RESUMO
OBJECTIVE: The aim of the present study is to compare and assess the risk of periodontitis due to the presence of four putative periodontopathic bacteria viz., Eikenella corrodens, Campylobacter rectus, Prevotella intermedia and Prevotella nigrescens. To fulfil the above objective, polymerase Chain reaction using the primers targeting 16S rRNA gene of the bacterial species was performed with the subgingival plaque collected from the permanent first molars of type 1 diabetic children and age matched healthy children. RESULTS: The prevalence of periodontal pathogens in diabetic and healthy children was 6% and 16% for E. corrodens, 18% and 36% for C. rectus, 2% and 2% for P. intermedia, 4% and 0%, for P. nigrescens respectively. Statistically, significant difference was not observed for the prevalence of all the four periodontal pathogens between type 1 diabetic and healthy children (P = 1.00). The results of the present study thus reveal a negative correlation of type I diabetes to periodontitis in association to Eikenella corrodens, Campylobacter rectus, Prevotella intermedia and Prevotella nigrescens.
Assuntos
Campylobacter rectus/genética , Placa Dentária/microbiologia , Diabetes Mellitus Tipo 1/microbiologia , Eikenella corrodens/genética , Periodontite/microbiologia , Prevotella intermedia/genética , Prevotella nigrescens/genética , Adolescente , Técnicas de Tipagem Bacteriana , Campylobacter rectus/classificação , Campylobacter rectus/isolamento & purificação , Estudos de Casos e Controles , Criança , Placa Dentária/complicações , Placa Dentária/diagnóstico , Placa Dentária/patologia , Índice de Placa Dentária , Diabetes Mellitus Tipo 1/complicações , Diabetes Mellitus Tipo 1/diagnóstico , Diabetes Mellitus Tipo 1/patologia , Eikenella corrodens/classificação , Eikenella corrodens/isolamento & purificação , Feminino , Humanos , Masculino , Periodontite/complicações , Periodontite/diagnóstico , Periodontite/patologia , Prevotella intermedia/classificação , Prevotella intermedia/isolamento & purificação , Prevotella nigrescens/classificação , Prevotella nigrescens/isolamento & purificação , RNA Ribossômico 16S/genéticaRESUMO
Pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. To date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. We conducted a genome-wide association (GWA) study among 1,020 white participants of the Atherosclerosis Risk in Communities Study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom "checkerboard" DNA-DNA hybridization quantification of 8 periodontal pathogens was performed. We examined 3 traits: "high red" and "high orange" bacterial complexes, and "high" Aggregatibacter actinomycetemcomitans (Aa) colonization. Genotyping was performed on the Affymetrix 6.0 platform. Imputation to 2.5 million markers was based on HapMap II-CEU, and a multiple-test correction was applied (genome-wide threshold of p < 5 × 10(-8)). We detected no genome-wide significant signals. However, 13 loci, including KCNK1, FBXO38, UHRF2, IL33, RUNX2, TRPS1, CAMTA1, and VAMP3, provided suggestive evidence (p < 5 × 10(-6)) of association. All associations reported for "red" and "orange" complex microbiota, but not for Aa, had the same effect direction in a second sample of 123 African-American participants. None of these polymorphisms was associated with periodontitis diagnosis. Investigations replicating these findings may lead to an improved understanding of the complex nature of host-microbiome interactions that characterizes states of health and disease.
Assuntos
Periodontite Crônica/microbiologia , Metagenoma/genética , Periodonto/microbiologia , Aggregatibacter actinomycetemcomitans/classificação , Aggregatibacter actinomycetemcomitans/genética , Carga Bacteriana , Bacteroides/classificação , Bacteroides/genética , Proteínas de Ligação ao Cálcio/genética , Campylobacter rectus/classificação , Campylobacter rectus/genética , Subunidade alfa 1 de Fator de Ligação ao Core/genética , DNA Bacteriano/genética , Proteínas de Ligação a DNA/genética , Proteínas F-Box/genética , Feminino , Fusobacterium nucleatum/classificação , Fusobacterium nucleatum/genética , Predisposição Genética para Doença/genética , Estudo de Associação Genômica Ampla , Humanos , Interleucina-33 , Interleucinas/genética , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , Porphyromonas gingivalis/classificação , Porphyromonas gingivalis/genética , Canais de Potássio de Domínios Poros em Tandem/genética , Prevotella intermedia/classificação , Prevotella intermedia/genética , Prevotella nigrescens/classificação , Prevotella nigrescens/genética , Proteínas Repressoras , Transativadores/genética , Fatores de Transcrição/genética , Treponema denticola/classificação , Treponema denticola/genética , Ubiquitina-Proteína Ligases/genética , Proteína 3 Associada à Membrana da Vesícula/genética , Dedos de Zinco/genéticaRESUMO
BACKGROUND AND OBJECTIVE: The dog has been used extensively for experimental and microbiological studies on periodontitis and peri-implantitis without detailed knowledge about the predominant flora of the subgingival plaque. This study was designed to evaluate the predominant cultivable bacterial species in dogs and compare them phenotypically and genotypically with corresponding human species. MATERIAL AND METHODS: Four subgingival samples were taken from two upper premolars in each of six Labrador retrievers. The samples from each dog were processed for anaerobic culture. From the samples of each dog, the five or six predominating bacteria based on colony morphology were selected and pure cultured. Each of the strains was characterized by Gram stain, anaerobic/aerobic growth and API-ZYM test. Eighteen strains showing clear-cut phenotypic differences were further classified based on DNA sequencing technology. Cross-reactions of DNA probes from human and dog strains were also tested against a panel of both human and dog bacterial species. RESULTS: Thirty-one strains in the dogs were isolated and characterized. They represented 21 different species, of which six belonged to the genus Porphyromonas. No species was found consistently in the predominant flora of all six dogs. Porphyromonas crevioricanis and Fusobacterium canifelinum were the two most prevalent species in predominant flora in dogs. DNA probes from human and dog species cross-reacted to some extent with related strains from humans and dogs; however, distinct exceptions were found. CONCLUSION: The predominant cultural subgingival flora in dogs shows great similarities with the subgingival bacteria from humans at the genus level, but distinct differences at the species level; however, a genetic relatedness could be disclosed for most strains investigated.
Assuntos
Bactérias/classificação , Placa Dentária/microbiologia , Cães/microbiologia , Animais , Bactérias/genética , Técnicas Bacteriológicas , Bacteroides/classificação , Campylobacter/classificação , Campylobacter rectus/classificação , Sondas de DNA , DNA Bacteriano/análise , Modelos Animais de Doenças , Fusobacterium/classificação , Fusobacterium nucleatum/classificação , Genótipo , Bolsa Gengival/microbiologia , Gengivite/microbiologia , Humanos , Hibridização de Ácido Nucleico , Peptostreptococcus/classificação , Fenótipo , Porphyromonas/classificação , Porphyromonas endodontalis/classificação , Porphyromonas gingivalis/classificação , Prevotella intermedia/classificação , Análise de Sequência de DNA , Treponema denticola/classificaçãoRESUMO
We report the first fatal case of Campylobacter rectus infection due to a subdural empyema and ruptured mycotic intracranial aneurysm and two cases of limb-threatening C. rectus necrotizing soft tissue and bone infection and empyema thoracis that responded to amoxicillin-clavulanate and surgical debridement and drainage. All three strains were identified by 16S rRNA sequencing.
Assuntos
Infecções por Campylobacter/diagnóstico , Infecções por Campylobacter/patologia , Campylobacter rectus/isolamento & purificação , Adulto , Aneurisma Roto/complicações , Aneurisma Roto/diagnóstico , Aneurisma Roto/patologia , Infecções por Campylobacter/complicações , Infecções por Campylobacter/microbiologia , Campylobacter rectus/classificação , Campylobacter rectus/genética , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Empiema Pleural/diagnóstico , Empiema Pleural/microbiologia , Empiema Pleural/patologia , Empiema Subdural/diagnóstico , Empiema Subdural/microbiologia , Empiema Subdural/patologia , Evolução Fatal , Feminino , Humanos , Aneurisma Intracraniano/complicações , Aneurisma Intracraniano/diagnóstico , Aneurisma Intracraniano/patologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Micoses/complicações , Micoses/diagnóstico , Micoses/patologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. However, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. The aim of the present study was to examine such bone for bacteria using DNA-DNA hybridization. Compared to standard culture methods this technique enables the investigation of a vast number of bacteria in a fairly short time. Twelve deep medullary specimens from resected radionecrotic mandibles were studied. A multitude of bacterial species were detected, most of them anaerobic. Porphyromonas gingivalis was the most predominant organism, followed by Fusobacterium nucleatum subspecies polymorphum. All samples contained Actinomyces, Prevotella and F. nucleatum. The results of this study indicate that bacteria, particularly anaerobes, may play a more fundamental role in the pathophysiology of osteoradionecrosis than being merely surface contaminants.