RESUMO
BACKGROUND: The detection rate of human papillomavirus (HPV) in Bowen's disease (BD) varies greatly. OBJECTIVES: To detect HPV DNA in BD samples using next-generation sequencing (NGS) and compare HPV detection rates between pelvic and non-pelvic BD. MATERIALS AND METHODS: We evaluated 99 patients with BD in our institution. DNA was extracted from formalin-fixed and paraffin embedded (FFPE) tissue blocks. The presence of HPV DNA material was detected using special kit-based NGS technology. Clinical characteristics and HPV detection rates were then compared between pelvic and non-pelvic BD samples. RESULTS: HPV was detected in 26 (26.3%) BD samples. A total of 10 types of α-HPV was detected: HPV 16, 53, 31, 58, 66, 26, 27, 57, 45, and 72. The most common HPV type was 16 (12.1%). Only two types (27 and 57) were frequently classified as cutaneous type, and the rest were mucosal types. The HPV detection rate was significantly higher in pelvic BD (45.2%) compared to non-pelvic BD (17.6%). CONCLUSION: The present study suggests that sexually transmitted mucosal α-HPV plays a significant role in the pathogenesis of BD, especially in the pelvic region.
Assuntos
Alphapapillomavirus/isolamento & purificação , Doença de Bowen/virologia , DNA Viral/isolamento & purificação , Infecções por Papillomavirus/virologia , Doenças Virais Sexualmente Transmissíveis/virologia , Pele/virologia , Parede Abdominal/patologia , Parede Abdominal/virologia , Idoso , Idoso de 80 Anos ou mais , Alphapapillomavirus/genética , Biópsia , Doença de Bowen/etiologia , Nádegas , DNA Viral/genética , Feminino , Genótipo , Técnicas de Genotipagem , Virilha/patologia , Virilha/virologia , Sequenciamento de Nucleotídeos em Larga Escala , Papillomavirus Humano 16/genética , Papillomavirus Humano 16/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa/patologia , Mucosa/virologia , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/genética , Estudos Retrospectivos , Doenças Virais Sexualmente Transmissíveis/complicações , Pele/patologia , Coxa da Perna/patologia , Coxa da Perna/virologiaAssuntos
Infecções por Citomegalovirus/virologia , Febre/complicações , Infecções por HIV/complicações , Linfoma Relacionado a AIDS/tratamento farmacológico , Neutropenia/complicações , Protocolos de Quimioterapia Combinada Antineoplásica , Antivirais/uso terapêutico , Ciclofosfamida , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/tratamento farmacológico , Doxorrubicina , Febre/sangue , Febre/diagnóstico , Foscarnet/uso terapêutico , Infecções por HIV/sangue , Humanos , Imuno-Histoquímica , Linfoma Relacionado a AIDS/sangue , Linfoma Relacionado a AIDS/complicações , Linfoma Relacionado a AIDS/virologia , Masculino , Pessoa de Meia-Idade , Necrose/patologia , Necrose/virologia , Neutropenia/diagnóstico , Prednisona , Pele/patologia , Pele/virologia , Coxa da Perna/patologia , Coxa da Perna/virologia , VincristinaRESUMO
Latency within the nervous system is a characteristic feature of herpesviridae infection. It is reactivated by triggering factors such as UV exposure, stress, and trauma. Simultaneous reactivation of herpes simplex and herpes zoster is uncommon, however, an observation provably explained by differences in the triggering mechanism. Concurrent reactivation of herpes simplex virus (HSV) and varicella zoster virus (VZV) is occasionally encountered in immunosuppressed patients; on the other hand, it is rarely reported in immunocompetent individuals. We present the case of an immunocompetent 8-yr-old female patient with concurrent reactivation of HSV on the face and VZV on the right L2 dermatome.
Assuntos
Herpes Simples/virologia , Herpes Zoster/virologia , Herpesvirus Humano 3/fisiologia , Simplexvirus/fisiologia , Ativação Viral , Nádegas/patologia , Nádegas/virologia , Criança , Face , Feminino , Herpes Simples/complicações , Herpes Simples/diagnóstico , Herpes Simples/patologia , Herpes Zoster/complicações , Herpes Zoster/diagnóstico , Herpes Zoster/patologia , Humanos , Imunocompetência , Coxa da Perna/patologia , Coxa da Perna/virologiaRESUMO
We report a case of a 24-year-old female patient with a history of giant and multiple lesions of condylomata acuminata on the vulva and breasts which were treated by surgical excision.