RESUMO
Although phosphatidylserine synthase (PSS) from Escherichia coli is an ideal enzyme for phospholipid production, its application in the food industry has been limited because of the low PSS yield. In this study, the pss gene was cloned from E. coli K(12) and expressed in Bacillus subtilis DB104, and the recombinant PSS was characterized subsequently. PSS was purified to 39.59-fold, and the highest activity was detected as 13.62 U/mg. The enzyme was found to be stable in a pH range of 6.5-9.5, with optimal pH values of 8.0 for hydrolysis and 7.0 for transphosphatidylation, respectively. The optimal temperature for PSS activity was 35 degrees C. The enzyme activity could be detected after 1 h of heating at 65 degrees C. Among the detected detergents and metal ions, Triton X-100, Ca(2+), Mn(2+), and Co(2+) could improve PSS activity. The transformation of phosphatidylcholine to phosphatidylserine under PSS catalyzation was carried out in a biphasic system, which confirmed the actual catalyzing ability of the recombinant protein.
Assuntos
Bacillus subtilis/genética , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/genética , Escherichia coli K12/enzimologia , Expressão Gênica , Sequência de Aminoácidos , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Clonagem Molecular , Estabilidade Enzimática , Temperatura Alta , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Transformação BacterianaRESUMO
The activity of phosphatidylserine (PS) synthase (CDP-1, 2-diacyl-sn-glycerol: l-serine O-phosphatidyltransferase, EC 2.7.8. 8) from Escherichia coli was studied after reconstitution with lipid vesicles of various compositions. PS synthase exhibited practically no activity in the absence of a detergent and with the substrate CDP-diacylglycerol (CDP-DAG) present only in the lipid vesicles. Inclusion of octylglucoside (OG) in the assay mixture increased the activity 20- to 1000-fold, the degree of activation depending on the lipid composition of the vesicles. Inclusion of additional CDP-DAG in the assay mixture increased the activity 5- to 25-fold. When the fraction of phosphatidylglycerol (PG) was increased from 15 to 100 mol% in the vesicles the activity increased 10-fold using the assay mixture containing OG. The highest activities were exhibited with the anionic lipids synthesized by E. coli, namely PG, diphosphatidylglycerol (DPG), and phosphatidic acid, while phosphatidylinositol gave a lower activity. Cryotransmission electron microscopy showed that transformation of the vesicles to micelles brings about an activation of the enzyme that is proportional to the degree of micellization. Thus, the activity of PS synthase is modulated by the lipid aggregate structure and by the fraction and type of anionic phospholipid in the aggregates. The increase in the activity caused by PG and DPG is physiologically relevant; it may be part of a regulatory mechanism that keeps the balance between phosphatidylethanolamine, and the sum of PG and DPG, nearly constant in wild-type E. coli cells.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Escherichia coli/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Sistema Livre de Células , Detergentes , Ativação Enzimática , Glucosídeos , Lipídeos de Membrana/química , Lipídeos de Membrana/farmacologia , Micelas , Conformação Molecular , Fosfolipídeos/química , Fosfolipídeos/farmacologia , Proteolipídeos/química , Proteolipídeos/ultraestrutura , SolubilidadeRESUMO
In the yeast, Saccharomyces cerevisiae, similar to higher eukaryotes most phospholipids are synthesized in microsomes. Mitochondria contribute to the cellular biosynthesis of phospholipids insofar as they harbor phosphatidylethanolamine decarboxylase, and enzymes of phosphatidylglycerol and cardiolipin synthesis. In this paper we present evidence that certain enzymes of phospholipid biosynthesis, namely phosphatidylserine and phosphatidylinositol synthase, are enriched in a special microsomal fraction associated with mitochondria, which we named MAM. This fraction was isolated and characterized with respect to marker enzymes, protein and phospholipid composition, and enzymes of phospholipid synthesis. According to these analyses MAMs are a specialized subfraction of the endoplasmic reticulum, which is distinct from other microsomal subfractions. Phosphatidylserine synthesized in MAMs can be readily imported into mitochondria and converted to phosphatidylethanolamine. Reassociation of MAMs with purified mitochondria led to reconstitution of the import of phosphatidylserine into mitochondria. Organelle contact is suggested as a possible mechanism of this process.
Assuntos
Microssomos/metabolismo , Mitocôndrias/metabolismo , Fosfolipídeos/metabolismo , Saccharomyces cerevisiae/metabolismo , CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferase , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Carboxiliases/metabolismo , Fracionamento Celular , Centrifugação com Gradiente de Concentração , Eletroforese em Gel de Poliacrilamida , Microssomos/ultraestrutura , Mitocôndrias/ultraestrutura , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Fosfolipídeos/biossíntese , Saccharomyces cerevisiae/ultraestrutura , Esferoplastos/metabolismo , Esferoplastos/ultraestrutura , Transferases (Outros Grupos de Fosfato Substituídos)/isolamento & purificação , Transferases (Outros Grupos de Fosfato Substituídos)/metabolismoRESUMO
To better establish the intracellular location of the phosphatidylserine synthase of Escherichia coli and hence better understand how it is regulated in the cell, we compared the size, function, and binding properties of the enzyme made in vitro with the enzyme found in cell lysates and with the purified enzyme. The enzyme made either in vivo or in an active form in vitro was found primarily associated with the ribosomal fraction of the cell and had the same apparent molecular mass as the purified enzyme. These results were unaffected by the presence of protease inhibitors. Addition of unsupplemented E. coli membranes or membranes supplemented with phosphatidylethanolamine did not affect the subcellular distribution of the enzyme in these experiments. However, addition of membranes supplemented with either the lipid substrate, CDP-diacylglycerol, or the lipid product, phosphatidylserine, resulted in membrane association by the enzyme rather than ribosomal association. Addition of membranes supplemented with acidic lipids also brought about membrane association, but this association was primarily ionic since it was disrupted by high salt concentrations. These results strongly suggest that the ribosomal location of this enzyme is not the result of some modification event occurring after cell lysis and that the normal functioning of the enzyme involves membrane association which is primarily induced by the presence of a membrane-associated substrate.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Escherichia coli/enzimologia , Fosfotransferases/metabolismo , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/biossíntese , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Membrana Celular/enzimologia , Diglicerídeos de Citidina Difosfato/análise , Diglicerídeos de Citidina Difosfato/metabolismo , Peso Molecular , Fosfatidilserinas/análise , Fosfatidilserinas/metabolismo , Ribossomos/enzimologiaRESUMO
A CDP-diacylglycerol-dependent phosphatidylserine synthase was solubilized from Bacillus licheniformis membranes and purified to near homogeneity. The purification procedure consisted of CDP-diacylglycerol-Sepharose affinity chromatography followed by substrate elution from blue dextran-Sepharose. The purified preparation showed a single band with an apparent relative molecular mass of 53 000 daltons when subjected to sodium dodecyl sulfate--polyacrylamide gel electrophoresis. Proteolytic digestion of the enzyme yielded a smaller (41 000 daltons) active form. The preparation was free of any phosphatidylglycerophosphate synthase, phosphatidylserine decarboxylase, CDP-diacylglycerol hydrolase, and phosphatidylserine hydrolase activities. The utilization of substrates and the formation of products occurred with the expected stoichiometry. Radioisotopic exchange patterns between related substrate and product pairs suggest a sequential Bi-Bi reaction as opposed to the ping-pong mechanism exhibited by the well-studied phosphatidylserine synthase of Escherichia coli [Larson, T. J., & Dowhan, W. (1976) Biochemistry 15, 5212-5218]. The B. licheniformis enzyme was also found to be markedly dissimilar to the E. coli enzyme with regard to association with detergent micelles, affinity for ribosomes, and antigenicity.
Assuntos
Bacillus/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Cloretos , Compostos de Manganês , Fosfotransferases/isolamento & purificação , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Membrana Celular/enzimologia , Cromatografia de Afinidade , Eletroforese em Gel de Poliacrilamida , Cinética , Manganês/farmacologia , Peso Molecular , Octoxinol , Polietilenoglicóis/farmacologiaRESUMO
Membrane-associated phosphatidylserine synthase (CDP-diacylglycerol:L-serine O-phosphatidyltransferase, EC 2.7.8.8) was purified from the microsomal fraction of Saccharomyces cerevisiae strains S288C and VAL2C(YEpCHO1). VAL2C(YEpCHO1) contains a hybrid plasmid bearing the structural gene for phosphatidylserine synthase and overproduces the enzyme 6-7 fold (Letts, V. A., Klig, L. S., Bae-Lee, M., Carman, G. M., and Henry, S. A. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 7279-7283) compared to wild-type S288C. The purification procedure included Triton X-100 extraction of the microsomal membranes, CDP-diacylglycerol-Sepharose affinity chromatography, and DE-53 chromatography. The procedure yielded a preparation from each strain containing a major peptide band (Mr = 23,000) upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Phosphatidylserine synthase was dependent on manganese and Triton X-100 for maximum activity at pH 8.0. The apparent Km values for serine and CDP-diacylglycerol were 0.58 mM and 60 microM, respectively. Thioreactive agents inhibited enzyme activity. The enzyme was thermally labile above 40 degrees C. Results of isotopic exchange reactions between substrates and products suggest that the enzyme catalyzes a sequential Bi Bi reaction.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Membranas Intracelulares/enzimologia , Microssomos/enzimologia , Fosfatidilserinas/biossíntese , Fosfotransferases/isolamento & purificação , Saccharomyces cerevisiae/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Cátions Bivalentes , Cinética , Especificidade da EspécieRESUMO
The membrane-associated phospholipid biosynthetic enzyme cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:L-serine O-phosphatidyltransferase (phosphatidylserine synthase; EC 2.7.8.8) was partially purified 337-fold from a cell-free extract of the gram-positive pathogenic anaerobe Clostridium perfringens (ATCC 3624). The purification procedure included extraction from the cell envelope with the nonionic detergent Triton X-100, followed by affinity chromatography on cytidine 5'-diphosphate-diacylglycerol-Sepharose. When the partially purified enzyme was subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, two major bands were evident with apparent minimum molecular weights of 39,000 and 31,000. Activity of phosphatidylserine synthase was dependent on the addition of manganese ions (3 mM) and Triton X-100 (2.7 mM) for maximum activity. The rate of catalysis was maximal at 40 degrees C (with rapid thermal inactivation above this temperature), and the pH optimum was 8.5. The apparent Km values for cytidine 5'-diphosphate-diacylglycerol and L-serine were 0.24 and 0.26 mM, respectively. The synthetic (forward) reaction was favored, as indicated by an equilibrium constant of 82, and the energy of activation was found to be 18 kcal/mol (75,362 J/mol).
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Clostridium perfringens/enzimologia , Fosfotransferases/isolamento & purificação , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Diglicerídeos de Citidina Difosfato/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Manganês/farmacologia , Octoxinol , Polietilenoglicóis/farmacologia , Serina/metabolismo , TemperaturaRESUMO
When radiolabeled serine is incubated with a particulate fraction from Saccharomyces cerevisiae, radioactivity is incorporated initially into phosphatidylserine and gradually appears in phosphatidylethanolamine. Because decarboxylation of phosphatidylserine is blocked by hydroxylamine, phosphatidylserine synthase can be assayed separately. The yeast phosphatidylserine synthase activity 1) exhibits a divalent cation requirement; 2) is stimulated by exogenous CDP-diolein (apparent Km = 0.17 mM); 3) has an apparent Km = 4 mM for L-serine; 4) has a neutral pH optimum; 5) is inhibited by p-hydroxymercuribenzoate; and 6) is reversible in the presence of 5'-CMP, but not 2'-CMP, 3'-CMP, or 5'-AMP. The phospholipid-synthesizing activity is solubilized with Triton X-100 and the enzymatic parameters have been compared with the particulate form of the enzyme. Detergent extracts catalyze the conversion of exogenous purified [31P]CDP-diglyceride to [32P]phosphatidylserine in the presence of Mn2+ and L-serine. Enzyme preparations from cells grown in the presence of choline, that have reduced phospholipid methylation activity (Waechter, C. J., Steiner, M. R., and Lester, R. L. (1969) J. Biol. Chem. 244, 3419-3422), also have substantially less phosphatidylserine synthase activity compared to identical preparations grown in the absence of choline. When choline, phosphocholine, CDP-choline, and phosphatidylcholine are present in vitro, there is no direct inhibitory effect on phosphatidylserine synthase activity. While the inclusion of choline in the growth medium caused a significant reduction in phosphatidylserine synthase activity, it did not appreciably effect the apparent Km values for L-serine and CDP-diglyceride. These results are consistent with choline-grown cells containing less phosphatidylserine synthase activity because of lower amounts of enzyme present or perhaps less active enzyme due to covalent modification.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Colina/farmacologia , Fosfotransferases/metabolismo , Saccharomyces cerevisiae/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Cátions Bivalentes , Cinética , Proteínas de Membrana/isolamento & purificação , Saccharomyces cerevisiae/efeitos dos fármacosRESUMO
Membrane-associated cytidine 5'-diphospho-1,2-diacyl-sn-glycerol (CDP-diacylglycerol):L-serine O-phosphatidyltransferase (phosphatidylserine synthase, EC2.7.8.8.) and CDP-diacylglycerol:myo-inositol phosphatidyltransferase (phosphatidylinositol synthase, EC 2.7.8.11) were solubilized from the microsomal fraction of Saccharomyces cerevisiae. A variety of detergents were examined for their ability to release phosphatidylserine synthase and phosphatidylinositol synthase activities from the microsome fraction. Both enzymes were solubilized from the microsome fraction with Renex 690 in yield over 80% with increase to specific activity of 1.6-fold. Both solubilized enzymatic activities were dependent on manganese ions and Triton X-100 for maximum activity. The pH optimum for each reaction was 8.0. The apparent Km values for CDP-diacylglycerol and serine for the phosphatidylserine synthase reaction were 0.1 and 0.25 mM, respectively. The apparent Km values for CDP-diacylglycerol and inositol for the phosphatidylinositol synthase reaction were 70 microM and 0.1 mM, respectively. Thioreactive agents inhibited both enzymatic activities. Both solubilized enzymatic activities were thermally inactivated at temperatures above 30 degrees C.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Fosfotransferases/metabolismo , Saccharomyces cerevisiae/enzimologia , Transferases (Outros Grupos de Fosfato Substituídos) , CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferase , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Cátions Bivalentes/farmacologia , Monofosfato de Citidina/metabolismo , Detergentes/farmacologia , Concentração de Íons de Hidrogênio , Cinética , Microssomos/enzimologia , Fosfotransferases/isolamento & purificação , Solubilidade , TemperaturaRESUMO
The membrane fraction of exponentially growing cells of Saccharomyces cerevisiae was found to exhibit phosphatidylserine synthase activity. The enzyme was solubilized by Triton X-100 and chromatographed on a Sepharose 6B column. The enzyme had a pH optimum between 8.0 and 8.5. The apparent Km values for CDPdiacylglycerol and L-serine were 0.12 and 13 mM, respectively. Triton X-100 stimulated the enzyme. Mg2+ or Mn2+ was required for the activity. Ca2+ was inhibitory at relatively low concentrations. The enzyme was highly specific to L-serine. Labeling experiments showed that the enzyme synthesized phosphatidylserine by transferring the phosphatidyl moiety to L-serine. A mutant of S. cerevisiae defective in phosphatidylserine synthase was isolated. The strain required ethanolamine for its growth. Ethanolamine could be substituted by choline or high concentrations of L-serine. The mutant showed normal levels of CDPdiacylglycerol-inositol 3-phosphatidyltransferase and phosphatidylethanolamine methyltransferase activities.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Fosfotransferases/metabolismo , Saccharomyces cerevisiae/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/genética , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Cátions Bivalentes , Membrana Celular/enzimologia , Cinética , Mutação , Transferases/metabolismoAssuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Clostridium perfringens/enzimologia , Fosfotransferases/isolamento & purificação , Membrana Celular/efeitos dos fármacos , Membrana Celular/enzimologia , Clostridium perfringens/efeitos dos fármacos , Detergentes/farmacologia , Manganês/farmacologia , Octoxinol , Polietilenoglicóis/farmacologia , SolubilidadeRESUMO
The CDP-1,2-diacyl-sn-glycerol (CDP-diacylglycerol):L-serine O-phosphatidyltransferase (EC 2.7.8.8, phosphatidylserine synthase) of Escherichia coli is the first enzyme in the pathway committed to the biosynthesis of the major lipid in E. coli, phosphatidylethanolamine. The enzyme is unique among the phospholipid biosynthetic enzymes due to its high affinity for ribosomes in crude extracts. We report here investigations which define the nature of this in vitro affinity for ribosomes. Phosphatidylserine synthase can be dissociated from ribosomes in the presence of various inorganic salts at high ionic strength. Dissociation was also brought about by cellular levels of the polyamine spermidine. These results suggest the interaction of the enzyme with ribosomes in vitro is primarily ionic in nature, and polyamines may prevent this interaction in vivo. In the presence of nonionic detergent-lipid substrate mixed micelles under assay conditions the enzyme is also dissociated from ribosomes. Dissociation does not occur in the presence of detergent alone or in the presence of lipids which are not substrates or products of the enzyme. This dissociation by lipid substrate indicates the enzyme is not associated with ribosomes during catalysis.
Assuntos
CDPdiacilglicerol-Serina O-Fosfatidiltransferase/metabolismo , Escherichia coli/enzimologia , Fosfotransferases/metabolismo , Ribossomos/enzimologia , CDPdiacilglicerol-Serina O-Fosfatidiltransferase/isolamento & purificação , Concentração Osmolar , Ligação Proteica , Espermidina/farmacologiaRESUMO
Cytidine 5'-diphospho-1,2-diacyl-sn-glycerol (CDPdiglyceride):L-serine O-phosphatidyltransferase (EC 2.7.8.8, phosphatidylserine synthetase) is bound tightly to the ribosomes in crude extracts of Escherichia coli. After separation of the enzyme from the ribosomes by the method of Raetz and Kennedy (Raetz, C.R.H., and Kennedy, E.P. (1974), J. Biol. Chem. 249, 5038), we have purified the enzyme to 97% of homogenekty. The major portion of the overall 5500-fold purification was attained by substrate-specific elution from phosphocellulose using CDP-diglyceride in the presence of detergent. The purified enzyme migrated as a single band with an apparent minimum molecular weight of 54 000 when subjected to electrophoresis on polyacrylamide disc gels containing sodium dodecyl sulfate. The purified enzyme catalyzed exchange reactions between cytidine 5'- monophosphate (CMP) and CDP-diglyceride and between serine and phosphatidylserine. The enzyme also catalyzed the hydrolysis of CDP-diglyceride to form CMP and phosphatidic acid. dCDP-diglyceride was equivalent to CDP-diglyceride in all reactions catalyzed by the enzyme. In addition, the purified enzyme catalyzed the formation of phosphatidylglycerol or phosphatidylglycerophosphate at a very slow rate when serine was replaced as substrate by glycerol or sn-glycero-3-phosphate, respectively. These results suggest catalysis occurs via a ping-pong mechanism through the formation of a phosphatidyl-enzyme intermediate.