1.
J Biotechnol
; 130(4): 389-93, 2007 Jul 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17566582
RESUMO
In this study, we describe the development of a cost effective and highly productive cell-free protein synthesis system derived from Escherichia coli. Through the use of an optimal energy source and cell extract, approximately 1.3mg/mL of protein was generated from a single batch reaction at greatly reduced reagent costs. Compared to previously reported systems, the described method yields approximately 14-fold higher productivity per unit reagent cost making this cell-free synthesis technique a promising alternative for more efficient protein production.