Leaf and shoot apical meristem transcriptomes of quinoa (Chenopodium quinoa Willd.) in response to photoperiod and plant development.

Understanding the regulation of flowering time is crucial for adaptation of crops to new environment. In this study, we examined the timing of floral transition and analysed transcriptomes in leaf and shoot apical meristems of photoperiod-sensitive and -insensitive quinoa accessions. Histological analysis showed that floral transition in quinoa initiates 2-3 weeks after sowing. We found four groups of differentially expressed genes in quinoa genome that responded to plant development and floral transition: (i) 222 genes responsive to photoperiod in leaves, (ii) 1812 genes differentially expressed between accessions under long-day conditions in leaves, (iii) 57 genes responding to developmental changes under short-day conditions in leaves and (iv) 911 genes responding to floral transition within the shoot apical meristem. Interestingly, among numerous candidate genes, two putative FT orthologs together with other genes (e.g. SOC1, COL, AP1) were previously reported as key regulators of flowering time in other species. Additionally, we used coexpression networks to associate novel transcripts to a putative biological process based on the annotated genes within the same coexpression cluster. The candidate genes in this study would benefit quinoa breeding by identifying and integrating their beneficial haplotypes in crossing programs to develop adapted cultivars to diverse environmental conditions.

Salt-tolerance mechanisms in quinoa: Is glycinebetaine the missing piece of the puzzle?

Salinization of arable land has been progressively increasing, which, along with the effects of climate change, poses a serious risk to food production. Quinoa is a halophyte species that grows and is productive in highly saline soils. This study addresses the mechanisms of response and adaptation to high salinity. We show that the differential distribution of sodium in plants depends on the variety, observing that varieties such as Pandela Rosada limit the passage transit of sodium to the aerial part of the plant, a mechanism that seems to be regulated by sodium transporters such as HKT1s or SOS1. Like other halophytes of the Amaranthaceae family, quinoa plants have salt glands (bladder cells), which have been reported to play an important role in salt tolerance. However, our study shows that the contribution of bladder glands to salt accumulation is rather low. The 1H-NMR metabolome study of quinoa subjected to salt stress showed important modifications in the contents of amino acids, sugars, organic acids, and quaternary ammonium compounds (glycinebetaine). The compound with a higher presence was glycinebetaine, which makes up 6% of the leaf dry matter under saline conditions. Our findings suggest that glycinebetaine can act as an osmolyte and/or osmoprotectant, facilitating plant development under high saline ambient.

Quinoa panicles contribute to carbon assimilation and are more tolerant to salt stress than leaves.

Contribution of inflorescences to seed filling have attracted great attention given the resilience of this photosynthetic organ to stressful conditions. However, studies have been almost exclusively focused to small grain cereals. In this study, we aimed to explore these responses in quinoa, as a climate resilient seed crop of elevated economic and nutritious potential. We compared the physiological and metabolic performance of panicles and leaves of two quinoa cultivars growing under contrasting salinity levels. Plant growth, photosynthetic and transpiratory gas exchange and chlorophyll fluorescence were monitored in inflorescences and leaves throughout the experiment. At flowering stage, young and mature leaves and panicles were sampled for key metabolic markers related to carbon, nitrogen and secondary metabolisms. When subjected to salt stress, panicles showed attenuated declines on photosynthesis, water use, pigments, amino acids, and protein levels as compared to leaves. In fact, the assimilation rates, together with a high hexose content evidenced an active photosynthetic role of the panicle under optimal and salt stress conditions. Moreover, we also found significant genotypic variability for physiological and metabolic traits of panicles and leaves, which emphasizes the study of genotype-dependent stress responses at the whole plant level. We conclude that quinoa panicles are less affected by salt stress than leaves, which encourages further research and exploitation of this organ for crop improvement and stress resilience considering the high natural diversity.

Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings.

BACKGROUND: Quinoa (Chenopodium quinoa Willd.) originates in high altitude areas, such as the Andes, and has some inherent characteristics of cold, drought, and salinity tolerance, but is sensitive to high temperature. RESULTS: To gain insight into the response mechanism of quinoa to high temperature stress, we conducted an extensive targeted metabolomic study of two cultivars, Dianli-3101 and Dianli-3051, along with a combined transcriptome analysis. A total of 794 metabolites and 54,200 genes were detected, in which the genes related to photosynthesis were found down-regulated at high temperatures, and two metabolites, lipids and flavonoids, showed the largest changes in differential accumulation. Further analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and transcription factors revealed that quinoa inhibits photosynthesis at high temperatures, and the possible strategies being used for high temperature stress management are regulation of heat stress transcription factors (HSFs) to obtain heat tolerance, and regulation of purine metabolism to enhance stress signals for rapid response to high temperature stress. The tolerant genotype could have an enhanced response through lower purine levels. The induction of the stress response could be mediated by HSF transcription factors. The results of this study may provide theoretical references for understanding the response mechanism of quinoa to high temperature stress, and for screening potential high temperature tolerant target genes and high temperature tolerant strains. CONCLUSIONS: These findings reveal the regulation of the transcription factor family HSF and the purinergic pathway in response to high temperature stress to improve quinoa varieties with high temperature tolerance.

Photosynthesis is not the unique useful trait for discriminating salt tolerance capacity between sensitive and tolerant quinoa varieties.

MAIN CONCLUSION: Growth was not strictly linked to photosynthesis performance under salinity conditions in quinoa. Other key traits, which were varieties-specific, rather than photosynthesis explained better growth performance. Phenotyping for salinity stress tolerance in quinoa is of great interest to select traits contributing to overall salinity tolerance and to understand the response mechanisms to salinity at a whole plant level. The objective of this work was to dissect the responses of specific traits and analyse relations between these traits to better understand growth response under salinity conditions in quinoa. Growth response to salinity was mostly related to differences in basal values of biomass, being reduced the most in plants with higher basal biomass. Regarding the relationship between growth and specific traits, in Puno variety, better photosynthetic performance was related to a better maintenance of growth. Nevertheless, in the rest of the varieties other traits rather than photosynthesis could better explain growth response. In this way, the development of succulence in F-16 and Collana varieties, also the osmotic adjustment but in smaller dimensions in Pasankalla, Marisma and S-15-15 helped to maintain better growth. Besides, smaller increases of Cl- could have caused a limited nitrate uptake reducing more growth in Vikinga. Ascorbate was considered a key trait as a noticeable fall of it was also related to higher reductions in growth in Titicaca. These results suggest that, due to the genetic variability of quinoa and the complexity of salinity tolerance, no unique and specific traits should be taken into consideration when using phenotyping for analysing salinity tolerance in quinoa.

Transcriptomic and metabolomic landscape of quinoa during seed germination.

BACKGROUND: Quinoa (Chenopodium quinoa), a dicotyledonous species native to Andean region, is an emerging crop worldwide nowadays due to its high nutritional value and resistance to extreme abiotic stresses. Although it is well known that seed germination is an important and multiple physiological process, the network regulation of quinoa seed germination is largely unknown. RESULTS: Here, we performed transcriptomic study in five stages during transition from quinoa dry seed to seedling. Together with the GC-MS based metabolome analysis, we found that seed metabolism is reprogrammed with significant alteration of multiple phytohormones (especially abscisic acid) and other nutrients during the elongation of radicels. Cell-wall remodeling is another main active process happening in the early period of quinoa seed germination. Photosynthesis was fully activated at the final stage, promoting the biosynthesis of amino acids and protein to allow seedling growth. The multi-omics analysis revealed global changes in metabolic pathways and phenotype during quinoa seed germination. CONCLUSION: The transcriptomic and metabolomic landscape depicted here pave ways for further gene function elucidation and quinoa development in the future.

Haplotype variations of major flowering time genes in quinoa unveil their role in the adaptation to different environmental conditions.

Response to photoperiod is of major importance in crop production. It defines the adaptation of plants to local environments. Quinoa is a short-day plant which had been domesticated in the Andeans regions. We wanted to understand the adaptation to long-day conditions by studying orthologues of two major flowering time regulators of Arabidopsis, FLOWERING LOCUS T (FT) and CONSTANS (CO) in quinoa accessions with contrasting photoperiod response. By searching the quinoa reference genome sequence, we identified 24 FT and six CO homologs. CqFT genes displayed remarkably different expression patterns between long- and short-day conditions, whereas the influence of the photoperiod on CqCOL expressions was moderate. Cultivation of 276 quinoa accessions under short- and long-day conditions revealed great differences in photoperiod sensitivity. After sequencing their genomes, we identified large sequence variations in 12 flowering time genes. We found non-random distribution of haplotypes across accessions from different geographical origins, highlighting the role of CqFT and CqCOL genes in the adaptation to different day-length conditions. We identified five haplotypes causing early flowering under long days. This study provides assets for quinoa breeding because superior haplotypes can be assembled in a predictive breeding approach to produce well-adapted early flowering lines under long-day photoperiods.

Photosynthetic performance of quinoa (Chenopodium quinoa Willd.) after exposure to a gradual drought stress followed by a recovery period.

Drought is an abiotic scourge, one of the major environmental stress factors that adversely affect plant growth and photosynthesis machinery through a disruption of cell organelles, arrangement thylakoid membranes and the electron transport chain. Herein, we probed the effect of drought stress on photosynthetic performance of Chenopodium quinoa Willd. Beforehand, plants were subjected to water deficit (as 15% Field Capacity, FC) for one (D-1W) or two weeks (D-2W), and were then re-watered at 95% FC for 2 weeks. Light and electron microscopy analysis of leaves showed no apparent changes in mesophyll cell organization and chloroplast ultrastructure after one week of drought stress, while a swelling of thylakoids and starch accumulation were observed after the prolonged drought (D-2W). The latter induced a decrease in both PSI and PSII quantum yields which was accompanied by an increase in F0 (minimum fluorescence) and a decline in Fm (maximum fluorescence). Drought stress influenced the fluorescence transients, where the major changes at the OJIP prompt FI level were detected in the OJ and IP phases. Prolonged drought induced a decrease in chl a fluorescence at IP phase which was readjusted and established back after re-watering and even more an increase was observed after 2 weeks of recovery. The maximum quantum yield of primary photochemistry (φPo) was unaffected by the different drought stress regimes. Drought induced an increase in the ABS/RC and DI0/RC ratios which was concurrent to a stable φPo (maximum quantum yield of PSII primary photochemistry). A substantial decrease in PI(ABS) was detected especially, during severe drought stress (D-2W) suggesting a drop in the PSII efficiency and the level of electron transport through the plastoquinone pool (PQ pool) towards oxidized PSI RCs (P700+). The immunoblot analysis of the main PSII proteins revealed considerable changes in the D1, D2, CP47, OEC, PsbQ and LHCII proteins under drought. These changes depend on the stress duration and recovery period. The main message of this investigation is the elevated recovery capacities of PSII and PSI photochemical activities after re-watering.

Transcriptome analysis and differential gene expression profiling of two contrasting quinoa genotypes in response to salt stress.

BACKGROUND: Soil salinity is one of the major abiotic stress factors that affect crop growth and yield, which seriously restricts the sustainable development of agriculture. Quinoa is considered as one of the most promising crops in the future for its high nutrition value and strong adaptability to extreme weather and soil conditions. However, the molecular mechanisms underlying the adaptive response to salinity stress of quinoa remain poorly understood. To identify candidate genes related to salt tolerance, we performed reference-guided assembly and compared the gene expression in roots treated with 300 mM NaCl for 0, 0.5, 2, and 24 h of two contrasting quinoa genotypes differing in salt tolerance. RESULTS: The salt-tolerant (ST) genotype displayed higher seed germination rate and plant survival rate, and stronger seedling growth potential as well than the salt-sensitive (SS) genotype under salt stress. An average of 38,510,203 high-quality clean reads were generated. Significant Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified to deeper understand the differential response. Transcriptome analysis indicated that salt-responsive genes in quinoa were mainly related to biosynthesis of secondary metabolites, alpha-Linolenic acid metabolism, plant hormone signal transduction, and metabolic pathways. Moreover, several pathways were significantly enriched amongst the differentially expressed genes (DEGs) in ST genotypes, such as phenylpropanoid biosynthesis, plant-pathogen interaction, isoquinoline alkaloid biosynthesis, and tyrosine metabolism. One hundred seventeen DEGs were common to various stages of both genotypes, identified as core salt-responsive genes, including some transcription factor members, like MYB, WRKY and NAC, and some plant hormone signal transduction related genes, like PYL, PP2C and TIFY10A, which play an important role in the adaptation to salt conditions of this species. The expression patterns of 21 DEGs were detected by quantitative real-time PCR (qRT-PCR) and confirmed the reliability of the RNA-Seq results. CONCLUSIONS: We identified candidate genes involved in salt tolerance in quinoa, as well as some DEGs exclusively expressed in ST genotype. The DEGs common to both genotypes under salt stress may be the key genes for quinoa to adapt to salinity environment. These candidate genes regulate salt tolerance primarily by participating in reactive oxygen species (ROS) scavenging system, protein kinases biosynthesis, plant hormone signal transduction and other important biological processes. These findings provide theoretical basis for further understanding the regulation mechanism underlying salt tolerance network of quinoa, as well establish foundation for improving its tolerance to salinity in future breeding programs.

The genotype-dependent phenotypic landscape of quinoa in salt tolerance and key growth traits.

Cultivation of quinoa (Chenopodium quinoa), an annual pseudocereal crop that originated in the Andes, is spreading globally. Because quinoa is highly nutritious and resistant to multiple abiotic stresses, it is emerging as a valuable crop to provide food and nutrition security worldwide. However, molecular analyses have been hindered by the genetic heterogeneity resulting from partial outcrossing. In this study, we generated 136 inbred quinoa lines as a basis for the molecular identification and characterization of gene functions in quinoa through genotyping and phenotyping. Following genotyping-by-sequencing analysis of the inbred lines, we selected 5,753 single-nucleotide polymorphisms (SNPs) in the quinoa genome. Based on these SNPs, we show that our quinoa inbred lines fall into three genetic sub-populations. Moreover, we measured phenotypes, such as salt tolerance and key growth traits in the inbred quinoa lines and generated a heatmap that provides a succinct overview of the genotype-phenotype relationship between inbred quinoa lines. We also demonstrate that, in contrast to northern highland lines, most lowland and southern highland lines can germinate even under high salinity conditions. These findings provide a basis for the molecular elucidation and genetic improvement of quinoa and improve our understanding of the evolutionary process underlying quinoa domestication.

N metabolism performance in Chenopodium quinoa subjected to drought or salt stress conditions.

Currently it is estimated that the 20% of total cultivated land is affected by salt. Besides, drought events will increase worldwide. These factors are affecting plant growth and crop production compromising food security. Within this context, quinoa (Chenopodium quinoa) is becoming an alternative pseudocereal for food supply due to its capacity to grow under harsh environmental conditions. Besides, it is being proposed as key model species to study the physiological processes that permit this tolerance, although how N metabolism responds has been barely studied. This paper addresses, on one hand, the response of quinoa's N metabolism (N uptake, translocation, reduction and assimilation) under the forthcoming climatic conditions and, on the other hand, the comparison of the effects of both stresses when plants have similar relative water content and photosynthetic rates. Under mild salt stress (120 and 240 mM NaCl) N assimilation is not affected, while the N uptake is favored. Under severe salt stress (500 mM NaCl), N uptake is reduced, decreasing leaf nitrate and protein concentration; nevertheless, leaf free amino acids are maintained -to perform osmotic adjustment-. N uptake rate is more affected under drought than under severe salt; furthermore, under severe salt stress, quinoa allocates more nitrogen to roots to finely regulate NO3- and Cl- uptake, while under drought it allocates more to leaves to ensure photosynthesis. These results indicate that quinoa's N metabolism is tolerant to drought and salt stress, although the strategies of this species for coping with the aforementioned stresses are different.

Cross-talk between nitric oxide, hydrogen peroxide and calcium in salt-stressed Chenopodium quinoa Willd. At seed germination stage.

Seed germination is critical for successful crop production and this growth stage can be very sensitive to salt stress depending on the plant's tolerance mechanisms. The pretreatment of Chenopodium quinoa (quinoa) seeds with CaCl2, H2O2 and sodium nitroprusside (SNP) limited the adverse effect of salt stress on seed germination. The pre-treated seeds showed a significant increase in germination rate, relative germination rate and germination index while the mean germination time was significantly reduced under both optimal and stress conditions. In parallel with seed germination, the negative effect of salt stress on the activity of α-amylase and ß-amylase was reduced in pre-treated seeds. The amylase enzymes are responsible for starch hydrolysis, so the reduction of amylase activity by salt stress resulted in higher starch content in the seeds and lower concentrations of water-soluble sugars such as glucose. Pretreatment stimulated amylase activity resulting in starch breakdown and increased content of water-soluble sugars in the salt-stressed seeds. Protein and amino acid contents were significantly enhanced in salt-stressed seeds, which were highlighted in pre-treated seeds. The findings of this study demonstrate that pretreatment of quinoa seeds with CaCl2, H2O2 and SNP at 5, 5 and 0.2 mM, respectively, concentration to achieve rapid germination at high levels under optimal and salt-stress conditions.

Understanding the role of root-related traits in salinity tolerance of quinoa accessions with contrasting epidermal bladder cell patterning.

MAIN CONCLUSION: To compensate for the lack of capacity for external salt storage in the epidermal bladder cells, quinoa plants employ tissue-tolerance traits, to confer salinity stress tolerance. Our previous studies indicated that sequestration of toxic Na+ and Cl- ions into epidermal bladder cells (EBCs) is an efficient mechanism conferring salinity tolerance in quinoa. However, some halophytes do not develop EBCs but still possess superior salinity tolerance. To elucidate the possible compensation mechanism(s) underlying superior salinity tolerance in the absence of the external salt storage capacity, we have selected four quinoa accessions with contrasting patterns of EBC development. Whole-plant physiological and electrophysiological characteristics were assessed after 2 days and 3 weeks of 400 mM NaCl stress. Both accessions with low EBC volume utilised Na+ exclusion at the root level and could maintain low Na+ concentration in leaves to compensate for the inability to sequester Na+ load in EBC. These conclusions were further confirmed by electrophysiological experiments showing higher Na+ efflux from roots of these varieties (measured by a non-invasive microelectrode MIFE technique) as compared to accessions with high EBC volume. Furthermore, accessions with low EBC volume had significantly higher K+ concentration in their leaves upon long-term salinity exposures compared to plants with high EBC sequestration ability, suggesting that the ability to maintain high K+ content in the leaf mesophyll was as another important compensation mechanism.

Nutritional, Microbial, and Allergenic Changes during the Fermentation of Cashew 'Cheese' Product Using a Quinoa-Based Rejuvelac Starter Culture.

Fermentation has been applied to a multitude of food types for preservation and product enhancing characteristics. Interest in the microbiome and healthy foods makes it important to understand the microbial processes involved in fermentation. This is particularly the case for products such as fermented cashew (Anacardium occidentale). We hereby describe the characterisation of cashew samples throughout an entire fermentation production process, starting at the quinoa starter inoculum (rejuvelac). The viable bacterial count was 108 -109 colony forming units/g. The nutritional composition changed marginally with regards to fats, carbohydrates, vitamins, and minerals. The rejuvelac starter culture was predominated by Pediococcus and Weissella genera. The 'brie' and 'blue' cashew products became dominated by Lactococcus, Pediococcus, and Weissella genera as the fermentation progressed. Cashew allergenicity was found to significantly decrease with fermentation of all the end-product types. For consumers concerned about allergic reactions to cashew nuts, these results suggested that a safer option is for products to be made by fermentation.

Comparative physiological and biochemical mechanisms of salt tolerance in five contrasting highland quinoa cultivars.

BACKGROUND: Chenopodium quinoa Willd., a halophytic crop, shows great variability among different genotypes in response to salt. To investigate the salinity tolerance mechanisms, five contrasting quinoa cultivars belonging to highland ecotype were compared for their seed germination (under 0, 100 and 400 mM NaCl) and seedling's responses under five salinity levels (0, 100, 200, 300 and 400 mM NaCl). RESULTS: Substantial variations were found in plant size (biomass) and overall salinity tolerance (plant biomass in salt treatment as % of control) among the different quinoa cultivars. Plant salinity tolerance was negatively associated with plant size, especially at lower salinity levels (< 300 mM NaCl), but salt tolerance between seed germination and seedling growth was not closely correlated. Except for shoot/root ratio, all measured plant traits responded to salt in a genotype-specific way. Salt stress resulted in decreased plant height, leaf area, root length, and root/shoot ratio in each cultivar. With increasing salinity levels, leaf superoxide dismutase (SOD) activity and lipid peroxidation generally increased, but catalase (CAT) and peroxidase (POD) activities showed non-linear patterns. Organic solutes (soluble sugar, proline and protein) accumulated in leaves, whereas inorganic ion (Na+ and K+) increased but K+/Na+ decreased in both leaves and roots. Across different salinity levels and cultivars, without close relationships with antioxidant enzyme activities (SOD, POD, or CAT), salinity tolerance was significantly negatively correlated with organic solute and malondialdehyde contents in leaves and inorganic ion contents in leaves or roots (except for root K+ content), but positively correlated with K+/Na+ ratio in leaves or roots. CONCLUSION: Our results indicate that leaf osmoregulation, K+ retention, Na+ exclusion, and ion homeostasis are the main physiological mechanisms conferring salinity tolerance of these cultivars, rather than the regulations of leaf antioxidative ability. As an index of salinity tolerance, K+/Na+ ratio in leaves or roots can be used for the selective breeding of highland quinoa cultivars.

Comparing Kinetics of Xylem Ion Loading and Its Regulation in Halophytes and Glycophytes.

Although control of xylem ion loading is essential to confer salinity stress tolerance, specific details behind this process remain elusive. In this work, we compared the kinetics of xylem Na+ and K+ loading between two halophytes (Atriplex lentiformis and quinoa) and two glycophyte (pea and beans) species, to understand the mechanistic basis of the above process. Halophyte plants had high initial amounts of Na+ in the leaf, even when grown in the absence of the salt stress. This was matched by 7-fold higher xylem sap Na+ concentration compared with glycophyte plants. Upon salinity exposure, the xylem sap Na+ concentration increased rapidly but transiently in halophytes, while in glycophytes this increase was much delayed. Electrophysiological experiments using the microelectrode ion flux measuring technique showed that glycophyte plants tend to re-absorb Na+ back into the stele, thus reducing xylem Na+ load at the early stages of salinity exposure. The halophyte plants, however, were capable to release Na+ even in the presence of high Na+ concentrations in the xylem. The presence of hydrogen peroxide (H2O2) [mimicking NaCl stress-induced reactive oxygen species (ROS) accumulation in the root] caused a massive Na+ and Ca2+ uptake into the root stele, while triggering a substantial K+ efflux from the cytosol into apoplast in glycophyte but not halophytes species. The peak in H2O2 production was achieved faster in halophytes (30 min vs 4 h) and was attributed to the increased transcript levels of RbohE. Pharmacological data suggested that non-selective cation channels are unlikely to play a major role in ROS-mediated xylem Na+ loading.

Soil drenching of paclobutrazol: An efficient way to improve quinoa performance under salinity.

Salinity extent and severity is rising because of poor management practices on agricultural lands, possibility lies to grow salt-tolerant crops with better management techniques. Therefore, a highly nutritive salt-tolerant crop quinoa with immense potential to contribute for future food security was selected for this investigation. Soil drenching of paclobutrazol (PBZ; 20 mg l-1 ) was used to understand the ionic relations, gaseous exchange characteristics, oxidative defense system and yield under saline conditions (400 mM NaCl) including normal (0 mM NaCl) and no PBZ (0 mg l-1 ) as controls. The results revealed that salinity stress reduced the growth and yield of quinoa through perturbing ionic homeostasis with the consequences of overproduction of reactive oxygen species (ROS), oxidative damages and reduced photosynthesis. PBZ improved the quinoa performance through regulation of ionic homeostasis by decreasing Na+ , Cl- , while improving K+ , Mg2+ and Ca2+ concentration. It also enhanced the antioxidative system including ascorbic acid, phenylalanine ammonia-lyase, polyphenol oxidase and glutathione peroxidase, which scavenged the ROS (H2 O2 and O2 •- ) and lowered the oxidative damages (malondialdehyde level) under salinity in roots and more specifically in leaf tissues. The photosynthetic rate and stomatal conductance consequently improved (16 and 21%, respectively) in salt-stressed quinoa PBZ-treated compared to the non-treated ones and contributed to the improvement of panicle length (33%), 100-grain weight (8%) and grain yield (38%). Therefore, PBZ can be opted as a shotgun approach to improve quinoa performance and other crops under high saline conditions.

Influencia del consumo de quinua (Chenopodium quinoa Willd. ) sobre la acumulación del tejido adiposo y actividad antioxidante en tejidos de ratas obesas / Influence of the consumption of quinoa (Chenopodium quinoa Willd.) on the accumulation of adipose tissue and antioxidant activity in tissues of obese rats

Se han encontrado compuestos bioactivos en frutas y vegetales con efecto favorable en la prevención de enfermedades crónicas como cáncer y Alzheimer, que también están presentes en la quinua. Son necesarios realizar estudios para evaluar el potencial benéfico in vivo. El objetivo fue determinar la influencia del consumo de tres variedades de quinua (Chenopodium quinoa Willd.) sobre la acumulación de tejido adiposo y actividad antioxidante en tejidos de ratas obesas. 42 ratas obesas Holtzman fueron divididas en siete grupos. Con excepción del control, los otros grupos fueron alimentadas, durante 23 días, con dietas obesogénicas conteniendo 20% quinua variedad Altiplano, Pasankalla o Negra Collana, procesadas por cocción o tostado. Al finalizar el período de alimentación, la grasa visceral (GV) y abdominal (GA) fueron pesadas y se extrajo muestras de hígado e intestino delgado (ID) siendo almacenadas (­20°C) para posteriores análisis de actividad antioxidante. Se midieron capacidad antioxidante y contenido de polifenoles totales en quinuas procesadas. Se aplicó ANOVA utilizando el modelo lineal generalizado para diseño completamente randomizado con arreglo factorial 3x2+Control; y test de Fisher mediante el programa Minitabv.17.1.0. La GV, GA y peroxidación lipídica del ID no fueron afectadas significativamente (P>0.05) por las dietas experimentales; sin embargo, la peroxidación lipídica en el hígado de ratas que consumieron dietas con quinua fue significativamente menor (P<0.05) que el control. En conclusión, la alimentación de ratas obesas con dietas que contienen variedades de quinua procesada no afecta la acumulación de GV y GA; sin embargo, reduce la peroxidación lipídica en el hígado(AU)

Bioactive compounds with favorable effect in the prevention of chronic diseases such as cancer and Alzheimer are found in fruits and vegetables and are also present in quinoa. Studies are needed to evaluate the potential benefits in vivo. The objective was to determine the influence of the consumption of three varieties of quinoa (Chenopodium quinoa Willd.) on the accumulation of adipose tissue and antioxidant activity in tissues of obese rats. Forty two obese Holtzman rats were divided into seven groups. With exception of the control, the other groups were fed, during 23 days, with obesogenic diets containing 20% quinoa Altiplano, Pasankalla or Negra Collana, processed by cooking or roasting. At the end of the feeding period, visceral (VF) and abdominal fat (AF) were weighed and samples of liver and small intestine (SI) were extracted and stored (-20°C) for further analysis of antioxidant activity. Antioxidant capacity and total polyphenol content were measured in the processed quinoa. Applied ANOVA using the generalized linear model for a completely randomized design with a factorial arrangement 3x2+Control; and Fisher's test through the statistical program Minitab v.17.1.0. VF, AF and lipid peroxidation of SI were not significantly influenced (P>0.05) by the experimental diets; however, lipid peroxidation in the liver of rats consuming quinoa containing-diets was significantly lower (P <0.05) than control group. In conclusion, feeding obese rats with quinoa containing-diets do not affect the accumulation of VF and AF; however, it reduce lipid peroxidation in the liver(AU)

Physiological effects of short acute UVB treatments in Chenopodium quinoa Willd.

Increased ultraviolet B (UVB) radiation due to global change can affect plant growth and metabolism. Here, we evaluated the capacity of quinoa to resist under short acute UVB irradiation. Quinoa was daily exposed for 30 or 60 min to 1.69 W m-2 UVB. The results showed that 30 min exposure in 9 d-course did not cause severe alterations on photosynthetic pigments and flavonoids, but a significant increase of antioxidant capacity was observed. Otherwise, 60 min UVB in 5 d-course reduced almost all these parameters except for an increase in the de-epoxidation of xanthophyll cycle pigments and led to the death of the plants. Further studies of gas exchange and fluorescence measurements showed that 30 min UVB dramatically decrease stomatal conductance, probably associated to reactive oxygen species (ROS) production. Inhibition of photosynthetic electron transport was also observed, which could be a response to reduce ROS. Otherwise, irreversible damage to the photosynthetic apparatus was found with 60 min UVB probably due to severe ROS overproduction that decompensates the redox balance inducing UVB non-specific signaling. Moreover, 60 min UVB compromised Rubisco carboxylase activity and photosynthetic electron transport. Overall, these data suggest that quinoa modulates different response mechanisms depending on the UVB irradiation dosage.

Genotypic differences in agro-physiological, biochemical and isotopic responses to salinity stress in quinoa (Chenopodium quinoa Willd.) plants: Prospects for salinity tolerance and yield stability.

Quinoa is an important nutritive crop that can play a strategic role in the development of marginal and degraded lands. Genotypic variations in carbon isotope composition (δ13C), carbon isotope discrimination (Δ13C), ratio of intercellular to atmospheric CO2 concentration (Ci/Ca), intrinsic water use efficiency (iWUE), seed yield and grain protein contents were analyzed in 6 quinoa cultivars grown in the field under saline conditions (0, 10, 20 dS m-1). Significant variations occurred in dry biomass, seed yield, plant height, number of branches, number of panicles, panicle weight, harvest index, N and C content. Some genotypes produced yields with values significantly higher than 2.04 t ha-1 (Q12), with an average increased to 2.58 t ha-1 (AMES22157). The present study indicates a large variation in Δ13C for salinity treatments (3.43‰) and small magnitude of variations among genotypes (0.95‰). Results showed that Δ might be used as an important index for screening, and selection of the salt tolerant quinoa genotypes with high iWUE. Quinoa genotypes differs in foliar 13C and 15N isotope composition, which reflected complex interactions of salinity and plant carbon and nitrogen metabolisms. Grain protein contents were found higher in Q19 and Q31 and lowest in Q26. The study demonstrates that AMES22157 and Q12, were salt tolerant and high yielder while the AMES22157 was more productive. This study provides a reliable measure of morpho-physiological, biochemical and isotopic responses of quinoa cultivars to salinity in hyper arid UAE climate and it may be valuable in the future breeding programs. The development of genotypes having both higher water use efficiency and yield potential would be a very useful contribution for producers in the dry region of Arabian Peninsula.