RESUMO
A broad coverage influenza vaccine against multiple viral strains based on the viral nucleoprotein (NP) is a goal pursued by many laboratories. If the goal is to formulate the vaccine with recombinant NP it is essential to count on adjuvants capable of inducing cellular immunity. This work have studied the effect of the monophosphoryl lipid A and trehalose dimycolate, known as the Ribi Adjuvant System (RAS), in the immune response induced in mice immunized with recombinant NP. The NP was formulated with RAS and used to immunize BALB/c mice. Immunizations with NP-RAS increased the humoral and cellular immune responses compared to unadjuvanted NP. The predominant antibody isotype was IgG2a, suggesting the development of a Th1 response. Analysis of the cytokines from mice immunized with NP-RAS showed a significant increase in the production of IFN-g and a decreased production of IL-10 and IL-4 compared to controls without RAS. These results are similar to those usually obtained using Freund's adjuvant, known to induce Th1 and CTL responses when co-administered with purified proteins, and suggest that a similar approach may be possible to enhance the performance of a T-cell vaccine containing NP.
Assuntos
Esqueleto da Parede Celular/administração & dosagem , Fatores Corda/administração & dosagem , Influenza Humana/imunologia , Lipídeo A/análogos & derivados , Proteínas de Ligação a RNA/imunologia , Células Th1/imunologia , Proteínas do Core Viral/imunologia , Animais , Anticorpos Antivirais/imunologia , Esqueleto da Parede Celular/imunologia , Fatores Corda/imunologia , Feminino , Humanos , Imunidade Celular , Imunização , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/genética , Vacinas contra Influenza/imunologia , Influenza Humana/prevenção & controle , Influenza Humana/virologia , Interferon gama/imunologia , Interleucina-10/imunologia , Interleucina-4/imunologia , Lipídeo A/administração & dosagem , Lipídeo A/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Proteínas do Nucleocapsídeo , Proteínas de Ligação a RNA/administração & dosagem , Proteínas de Ligação a RNA/genética , Linfócitos T Citotóxicos/imunologia , Proteínas do Core Viral/administração & dosagem , Proteínas do Core Viral/genéticaRESUMO
Only native products of Mycobacterium leprae, whether cell wall, cytosol, or membrane derived, can confer protective immunity against challenge in the mouse footpad. Previously, recombinant proteins were shown to be ineffective. The cell wall skeleton-the mycolyl-arabinogalactan-peptidoglycan complex-devoid of proteins is not protective.