Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Doença de Chagas/epidemiologia , Doença de Chagas/etnologia , Doença de Chagas/prevenção & controle , Marca-Passo Artificial , Marca-Passo Artificial , Cardiomiopatias/complicações , Cardiomiopatias/diagnóstico , Epidemiologia Molecular/classificação , Nó Sinoatrial/lesõesRESUMO
We have identified a set of indicators to track usage patterns of ontologies developed using the semantically explicit S3DB model (www.s3db.org, Nature Biotechnology (2006) 24(9):1070-1071). These indicators are quantitative measures that assess the maturity of the data model. As a case study, we have allowed experimental researchers working on molecular epidemiology to incubate a data model for molecular epidemiology by restructuring the underlying ontology and evaluating it with real data.
Assuntos
Epidemiologia Molecular/classificação , Vocabulário Controlado , Staphylococcus aureus/genéticaRESUMO
Phenotypic and genetic characteristics of 21 strains of Salmonella serotype Heidelberg isolated in the years 1999-2003 from different sources in Italy were studied. Susceptibility patterns, plasmid analysis, and PFGE were used as epidemiological markers. Although non-homogeneous drug resistance patterns and plasmid profiles had been detected, PFGE patterns suggest the hypothesis of a nationwide clonal spread of this serotype associated with poultry.
Assuntos
Infecções por Salmonella/epidemiologia , Infecções por Salmonella/genética , Salmonella enterica/classificação , Salmonella enterica/isolamento & purificação , Animais , Galinhas/microbiologia , Criança , DNA Bacteriano/classificação , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla , Eletroforese em Gel de Campo Pulsado/métodos , Genótipo , Humanos , Itália/epidemiologia , Masculino , Testes de Sensibilidade Microbiana/métodos , Epidemiologia Molecular/classificação , Epidemiologia Molecular/métodos , Fenótipo , Vigilância da População/métodos , Intoxicação Alimentar por Salmonella/epidemiologia , Infecções por Salmonella/classificação , Infecções por Salmonella/metabolismo , Salmonella enterica/efeitos dos fármacos , Salmonella enterica/genética , SorotipagemRESUMO
Borrelia burgdorferi sensu lato (s. l.), the etiological agent of Lyme diesease, is transmitted by the bite of Ixodes ricinus. During May and September 1999, field surveys on Lyme disease spirochetes were conducted in three locations of a region of north-west Poland, known as recreational districts visited by many people. The ticks Ixodes ricinus were collected in natural habitats by dragging a flanel cloth over the vegetation. Sex and developmental stage of each tick were determined. Based on a polymerase chain reaction test with primers that recognize a chromosomal gene of all strains, out of the total 1414 specimens collected, 126 (8.9%) were found to be infected. The species B. burgdorferi s. l. comprises at least three pathogenic genomospecies, B. burgdorferi sensu stricto (s. s.), Borrelia garinii, and Borerelia afzelii, witch could be distinguished in nested-PCR tests with species-specific primers. B. burgdorferi s. s. was most prevalent (96% of infected ticks), followed by B. garinii (1.3%), and B. afzelii. was not found. Of the infected ticks, over the 99% were infected with a single species, one specimens was infected with two species. For 4 ticks, the infecting species could not be identied. The difference in rates of prevalence was observed among the tree locations (17%--5.3%--3.2%).
