, showed that tgh-1 is not expressed in the microfilarial stage, but is detectable in the mosquito-derived infective larvae and is maximal in maturing parasites around the time of molting in the mammalian host. Adult parasites show a relatively low level of expression. The identification of tgh-1, and its preferential expression in developing parasites, suggests that it may be involved in key developmental events in the complex filarial life cycle.
Assuntos
Brugia Malayi/química , Brugia pahangi/química , Fator de Crescimento Transformador beta/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Brugia Malayi/genética , Brugia pahangi/genética , DNA de Helmintos/química , DNA de Helmintos/isolamento & purificação , Éxons , Feminino , Expressão Gênica , Masculino , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , RNA Mensageiro/análise , Alinhamento de Sequência , Fator de Crescimento Transformador beta/químicaRESUMO
The mechanism of linkage of phosphorylcholine (PC) to excretory-secretory products (ES) of adult Brugia pahangi has been investigated. Biosynthetic radio-isotope labelling of ES with [3H]choline followed by SDS-PAGE/fluorography revealed a smear of molecular weight approximately 40-100 kDa which loses its radiolabel following exposure to N-glycosidase F, but not mild alkali. PC is thus attached to this smear of molecules via N-type glycans, a mechanism of linkage previously observed with respect to PC-ES of Acanthocheilonema viteae. Western blotting analysis of non-radiolabelled ES demonstrated the existence of additional PC-ES which were insensitive to N-glycosidase F, but not to alkali. This second group of molecules is therefore likely to contain PC linked to O-glycans. Filarial nematodes may thus utilize 2 classes of glycan for attachment of PC. Examination of B. pahangi and A. viteae whole worm extracts by Western blotting indicated that their PC content could not be cleaved by N-glycosidase F and hence the use of N-type glycans may be restricted to a subset of ES products. The implications of these findings with respect to developing inhibitors of PC attachment for use as anti-filarial drugs are discussed.
Assuntos
Antígenos de Helmintos/metabolismo , Brugia pahangi/química , Proteínas de Helminto/metabolismo , Fosforilcolina/metabolismo , Polissacarídeos/metabolismo , Amidoidrolases/metabolismo , Animais , Antígenos de Helmintos/química , Western Blotting , Brugia pahangi/imunologia , Eletroforese em Gel de Poliacrilamida , Proteínas de Helminto/química , Peso Molecular , Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase , Fosforilcolina/química , Polissacarídeos/químicaRESUMO
The cuticle of filarial nematodes is a specialized extracellular matrix that covers the parasite and protects it from adverse conditions of the environment. As a surface structure it is in direct contact with the host defence mechanisms and therefore plays an important role in the molecular host-parasite relationship. Using polyclonal antisera raised against the insoluble components of the cuticle of the adult filarial parasite Brugia pahangi, we have isolated cDNA clones encoding collagen molecules of the cuticle. The protein domain structure of cDNA clone Bpcol-1 was compared with the known structures of cuticular collagens of the nematodes Brugia malayi, Caenorhabditis elegans, Ascaris suum and Haemonchus contortus, confirming interspecies similarities. Using affinity-purified anti-Bpcol-1 antibodies we identified Bpcol-1 antigenic determinants in different nematode extracts, and determined the localization of such epitopes within the cuticle of B. pahangi.
Assuntos
Antígenos de Helmintos , Brugia pahangi/química , Colágeno/genética , Proteínas de Helminto/genética , Sequência de Aminoácidos , Animais , Brugia pahangi/genética , Clonagem Molecular , Colágeno/análise , Colágeno/química , Reações Cruzadas , DNA Complementar/genética , DNA de Helmintos/genética , Epitopos/análise , Proteínas de Helminto/análise , Proteínas de Helminto/química , Dados de Sequência Molecular , Peso Molecular , Especificidade de Órgãos , Estrutura Secundária de Proteína , RNA de Helmintos/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da EspécieAssuntos
Drosophila melanogaster/química , Proteínas Ribossômicas/análise , Sequência de Aminoácidos , Animais , Brugia pahangi/química , Halobacterium/química , Dados de Sequência Molecular , Ratos , Proteínas Ribossômicas/química , Proteínas Ribossômicas/genética , Homologia de Sequência de AminoácidosRESUMO
The cuticle of filarial nematode parasites contains distinct and separable sets of soluble and structural proteins. Surface-labeling techniques have previously identified a soluble protein complex in adult stage Brugia which ranges in molecular weight from 15 to 200 kDa. Using an antiserum directed to the 15-kDa basal subunit of this complex, we show here that this complex is synthesized and processed from a single, very large precursor protein with a molecular weight of approximately 400 kDa. Molecular cloning, sequencing, and Southern analysis indicates that the protein is encoded by a single gene composed predominantly of approximately 20 tandemly repeated segments of 396 bp. The two complete copies of these repeated segments in our cDNA sequence are identical. Each subunit of 132 amino acids bears a consensus site for N-linked glycosylation, and glycosidase treatment indicates that this corresponds to an oligosaccharide side chain of 2 kDa. The protein displays no significant homology to sequences lodged in databases corresponding to molecules of known function. Nevertheless, a significant similarity (19/41 residues) is observed with the N-terminal sequence of a protein termed ABA-1, an allergen from Ascaris.