RESUMO
Reactive oxygen species (ROS) are important signaling molecules for physiologic processes such as acute response to hypoxia. However, reliable real-time ROS measurement in cells has been a long-standing methodological challenge. Here, we present a protocol to record acute changes in ROS production in sensory cells from mouse carotid body (CB) slices using redox-sensitive green fluorescent protein probes and microfluorimetry. This protocol provides sensitive and reproducible quantification of ROS during acute hypoxia in different subcellular compartments of CB glomus cells. For complete details on the use and execution of this protocol, please refer to Fernández-Agüera et al. (2015) and Arias-Mayenco et al. (2018).
Assuntos
Corpo Carotídeo , Corantes Fluorescentes , Espécies Reativas de Oxigênio , Animais , Corpo Carotídeo/química , Corpo Carotídeo/metabolismo , Feminino , Corantes Fluorescentes/análise , Corantes Fluorescentes/metabolismo , Fluorometria , Histocitoquímica , Masculino , Camundongos , Oxirredução , Espécies Reativas de Oxigênio/análise , Espécies Reativas de Oxigênio/metabolismoRESUMO
We previously reported the immunoreactivity for the vesicular glutamate transporter 2 (VGLUT2) in afferent nerve terminals attached to chemoreceptor type I cells of the carotid body (CB), suggesting that glutamate is released from afferent terminals to stimulate these cells. In the present study, we examined the immunoreactivity for the glutamate-binding subunits of N-methyl-D-aspartate (NMDA) receptors, GluN2A and GluN2B in the rat CB, and the immunohistochemical relationships between these subunits and VGLUT2. Immunoreactivities for GluN2A and GluN2B were predominant in a subpopulation of tyrosine hydroxylase-immunoreactive type I cells rather than those of dopamine beta-hydroxylase-immunoreactive cells. Punctate VGLUT2-immunoreactive products were attached to GluN2A- and GluN2B-immunoreactive type I cells. Bassoon-immunoreactive products were localized between VGLUT2-immunoreactive puncta and type I cells immunoreactive for GluN2A and GluN2B. These results suggest that afferent nerve terminals release glutamate by exocytosis to modulate chemosensory activity of a subpopulation of type I cells via GluN2A- and GluN2B subunits-containing NMDA receptors.
Assuntos
Corpo Carotídeo/metabolismo , Terminações Nervosas/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Proteína Vesicular 2 de Transporte de Glutamato/metabolismo , Animais , Corpo Carotídeo/química , Ácido Glutâmico/metabolismo , Masculino , Terminações Nervosas/química , Ratos , Ratos Wistar , Receptores de N-Metil-D-Aspartato/análiseRESUMO
Many diseases of the respiratory system occur differently in males and females, indicating a possible role of gonadal hormones in respiratory control. We hypothesized that testosterone (T) is important for the ventilatory chemosensitivity responses in males. To test this hypothesis, we evaluated ventilation (VÌ E), metabolic rate and body temperature (Tb) under normoxia/normocapnia, hypercapnia and hypoxia in orchiectomized (ORX), ORX with testosterone replacement (ORX + T) or flutamide (FL, androgen receptor blocker)-treated rats. We also performed immunohistochemistry to evaluate the presence of androgen receptor (AR) in the carotid body (CB) of intact males. Orchiectomy promoted a reduction VÌ E and ventilatory equivalent (VÌ E /VÌ O2) under room-air conditions, which was restored with testosterone treatment. Moreover, during hypoxia or hypercapnia, animals that received testosterone replacement had a higher VÌ E and VÌ E /VÌ O2 than control and ORX, without changes in metabolic and thermal variables. Flutamide decreased the hypoxic ventilatory response without changing the CO2-drive to breathe, suggesting that the testosterone effect on hypercapnic hyperventilation does not appear to involve the AR. We also determined the presence of AR in the CB of intact animals. Our findings demonstrate that testosterone seems to be important for maintaining resting VÌ E in males. In addition, the influence of testosterone on VÌ E, either during resting conditions or under hypoxia and hypercapnia, seems to be a direct and specific effect, as no changes in metabolic rate or Tb were observed during any treatment. Finally, a putative site of testosterone action during hypoxia is the CB, since we detected the presence of AR in this structure.
Assuntos
Regulação da Temperatura Corporal/fisiologia , Hipercapnia/fisiopatologia , Hipóxia/fisiopatologia , Fenômenos Fisiológicos Respiratórios , Testosterona/fisiologia , Antagonistas de Receptores de Andrógenos/farmacocinética , Animais , Corpo Carotídeo/química , Flutamida/farmacologia , Masculino , Orquiectomia , Consumo de Oxigênio/fisiologia , Ratos , Ratos Wistar , Receptores Androgênicos/análise , Receptores Androgênicos/fisiologia , Testosterona/administração & dosagemRESUMO
BACKGROUND: The carotid body (CB) plays a critical role in cyclic intermittent hypoxia (CIH)-induced chemosensitivity; however, the underlying mechanism remains uncertain. We have demonstrated the presence of multiple inotropic glutamate receptors (iGluRs) in CB, and that CIH exposure alters the level of some iGluRs in CB. This result implicates glutamatergic signaling in the CB response to hypoxia. The glutamatergic neurotransmission is not only dependent on glutamate and glutamate receptors, but is also dependent on glutamate transporters, including vesicular glutamate transporters (VGluTs) and excitatory amino acid transporters (EAATs). Here, we have further assessed the expression and distribution of VGluTs and EAATs in human and rat CB and the effect of CIH exposure on glutamate transporters expression. METHODS: The mRNA of VGluTs and EAATs in the human CB were detected by RT-PCR. The protein expression of VGluTs and EAATs in the human and rat CB were detected by Western blot. The distribution of VGluT3, EAAT2 and EAAT3 were observed by immunohistochemistry staining and immunofluorescence staining. Male Sprague-Dawley (SD) rats were exposed to CIH (FIO2 10-21%, 3 min/3 min for 8 h per day) for 2 weeks. The unpaired Student's t-test was performed. RESULTS: Here, we report on the presence of mRNAs for VGluT1-3 and EAAT1-3 in human CB, which is consistent with our previous results in rat CB. The proteins of VGluT1 and 3, EAAT2 and 3, but not VGluT2 and EAAT1, were detected with diverse levels in human and rat CB. Immunostaining showed that VGluT3, the major type of VGluTs in CB, was co-localized with tyrosine hydroxylase (TH) in type I cells. EAAT2 and EAAT3 were distributed not only in type I cells, but also in glial fibrillary acidic protein (GFAP) positive type II cells. Moreover, we found that exposure of SD rats to CIH enhanced the protein level of EAAT3 as well as TH, but attenuated the levels of VGluT3 and EAAT2 in CB. CONCLUSIONS: Our study suggests that glutamate transporters are expressed in the CB, and that glutamate transporters may contribute to glutamatergic signaling-dependent carotid chemoreflex to CIH.
Assuntos
Corpo Carotídeo/metabolismo , Células Quimiorreceptoras/metabolismo , Proteínas de Transporte de Glutamato da Membrana Plasmática/biossíntese , Proteínas Vesiculares de Transporte de Glutamato/biossíntese , Sistema X-AG de Transporte de Aminoácidos/análise , Sistema X-AG de Transporte de Aminoácidos/biossíntese , Sistema X-AG de Transporte de Aminoácidos/genética , Animais , Corpo Carotídeo/química , Células Quimiorreceptoras/química , Expressão Gênica , Proteínas de Transporte de Glutamato da Membrana Plasmática/análise , Proteínas de Transporte de Glutamato da Membrana Plasmática/genética , Humanos , Masculino , Ratos , Ratos Sprague-Dawley , Proteínas Vesiculares de Transporte de Glutamato/análise , Proteínas Vesiculares de Transporte de Glutamato/genéticaRESUMO
The carotid body (CB) is a major peripheral arterial chemoreceptor that initiates respiratory and cardiovascular adjustments to maintain homeostasis. Recent evidence suggests that circulating or locally produced hormones like angiotensin II acting via AT1 receptors modulate its activity in a paracrine-autocrine manner. The aim of this study was to examine the immunohistochemical localization of AT1 receptor in the CB of adult rats and to compare its expression in vehicle-treated animals, and after the long-term application of its selective blocker losartan. Immunohistochemistry revealed that a subset of CB glomeruli and the vast majority of neurons in the adjacent superior cervical ganglion (SCG) were strongly AT1 receptor-immunoreactive. In the CB immunostaining was observed in the chemosensory glomus cells typically aggregated in cell clusters while the nerve fibers in-between and large capillaries around them were immunonegative. Exogenous administration of losartan for a prolonged time significantly reduces the intensity of AT1 receptor immunostaining in the CB glomus cells and SCG neurons. Our results show that AT1 receptors are largely expressed in the rat CB under physiological conditions, and their expression is down-regulated by losartan treatment.
Assuntos
Corpo Carotídeo/química , Receptor Tipo 1 de Angiotensina/química , Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Animais , Imuno-Histoquímica , Losartan/farmacologia , Masculino , Ratos , Receptor Tipo 1 de Angiotensina/metabolismoRESUMO
The carotid body, located at the bifurcation of the common carotid artery, is a small sensory organ that detects changes in oxygen concentration and plays a vital role in controlling respiration. Although several molecules, such as neurotransmitters and neuropeptides, are involved in the regulation of the respiratory system, their detailed mechanisms have not been established yet. This study identifies that the presence of manserin, a neuropeptide, in the carotid body may play a crucial role in regulating respiration. The carotid bodies of adult Wistar rats were perfused with paraformaldehyde, and the frozen sections were subjected to immunohistochemical analyses. The carotid body comprises two distinct types of cells, neuron-like glomus cells and glial-like sustentacular cells. We used specific antibodies to distinguish the specific location of manserin in the carotid body, which included a tyrosine hydroxylase-positive antibody for glomus cells and an S100 protein antibody for sustentacular cells. Immunofluorescence analysis revealed that while tiny, round signals were exclusively observed in the cytoplasm of glomus cells, no signals were observed in sustentacular cells. Because manserin is believed to be secreted from precursor proteins by the endoproteolytic processing of a large precursor protein called secretogranin II, manserin secretion systems may exist in the carotid body, and thus, behave as potential regulators of respiration in the carotid body.
Assuntos
Corpo Carotídeo/química , Neuropeptídeos/química , Fragmentos de Peptídeos/química , Animais , Imuno-Histoquímica , Ratos , Ratos WistarRESUMO
The carotid body (CB) is the main peripheral chemoreceptor that senses the arterial PO2, PCO2 and pH. In response to hypoxemia, hypercapnia and acidosis, carotid chemosensory discharge elicits reflex respiratory, autonomic and cardiovascular adjustments. The classical construct considers the CB as the main peripheral oxygen sensor, triggering reflex physiological responses to acute hypoxemia and facilitating the ventilatory acclimation to chronic hypoxemia at high altitude. However, a growing body of experimental evidence supports the novel concept that an abnormally enhanced CB chemosensory input to the brainstem contributes to overactivation of the sympathetic nervous system, and consequent pathology. Indeed, the CB has been implicated in several diseases associated with increases in central sympathetic outflow. These include hypertension, heart failure, sleep apnea, chronic obstructive pulmonary disease and metabolic syndrome. Indeed, ablation of the CB has been proposed for the treatment of severe and resistant hypertension in humans. In this review, we will analyze and discuss new evidence supporting an important role for the CB chemoreceptor in the progression of autonomic and cardiorespiratory alterations induced by heart failure, obstructive sleep apnea, chronic obstructive pulmonary disease and metabolic syndrome.
Assuntos
Corpo Carotídeo/fisiopatologia , Insuficiência Cardíaca/fisiopatologia , Doenças Metabólicas/fisiopatologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Apneia Obstrutiva do Sono/fisiopatologia , Sistema Nervoso Simpático/fisiopatologia , Corpo Carotídeo/química , Insuficiência Cardíaca/etiologia , Humanos , Doenças Metabólicas/etiologia , Doença Pulmonar Obstrutiva Crônica/etiologia , Fatores de Risco , Apneia Obstrutiva do Sono/etiologiaRESUMO
The carotid body is a highly specialized chemoreceptive organ of neural crest origin whose role is to detect changes in arterial oxygen content. The sensory units are the chemoreceptor cells, which are neuronal-like cells, surrounded by sustentacular or glial-like cells. It is suggested that the carotid body contains self-renewing multipotent stem cells, which are putatively represented by glial-like sustentacular cells. The mechanisms of renewal of neuronal-like cells are unclear. Recently, we have demonstrated the expression of galanin, a peptide promoting neurogenesis, in chemoreceptor cells in the human CB. Thus, in the present study we seek to determine whether galanin expression in chemoreceptor cells could be matched with that of nestin, a peptide that is a marker of multipotent neural stem cells, or rather with the glial fibrillary acidic protein (GFAP), a marker for glial cells. The latter would underscore the pluasibly essential role of sustentacular cells in the self-renewal capability of chemorecetors. We found that galanin expression is matched with nestin in chemoreceptor cells of the human carotid body, but not with that of GFAP. Thus, galanin expression in chemoreceptor cells could provide a signal for neurogenesis and chemoreceptor cell differentiation in the carotid body.
Assuntos
Corpo Carotídeo/química , Galanina/análise , Nestina/análise , Adulto , Idoso , Proteína Glial Fibrilar Ácida/análise , Humanos , Imuno-Histoquímica , Pessoa de Meia-IdadeRESUMO
The carotid body (CB) is the main peripheral chemoreceptor that senses the arterial PO2, PCO2 and pH. In response to hypoxemia, hypercapnia and acidosis, carotid chemosensory discharge elicits reflex respiratory, autonomic and cardiovascular adjustments. The classical construct considers the CB as the main peripheral oxygen sensor, triggering reflex physiological responses to acute hypoxemia and facilitating the ventilatory acclimation to chronic hypoxemia at high altitude. However, a growing body of experimental evidence supports the novel concept that an abnormally enhanced CB chemosensory input to the brainstem contributes to overactivation of the sympathetic nervous system, and consequent pathology. Indeed, the CB has been implicated in several diseases associated with increases in central sympathetic outflow. These include hypertension, heart failure, sleep apnea, chronic obstructive pulmonary disease and metabolic syndrome. Indeed, ablation of the CB has been proposed for the treatment of severe and resistant hypertension in humans. In this review, we will analyze and discuss new evidence supporting an important role for the CB chemoreceptor in the progression of autonomic and cardiorespiratory alterations induced by heart failure, obstructive sleep apnea, chronic obstructive pulmonary disease and metabolic syndrome.
Assuntos
Humanos , Sistema Nervoso Simpático/fisiopatologia , Corpo Carotídeo/fisiopatologia , Apneia Obstrutiva do Sono/fisiopatologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Insuficiência Cardíaca/fisiopatologia , Doenças Metabólicas/fisiopatologia , Corpo Carotídeo/química , Fatores de Risco , Apneia Obstrutiva do Sono/etiologia , Doença Pulmonar Obstrutiva Crônica/etiologia , Insuficiência Cardíaca/etiologia , Doenças Metabólicas/etiologiaRESUMO
The carotid body (CB) is a polymodal chemoreceptor that triggers the hyperventilatory response to hypoxia necessary for the maintenance of O(2) homeostasis essential for the survival of organs such as the brain or heart. Glomus cells, the sensory elements in the CB, are also sensitive to hypercapnia, acidosis and, although less generally accepted, hypoglycemia. Current knowledge on CB function is mainly based on studies performed on lower mammals, but the information on the human CB is scant. Here we describe the structure, neurotrophic properties, and cellular responses to hypoxia and hypoglycemia of CBs dissected from human cadavers. The adult CB parenchyma contains clusters of chemosensitive glomus (type I) and sustentacular (type II) cells as well as nestin-positive progenitor cells. This organ also expresses high levels of the dopaminotrophic glial cell line-derived neurotrophic factor (GDNF). GDNF production and the number of progenitor and glomus cells were preserved in the CBs of human subjects of advanced age. As reported for other mammalian species, glomus cells responded to hypoxia by external Ca(2+)-dependent increase of cytosolic [Ca(2+)] and quantal catecholamine release. Human glomus cells are also responsive to hypoglycemia and together the two stimuli, hypoxia and hypoglycemia, can potentiate each other's effects. The chemo-sensory responses of glomus cells are also preserved at an advanced age. Interestingly, a neurogenic niche similar to that recently described in rodents is also preserved in the adult human CB. These new data on the cellular and molecular physiology of the CB pave the way for future pathophysiological studies involving this organ in humans.
Assuntos
Corpo Carotídeo/fisiologia , Cálcio/metabolismo , Corpo Carotídeo/química , Corpo Carotídeo/citologia , Fator Neurotrófico Derivado de Linhagem de Célula Glial/análise , Humanos , Hipoglicemia/fisiopatologia , Hipóxia/fisiopatologiaRESUMO
The carotid body is a neural-crest-derived organ devoted to respiratory homeostasis through sensing changes in blood oxygen levels. The sensory units are the glomeruli composed of clusters of neuronal-like (type I) cells surrounded by glial-like (type II) cells. During chronic hypoxia, the carotid body shows growth, with increasing neuronal-like cell numbers. We are interested in the signals involved in the mechanisms that underlie such response, because they are not well understood and described. Considering that, in literature, galanin is involved in neurotrophic or neuroprotective role in cell proliferation and is expressed in animal carotid body, we investigated its expression in human. Here, we have shown the expression and localisation of galanin in the human carotid body.
Assuntos
Corpo Carotídeo/química , Galanina/análise , Neurônios/química , Adulto , Idoso , Corpo Carotídeo/citologia , Corpo Carotídeo/fisiologia , Galanina/fisiologia , Humanos , Pessoa de Meia-IdadeRESUMO
Functional maturation of the carotid body in the postnatal period relies partly on structural and neurochemical changes, which are reviewed here. Structural changes include changes in cytological composition, and increases in glomic tissue volume, dense-cored granules of type I cells, synapses of type I cells with type II cells and afferent nerve fibres. Vascular volume also increases, but in the same proportion as extravascular volume. During maturation, the carotid body also shows higher density and hypoxic sensitivity of K(+)-channels and an increased hypoxic [Ca(2+)](i) response. Modulation of content and release of catecholamine occurs, together with decreased expression of tyrosine hydroxylase and dopamine ß-hydroxylase and increased expression of choline acetyltransferase. Expression of dopamine 2 receptor and nicotinic α3 and α7 receptor subunits increases, and muscarinic M1 receptor protein, nicotinic α4 and ß2 receptor subunits and adenosine receptor 1 decrease. Maturation of the carotid body may also be explained with reference to the developmentally regulated expression of trophic factors and their receptors.
Assuntos
Corpo Carotídeo/química , Corpo Carotídeo/crescimento & desenvolvimento , Corpo Carotídeo/metabolismo , Animais , HumanosRESUMO
Although connexin36 (Cx36) has been studied in several tissues, it is notable that no data are available on Cx36 expression in the carotid body and the intestine. The present study was undertaken to evaluate using immunohistochemistry, PCR and Western blotting procedures, whether Cx36 was expressed in the mouse carotid body and in the intestine at ileum and colon level. In the carotid body, Cx36 was detected as diffuse punctate immunostaining and as protein by Western blotting and mRNA by RT-PCR. Cx36 punctate immunostaining was also evident in the intestine with localization restricted to the myenteric plexus of both the ileum and the colon, and this detection was also confirmed by Western blotting and RT-PCR. All the data obtained were validated using Cx36 knockout mice. Taken together the present data on localization of Cx36 gap-junctions in two tissues of neural crest-derived neuroendocrine organs may provide an anatomical basis for future functional investigations.
Assuntos
Corpo Carotídeo/metabolismo , Conexinas/análise , Conexinas/genética , Plexo Mientérico/metabolismo , Animais , Western Blotting , Corpo Carotídeo/química , Conexinas/deficiência , Conexinas/metabolismo , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Plexo Mientérico/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteína delta-2 de Junções ComunicantesRESUMO
The carotid body (CB) is the main arterial chemoreceptor with a low threshold to hypoxia. CB activity is augmented by A(2)-adenosine receptors stimulation and attenuated by D(2)-dopamine receptors. The effect of aging on ventilatory responses mediated by the CB to hypoxia, ischemia, and to adenosine and dopamine administration is almost unknown. This study aims to investigate the ventilatory response to ischemia and to adenosine, dopamine, and their antagonists in old rats, as well as the effect of hypoxia on adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in the aged CB. In vivo experiments were performed on young and aged rats anesthetized with pentobarbitone and breathing spontaneously. CB ischemia was induced by bilateral common carotid occlusions. cAMP content was measured in CB incubated with different oxygen concentrations. Hyperoxia caused a decrease in cAMP in the CB at all ages, but no differences were found between normoxia and hypoxia or between young and old animals. The endogenous dopaminergic inhibitory tonus is slightly reduced. However, both the ventilation decrease caused by exogenous dopamine and the increase mediated by A(2A)-adenosine receptors are not impaired in aged animals. The bradycardia induced by adenosine is attenuated in old rats. The CB's peripheral control of ventilation is preserved during aging. Concerns have also arisen regarding the clinical usage of adenosine to revert supraventricular tachycardia and the use of dopamine in critical care situations involving elderly people.
Assuntos
Adenosina/farmacologia , Envelhecimento/fisiologia , Corpo Carotídeo/fisiologia , Dopamina/farmacologia , Hipóxia/fisiopatologia , Isquemia/fisiopatologia , Antagonistas do Receptor A2 de Adenosina/farmacologia , Animais , Corpo Carotídeo/irrigação sanguínea , Corpo Carotídeo/química , Corpo Carotídeo/efeitos dos fármacos , AMP Cíclico/fisiologia , Modelos Animais de Doenças , Antagonistas de Dopamina/farmacologia , Masculino , Ventilação Pulmonar/efeitos dos fármacos , Ventilação Pulmonar/fisiologia , Ratos , Ratos Wistar , Receptor A2A de Adenosina/metabolismoRESUMO
The physiological application of amperometric adenosine triphosphate (ATP) microbiosensors for characterizing the stimulus-response at rat carotid bodies superfused with high potassium concentrations, during normoxic hypercapnia, and during hypoxia is demonstrated using the peripheral arterial chemoreceptors in the carotid body of rats as a model system. Amperometric microbiosensors based on glucose oxidase (GOD) and hexokinase (HEX) immobilized within a polymer matrix at the surface of Pt disk microelectrodes (diameter: 25 microm) are positioned at a distance of approximately 100 microm above the carotid body surface for detecting extracellular ATP. A linear calibration function of ATP microbiosensors in the physiologically relevant concentration range of 0-40 microM ATP enables quantitative detection of ATP released at the carotid body surface in response to physiological stimuli. It is shown that these stimuli induce extracellular ATP release from the carotid body at levels of 4-10 microM. Other electroactive neurotransmitters such as, e.g., catecholamines are coreleased by the carotid body at hypercapnic, hypoxic and high-potassium stimulus, are simultaneously detected utilizing a dual-electrode assembly with an ATP microbiosensor and a second bare channel providing a colocalized reference measurement for ATP quantification.
Assuntos
Trifosfato de Adenosina/metabolismo , Técnicas Biossensoriais/instrumentação , Corpo Carotídeo/química , Corpo Carotídeo/metabolismo , Animais , Calibragem , Catecolaminas/metabolismo , Condutividade Elétrica , Eletrodos , Hipercapnia/metabolismo , Hipóxia/metabolismo , Potássio/farmacologia , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Carotid body (CB) is a round to ovoid or flattened structure situated within the adventitia of the common carotid artery bifurcation on both sides of the neck. CB contains two basic types of cells: chief cells (or glomus type 1) and sustentacular cells (glomus type 2). Carotid body tumor (CBT) or paraganglioma arises from the chief cells of the carotid body. The diagnosis of CBT is typically made with radiological studies. Fine needle aspiration biopsy (FNAB) is seldom requested for this purpose due to rare but dreadful reported complications such as hemorrhage and damage to the carotid artery. In this report we discuss the cytological findings of a malignant CBT diagnosed by FNAB in a 22 year-old female.
Assuntos
Biópsia por Agulha Fina , Tumor do Corpo Carotídeo/diagnóstico , Corpo Carotídeo/patologia , Neoplasias Primárias Múltiplas/patologia , Adulto , Biomarcadores Tumorais/análise , Corpo Carotídeo/química , Tumor do Corpo Carotídeo/química , Tumor do Corpo Carotídeo/secundário , Tumor do Corpo Carotídeo/cirurgia , Cromograninas/análise , Feminino , Humanos , Linfonodos/patologia , Sinaptofisina/análiseRESUMO
Hypoxic stimulation of the carotid body receptors (CBR) results in a rapid hyperglycemia with an increase in brain glucose retention. Previous work indicates that neurohypophysectomy inhibits this hyperglycemic response. Here, we show that systemic arginine vasopressin (AVP) induced a transient, but significant, increase in blood glucose levels and increased brain glucose retention, a response similar to that observed after CBR stimulation. Comparable results were obtained after intracerebral infusion of AVP. Systemic AVP-induced changes were maintained in hypophysectomized rats but were not observed after adrenalectomy. Glycemic changes after CBR stimulation were inhibited by pharmacological blockage of AVP V1a receptors with a V1a-selective receptor antagonist ([beta-Mercapto-beta,beta-cyclopentamethylenepropionyl1,O-me-Tyr2, Arg8]-vasopressin). Importantly, local application of micro-doses of this antagonist to the liver was sufficient to abolish the hyperglycemic response after CBR stimulation. These results suggest that AVP is a mediator of the hyperglycemic reflex and cerebral glucose retention following CBR stimulation. We propose that hepatic activation of AVP V1a receptors is essential for this hyperglycemic response.
Assuntos
Arginina Vasopressina/fisiologia , Corpo Carotídeo/fisiologia , Células Quimiorreceptoras/efeitos dos fármacos , Células Quimiorreceptoras/fisiologia , Inibidores Enzimáticos/farmacologia , Glucose/metabolismo , Cianeto de Sódio/farmacologia , Glândulas Suprarrenais/fisiologia , Adrenalectomia , Animais , Antagonistas dos Receptores de Hormônios Antidiuréticos , Química Encefálica , Corpo Carotídeo/química , Catecolaminas/metabolismo , Glucagon/metabolismo , Hiperglicemia/induzido quimicamente , Hiperglicemia/fisiopatologia , Hipóxia/fisiopatologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Ratos , Ratos Wistar , Receptores de Vasopressinas/fisiologia , Vasopressinas/farmacologiaRESUMO
We studied by immunocytochemistry the expression of AM in human carotid bodies, sampled at autopsy from 16 adult subjects (mean age+/-S.D.: 44.3+/-3.4 years) and from six fetuses (mean gestational age+/-S.D.: 167+/-11 days). No AM immunoreactivity was visible in the type II cells of both series. The percentage of immunoreactive type I cells was higher in the adult subjects (32.3+/-7.7%) with respect to the fetuses (11.8+/-2.7%, P < 0.001). Dark cells showed a higher percentage of positive immunoreaction with respect to light cells, both in adult subjects (61.7+/-13.4% versus 19.2+/-5.2%) and in fetuses (25.3+/-4.4% versus 6.2+/-2.0%). AM may play a role in the regulation of chemoreceptor discharge through paracrine releasing action and/or vasodilator effect. The low expression of AM in fetuses may be ascribed to the absence of pulmonary respiration with lack of regulatory role of the carotid body during the prenatal period.
Assuntos
Adrenomedulina/imunologia , Adrenomedulina/metabolismo , Corpo Carotídeo/imunologia , Corpo Carotídeo/metabolismo , Adrenomedulina/biossíntese , Adulto , Apoptose/fisiologia , Corpo Carotídeo/química , Corpo Carotídeo/citologia , Hipóxia Celular/fisiologia , Feminino , Feto/imunologia , Imunofluorescência , Humanos , Imuno-Histoquímica , Masculino , Comunicação Parácrina/fisiologia , Receptores de Superfície Celular/metabolismo , Vasodilatação/fisiologiaRESUMO
Comparar Doppler Triplex y Scion Image® para obtener un análisis computarizado de la ecogenicidadque caracteriza cualitativamente a los tejidos estudiados en las imágenes médicas adquiridas. Se examinó la región cervical bilateralmente a una muestra de individuos adultos jóvenes de ambos sexos. Se procedió a realizar un histograma computarizado en una región de interés seleccionada al azar de la arteria carótida común y del músculo esternocleidomastoideo, analizando los datos obtenidos con el método estadístico HOMALS. Se observó que la mediana para los valores altos y bajos en la escala de gris de la arteria carótida común y del músculo esternocleidomastoideo, tiene similitud con la de los valores altos y bajos de la velocidad pico sistólica de la arteria carótida común. Asimismo, la mediana de las variables categóricas correspondientes a la escala de gris de la arteria carótida común y del músculo esternocleidomastoideo, también tiene similitud con la de los valores de la velocidad al final de la diástole de la arteria carótida común. La comparación de Doppler Triplex con Scion Image® es factible, resultando estadísticamente discriminante para el estudio del sistema carotídeo extracraneal.
To compare Doppler Triplex and Scion Image® to obtain a computed analysis of the echogenicity that qualitatively characterizes to the tissues studied in the acquired medical images. It was examined the cervical region bilaterally to a sample of young mature individuals of both sexes. It was proceeded to carry out an computed histogram in a region of interest selected at random of the common carotid artery and the sternomastoid muscle, analyzing the obtained data with the statistical method HOMALS. It was observed that the median for the upper and under values in the grayscale of the common carotid artery and of the sternomastoid muscle, this has similarity with that of the upper and under values of the peak systolic velocity of the common carotid artery. Also, it could be appreciated that the median of the values of the categorical variables corresponding to the grayscale of the common carotid artery and of the sternomastoid muscle, this also has similarity with that of the peak end diastolic velocity of the common carotid artery. The comparison of Doppler Triplex with Scion Image® is feasible, being statistically discriminant for the study of the extracranial carotid system.