Assuntos
Receptores Acoplados a Proteínas G/química , Receptores Acoplados a Proteínas G/metabolismo , Comportamento Competitivo , Cristalização/história , Cristalografia por Raios X/história , História do Século XX , História do Século XXI , Humanos , Prêmio Nobel , Receptores Adrenérgicos beta 2/química , Receptores Adrenérgicos beta 2/história , Receptores Acoplados a Proteínas G/história , Espectrometria de FluorescênciaRESUMO
While crystallization historically predates crystallography, it is a critical step for the crystallographic process. The rich history of crystallization and how that history influences current practices is described. The tremendous impact of crystallization screens on the field is discussed.
Assuntos
Cristalização/história , Cristalografia por Raios X/história , Proteínas/química , Animais , Bactérias/química , Cristalização/instrumentação , Cristalização/métodos , Cristalografia por Raios X/instrumentação , Cristalografia por Raios X/métodos , Difusão , História do Século XX , História do Século XXI , Humanos , Transição de Fase , VolatilizaçãoAssuntos
Cristalização/métodos , Cristalografia por Raios X/métodos , Proteínas de Membrana/química , Bacteriorodopsinas/química , Cristalização/história , Cristalização/tendências , Cristalografia por Raios X/história , Cristalografia por Raios X/tendências , História do Século XX , História do Século XXI , Lipídeos/químicaRESUMO
The saga of liquid crystals started with their discovery in 1888 by the botanist Friedrich Reinitzer, who unexpectedly observed "two melting points" for crystals extracted from the root of a carrot. At the end of the nineteenth century, most scientists did not believe in the existence of "liquid crystals" as promoted by the crystallographer Otto Lehmann. The controversies were very vivid; to the point that the recognition of mesomorphic states of matter by the scientific community required more than two decades. In the end, liquid crystals have changed our vision of matter by shattering the three-state paradigm. Since the mid-1970s, liquid crystals have revolutionized the worldwide information-display industry and now play a host of key roles in various technologies.
Assuntos
Cristais Líquidos/história , Coloides/análise , Coloides/história , Cristalização/história , Daucus carota/química , Daucus carota/história , Desenho de Equipamento , Alemanha , História do Século XIX , História do Século XX , Cristais Líquidos/análise , Microscopia/história , Microscopia/instrumentação , Raízes de Plantas/químicaRESUMO
Protein crystallization has been known since 1840 and can prove to be straightforward but, in most cases, it constitutes a real bottleneck. This stimulated the birth of the biocrystallogenesis field with both 'practical' and 'basic' science aims. In the early years of biochemistry, crystallization was a tool for the preparation of biological substances. Today, biocrystallogenesis aims to provide efficient methods for crystal fabrication and a means to optimize crystal quality for X-ray crystallography. The historical development of crystallization methods for structural biology occurred first in conjunction with that of biochemical and genetic methods for macromolecule production, then with the development of structure determination methodologies and, recently, with routine access to synchrotron X-ray sources. Previously, the identification of conditions that sustain crystal growth occurred mostly empirically but, in recent decades, this has moved progressively towards more rationality as a result of a deeper understanding of the physical chemistry of protein crystal growth and the use of idea-driven screening and high-throughput procedures. Protein and nucleic acid engineering procedures to facilitate crystallization, as well as crystallization methods in gelled-media or by counter-diffusion, represent recent important achievements, although the underlying concepts are old. The new nanotechnologies have brought a significant improvement in the practice of protein crystallization. Today, the increasing number of crystal structures deposited in the Protein Data Bank could mean that crystallization is no longer a bottleneck. This is not the case, however, because structural biology projects always become more challenging and thereby require adapted methods to enable the growth of the appropriate crystals, notably macromolecular assemblages.
Assuntos
Cristalização/história , Proteínas/química , Animais , Cristalografia por Raios X , História do Século XIX , História do Século XX , História do Século XXI , HumanosAssuntos
Química , Cristalografia por Raios X , Prêmio Nobel , Ribossomos/química , Antibacterianos/farmacologia , Química/história , Cristalização/história , Cristalografia por Raios X/história , História do Século XX , História do Século XXI , Humanos , Ribossomos/efeitos dos fármacos , Ribossomos/metabolismoRESUMO
In 1946 three Americans shared the Nobel Prize for Chemistry--James Sumner 'for his discovery that enzymes can be crystallized', and John Northrop and Wendell Stanley 'for their preparation of enzymes and virus proteins in a pure form'. The award made history as the first time that the Chemistry Prize was shared by three individuals. It also doubled the number of Americans that had received the Chemistry award since its inception in 1901.