RESUMO
Seven different polyamine-linked Sepharose derivatives were prepared for the affinity chromatography of spermidine and spermine binding macromolecules: Spermine synthase from rat and hog brain was used as a model protein with a spermidine binding site. Comparative studies of the affinities of the enzymes for the seven matrixes suggested that two negative charges, three to four methylene groups apart, should be present at the decarboxylated S-adenosylmethionine binding site and should improve the binding of the enzyme to the Sepharose derivative. Two negative charges at the spermidine binding site would be expected to do the same. Three affinity matrixes linked with 1,17-diamino-4,9,14-triazaheptadecane, 1,21-diamino-4,9,13,18-tetraazaheneicosane, and 5-spermine carboxylic acid, respectively, had an affinity for spermine synthases higher than that of spermine-Sepharose, which has been used for the purification of spermine synthase. The first of these matrixes was used and proved to be effective for the purification.
Assuntos
Sefarose/análogos & derivados , Sefarose/isolamento & purificação , Espermina Sintase/isolamento & purificação , Animais , Sítios de Ligação , Encéfalo/enzimologia , Proteínas de Transporte/química , Proteínas de Transporte/isolamento & purificação , Cromatografia de Afinidade , Poliaminas , Ratos , Espermina Sintase/química , SuínosRESUMO
Spermidine synthase was purified to apparent homogeneity from human spleens (8700-fold) by affinity chromatography. The native enzyme was composed of two subunits of identical Mr (35,000) and showed an apparent Mr of 62,000 in pore-gradient gel electrophoresis. Its pI was 5.1, Spermine synthase was purified to apparent homogeneity from placenta (5300-fold) and from kidney (4600-fold). The native enzyme was composed of two subunits of identical Mr (45,000) and showed an apparent Mr of 78,000 in pore-gradient gel electrophoresis. In isoelectric focusing it revealed two bands, with pI values of 4.9 and 5.0. Both synthases were present in all human tissues studied, but revealed a clear tissue-specific pattern. Mouse antisera against spermidine synthase revealed only one band, of Mr 35,000, in all purified enzyme preparations and in crude human tissue extracts in immunoblotting. Antisera against spermine synthase showed an immunoreactive band corresponding to the Mr of the subunit of spermine synthase. These antisera did not indicate any cross-reactivity in immunoblotting. Thus spermine synthase and spermidine synthase do not share homologous antigenic sites and are totally different proteins.
Assuntos
Espermidina Sintase/isolamento & purificação , Espermina Sintase/isolamento & purificação , Transferases/isolamento & purificação , Western Blotting , Cromatografia de Afinidade , Eletroforese em Gel de Poliacrilamida , Humanos , Focalização Isoelétrica , Cinética , Espermidina Sintase/metabolismo , Espermina Sintase/metabolismoRESUMO
Spermidine synthase from rat ventral prostate was inhibited by 2-mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S-adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ.
Assuntos
Cisteamina/farmacologia , Próstata/enzimologia , Espermidina Sintase/antagonistas & inibidores , Transferases/antagonistas & inibidores , Animais , Ligação Competitiva , Linhagem Celular , Cinética , Leucemia Mieloide , Masculino , Ratos , Ratos Endogâmicos , Espermidina Sintase/isolamento & purificação , Espermina Sintase/isolamento & purificação , Espermina Sintase/metabolismoAssuntos
Espermidina Sintase/genética , Transferases/genética , Sequência de Aminoácidos , Animais , Encéfalo/enzimologia , Bovinos , Humanos , Immunoblotting , Dados de Sequência Molecular , Mapeamento de Peptídeos , Espermidina Sintase/análise , Espermidina Sintase/biossíntese , Espermidina Sintase/isolamento & purificação , Espermina Sintase/análise , Espermina Sintase/genética , Espermina Sintase/isolamento & purificação , Timo/enzimologiaAssuntos
Encéfalo/enzimologia , Espermina Sintase/isolamento & purificação , Transferases/isolamento & purificação , Sulfato de Amônio , Animais , Bovinos , Cromatografia de Afinidade/métodos , Cromatografia DEAE-Celulose/métodos , Cinética , Peso Molecular , Espermina Sintase/antagonistas & inibidores , Especificidade por SubstratoRESUMO
Spermine synthase, a propylamine transferase, which catalyses the biosynthesis of spermine from S-methyladenosylhomocystemine and spermidine has been purified to an apparent homogeneity (about 6000-fold) from bovine brain using spermine-Sepharose affinity chromatography. The enzyme preparation was free from S-adenosylmethionine decarboxylase and spermidine synthase activities. The molecular Stokes radius of the enzyme was calculated to be 4.16 nm. The enzyme has an apparent molecular weight of approximately 88 000, composing of two subunits of equal size. The enzyme showed a broad pH optimum between 7.0 and 8.0 and an acidic isoelectric point at pH 5.10. The apparent Km values for S-methyladenosylhomocysteamine was 0.6 microM and about 60 microM for spermidine. The enzyme showed strict specificity to spermidine as the propylamine acceptor. Both the reaction products, spermine and 5'-methylthioadenosine inhibited the enzyme activity, methylthioadenosine being a powerful competitive inhibitor with respect to S-methyladenosylhomocysteamine (Ki value of about 0.3 microM). Putrescine also inhibited competitively with respect to spermidine (Ki value of about 1.7 mM). Spermine synthase had no requirements for metal or other cofactors.