RESUMO
C-Glycosyltransferases (CGTs) catalyze the formation of C-glycosidic bonds for the biosynthesis of C-glycosides, but the underlying mechanism is unclear. This process improves the solubility and bioavailability of specialized metabolites, which play important roles in plant growth and development and represent rich resources for drug discovery. Here, we performed functional and structural studies of the CGT UGT708C1 from buckwheat (Fagopyrum esculentum). Enzymatic analysis showed that UGT708C1 is capable of utilizing both UDP-galactose and UDP-glucose as sugar donors. Our structural studies of UGT708C1 complexed with UDP-glucose and UDP identified the key roles of Asp382, Gln383, Thr151, and Thr150 in recognizing the sugar moiety of the donor substrate and Phe130, Tyr102, and Phe198 in binding and stabilizing the acceptor. A systematic site-directed mutagenesis study confirmed the important roles of these residues. Further structural analysis combined with molecular dynamics simulations revealed that phloretin binds to the acceptor binding pocket in a bent state with a precise spatial disposition and complementarity. These findings provide insights into a catalytic mechanism for CGTs.
Assuntos
Fagopyrum/enzimologia , Glicosiltransferases/química , Glicosiltransferases/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Sítios de Ligação , Cristalografia por Raios X , Glicosilação , Glicosiltransferases/genética , Cinética , Modelos Moleculares , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Mutação , Floretina/metabolismo , Proteínas de Plantas/genética , Açúcares/química , Açúcares/metabolismoRESUMO
BACKGROUND: Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynthesis of flavonoids in plants. So far, few studies are available on UFGT genes that may play a role in tartary buckwheat flavonoids biosynthesis. Here, we report on the identification and functional characterization of seven UFGTs from tartary buckwheat that are potentially involved in flavonoid biosynthesis (and have varying effects on plant growth and development when overexpressed in Arabidopsis thaliana.) RESULTS: Phylogenetic analysis indicated that the potential function of the seven FtUFGT proteins, FtUFGT6, FtUFGT7, FtUFGT8, FtUFGT9, FtUFGT15, FtUFGT40, and FtUFGT41, could be divided into three Arabidopsis thaliana functional subgroups that are involved in flavonoid biosynthesis of and anthocyanin accumulation. A significant positive correlation between FtUFGT8 and FtUFGT15 expression and anthocyanin accumulation capacity was observed in the tartary buckwheat seedlings after cold stress. Overexpression in Arabidopsis thaliana showed that FtUFGT8, FtUFGT15, and FtUFGT41 significantly increased the anthocyanin content in transgenic plants. Unexpectedly, overexpression of FtUFGT6, while not leading to enhanced anthocyanin accumulation, significantly enhanced the growth yield of transgenic plants. When wild-type plants have only cotyledons, most of the transgenic plants of FtUFGT6 had grown true leaves. Moreover, the growth speed of the oxFtUFGT6 transgenic plant root was also significantly faster than that of the wild type. At later growth, FtUFGT6 transgenic plants showed larger leaves, earlier twitching times and more tillers than wild type, whereas FtUFGT15 showed opposite results. CONCLUSIONS: Seven FtUFGTs were isolated from tartary buckwheat. FtUFGT8, FtUFGT15, and FtUFGT41 can significantly increase the accumulation of total anthocyanins in transgenic plants. Furthermore, overexpression of FtUFGT6 increased the overall yield of Arabidopsis transgenic plants at all growth stages. However, FtUFGT15 shows the opposite trend at later growth stage and delays the growth speed of plants. These results suggested that the biological function of FtUFGT genes in tartary buckwheat is diverse.
Assuntos
Fagopyrum/genética , Genes de Plantas/genética , Glicosiltransferases/genética , Proteínas de Plantas/genética , Antocianinas/metabolismo , Arabidopsis/genética , Sequência Conservada , Fagopyrum/enzimologia , Flavonoides/metabolismo , Genes de Plantas/fisiologia , Glicosiltransferases/fisiologia , Filogenia , Proteínas de Plantas/fisiologia , Plantas Geneticamente Modificadas , Análise de Sequência de DNARESUMO
ß-Glucosidase (BG) was immobilized on the surface of bifunctionalized nano-magnetic iron oxide with silica and amine groups (Fe3O4@SiO2-NH2). The aroma and flavonoid aglycone enhancement effect of BG in tea soup was investigated. The immobilized BG-synthesized nanocomposite morphology and structure were characterized by using different analytical techniques, including Fourier transform infrared spectroscopy and scanning electron microscopy. The immobilized BG showed enhanced pH and temperature endurance at an optimum pH of 5.0 and temperature of 65 °C. After seven cycles of reuse, immobilized BG showed 51.8% initial activity. Immobilized-BG treatment in green tea and black tea soup elevated the aroma content by approximately 16% and 48%, respectively. In addition, flavonoid aglycones, such as myricetin, kaempferol, and quercetin, in green tea and black tea soup increased by approximately 65- and 5-fold, respectively. These results suggested that immobilized BG showed excellent potential in the enhancement of aroma and effectively hydrolyzed the flavonoid glycosides to release flavonoid aglycones in tea soup. Hence, this study provides a green and sustainable approach for the tea industry to efficiently enhance tea soup properties.
Assuntos
Fagopyrum/enzimologia , Flavonoides/química , Proteínas de Plantas/química , Chá/química , beta-Glucosidase/química , Biocatálise , Enzimas Imobilizadas/química , Compostos Férricos/química , Concentração de Íons de Hidrogênio , Odorantes/análiseRESUMO
Anthocyanins are a group of flavonoids found in buckwheat (Fagopyrum esculentum) and many other plant species; however; little is known about their mechanisms of synthesis and regulation in buckwheat. We previously reported a spontaneous mutant buckwheat line that shows the green stem phenotype; this line does not accumulate anthocyanins but synthesizes flavonol and proanthocyanidin in the stem. Here, we used this line and lines developed by this line to search for genes related to anthocyanin accumulation in buckwheat. The lines with green stem showed flavonoid-3-O-glucosyltransferase activity against UDP-glucose, indicating that the flavonoid-3-O-glucosyltransferase gene was not controlling the green stem trait. We therefore searched the buckwheat genome database for a gene encoding glutathione S-transferase (GST), a flavonoid-binding protein that transports flavonoids to the vacuole, and identified a candidate gene, FeGST1. Expression analysis showed that FeGST1 was expressed in wild type buckwheat but not in the green stem lines. Linkage analysis with an F2 segregating population produced by crossing between the green stem line and a self-compatible line showed that FeGST1 segregated with stem color without any recombination. This indicates that the green stem trait could be caused by homozygous non-functional alleles of the FeGST1 locus.
Assuntos
Antocianinas/metabolismo , Fagopyrum/genética , Genes de Plantas/genética , Glutationa Transferase/genética , Proteínas de Plantas/genética , Fagopyrum/enzimologia , Fagopyrum/metabolismo , Ligação Genética , Glutationa Transferase/metabolismo , Proteínas de Plantas/metabolismo , Caules de Planta/metabolismo , Característica Quantitativa HerdávelRESUMO
Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6â³-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6â³RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6â³RhaT enzyme expressed in Escherichia coli exhibited 6â³-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6â³RhaT converted the administered quercetin into rutin, suggesting that FeF3G6â³RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6â³RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6â³RhaT is involved in rutin biosynthesis in common buckwheat.
Assuntos
Fagopyrum/metabolismo , Hexosiltransferases/metabolismo , Rutina/biossíntese , Cromatografia Líquida de Alta Pressão , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Fagopyrum/enzimologia , Hexosiltransferases/genética , Hexosiltransferases/isolamento & purificação , Fenóis/metabolismo , Reação em Cadeia da Polimerase , Plântula/enzimologia , Análise de Sequência de RNA , Especificidade por SubstratoRESUMO
Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F.tataricum exhibited good functions for free radicals scavengingï¼ anti-oxidationï¼ anti-aging activities. Although much genetic knowledge of the synthesisï¼ regulationï¼ accumulation of rutinï¼ the genetic basis of proanthocyanidins(PAs) in tartary buckwheat and their related gene expression changes under different lights(blueï¼ redï¼ far redï¼ ultraviolet light) remain largely unexplored. In this studyï¼ we cloned one anthocyanidin reductase gene(ANR) and two leucocyanidin reductase gene(LAR) named FtANRï¼FtLAR1ï¼FtLAR3 involved in formation of(+)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of FtANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red lightï¼ while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.
Assuntos
Fagopyrum/enzimologia , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Luz , Proantocianidinas/biossíntese , Fagopyrum/efeitos da radiação , NADH NADPH Oxirredutases/genética , Proteínas de Plantas/genética , Sementes/enzimologia , Sementes/efeitos da radiaçãoRESUMO
Five new geminal aminocycloalkanephosphonic acids (4 - 8) containing both an aromatic ring and a cycloalkane ring were synthesized and evaluated as potential inhibitors of buckwheat phenylalanine ammonia-lyase (PAL). Within the set of compounds which are related to 2-aminoindane-2-phosphonic acid (AIP, 3), a known powerful inhibitor of PAL, racemic 1-aminobenzocyclobutene-1-phosphonic acid (4), was six times weaker than AIP as an in vitro inhibitor of buckwheat PAL, but six times stronger than AIP as an in vivo inhibitor of phenylalanine-derived anthocyanin synthesis in buckwheat.
Assuntos
Fenilalanina Amônia-Liase/antagonistas & inibidores , Ácidos Fosforosos/síntese química , Ácidos Fosforosos/farmacologia , Antocianinas/antagonistas & inibidores , Antocianinas/biossíntese , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/farmacologia , Fagopyrum/enzimologia , IndanosRESUMO
Proanthocyanidins (PAs) are a major group of flavonoids synthesized via the phenylpropanoid biosynthesis pathway, however the pathway has not been fully characterized in buckwheat. Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are involved in the last steps of PA biosynthesis. To isolate the genes for these enzymes from buckwheat we performed PCR using degenerate primers and obtained cDNAs of ANR and LAR, which we designated FeANR and FeLAR1. A search for homologs in a buckwheat genome database with both sequences returned two more LAR sequences, designated FeLAR2 and FeLAR3. Linkage analysis with an F2 segregating population indicated that the three LAR loci were not genetically linked. We detected high levels of PAs in roots and cotyledons of buckwheat seedlings and in buds and flowers of mature plants. FeANR and FeLAR1-3 were expressed in most organs but had different expression patterns. Our findings would be useful for breeding and further analysis of PA synthesis and its regulation in buckwheat.
Assuntos
Antocianinas/metabolismo , Fagopyrum/enzimologia , Oxirredutases/genética , Proantocianidinas/metabolismo , Vias Biossintéticas , Cruzamento , Cotilédone/enzimologia , Cotilédone/genética , DNA Complementar/genética , Fagopyrum/genética , Flores/enzimologia , Flores/genética , Regulação da Expressão Gênica de Plantas , Loci Gênicos/genética , Oxirredutases/metabolismo , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Raízes de Plantas/enzimologia , Raízes de Plantas/genética , Plântula/enzimologia , Plântula/genética , Análise de Sequência de DNARESUMO
Anthocyanins confer the red color in the hypocotyl of tartary buckwheat sprouts. Uridine diphosphate (UDP)-glucose:flavonoid 3-O-glycosyltransferase (UFGT) stabilizes anthocyanin by attaching the glucosyl moiety from UDP-glucose to the C3 hydroxyl of anthocyanin. In this study, we characterized three UFGT-like genes, designated FtUFGT1, 2, and 3 from tartary buckwheat. The results revealed that FtUFGT1, FtUFGT2, and FtUFGT3 can convert cyanidin to cyanidin 3-O-glucoside, with specific activities of 20.01 × 10(-3), 8.93 × 10(-3), and 20.24 × 10(-3) IU/mg, respectively. The active-site residues of the C-terminal domains and the N-terminal domains are important for the donor and acceptor recognition of these proteins. The expression of the three FtUFGTs paralleled the tissue-specific anthocyanin accumulation. After cold treatment, the increased content of anthocyanin was accompanied by the up-regulated expression of the three FtUFGTs. Among these three UGFT gene members, FtUFGT3 showed the highest expression level and the highest specific activity, suggesting that FtUFGT3 might be the major gene involved in anthocyanin biosynthesis. These results suggested that the FtUFGT genes, FtUFGT3 in particular, might be important candidates for anthocyanin formation in tartary buckwheat sprouts.
Assuntos
Fagopyrum/enzimologia , Glucosiltransferases/genética , Proteínas de Plantas/genética , Antocianinas/metabolismo , Temperatura Baixa , Fagopyrum/genética , Fagopyrum/crescimento & desenvolvimento , Fagopyrum/metabolismo , Regulação da Expressão Gênica de Plantas , Glucosiltransferases/metabolismo , Proteínas de Plantas/metabolismo , Sementes/enzimologia , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Estresse FisiológicoRESUMO
Phenylalanine Ammonia Lyase (PAL) gene which plays a key role in bio-synthesis of medicinally important compounds, Rutin/quercetin was sequence characterized for its efficient genomics application. These compounds possessing anti-diabetic and anti-cancer properties and are predominantly produced by Fagopyrum spp. In the present study, PAL gene was sequenced from three Fagopyrum spp. (F. tataricum, F. esculentum and F. dibotrys) and showed the presence of three SNPs and four insertion/deletions at intra and inter specific level. Among them, the potential SNP (position 949th bp G>C) with Parsimony Informative Site was selected and successfully utilised to individuate the zygosity/allelic variation of 16 F. tataricum varieties. Insertion mutations were identified in coding region, which resulted the change of a stretch of 39 amino acids on the putative protein. Our Study revealed that autogamous species (F. tataricum) has lower frequency of observed SNPs as compared to allogamous species (F. dibotrys and F. esculentum). The identified SNPs in F. tataricum didn't result to amino acid change, while in other two species it caused both conservative and non-conservative variations. Consistent pattern of SNPs across the species revealed their phylogenetic importance. We found two groups of F. tataricum and one of them was closely related with F. dibotrys. Sequence characterization information of PAL gene reported in present investigation can be utilized in genetic improvement of buckwheat in reference to its medicinal value.
Assuntos
Fagopyrum/genética , Genoma de Planta , Fenilalanina Amônia-Liase/genética , Proteínas de Plantas/genética , Polimorfismo de Nucleotídeo Único , Fagopyrum/enzimologiaRESUMO
A ribonuclease, with a molecular mass of 22.5 kDa and an N-terminal sequence exhibiting resemblance to previously isolated buckwheat storage proteins and allergens, was isolated from Japanese large brown buckwheat seeds. The ribonuclease was purified using a simple protocol that comprised ion exchange chromatography on Q-Sepharose and DEAE-cellulose and gel filtration on Superdex 75. The ribonuclease exhibited low activity toward poly U, lower activity toward poly C, and very low activity toward poly A and poly G. The enzyme was activated upon exposure to 10 mM of Fe(2+) and Zn(2+) ions but was inhibited by Ca(2+), Mg(2+), and Mn(2+) ions at the same concentration. The optimum pH and optimum temperature for the enzyme were pH 9 and 60 °C, respectively. It inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 79.2 and 63.8 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 48 µM. However, there were no antifungal and mitogenic activities.
Assuntos
Fagopyrum/enzimologia , Transcriptase Reversa do HIV/antagonistas & inibidores , HIV-1/enzimologia , Proteínas de Plantas/farmacologia , Ribonucleases/farmacologia , Sequência de Aminoácidos , Linhagem Celular Tumoral , Ativação Enzimática , Estabilidade Enzimática , Fagopyrum/química , Fagopyrum/genética , Transcriptase Reversa do HIV/química , Transcriptase Reversa do HIV/metabolismo , HIV-1/efeitos dos fármacos , Humanos , Dados de Sequência Molecular , Peso Molecular , Proteínas de Plantas/química , Proteínas de Plantas/isolamento & purificação , Proteínas de Plantas/metabolismo , Ribonucleases/química , Ribonucleases/isolamento & purificação , Ribonucleases/metabolismo , Sementes/química , Sementes/enzimologia , Sementes/genéticaRESUMO
C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids; however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentum M. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2',4',6'-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases.
Assuntos
Fagopyrum/enzimologia , Flavonoides/metabolismo , Glucosiltransferases/metabolismo , Sequência de Bases , Clonagem Molecular , Cotilédone/enzimologia , Cotilédone/genética , DNA Complementar/genética , Fagopyrum/genética , Glucosiltransferases/genética , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plântula/enzimologia , Plântula/genética , Análise de Sequência de DNARESUMO
Ribonucleases (RNases) are present in base-level amounts in intact plants, but this level is able to increase greatly under stress conditions. The possible cause for such an increase is protection against plant RNA-virus attack. Buckwheat burn virus (BBV) is a highly virulent pathogen that belongs to Rhabdoviridae family. In our study, we have analyzed the correlation between RNase activity and resistance of different buckwheat cultivars to BBV infection. Two cultivars, Kara-Dag and Roksolana, with different sensitivities to BBV have been used. Kara-Dag is a cultivar with medium sensitivity to virus and Roksolana is a tolerant cultivar. It has been shown that the base level of RNase activity in Roksolana cultivar was in most cases higher than the corresponding parameter in Kara-Dag cultivar. Both infected and uninfected plants of Roksolana cultivar demonstrated high RNase activity during two weeks. Whereas infected plants of Kara-Dag cultivar demonstrated unstable levels of RNase activity. Significant decline in RNase activity was detected on the 7th day post infection with subsequent gradual increase in RNase activity. Decline of the RNase activity during the first week could promote the virus replication and therefore more successful infection of upper leaves of plants. Unstable levels of RNase activity in infected buckwheat plants may be explained by insufficiency of virus-resistant mechanisms that determines the medium sensitivity of the cultivar to BBV. Thus, plants of buckwheat cultivar having less sensitivity to virus, displayed in general higher RNase activity.
Assuntos
Fagopyrum/imunologia , Folhas de Planta/imunologia , Proteínas de Plantas/metabolismo , Ribonucleases/metabolismo , Fagopyrum/enzimologia , Fagopyrum/virologia , Interações Hospedeiro-Patógeno , Doenças das Plantas , Imunidade Vegetal , Folhas de Planta/enzimologia , Folhas de Planta/virologia , Proteínas de Plantas/imunologia , Rhabdoviridae/patogenicidade , Rhabdoviridae/fisiologia , Ribonucleases/imunologiaRESUMO
Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) play an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. The aim of this study was to clone ANR and LAR genes involved in PAs biosynthesis and examine the expression of these two genes in different organs under different growth conditions in two tartary buckwheat cultivars, Hokkai T8 and T10. Gene expression was carried out by quantitative real-time RT-PCR, and catechin and epicatechin content was analyzed by high performance liquid chromatography. The expression pattern of ANR and LAR did not match the accumulation pattern of PAs in different organs of two cultivars. Epicatechin content was the highest in the flowers of both cultivars and it was affected by light in only Hokkai T8 sprouts. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions.
Assuntos
Antocianinas/metabolismo , Vias Biossintéticas/genética , Catequina/análise , Fagopyrum/enzimologia , NADH NADPH Oxirredutases/genética , Sequência de Bases , Catequina/biossíntese , Cromatografia Líquida de Alta Pressão , Clonagem Molecular , Biologia Computacional , DNA Complementar/genética , Fagopyrum/química , Dados de Sequência Molecular , Estrutura Molecular , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is increasingly considered as an important functional food material because of its rich nutraceutical compounds. Reserve starch is the major component of tartary buckwheat seed. However, the gene sequences and the molecular mechanism of tartary buckwheat starch synthesis are unknown so far. In this study, the complete genomic sequence and full-size cDNA coding tartary buckwheat granule-bound starch synthase I (FtGBSSI), which is responsible for amylose synthesis, were isolated and analyzed. The genomic sequence of the FtGBSSI contained 3947 nucleotides and was composed of 14 exons and 13 introns. The cDNA coding sequence of FtGBSSI shared 63.3%-75.1% identities with those of dicots and 56.6%-57.5% identities with monocots (Poaceae). In deduced amino acid sequence of FtGBSSI, eight motifs conserved among plant starch synthases were identified. A cleavage at the site IVC↓G of FtGBSSI protein produces the chloroplast transit sequence of 78 amino acids and the mature protein of 527 amino acids. The FtGBSSI mature protein showed an identity of 73.4%-77.8% with dicot plants, and 67.6%-70.4% with monocot plants (Poaceae). The mature protein was composed of 20 α-helixes and 16 ß-strands, and folds into two main domains, N- and C-terminal domains. The critical residues which are involved in ADP and sugar binding were predicted. These results will be useful to modulate starch composition of buckwheat kernels with the aim to produce novel improved varieties in future breeding programs.
Assuntos
Fagopyrum/genética , Sintase do Amido/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular/métodos , DNA Complementar/genética , Fagopyrum/enzimologia , Dados de Sequência Molecular , Filogenia , Proteínas de Plantas/genética , Estrutura Terciária de Proteína , Alinhamento de Sequência , Amido/genéticaRESUMO
Rutin-degrading enzymes (RDEs) specifically hydrolyze the glycosidic linkages of rutin, producing quercetin and rutinose. Here we report a reliable and sensitive polyacrylamide gel electrophoresis and staining method for the detection of RDE isozymes, which is based on the aqueous solubility difference between rutin and quercetin, as well as the ultraviolet absorbance of quercetin. With this novel method, we achieved a detection limit of 12 ng with 107 U of RDE activity, enabling us to detect at least five RDE isozymes in tartary buckwheat seeds.
Assuntos
Eletroforese em Gel de Poliacrilamida/métodos , Ensaios Enzimáticos/métodos , Fagopyrum/enzimologia , Rutina/metabolismo , Western Blotting , Fagopyrum/metabolismo , Isoenzimas/isolamento & purificação , Isoenzimas/metabolismo , Limite de Detecção , Rutina/isolamento & purificaçãoRESUMO
Flavonoids are ubiquitously present in plants and play important roles in these organisms as well as in the human diet. Flavonol synthase (FLS) is a key enzyme of the flavonoid biosynthetic pathway, acting at the diverging point into the flavonol subclass branch. We isolated and characterized a FLS isoform gene, FtFLS2, from tartary buckwheat (Fagopyrum tataricum). FtFLS2 shares 48% identity and 67% similarity with the previously reported FtFLS1, whereas both genes share 47-65% identity and 65-69% similarity with FLSs from other plant species. Using quantitative real-time PCR and high-performance liquid chromatography (HPLC), the expression of FtFLS1/2 and the production of 3 main flavonols (kaempferol, myricetin and quercetin) was detected in roots, leaves, stems, flowers and different stages of developing seeds. The relationship between the expression of the 2 FLS genes and the accumulation of the 3 basic flavonols was analyzed in 2 tartary buckwheat cultivars. FtFLS1 and FtFLS2 exhibited differential transcriptional levels between the tartary buckwheat cultivars 'Hokkai T10' and 'Hokkai T8'. Generally, higher transcript levels of FtFLS1 and FtFLS2 and a higher amount of flavonols were observed in the 'Hokkai T10' cultivar than 'Hokkai T8'. The content of flavonols showed tissue-specific accumulation between the 2 cultivars. The transcription of FtFLS1 was inhibited by the exogenous application of abscisic acid (ABA), salicylic acid (SA) and sodium chloride (NaCl), while FtFLS2 was not affected by ABA but up-regulated by SA and NaCl. These data indicate that the 2 FtFLS isoforms of buckwheat have different functions in the response of buckwheat to environmental stress.
Assuntos
Fagopyrum/enzimologia , Fagopyrum/genética , Flavonóis/metabolismo , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Oxirredutases/genética , Proteínas de Plantas/genética , Estresse Fisiológico/genética , Sequência de Aminoácidos , Clonagem Molecular , Flavonoides/metabolismo , Flavonóis/química , Regulação Enzimológica da Expressão Gênica , Humanos , Quempferóis/metabolismo , Dados de Sequência Molecular , Oxirredutases/química , Oxirredutases/metabolismo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Quercetina/metabolismo , Plântula/genética , Alinhamento de SequênciaRESUMO
The rutin degrading enzyme (RDE) was isolated and purified from tartary buckwheat seeds. The RDE was purified about 11.34-fold and its final yield was 3.5%, which was very low, due to our purification strategy of giving priority to purity over yield. The RDE molecular weight was estimated to be about 60 kDa. When rutin was used as substrate, an optimal enzyme activity was seen at around pH 5.0 and 40 °C. Strains isolation strategy characterized by the use of rutin as sole carbon source in enrichment cultures was used to isolate RDE-producing strains. Then the active strains were identified by morphology characterization and 18s rDNA-ITS (Internal Transcribed Spacer) gene sequencing. Three isolates coded as B3, W2, Y2 were successfully isolated from fusty Fagopyrum tataricum flour cultures. Strain B3 possessed the highest unit activity among these three strains, and its total activity reached up to 171.0 Unit. The active isolate (B3) could be assigned to Penicillium farinosum. When the Penicillium farinosum strains were added to tartary buckwheat flour cultures at pH 5.0, 30 °C after 5 days fermentation, the quercetin production raised up to 1.78 mg/l, almost 5.1 times higher than the fermentation without the above active strains. Hence, a new approach was available to utilize microorganism-aided fermentation for effective quercetin extraction from Fagopyrum tataricum seeds.
Assuntos
Enzimas/isolamento & purificação , Enzimas/metabolismo , Fagopyrum/enzimologia , Rutina/metabolismo , Biotransformação , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Enzimas/química , Concentração de Íons de Hidrogênio , Peso Molecular , Penicillium/classificação , Penicillium/genética , Penicillium/isolamento & purificação , Sementes/enzimologia , Análise de Sequência de DNA , TemperaturaRESUMO
The leucoanthocyantin reducase (LAR) gene, an important functional gene of catechins biosynthesis pathway, was cloned from Fagopyrum dibotrys (D.Don) Hara by degenerate PCR and rapid amplification of cDNA ends (RACE). The full-length cDNA of FdLAR is 1 581 bp (GenBank accession: JN793953), containing a 1 176 bp ORF encoding a 391 amino acids protein, and its 3'-untranslated region has an obvious polyadenylation signal. The recombinant plasmid containing FdLAR completed ORF was transformed into E. coli BL21 (DE3). The target fusion peptide with molecular weight of 66 kD was expressed under the condition of 16 degrees C and induced by IPTG at final concentration of 1.0 mmol x L(-1). Bioinformation analysis indicated that the amino acid sequence of FdLAR showed great homology to other LAR with the NADB-Rossmann conversed domain in the N-terminus. Real-time quantitative PCR was used to detect the expression levels of FdLAR gene during different development periods. The determination of flavonoids contents in appropriate rhizomes showed that the relationship between FdLAR gene expression and the accumulation of flavonoids displayed different trends during vegetative growth and reproductive growth stages, suggesting that the FdLAR gene may be involved in the pathway of flavonoid metabolisms in Fagopyrum dibotrys.
Assuntos
Antocianinas/metabolismo , Fagopyrum/genética , Oxirredutases/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos , Clonagem Molecular , Fagopyrum/enzimologia , Fagopyrum/crescimento & desenvolvimento , Flavonoides/análise , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Dados de Sequência Molecular , Oxirredutases/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Plantas Medicinais/enzimologia , Plantas Medicinais/genética , Plantas Medicinais/crescimento & desenvolvimento , Rizoma/genéticaRESUMO
α-Glucosidase is in the glycoside hydrolase family 13 (13AG) and 31 (31AG). Only 31AGs can hydrate the D-glucal double bond to form α-2-deoxyglucose. Because 1,5-anhydrofructose (AF), having a 2-OH group, mimics the oxocarbenium ion transition state, AF may be a substrate for α-glucosidases. α-Glucosidase-catalyzed hydration produced α-glucose from AF, which plateaued with time. Combined reaction with α-1,4-glucan lyase and 13AG eliminated the plateau. Aspergillus niger α-glucosidase (31AG), which is stable in organic solvent, produced ethyl α-glucoside from AF in 80% ethanol. The findings indicate that α-glucosidases catalyze trans-addition. This is the first report of α-glucosidase-associated glucose formation from AF, possibly contributing to the salvage pathway of unutilized AF.