Ultrastructural description of an unidentified apicomplexan oocyst containing bacteria-like hyperparasites in the gill of Crassostrea rizophorae.

Oocysts of an unidentified coccidian are reported in this study to parasitize the gills of the oyster Crassostrea rizophorae (Mollusca, Bivalvia) collected near the city of Recife (Itamaracá Island, 07 degrees 38' 00" S, 34 degrees 48' 06" W), Brazil. Oocysts appeared as light and dense forms, both containing rod-shaped, bacteria-like hyperparasites (BL). Both light and dense oocysts were spherical, 4.3 to 4.7 pm in diameter, but denser oocysts had irregular contours. Both forms consisted of a thick dense wall (approximately 165 nm thick) consisting of 3 layers. The outermost, a dense and irregular layer about 25 nm thick, possessed numerous bead-like structures and some slender conical projections (up to 1.5 microm long). The inner layer of the wall was formed by a dense and homogenous layer about 125 nm thick. Between these 2 layers, a thin light layer about 12 nm thick was present. Uninucleated sporocysts occupied the internal space of the oocyst and contained some rod-shaped BL and mitochondria surrounded by numerous ribosome-like particles. The dense forms of the oocysts showed the same structures described in the lighter forms and appeared to be the final maturation form of the oocysts. Free sporozoites were occasionally observed among oocysts.

Diagnóstico sanitário em suínos na micro-regiäo homogênea Mata de Ponte Nova, Minas Gerais / Sanitary diagnosis in swynes from homogeneous micro-region Mata de Ponte Nova, Minas Gerais

En este estudio se trató de identificar las principales infecciones que acometen a los suinos, utilizándose 145 animales en la fase de recría y 145 matrices de la micro-región homogénea - Mata de Ponte Nova, Minas Gerais, criados en régimen de confinamiento. Los resultados obtenidos indicaron que 28.6% de los animales, distribuídos en 86.2% de las propiedades estudiadas, poseen oocistos de coccidios (91.5%) de Elimeria 8.4% de Isospora). El porcentaje de rebaños y animales infectados por helmintos (Ascaris, hyostrongylus, oesophagostomum y Trichuris) fue de 89.6% y 30.6%, respectivamente. Eperythrozoon spp fue diagnosticado en 41.2% (12/29) de las criaciones y en 4.1% de los animales (18/435). Los ectoparásitos identificados fueron Hematopinus suis (13.7%) y Sarcoptes scabei var. suis (37.9%). En animales de aquellas propiedades fue investigada la presencia de anticuerpos anti-Brucella sp y anti-Leptospira sp, por medio de pruevas de seroaglutinación rápida y microaglutinación rápida respectivamente, siendo encontrados 3.4% (5/145 matrices) positivos para Brucella sp y 5.2% (23/435 animales) para Leptospira sp

Freedom of coccidial oocysts from Newcastle disease virus.

Oocysts were recovered from chickens experimentally infected with the Mukteswar strain of Newcastle disease virus and Eimeria acervulina or E tenella. Oocysts sterilised by sodium hypochlorite solution were washed, ruptured and inoculated into embryonating eggs which were examined after five days by the haemagglutination test for virus. All these tests were negative. Virus, initially detected with oocysts separated from faeces, was no longer present after sporulation in 2 per cent potassium bichromate. The B1 strain of virus survived for up to 30 hours in 2 per cent sodium hydroxide and 2 per cent potassium bichromate but was quickly destroyed by sodium hypochlorite (specific gravity 1.075).