Study of the lens epithelial cell density in cataractous eyes operated on with extracapsular and intercapsular techniques.

Epithelial cell counting was performed in 47 fragments from lens anterior capsules obtained during extracapsular and intercapsular surgery. Mean cell count was 3,277 cell/mm2; there was no correlation between cell number and age and no significant difference in cell counts between extracapsular and intercapsular surgery. There were, however, significant differences between advanced cataracts and the other cataract types (2,947 vs. 3,356). This may be important in explaining the differences in regenerative opacification of the posterior capsule in the different types of cataracts.

Synthesis of lens capsule and plasma membrane glycoproteins by lens epithelial cells and fibers in the rat.

The lens of the eye possesses a capsule which is a greatly hypertrophied basement membrane. To investigate the synthesis of glycoproteins destined for this capsule, 3H-fucose was injected into the vitreous body of intact rats weighing approximately 200 gm. The animals were killed from 10 min to 14.5 months later, and their lenses were processed for electron microscope radioautography. At 10 min after injection, more than 58% of the silver grains were localized to the Golgi apparatus of the lens epithelial cells. By day 1, the heaviest sites of reaction were the plasma membrane (more than 50% of total label), the basal cytoplasm, and the adjacent lens capsule, where a heavy band of reaction was seen. The remainder of the capsule exhibited a lighter diffuse reaction. In the lens fibers, the label was at first localized to clusters of vesicles but then migrated to the plasma membrane and to the region of the capsule adjacent to the basal surface of these fibers. Light microscope radioautographs of the lens capsule at later time intervals revealed that by 1 month after injection the diffuse reaction had disappeared, and only the strongly labeled band remained. By 14.5 months after injection, this band had migrated partially across the lens capsule, but the capsule itself had increased considerably in thickness. On the other hand, the distance between the labeled band and the free edge of the capsule had decreased from that seen at the time of injection.