RESUMO
Dopa decarboxylase (DDC) protein is involved in the synthesis of dopamine and serotonin. Here, we show that in the silkworm Bombyx mori, a novel DDC splicing variant is selectively expressed in the brain and subesophageal ganglia. In Drosophila melanogaster, a neuron-specific isoform of DDC is known to be alternatively spliced in a similar manner.
Assuntos
Bombyx/genética , Dopa Descarboxilase/genética , Proteínas de Insetos/genética , Regiões 5' não Traduzidas , Processamento Alternativo , Animais , Encéfalo/enzimologia , Clonagem Molecular , Dopa Descarboxilase/metabolismo , Gânglios dos Invertebrados/enzimologia , Regulação Enzimológica da Expressão Gênica , Proteínas de Insetos/metabolismo , Especificidade de Órgãos , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , RNA Mensageiro/genéticaRESUMO
In decapod crustaceans, molting is controlled by the pulsatile release of molt-inhibiting hormone (MIH) from neurosecretory cells in the X-organ/sinus gland (XO/SG) complex in the eyestalk ganglia (ESG). A drop in MIH release triggers molting by activating the molting gland or Y-organ (YO). Post-transcriptional mechanisms ultimately control MIH levels in the hemolymph. Neurotransmitter-mediated electrical activity controls Ca2+-dependent vesicular release of MIH from the SG axon terminals, which may be modulated by nitric oxide (NO). In green shore crab, Carcinus maenas, nitric oxide synthase (NOS) protein and NO are present in the SG. Moreover, C. maenas are refractory to eyestalk ablation (ESA), suggesting other regions of the nervous system secrete sufficient amounts of MIH to prevent molting. By contrast, ESA induces molting in the blackback land crab, Gecarcinus lateralis. Double-label immunofluorescence microscopy and quantitative polymerase chain reaction were used to localize and quantify MIH and NOS proteins and transcripts, respectively, in the ESG, brain, and thoracic ganglion (TG) of C. maenas and G. lateralis. In ESG, MIH- and NOS-immunopositive cells were closely associated in the SG of both species; confocal microscopy showed that NOS was localized in cells adjacent to MIH-positive axon terminals. In brain, MIH-positive cells were located in a small number of cells in the olfactory lobe; no NOS immunofluorescence was detected. In TG, MIH and NOS were localized in cell clusters between the segmental nerves. In G. lateralis, Gl-MIH and Gl-crustacean hyperglycemic hormone (CHH) mRNA levels were ~105-fold higher in ESG than in brain or TG of intermolt animals, indicating that the ESG is the primary source of these neuropeptides. Gl-NOS and Gl-elongation factor (EF2) mRNA levels were also higher in the ESG. Molt stage had little or no effect on CHH, NOS, NOS-interacting protein (NOS-IP), membrane Guanylyl Cyclase-II (GC-II), and NO-independent GC-III expression in the ESG of both species. By contrast, MIH and NO receptor GC-I beta subunit (GC-Iß) transcripts were increased during premolt and postmolt stages in G. lateralis, but not in C. maenas. MIH immunopositive cells in the brain and TG may be a secondary source of MIH; the release of MIH from these sources may contribute to the difference between the two species in response to ESA. The MIH-immunopositive cells in the TG may be the source of an MIH-like factor that mediates molt inhibition by limb bud autotomy. The association of MIH- and NOS-labeled cells in the ESG and TG suggests that NO may modulate MIH release. A model is proposed in which NO-dependent activation of GC-I inhibits Ca2+-dependent fusion of MIH vesicles with the nerve terminal membrane; the resulting decrease in MIH activates the YO and the animal enters premolt.
Assuntos
Proteínas de Artrópodes/metabolismo , Braquiúros/fisiologia , Sistema Nervoso Central/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Hormônios de Invertebrado/metabolismo , Neurônios/metabolismo , Óxido Nítrico Sintase/metabolismo , Animais , Aquicultura , Proteínas de Artrópodes/genética , Oceano Atlântico , Braquiúros/crescimento & desenvolvimento , California , Sistema Nervoso Central/citologia , Sistema Nervoso Central/enzimologia , República Dominicana , Olho , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Gânglios dos Invertebrados/metabolismo , Hormônios de Invertebrado/genética , Masculino , Muda , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Neurônios/citologia , Neurônios/enzimologia , Óxido Nítrico Sintase/genética , Córtex Olfatório/citologia , Córtex Olfatório/enzimologia , Córtex Olfatório/metabolismo , Especificidade de Órgãos , Oceano Pacífico , Especificidade da Espécie , TóraxRESUMO
Planorbid snails of the genus Biomphalaria are major intermediate hosts for the digenetic trematode parasite Schistosoma mansoni. Evidence suggests that levels of the neurotransmitter dopamine (DA) are reduced during the course of S. mansoni multiplication and transformation within the snail. This investigation used immunohistochemical methods to localize tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of catecholamines, in the nervous system of Biomphalaria. The two species examined, Biomphalaria glabrata and Biomphalaria alexandrina, are the major intermediate hosts for S. mansoni in sub-Saharan Africa, where more than 90% of global cases of human intestinal schistosomiasis occur. TH-like immunoreactive (THli) neurons were distributed throughout the central nervous system (CNS) and labeled fibers were present in all commissures, connectives, and nerves. Some asymmetries were observed, including a large distinctive neuron (LPeD1) in the pedal ganglion described previously in several pulmonates. The majority of TH-like immunoreactive neurons were detected in the peripheral nervous system (PNS), especially in lip and foot regions of the anterior integument. Independent observations supporting the dopaminergic phenotype of THli neurons included 1) block of LPeD1 synaptic signaling by the D2/3 antagonist sulpiride, and 2) the similar localization of aqueous aldehyde (FaGlu)-induced fluorescence. The distribution of THli neurons indicates that, as in other gastropods, dopamine functions as a sensory neurotransmitter and in the regulation of feeding and reproductive behaviors in Biomphalaria. It is hypothesized that infection could stimulate transmitter release from dopaminergic sensory neurons and that dopaminergic signaling could contribute to modifications of both host and parasite behavior.
Assuntos
Biomphalaria/enzimologia , Gânglios dos Invertebrados/enzimologia , Neurônios/enzimologia , Tirosina 3-Mono-Oxigenase/metabolismo , Animais , Biomphalaria/parasitologia , Biomphalaria/fisiologia , Catecolaminas/metabolismo , Sistema Nervoso Central/citologia , Sistema Nervoso Central/efeitos dos fármacos , Sistema Nervoso Central/enzimologia , Corantes , Dopamina/metabolismo , Antagonistas de Dopamina/farmacologia , Formaldeído , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/fisiologia , Glutaral , Imuno-Histoquímica , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Sistema Nervoso Periférico/citologia , Sistema Nervoso Periférico/enzimologia , Sistema Nervoso Periférico/fisiologia , Schistosoma mansoni , Especificidade da Espécie , Sulpirida/farmacologia , Transmissão Sináptica/efeitos dos fármacosRESUMO
Using NADPH-diaphorase (NADPH-d) histochemistry, inducible nitric oxide synthase (iNOS)-immunohistochemistry and immunoblotting, we characterized the nitric oxide (NO)-producing neurons in the brain and thoracic ganglion of a shore crab subjected to a nociceptive chemical stimulus. Formalin injection into the cheliped evoked specific nociceptive behavior and neurochemical responses in the brain and thoracic ganglion of experimental animals. Within 5-10 min of injury, the NADPH-d activity increased mainly in the neuropils of the olfactory lobes and the lateral antenna I neuropil on the side of injury. Later, the noxious-induced expression of NADPH-d and iNOS was detected in neurons of the brain, as well as in segmental motoneurons and interneurons of the thoracic ganglion. Western blotting analysis showed that an iNOS antiserum recognized a band at 120 kDa, in agreement with the expected molecular mass of the protein. The increase in nitrergic activity induced by nociceptive stimulation suggests that the NO signaling system may modulate nociceptive behavior in crabs.
Assuntos
Sistema Nervoso Central/metabolismo , Decápodes/metabolismo , Óxido Nítrico/metabolismo , Nociceptividade/fisiologia , Animais , Comportamento Animal/fisiologia , Western Blotting , Encéfalo/citologia , Encéfalo/enzimologia , Sistema Nervoso Central/citologia , Sistema Nervoso Central/enzimologia , Decápodes/citologia , Decápodes/enzimologia , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Masculino , NADPH Desidrogenase/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Estimulação Física , Fatores de TempoRESUMO
The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na(+)/K(+) pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na(+)/K(+)-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, (31)P NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na(+)/H(+) exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na(+)/K(+) pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na(+)/K(+) pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.
Assuntos
Encéfalo/enzimologia , Gânglios dos Invertebrados/enzimologia , Campos Magnéticos , Neurônios/enzimologia , Caramujos/enzimologia , ATPase Trocadora de Sódio-Potássio/metabolismo , Potenciais de Ação , Trifosfato de Adenosina/metabolismo , Animais , Encéfalo/citologia , Encéfalo/efeitos dos fármacos , GMP Cíclico/análogos & derivados , GMP Cíclico/farmacologia , Ativação Enzimática , Inibidores Enzimáticos/farmacologia , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética , Neurônios/efeitos dos fármacos , Ouabaína/farmacologia , Técnicas de Patch-Clamp , Periodicidade , Caramujos/citologia , Caramujos/efeitos dos fármacos , ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores , Fatores de TempoRESUMO
Using immunocytochemistry combined with light and electron microscopy, the distribution and ultrastructure of tyrosine hydroxylase (TH)-immunoreactive neurons in the CNS of bivalve mollusc, Megangulus venulosus, have been studied under the influence of increased temperature and hypoxia. It has been established, that the stress causes changes in the amount of TH and in the structure of TH-immunopositive neurons in all ganglia. The most essential changes in CNS of M. venulosus were revealed after 60 min exposure to increased temperature and hypoxia; degenerative changes in large neurons, reduction of the synapses and reduction of TH-immunoreactivity in neurons and neuropil.
Assuntos
Bivalves/ultraestrutura , Sistema Nervoso Central/fisiopatologia , Temperatura Alta , Hipóxia/fisiopatologia , Neurônios/ultraestrutura , Tirosina 3-Mono-Oxigenase/metabolismo , Animais , Bivalves/enzimologia , Bivalves/fisiologia , Sistema Nervoso Central/enzimologia , Sistema Nervoso Central/ultraestrutura , Gânglios dos Invertebrados/enzimologia , Gânglios dos Invertebrados/fisiopatologia , Gânglios dos Invertebrados/ultraestrutura , Hipóxia/enzimologia , Imuno-Histoquímica , Neurônios/enzimologiaRESUMO
In our study we investigated the influence of dopamine (DA) on the caudal photoreceptor (CPR) in crayfish. Here we report the following: (a) the chromatographic determination of DA in the sixth abdominal ganglion (6th AG) shows a variation in the content during a 24-h cycle with the maximum value at dawn. (b) There are possibly dopaminergic neurons in the 6th AG with antityrosine hydroxylase antibodies. Immunopositive neurons (164) were located in the anterior and posterior regions of the 6th AG with the mean (± SE) diameter of their somata 23 ± 1 µm. In addition, there is immunopositive staining in axons, neuropilar fibers, and varicosities. (c) We also identified, using immunohistochemistry, 108 neurons in the sixth AG that contain dopamine D1-like receptors, with the mean (±SE) diameter of their somata 18 ± 1 µm. (d) We examined the exogenous action of DA on the electrical activity of the CPR in the isolated sixth AG by conventional extracellular-recording methods. This CPR displays spontaneous activity and phasic-tonic responses to light pulses. Topical application of dopamine to ganglia kept in the dark increased the spontaneous firing rate of the CPR, whereas the photoresponse of the CPR remained unchanged. The effect on the spontaneous activity is dose-dependent with an ED50 of 33 µM, and is blocked by the dopamine D1-like antagonist SCH23390. These observations suggested that the DA is playing the role of a neurotransmitter or a neuromodulator of the CPR in the 6th AG in both species of crayfish, Procambarus clarkii and Cherax quadricarinatus.
Assuntos
Dopamina/fisiologia , Gânglios dos Invertebrados/metabolismo , Células Fotorreceptoras de Invertebrados/metabolismo , Animais , Astacoidea , Ritmo Circadiano/fisiologia , Feminino , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Masculino , Células Fotorreceptoras de Invertebrados/efeitos dos fármacos , Células Fotorreceptoras de Invertebrados/efeitos da radiação , Terminações Pré-Sinápticas/enzimologia , Terminações Pré-Sinápticas/metabolismo , Terminações Pré-Sinápticas/ultraestrutura , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
In this review a comparative analysis is performed of enzymological characteristics of cholinesterase (ChE) from optic ganglia of individuals of the Commander squid Berryteuthis magister caught in 8 zones of its habitation areal in the northern-western Pacific aquatorium, of ChE of the Pacific squid Todarodes pacificus as well as of the "standard" acetylcholinesterase from human erythrocytes and butyrylcholinesterase from horse blood serum. By the method of the substrate-inhibitor analysis there was shown heterogeneity of ChE preparations from the B. magister individuals from different habitation zones. Kinetic parameters of the enzymatic hydrolysis of 8 ester substrates are presented as well as the data on study of inhibitory specificity with use of 20 irreversible organophosphorus inhibitors, which show identity of ChE properties in the B. magister individuals from different habitation zones. Study of the process of the ChE reversible inhibition from the Commander squid individuals under action of 57 mono- and bisonium inhibitors has revealed differences in ChE properties of squid individuals from isolates in different zones of the habitation areal, which argues in favor of the existence of intraspecies groups of the Commander squid B. magister.
Assuntos
Colinesterases/química , Decapodiformes/enzimologia , Gânglios dos Invertebrados/enzimologia , Animais , Butirilcolinesterase/química , Colinesterases/metabolismo , Eritrócitos/enzimologia , Cavalos , Humanos , Hidrólise , Cinética , Especificidade da Espécie , Especificidade por SubstratoRESUMO
Neuronal nitric oxide (NO) levels are modulated through the control of catalytic activity of NO synthase (NOS). Although signals limiting excess NO synthesis are being extensively studied in the vertebrate nervous system, our knowledge is rather limited on the control of NOS in neurons of invertebrates. We have previously reported a transient inactivation of NOS in hibernating snails. In the present study, we aimed to understand the mechanism leading to blocked NO production during hypothermic periods of Helix pomatia. We have found that hypothermic challenge translocated NOS from the cytosol to the perinuclear endoplasmic reticulum, and that this cytosol to membrane trafficking was essential for inhibition of NO synthesis. Cold stress also downregulated NOS mRNA levels in snail neurons, although the amount of NOS protein remained unaffected in response to hypothermia. Our studies with cultured neurons and glia cells revealed that glia-neuron signaling may inhibit membrane binding and inactivation of NOS. We provide evidence that hypothermia keeps NO synthesis "hibernated" through subcellular redistribution of NOS.
Assuntos
Membrana Celular/metabolismo , Citosol/metabolismo , Caracois Helix/fisiologia , Hipotermia/metabolismo , Neurônios/citologia , Óxido Nítrico Sintase/metabolismo , Animais , Células Cultivadas , Resposta ao Choque Frio/fisiologia , Regulação para Baixo , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Gânglios dos Invertebrados/ultraestrutura , Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Neuroglia/citologia , Neuroglia/metabolismo , Neuroglia/ultraestrutura , Neurônios/metabolismo , Neurônios/ultraestrutura , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico Sintase/genética , Transporte Proteico/fisiologia , RNA Mensageiro/metabolismoRESUMO
Nitric oxide (NO) is a gas produced through the action of nitric oxide synthase that acts as a neurotransmitter in the central nervous system (CNS) of adult gastropod mollusks. There are no known reports of the presence of NOS-containing neurons and glial cells in young and adult Megalobulimus abbreviatus. Therefore, NADPH-d histochemistry was employed to map the nitrergic distribution in the CNS of young and adult snails in an attempt to identify any transient enzymatic activity in the developing CNS. Reaction was observed in neurons and fibers in all CNS ganglia of both age groups, but in the pedal and cerebral ganglia, positive neurons were more intense than in other ganglia, forming clusters symmetrically located in both paired ganglia. However, neuronal NADPH-d activity in the mesocerebrum and pleural ganglia decreased from young to adult animals. In both age groups, positive glial cells were located beneath the ganglionic capsule, forming a network and surrounding the neuronal somata. The trophospongium of large and giant neurons was only visualized in young animals. Our results indicate the presence of a nitrergic signaling system in young and adult M. abbreviatus, and the probable involvement of glial cells in NO production.
Assuntos
Sistema Nervoso Central/enzimologia , Gânglios dos Invertebrados/enzimologia , NADPH Desidrogenase/metabolismo , Óxido Nítrico Sintase/metabolismo , Envelhecimento , Animais , Sistema Nervoso Central/crescimento & desenvolvimento , CaramujosRESUMO
Comparative study of substrate specificity of monoamine oxidase (MAO) of optic ganglia of the Pacific squid Todarodes pacificus and the Commander squid Berryteuthis magister has been carried out. The enzyme of the Pacific squid, unlike that of the Commander squid, has been established to be able to deaminate not only tyramine, tryptamine, serotonin, benzylamine, and beta-phenylethylamine, but also histamine--substrate of diamine oxidase (DAO). In relation to all studied substrates, the MAO activity of optic ganglia of T. pacificus is several times higher as compared with B. magister. In the case of deamination of serotonin this difference was the greatest and amounted to 5 times. Semicarbazide, the classic DAO inhibitor, at a concentration of 10 mM did not inhibit catalytic activity of both studied enzymes. The substrate-inhibitory analysis with use of deprenyl and chlorogiline, specific inhibitors of different MAO forms, indicates homogeneity of the enzyme of the Pacific squid and heterogeneity of the Commander squid enzyme whose composition seems probably to contain at least two MAO forms. There are obtained quantitative differences in substrate specificity and reaction capability with respect to the inhibitors chlorgiline and deprenyl for MAO of optic ganglia of the studied squid species. These differences probably can be explained by significant differences in the evolutionary level of these biological species.
Assuntos
Decapodiformes/enzimologia , Olho/enzimologia , Gânglios dos Invertebrados/enzimologia , Monoaminoxidase/metabolismo , Animais , Olho/inervação , Inibidores da Monoaminoxidase/farmacologia , Especificidade da Espécie , Especificidade por SubstratoRESUMO
Nitric oxide (NO) is an evolutionarily conserved mediator of neural plasticity. Because NO is highly diffusible, signals from multiple sources might combine in space and time to affect the same target. Whether such cooperative effects occur will depend on the effective signaling range and on the distances of NO sources to one another and to their targets. These anatomical parameters have been quantified in only few systems. We analyzed the 3D architecture of NO synthase (NOS) expression in a sensory neuropil, the ventral association center (VAC) of the locust. High-resolution confocal microscopy revealed NOS immunoreactive fiber boutons in submicrometer proximity to both the axon terminals of sensory neurons and their postsynaptic target, interneuron A4I1. Pharmacological manipulation of NO signaling affected the response of A4I1 to individual wind-puff stimuli and the response decrement during repetitive stimulation. Mapping NOS immunoreactivity in defined volumes around dendrites of A4I1 revealed NOS-positive fiber boutons within 5 mum of nearly every surface point. The mean distances between neighboring NOS-boutons and between any point within the VAC and its nearest NOS-bouton were likewise about 5 mum. For an NO signal to convey the identity of its source, the effective signaling range would therefore have to be less than 5 mum, and shorter still when multiple boutons release NO simultaneously. The architecture is therefore well suited to support the cooperative generation of volume signals by interaction between the signals from multiple active boutons.
Assuntos
Locusta migratoria/fisiologia , Mecanorreceptores/metabolismo , Óxido Nítrico/metabolismo , Animais , Contagem de Células , Dendritos/enzimologia , Dendritos/metabolismo , Feminino , Gânglios dos Invertebrados/enzimologia , Gânglios dos Invertebrados/metabolismo , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Masculino , Microscopia Confocal , Rede Nervosa/metabolismo , Rede Nervosa/fisiologia , Óxido Nítrico Sintase Tipo I/metabolismo , Terminações Pré-Sinápticas/enzimologia , Terminações Pré-Sinápticas/metabolismo , Transdução de Sinais/fisiologia , Fixação de TecidosRESUMO
The MAPK/ERK pathway plays an important role in the regulation of gene expression during memory formation both in vertebrates and invertebrates. In the mollusk Helix lucorum, serotonin induces activation of MAPK/ERK in the central nervous system (CNS) upon food aversion learning. Such learning depends on a neuronal network in which specialized neurons play distinct roles so that they may exhibit different activation levels of the MAPK/ERK pathway. Here we performed a comparative analysis of MAPK/ERK activation in single neurons of the food-aversion network, focusing both on command neurons, which mediate withdrawal behavior and process information pertaining to the unconditioned stimulus, and on neurons of the procerebrum, the mollusk's olfactory center, which process information from the conditioned stimulus. By means of Western blots designed to detect micro amounts of proteins, we determined MAPK/ERK activation in these neurons and found that after food aversion learning phospho-ERK levels increased significantly in RPa(2/3) command neurons of the right parietal ganglia and in the procerebrum. Such an increase was prevented by injection of PD98095, an inhibitor of the ERK upstream kinase (MEK-1). In contrast, no activation of MAPK/ERK was detected in similar conditions in the corresponding neurons of the left parietal ganglia LPa(2/3). This asymmetry was verified after serotonin application to the CNS in order to mimic learning. Our results thus show that learning involves synchronous and asymmetric serotonin-dependent MAPK/ERK activation. Such an asymmetry may reflect lateralization of memory processes in the mollusk brain.
Assuntos
Aprendizagem da Esquiva/fisiologia , Lateralidade Funcional/fisiologia , Caracois Helix/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Neurônios/enzimologia , Análise de Variância , Animais , Sequência de Bases , Condicionamento Clássico/fisiologia , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Dados de Sequência Molecular , Rede Nervosa/citologia , Rede Nervosa/enzimologia , Sistemas do Segundo Mensageiro/fisiologia , Homologia de Sequência do Ácido Nucleico , Transdução de Sinais/fisiologia , Estatísticas não ParamétricasRESUMO
Summarized are results of the 40-year studies of the Russian biochemists on the comparative-enzymological characteristics of cholinesterase of optic ganglia of the Pacific squid Todarodes pacificus. The review includes the comparative evaluation of the cholinesterase activity of various hydrobiont tissues, the proof of enzymatic homogeneity of the tissue of the Pacific squid optic ganglia, data on substrate specificity with study of 18 ester substrates as well as detailed study of inhibitory specificity (61 irreversible inhibitors and 49 reversible onium inhibitors). Peculiarity of properties of this enzyme as compared with vertebrate and invertebrate cholinesterases is shown.
Assuntos
Colinesterases/química , Colinesterases/metabolismo , Decapodiformes/enzimologia , Gânglios dos Invertebrados/enzimologia , Animais , Especificidade por Substrato , VertebradosRESUMO
Expression, localization and regulation of different cAMP-dependent protein kinase A (PKA) subunits account for specificity in the intracellular cAMP/PKA signaling pathway. In Aplysia neurons, two classes of PKA (I and II) differing in their regulatory (R) subunits have been characterized. Type I is mostly soluble in the cell body, and type II enriched at the synaptic endings. Although both types are necessary for long-term changes in synaptic plasticity, their differences in cellular localization and expression suggest that they mediate distinct functions. By photoaffinity labeling studies, we previously observed a cAMP-binding 105 kDa band in extracts from Aplysia neurons as a putative third class of R subunit of PKA. Here, we have determined that the 105 kDa band is a high molecular weight complex (HMWC) containing alpha/beta-tubulin and PKA RI, but not RII. This hetero-complex is conserved in vertebrates since mouse brain extracts also contain it. The enrichment of the endogenous HMWC by subcellular fractionation and its synthesis in vitro indicate that it is mainly produced in the cytosol, and then transported to the synapses. The HMWC is functional as a cAMP-sensitive regulatory subunit of PKA since it binds catalytic subunit in the absence of cAMP. Furthermore, serotonin (5-HT) treatment, which produces long-term facilitation in neurons, induced its degradation. In mouse brain RI co-localized with tubulin in neuropils and in COS-7 cells discretely at the cell membrane. These observations suggest that the alpha/beta-tubulin anchoring type I PKA may have an important role in the formation of long-term synaptic plasticity.
Assuntos
Proteínas de Ancoragem à Quinase A/metabolismo , Aplysia/enzimologia , Proteína Quinase Tipo I Dependente de AMP Cíclico/metabolismo , Gânglios dos Invertebrados/enzimologia , Neurônios/enzimologia , Tubulina (Proteína)/metabolismo , Animais , Aplysia/citologia , Células COS , Sistema Nervoso Central/citologia , Sistema Nervoso Central/enzimologia , Chlorocebus aethiops , Subunidade RIIalfa da Proteína Quinase Dependente de AMP Cíclico/metabolismo , Subunidade RIalfa da Proteína Quinase Dependente de AMP Cíclico/metabolismo , Gânglios dos Invertebrados/citologia , Substâncias Macromoleculares/química , Substâncias Macromoleculares/metabolismo , Camundongos , Peso Molecular , Plasticidade Neuronal/fisiologia , Roedores/metabolismo , Especificidade da Espécie , Frações SubcelularesRESUMO
The cAMP-dependent protein kinase (PKA) is known to play a critical role in both transcription-independent short-term or intermediate-term memory and transcription-dependent long-term memory (LTM). Although distinct phases of LTM already have been demonstrated in some systems, it is not known whether these phases require distinct temporal patterns of learning-induced PKA activation. This question was addressed in a robust form of associative LTM that emerges within a matter of hours after single-trial food-reward classical conditioning in the pond snail Lymnaea stagnalis. After establishing the molecular and functional identity of the PKA catalytic subunit in the Lymnaea nervous system, we used a combination of PKA activity measurement and inhibition techniques to investigate its role in LTM in intact animals. PKA activity in ganglia involved in single-trial learning showed a short latency but prolonged increase after classical conditioning. However, while increased PKA activity immediately after training (0-10 min) was essential for an early phase of LTM (6 h), the late phase of LTM (24 h) required a prolonged increase in PKA activity. These observations indicate mechanistically different roles for PKA in recent and more remote phases of LTM, which may underpin different cellular and molecular mechanisms required for these phases.
Assuntos
Encéfalo/enzimologia , Condicionamento Clássico/fisiologia , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Lymnaea/fisiologia , Memória/fisiologia , Animais , Western Blotting , Proteínas Quinases Dependentes de AMP Cíclico/genética , Gânglios dos Invertebrados/enzimologia , Imuno-Histoquímica , Hibridização In Situ , FilogeniaRESUMO
NADPH-diaphorase (NADPH-d) is a histochemical marker for nitric oxide synthase (NOS) and is widely used to identify nitric oxide (NO) producing cells in the central nervous system (CNS) of both vertebrates and invertebrates. NADPH-d histochemistry was used to quantitatively characterize putative NO-producing neurons in the CNS of the Gray mussel Crenomytilus grayanus subjected to two kinds of stress, environmental pollution and hypoxia, the latter caused by the mollusk transportation in a small volume of water. Mussels were sampled from one relatively clean (reference) and four polluted sites in Amursky and Ussuriysky Bays (Peter the Great Bay, Sea of Japan) in August, 2003. The number of NADPH-d-positive neurons was estimated and enzyme activity was determined from the optical density of the formazan precipitate in the CNS ganglia at 0, 3, and 72 h after sampling. Just after sampling, NADPH-d-positive neurons were found in the cerebropleural, visceral, and pedal ganglia. The number and staining intensity of NADPH-d-positive neurons were significantly higher in the pedal ganglia than the other two ganglia. There were significant differences in the number of NADPH-d-positive neurons and enzyme activity between the mussels from the reference and heavily polluted stations. The proportion and staining intensity of NADPH-d-positive neurons were maximum in the pedal ganglia of the mussels from the heavily polluted station in Amursky Bay. Transportation of mussels in a limited volume of water for 3h resulted in a significant increase in the proportion and staining intensity of NADPH-d-positive neurons in all ganglia. In mollusks from all stations kept in aerated aquaria for 72 h, both the proportion and staining intensity of NADPH-d-positive neurons did not differ significantly from the initial level. However, the differences in the proportion and staining intensity of NADPH-d-positive neurons between the reference and heavily polluted stations were significant. The present results suggest that NO is involved in mollusk nerve cell adaptation to environmental changes.
Assuntos
Bivalves/enzimologia , Sistema Nervoso Central/enzimologia , Poluição Ambiental/efeitos adversos , NADPH Desidrogenase/metabolismo , Óxido Nítrico/metabolismo , Adaptação Fisiológica , Animais , Bivalves/metabolismo , Sistema Nervoso Central/citologia , Monitoramento Ambiental/métodos , Gânglios dos Invertebrados/citologia , Gânglios dos Invertebrados/enzimologia , Histocitoquímica , Hipóxia , Neurônios/enzimologia , Óxido Nítrico Sintase/análise , Óxido Nítrico Sintase/metabolismoRESUMO
Calmodulin and Abelson tyrosine kinase are key signaling molecules transducing guidance cues at the Drosophila embryonic midline. A reduction in the signaling strength of either pathway alone induces ectopic midline crossing errors in a few segments. When Calmodulin and Abelson signaling levels are simultaneously reduced, the frequency of ectopic crossovers is synergistically enhanced as all segments exhibit crossing errors. But as the level of signaling is further reduced, commissures begin to fuse and large gaps form in the longitudinal connectives. Quantitative analysis suggests that the level of Abelson activity is particularly important. Like Calmodulin, Abelson interacts with son-of-sevenless to increase ectopic crossovers suggesting all three contribute to midline repulsive signaling. Axons cross the midline in almost every segment if Frazzled is co-overexpressed with the Calmodulin inhibitor, but the crossovers induced by the Calmodulin inhibitor itself do not require endogenous Frazzled. Thus, Calmodulin and Abelson tyrosine kinase are key signaling molecules working synergistically to transduce both midline attractive and repulsive cues. While they may function downstream of specific receptors, the emergence of commissural and longitudinal connective defects point to a novel convergence of Calmodulin and Abelson signaling during the regulation of actin and myosin dynamics underlying a guidance decision.
Assuntos
Padronização Corporal/genética , Calmodulina/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/embriologia , Cones de Crescimento/enzimologia , Sistema Nervoso/embriologia , Proteínas Tirosina Quinases/metabolismo , Citoesqueleto de Actina/metabolismo , Animais , Calmodulina/genética , Sinais (Psicologia) , Proteínas de Drosophila/genética , Drosophila melanogaster/enzimologia , Embrião não Mamífero/embriologia , Embrião não Mamífero/enzimologia , Desenvolvimento Embrionário/genética , Lateralidade Funcional/fisiologia , Gânglios dos Invertebrados/embriologia , Gânglios dos Invertebrados/enzimologia , Regulação da Expressão Gênica no Desenvolvimento/genética , Cones de Crescimento/ultraestrutura , Miosinas/metabolismo , Sistema Nervoso/enzimologia , Receptores de Netrina , Proteínas Tirosina Quinases/genética , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Transdução de Sinais/genética , Proteína Son Of Sevenless de Drosófila/genética , Proteína Son Of Sevenless de Drosófila/metabolismoRESUMO
Target-dependent increases in axon growth and varicosities accompany the formation of functional synapses between Aplysia sensory neurons and specific postsynaptic neurons (L7 and not L11). The enhanced growth is regulated in part by a target-dependent increase in the secretion of sensorin, the sensory neuron neuropeptide. We report here that protein kinase C (PKC) activity is required for synapse formation by sensory neurons with L7 and for the target-dependent increases in sensorin synthesis and secretion. Blocking PKC activity reversibly blocked synapse formation and axon growth of sensory neurons contacting L7, but did not affect axon growth of sensory neurons contacting L11 or axon growth of the postsynaptic targets. Blocking PKC activity also blocked the target-induced increase in sensorin synthesis and secretion. Sensorin then activates additional signaling pathways required for synapse maturation and synapse-associated growth. Exogenous anti-sensorin antibody blocked target-induced activation and translocation into sensory neuron nuclei of p42/44 mitogen-activated protein kinase (MAPK), attenuated synapse maturation, and curtailed growth of sensory neurons contacting L7, but not the growth of sensory neurons contacting L11. Inhibitors of MAPK or phosphoinositide 3-kinase also attenuated synapse maturation and curtailed growth and varicosity formation of sensory neurons contacting L7, but not growth of sensory neurons contacting L11. These results suggest that PKC activity regulated by specific cell-cell interactions initiates the formation of specific synapses and the subsequent synthesis and release of a neuropeptide to activate additional signaling pathways required for synapse maturation.
Assuntos
Proteína Quinase C/fisiologia , Sinapses/enzimologia , Animais , Aplysia , Células Cultivadas , Inibidores Enzimáticos/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Gânglios dos Invertebrados/efeitos dos fármacos , Gânglios dos Invertebrados/enzimologia , Gânglios dos Invertebrados/crescimento & desenvolvimento , Proteína Quinase C/antagonistas & inibidores , Sinapses/efeitos dos fármacosRESUMO
Western blot analysis was used to study the activation of MAP/ERK protein kinases responsible for controlling gene expression via phosphorylation of transcription factors CREB and ELK-1 in native common snails and animals with impaired abilities to form long-term types of conditioned aversive reflexes. Different periods of the formation of this reflex were found to be characterized by different levels of activation of MAP-ERK kinases. The extents of activation of MAP-ERK kinase cascade were different in ganglia (parietal-visceral, cerebral, and pedal) with different roles in the formation of this reflex. The dynamics of activation showed a wavelike nature, with peaks at 10 min and 4 h. Administration of the neurotoxin 5,7-DHT, which induces dysfunction of serotonin terminals and decreases the ability to acquire this type of learning, led to significant decreases in activation of the MAP-ERK kinase cascade at the early stages of learning, which is evidence for an important role for the serotoninergic system in inducing this cascade. Activation of the MAP/ERK kinase cascade 4 h after training was seen both in native and DHT-treated animals, which is probably evidence for activation of non-specific adaptive processes in response to the sensitizing unconditioned stimulus. Thus, the MAP/ERK kinase intracellular regulatory cascade, which plays an important role in the survival of neurons, the regeneration of neuron processes, and synaptic sprouting, also plays an important role in forming the serotonin-dependent food-aversive reflex in the common snail.