Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Doença de Hodgkin , Schistosoma mansoni , Esquistossomose mansoni , Animais , Criança , Ciclofosfamida/administração & dosagem , Dacarbazina/administração & dosagem , Doença de Hodgkin/diagnóstico , Doença de Hodgkin/tratamento farmacológico , Doença de Hodgkin/parasitologia , Doença de Hodgkin/patologia , Humanos , Masculino , Prednisolona/administração & dosagem , Esquistossomose mansoni/diagnóstico , Esquistossomose mansoni/tratamento farmacológico , Esquistossomose mansoni/patologia , Vincristina/administração & dosagemAssuntos
Doenças da Medula Óssea/parasitologia , Filariose/parasitologia , Doença de Hodgkin/parasitologia , Microfilárias/crescimento & desenvolvimento , Wuchereria bancrofti/crescimento & desenvolvimento , Adulto , Animais , Humanos , Masculino , Microfilárias/isolamento & purificação , Pescoço , Wuchereria bancrofti/isolamento & purificaçãoRESUMO
The case of an HIV-positive man treated for acute toxoplasmosis with no traces of malignancy is reported. A second lymph node extirpation was performed after 5 months, which identified the presence of Hodgkin and Reed-Sternberg (HRS) cells. This case suggests that toxoplasmosis may cause changes in the regulation of surrounding cells and induce neoplastic proliferation.
Assuntos
Doença de Hodgkin/parasitologia , Toxoplasmose/complicações , Adulto , Infecções por HIV/complicações , Infecções por HIV/parasitologia , Infecções por HIV/patologia , Doença de Hodgkin/patologia , Doença de Hodgkin/virologia , Humanos , Masculino , Células de Reed-Sternberg/citologia , Toxoplasmose/patologia , Toxoplasmose/virologiaAssuntos
Doença de Hodgkin/parasitologia , Strongyloides stercoralis , Estrongiloidíase , Adulto , Animais , Humanos , MasculinoRESUMO
Using both traditional methods and broad-range 18S ribosomal DNA (rDNA) polymerase chain reaction, we examined 2 cases of lethal cestodiasis, in which the disease agent had been poorly identified or misidentified. In one case, involving a patient with AIDS, we identified the human dwarf tapeworm, Hymenolepis nana, as a cause of aberrant metastatic larval disease. In the second case with similar pathologic abnormalities, involving a patient with Hodgkin disease, we identified a larval cestode with a previously uncharacterized 18S rDNA sequence. A prior report of this case nearly 30 years ago, based on tissue examination, had suggested that the parasite was a sparganum.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Síndrome da Imunodeficiência Adquirida/imunologia , Infecções por Cestoides/complicações , Infecções por Cestoides/imunologia , Doença de Hodgkin/complicações , Doença de Hodgkin/imunologia , Síndrome da Imunodeficiência Adquirida/parasitologia , Animais , Cestoides/genética , Cestoides/isolamento & purificação , DNA Ribossômico/análise , Evolução Fatal , Doença de Hodgkin/parasitologia , Humanos , Masculino , Infecções Oportunistas/complicações , Infecções Oportunistas/imunologia , Infecções Oportunistas/parasitologia , Filogenia , RNA Ribossômico 18S/análiseRESUMO
In order to investigate the morbidity of toxoplasmic lymphadenitis, polymerase chain reaction (PCR) was used to detect DNA of Toxoplasma gondii within lymph nodes in 3 groups of 120 patients with different diseases. After extracting the DNA of each sample, PCR was employed to amplify toxoplasma DNA. The results showed that the amplification product of 210 bp was confirmed in 7 patients: 3 cases of Hodgkin's disease (HD), 2 cases of non-Hodgkin's lymphoma (NHL) and 2 cases of chronic lymphadenitis (CL). Each PCR product was then subjected to Southern blot hybridization. Besides the 7 cases proved by PCR, 1 case of CL was found positive. The positive percentages of HD, NHL and CL were 9.38% (3/32), 4.88% (2/41), and 6.38% (3/47), respectively. The total positive rate was 6.67% (8/120).
Assuntos
DNA de Protozoário/isolamento & purificação , Linfonodos/parasitologia , Linfadenite/parasitologia , Toxoplasma/isolamento & purificação , Animais , Doença Crônica , Feminino , Doença de Hodgkin/parasitologia , Humanos , Linfoma não Hodgkin/parasitologia , Masculino , Reação em Cadeia da Polimerase , Toxoplasmose/parasitologiaRESUMO
AIM: To detect the presence of Toxoplasma gondii in lymph node tissue in patients with Toxoplasma infection. METHODS: T. gondii (RH strain) specific DNA fragment clones were obtained by using PCR and gene recombination technique. The DNA fragments used as hybridization probes were labelled with digoxigenin by random primer method. The technique of in situ hybridization (ISH) was used to detect T. g DNA in the lymph node sections. RESULTS: Four out of 120 samples T. g DNA were found positive, one with Hodgkin's disease (HD) (1/32), one with non-Hodgkin's lymphoma (NHL) (1/41) and 2 with chronic lymphadenitis (CL) (2/47). The total positive rate was 3.3%. It was demonstrated that this highly specific probe could detect 10 pg of the total RH strain T. g DNA. CONCLUSION: ISH was applicable in detecting pathogens in the lymph node tissues of individuals with Toxoplasma infection.
Assuntos
DNA de Protozoário/análise , Linfonodos/parasitologia , Toxoplasma/isolamento & purificação , Toxoplasmose/parasitologia , Animais , Doença de Hodgkin/parasitologia , Humanos , Hibridização In Situ , Linfoma não Hodgkin/parasitologiaRESUMO
Hodgkin's disease (HD) is a specific type of malignant lymphoma characteristic of local and general lymphadenectasis. Aquired toxoplasmosis (AT) is one kind of lymphoadenopathy without fever and fatigue. When the two diseases coexist, clinical and pathological misdiagnosis may be made. This is the first male case of toxoplasmosis and Hodgkin's disease in China, diagnosed by surgical removal of the major part of the cervical and supraclavicular masses, detection of blood anti-toxoplasma gondii antibody, PCR analysis of toxoplasma gondii DNA, and pathological, ultrastructural and immunohistochemical studies of the tumour tissues. The patient treated by radiation and chemotherapy was abated.
Assuntos
Doença de Hodgkin/parasitologia , Toxoplasmose/terapia , Adulto , Terapia Combinada , Doença de Hodgkin/terapia , Humanos , MasculinoRESUMO
The autopsy of a man who died of Hodgkin disease revealed that a peculiar metazoan parasite had proliferated and disseminated throughout his body. The parasite could not be identified; however, electron microscopical studies revealed that it had the structure of a flatworm. This, together with its shape and structure, convinced us that the parasite was an aberrant sparganum manifesting uncontrolled proliferation and dissemination.