RESUMO
A simple Na,K-ATPase assay is described as a suitable method for testing of digoxin photodegradation. The exposure of Na,K-ATPase to the photodegraded samples exhibited reduced inhibition of the enzyme, compared to the unirradiated samples containing equal initial concentrations of drug. The degree of inhibition was dependent on the irradiation time. The concentrations of digoxin in irradiated samples were evaluated by HPLC analysis. Excellent agreement of the results obtained by both methods was observed. The investigation of the influence of irradiated samples on Na,K-ATPase inhibition revealed no side products acting as Na,K-ATPase inhibitors. The cytokinesis block micronucleus test (CBMN) was applied in order to investigate the cytotoxicity of the possible degradation products after exposure to UV irradiation. The results confirmed that the photochemical treatment did not induce the cytotoxic side products. Zero order kinetics, which was observed for digoxin photodegradation and the associated reaction mechanism are also discussed.
Assuntos
Cardiotônicos/análise , Digoxina/análise , ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores , Cardiotônicos/farmacologia , Cardiotônicos/efeitos da radiação , Cromatografia Líquida de Alta Pressão , Digoxina/farmacologia , Digoxina/efeitos da radiação , Relação Dose-Resposta a Droga , Estabilidade de Medicamentos , Humanos , Cinética , Luz , Linfócitos/efeitos dos fármacos , Testes para Micronúcleos , FotoquímicaRESUMO
Ouabain, alpha-acetyldigoxin and digoxin were subjected to irradiation using different light sources in crystalline state and their respective yields of photoproducts were determined densitometrically. alpha-Acetyldigoxin was found to be less stable than digoxin yielding a higher percentage of photoproducts under each light source examined. Ouabain showed photostability under the conditions of investigation.