RESUMO
INTRODUCTION: Factors leading to weight loss and weight stabilization after bariatric surgery are not fully understood. Our aim was to evaluate, in Sprague-Dawley rats, the histological and gut hormonal changes after Larrad-biliopancreatic diversion (Larrad-BPD). MATERIALS AND METHODS: Rats randomly underwent the following protocols: Larrad-BPD (n=4) versus pair fed (PF) (n=4). Weight and food intake were measured every day. By immunohistochemistry ghrelin was examined in the stomach, while cholecystokinin (CCK), glucagon-like-peptide-1 (GLP-1), peptide YY (PYY) and serotonin (5-HT) expression were analyzed in alimentary limb and ileum following or not the Larrad-BPD. RESULTS: Larrad-BPD rats exhibited significant (P<0.05) weight loss compared to PF rats. Villi enlongation was observed in Larrad-BPD rats. In residual stomach, ghrelin was diminished. In the alimentary limb, ghrelin and CCK positive cells were detected more than in the ileum of PF rats. GLP-1 expression was decreased and PYY expression was absent after Larrad-BPD compared with PF rats. DISCUSSION: Larrad-BPD is followed by histological changes and a pleiotropic gut endocrine response aimed to compensate the reduction of intestinal area exposed to food. Until now, the hormones responsible for the intestinal hypertrophy have not been defined.
Assuntos
Desvio Biliopancreático/tendências , Colecistocinina/biossíntese , Mucosa Gástrica/metabolismo , Grelina/biossíntese , Mucosa Intestinal/metabolismo , Animais , Desvio Biliopancreático/métodos , Peso Corporal/fisiologia , Ingestão de Energia/fisiologia , Mucosa Gástrica/citologia , Mucosa Gástrica/fisiopatologia , Mucosa Intestinal/citologia , Mucosa Intestinal/fisiopatologia , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
To investigate cholecystokinin (CCK) mRNA changes induced by social isolation rats were isolated in single cages soon after weaning for 30 days. They were then sacrificed and their brains removed for in situ hybridization (ISH) study. Control animals were housed in groups of 6 per cage for the same period. ISH was performed using a 32P-labelled oligonucleotide probe complementary to CCK-8 mRNA and the results analysed by computerized densitometry. They showed a significant increase (from 59.5-152.3%) in CCK mRNA expression in the basolateral amygdala, cortex, CA1, dorsal raphe nucleus, geniculate body and ventral tegmental area of isolated rats. These results suggest that social isolation may influence CCK gene expression.