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1.
Ecotoxicol Environ Saf ; 206: 111397, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33007538

RESUMO

During the harvest period, tobacco workers are exposed to nicotine and it is known that absorption of the alkaloid via the leaves causes green tobacco sickness (GST). We investigated if GST and its symptoms are associated with DNA damage and alterations of the redox status. DNA damage was measured in lymphocytes of tobacco workers and controls (n = 40/group) in single cell gel electrophoresis assays. Exposure to nicotine was determined by plasma cotinine measurements, alterations of the redox status by quantification of the total antioxidant capacity (TEAC) and of thiobarbituric acid reactive substances (TBARS). The symptoms of GTS included nausea, abdominal cramps, headache, vomiting and dizziness, and 50% of the workers had more than one symptom. Cotinine levels were enhanced in the workers (111 ng/mL); furthermore, the extent of DNA damage was ca. 3-fold higher than in the controls. This effect was more pronounced in participants with GST compared to healthy nicotine exposed workers and increased in individuals with specific symptoms (range 22-36%). TBARS levels did not differ between workers and unexposed controls, while TEAC values were even increased (by 14.3%). Contact with nicotine present in tobacco leaves causes GTS and leads to damage of the DNA; this effect is more pronounced in workers with GTS symptoms and is associated with alterations of the redox status. Damage of the genetic material which was found in the workers may lead to adverse long-term effects that are caused by genomic instability such as cancer and accelerated ageing.


Assuntos
Doenças dos Trabalhadores Agrícolas/induzido quimicamente , Dano ao DNA , Fazendeiros , Nicotiana/crescimento & desenvolvimento , Nicotina/toxicidade , Exposição Ocupacional/efeitos adversos , Estresse Oxidativo/efeitos dos fármacos , Adulto , Doenças dos Trabalhadores Agrícolas/genética , Doenças dos Trabalhadores Agrícolas/metabolismo , Brasil , Estudos de Casos e Controles , Cotinina/sangue , Feminino , Instabilidade Genômica/efeitos dos fármacos , Humanos , Masculino , Nicotina/metabolismo , Exposição Ocupacional/análise , Oxirredução , Estresse Oxidativo/genética , Substâncias Reativas com Ácido Tiobarbitúrico/análise , Nicotiana/metabolismo , Adulto Jovem
2.
Arch Environ Occup Health ; 67(1): 22-30, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22315932

RESUMO

Pesticides are frequently used substances worldwide, even when the use of some of them is forbidden due to the recognized adverse effect they have on the health of not only the people who apply the pesticides, but also of those that consume the contaminated products. The objectives of this study were to know the health issues of farm workers chronically exposed to pesticides, to evaluate possible damage at genetic level, as well as to explore some hepatic, renal, and hematological alterations. A transversal comparative study was performed between 2 groups, one composed of 25 farm workers engaged in pesticide spraying, and a control group of 21 workers not exposed to pesticides; both groups belonged to the Nextipac community in Jalisco, Mexico. Each member of both groups underwent a full medical history. Blood samples were taken from all farm workers in order to obtain a complete blood count and chemistry, clinical chemistry, lipid profile, liver and kidney function tests, erythrocyte cholinesterase quantification, lipid peroxidation profile, and free DNA fragment quantification. For the information analysis, central tendency and dispersion measurements were registered. In order to know the differences between groups, a cluster multivariate method was used, as well as prevalence reasons. The most used pesticides were mainly organophosphates, triazines and organochlorine compounds. The exposed group showed acute poisoning (20% of the cases) and diverse alterations of the digestive, neurological, respiratory, circulatory, dermatological, renal, and reproductive system probably associated to pesticide exposure. More importantly, they presented free DNA fragments in plasma (90.8 vs 49.05 ng/mL) as well as a higher level of lipid peroxidation (41.85 vs. 31.91 nmol/mL) in comparison with those data from unexposed farm workers. These results suggest that there exist health hazards for those farm workers exposed to pesticides, at organic and cellular levels.


Assuntos
Doenças dos Trabalhadores Agrícolas/induzido quimicamente , Fragmentação do DNA , Herbicidas/toxicidade , Inseticidas/toxicidade , Peroxidação de Lipídeos , Exposição Ocupacional/efeitos adversos , Adulto , Doenças dos Trabalhadores Agrícolas/genética , Doenças dos Trabalhadores Agrícolas/metabolismo , Agroquímicos/toxicidade , Estudos de Casos e Controles , Colinesterases/metabolismo , Análise por Conglomerados , Estudos Transversais , Eritrócitos/enzimologia , Humanos , Testes de Função Renal , Testes de Função Hepática , Masculino , México , Pessoa de Meia-Idade , Análise Multivariada , Exposição Ocupacional/análise , Organofosfatos/toxicidade
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