Assuntos
Carcinoma de Brown-Pearce/tratamento farmacológico , Hematoporfirinas/uso terapêutico , Fotoquimioterapia/instrumentação , Neoplasias da Bexiga Urinária/tratamento farmacológico , Animais , Carcinoma in Situ/tratamento farmacológico , Derivado da Hematoporfirina , Lasers , Coelhos , Espalhamento de RadiaçãoRESUMO
Morphological observation was performed to see the effect of bronchial arterial infusion therapy (BAI) of mitomycin C (MMC) and also of the ischemia to the transplanted bronchial carcinoma. Single shot of 2mg/kg of MMC showed destructive changes on the tumor. Single shot of 3mg/kg and repeated administration of MMC brought relatively severe intra-arterial inflammatory changes such as intimal edema, thickening and proliferation which suggested ischemic effect on the tumor due to poor perfusion from stenosis or obstruction of the vessels. On the other hand a simple ligation of the artery also brought about more than moderate destructive changes in the tumor. Therefore, mechanisms of the effect of the BAI of MMC to the lung tumor might involve the secondary effect from ischemia in addition to the effect of MMC itself.
Assuntos
Antineoplásicos/administração & dosagem , Carcinoma Broncogênico/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Mitomicinas/administração & dosagem , Animais , Artérias Brônquicas/cirurgia , Carcinoma Broncogênico/irrigação sanguínea , Carcinoma de Brown-Pearce/tratamento farmacológico , Feminino , Humanos , Infusões Intra-Arteriais , Ligadura , Neoplasias Pulmonares/irrigação sanguínea , Masculino , Pessoa de Meia-Idade , Mitomicina , CoelhosRESUMO
Sustained release of 1,3-bis(2)chloroethyl)-1-nitrosourea (BCNU) via an episcleral implanted silicone device was used to treat Brown-Pearce epithelioma in the anterior chamber of rabbit eyes. One group of test eyes received BCNU dissolved in sesame oil; a second group received BCNU in pure ethanol. Control eyes received only the diluent, sesame oil or pure ethanol. The effectiveness of the various dosages and diluents was compared by clinical observation, by weight of the enucleated eyes, and by histopathologic examination. Sustained release of BCNU via an episcleral implanted silicone device delayed the growth of Brown-Pearce epithelioma in rabbit eyes of both test groups. The most effective action resulted from administration of BCNU in pure ethanol.
Assuntos
Câmara Anterior , Carcinoma de Brown-Pearce/tratamento farmacológico , Carmustina/administração & dosagem , Neoplasias Oculares/tratamento farmacológico , Animais , Implantes de Medicamento , Etanol , Feminino , Transplante de Neoplasias , Veículos Farmacêuticos , Coelhos , Óleo de Gergelim , Elastômeros de SiliconeRESUMO
Transplantable Brown-Pearce carcinoma was adapted successfully in the rabbit anterior chamber. Regression of tumor growth was attained on tri-weekly perfusion of the AC with 10 micromolar of methotrexate. Tumor cyclic nucleotide phosphodiesterase (PDE) and protein activator were found to be markedly depressed during the course of chemotherapy and the PDE cAMP/cGMP ratio was similarly altered. Corroborative light and electron-microscopic studies showed specific alterations of intracellular organelles in relation to MTX and tumor cell death. These findings suggest that metabolic pathways of cyclic nucleotides are important biochemical modulators of neoplastic cells. The method of intraocular perfusion precludes systemic toxic effects and avoids compromising the animals' immunocompetence.
Assuntos
Carcinoma de Brown-Pearce/tratamento farmacológico , Neoplasias Oculares/tratamento farmacológico , Metotrexato/uso terapêutico , Animais , Câmara Anterior/cirurgia , Carcinoma de Brown-Pearce/enzimologia , Carcinoma de Brown-Pearce/ultraestrutura , Quimioterapia do Câncer por Perfusão Regional , Neoplasias Oculares/enzimologia , Neoplasias Oculares/ultraestrutura , Feminino , Masculino , Microscopia Eletrônica , Transplante de Neoplasias , Neoplasias Experimentais/tratamento farmacológico , Diester Fosfórico Hidrolases/análise , Coelhos , Tetra-Hidrofolato Desidrogenase/análiseRESUMO
Two anticancer agents, one lipophilic, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), and one hydrophilic, 5-(3,3-dimethyl-1-triazeno) imidazol-4-carboxamide (DTIC), were used to treat Brown-Pearce epithelioma in the anterior chamber of rabbit eyes. The BCNU test animals were divided into three groups: one treated by direct injection of the drug into the subconjuntival space or the anterior chamber, the second by both direct injections and intravenous administration, and the third by intravenous injection alone. The DTIC test animals were treated with only local injection into the subconjunctival space or anterior chamber. Dosage, delivery system, and effectiveness were compared following clinical observation and histopathologic examination. Direct delivery of BCNU or DTIC in subconjuntival space or anterior chamber delayed the growth of Brown-Pearce epithelioma in rabbit eye. The effectiveness of this treatment was significnatly enhanced by combining direct injection with systemic administration of a lower dose of BCNU.
Assuntos
Câmara Anterior , Carmustina/administração & dosagem , Dacarbazina/administração & dosagem , Neoplasias Oculares/tratamento farmacológico , Triazenos/administração & dosagem , Animais , Câmara Anterior/patologia , Carcinoma de Brown-Pearce/tratamento farmacológico , Carcinoma de Brown-Pearce/patologia , Túnica Conjuntiva , Neoplasias Oculares/patologia , Feminino , Injeções , Injeções Intravenosas , CoelhosAssuntos
Levamisol/uso terapêutico , Neoplasias/tratamento farmacológico , Animais , Dor nas Costas/induzido quimicamente , Carcinoma de Brown-Pearce/tratamento farmacológico , Ensaios Clínicos como Assunto , Cricetinae , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Gastroenteropatias/induzido quimicamente , Humanos , Leucemia Experimental/tratamento farmacológico , Levamisol/efeitos adversos , Levamisol/imunologia , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Metástase Neoplásica/tratamento farmacológico , Transplante de Neoplasias , Neoplasias Experimentais/tratamento farmacológico , Doenças do Sistema Nervoso/induzido quimicamente , Placebos , Ratos , Sarcoma/tratamento farmacológico , Manifestações CutâneasRESUMO
All known morphological signs of cell dystrophy are systematized. Each cell was described according to multiple morphological signs (100 cells in each specimen); 20 000 tumor cells in carcinomatous exudates of patients with different localizations of malignant tumor were studied during the course of treatment, and 9 100 cells of experimental Brown-Pearce tumors were subjected to various effects. There were revealed more informative signs for judging the efficacy of treatment in patients. Based on more informative signs, which proved to be nucleoli signs, the indices for nucleoli were elaborated to determine the degree of tumor cells injury and to estimate the efficacy of treatment of patients by means of alkylating antiblastic substances. A semiautomatic set "Cytoclassificator" was employed for the study.