RESUMO
The dihydroneopterin aldolase (DHNA, EC 4.1.2.25) activity of FolB protein is required for the conversion of 7,8-dihydroneopterin (DHNP) to 6-hydroxymethyl-7,8-dihydropterin (HP) and glycolaldehyde (GA) in the folate pathway. FolB protein from Mycobacterium tuberculosis (MtFolB) is essential for bacilli survival and represents an important molecular target for drug development. S8-functionalized 8-mercaptoguanine derivatives were synthesised and evaluated for inhibitory activity against MtFolB. The compounds showed IC50 values in the submicromolar range. The inhibition mode and inhibition constants were determined for compounds that exhibited the strongest inhibition. Additionally, molecular docking analyses were performed to suggest enzyme-inhibitor interactions and ligand conformations. To the best of our knowledge, this study describes the first class of MtFolB inhibitors.
Assuntos
Aldeído Liases/antagonistas & inibidores , Antibacterianos/farmacologia , Inibidores Enzimáticos/farmacologia , Guanosina/análogos & derivados , Simulação de Acoplamento Molecular , Mycobacterium tuberculosis/efeitos dos fármacos , Tionucleosídeos/farmacologia , Aldeído Liases/genética , Aldeído Liases/metabolismo , Antibacterianos/síntese química , Antibacterianos/química , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Guanosina/síntese química , Guanosina/química , Guanosina/farmacologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Mycobacterium tuberculosis/enzimologia , Tionucleosídeos/síntese química , Tionucleosídeos/químicaRESUMO
Among the non-canonical structures of B-DNA, the G-quadruplex is of particular interest because of its well-defined conformation, high stability, and versatility. Herein we report our studies on the development of an amide-linked minimal diguanosinyl motif that forms a G-quadruplex-like structure in solution in the presence of potassium cations; various linear guanosine amino acid dimers were synthesized with linkers of different chain lengths to investigate the optimum flexibility required to form such structures.
Assuntos
Quadruplex G , Guanosina/química , RNA/química , Dicroísmo Circular , Dimerização , Guanosina/síntese química , Conformação de Ácido Nucleico , Soluções/químicaRESUMO
A multifunctional G-PyB/KCl hydrogel showed outstanding self-healability, high conductivity, and rapid room-temperature phase-selective gelation capacity, and was developed as an electrochemical aptamer sensing platform for the ultrasensitive detection of alpha-fetoprotein.
Assuntos
Ácidos Borônicos/química , Guanosina/análogos & derivados , Hidrogéis/química , Cloreto de Potássio/química , Piridinas/química , alfa-Fetoproteínas/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Ouro/química , Guanosina/síntese química , Hidrogéis/síntese química , Limite de Detecção , Transição de Fase , Porosidade , Rodaminas/química , Tolueno/química , Temperatura de TransiçãoRESUMO
Eukaryotic translation initiation factor 4E (eIF4E) is a key player in the initiation of cap-dependent translation through recognition of the m7GpppX cap at the 5' terminus of coding mRNAs. As eIF4E overexpression has been observed in a number of human diseases, most notably cancer, targeting this oncogenic translation initiation factor has emerged as a promising strategy for the development of novel anti-cancer therapeutics. Toward this end, in the present study, we have rationally designed a series of Bn7GxP-based PROTACs for the targeted degradation of eIF4E. Herein we describe our synthetic efforts, in addition to biochemical and cellular characterization of these compounds.
Assuntos
Desenho de Fármacos , Fator de Iniciação 4E em Eucariotos/metabolismo , Guanosina/análogos & derivados , Proteólise/efeitos dos fármacos , Linhagem Celular Tumoral , Técnicas de Química Sintética , Fator de Iniciação 4E em Eucariotos/química , Guanosina/síntese química , Guanosina/química , Guanosina/farmacologia , Humanos , Modelos Moleculares , Conformação ProteicaRESUMO
6-Thioguanine (1a) is considered to be photochemotherapeutic due to its specific characteristics of photosensitivity to UVA light and singlet molecular oxygen generation. To extend its phototherapeutic ability, two related thioguanines, 8-thioguanine (2a) and 6,8-dithioguanine (3a), have been designed and explored. Since the solubility of these thioguanines in dehydrated organic solvents is too poor to study, their triacetyl-protected ribonucleosides, that is, 2',3',5'-tri-O-acetyl-6-thioguanosine (1c), 2',3',5'-tri-O-acetyl-8-thioguanosine (2c) and 2',3',5'-tri-O-acetyl-6,8-dithioguanosine (3c) were prepared and investigated. The absorption maxima of 1c, 2c and 3c in acetonitrile were found at longer wavelengths than that of unthiolated guanosine (4c). Especially, 3c has the longest wavelength for absorption maximum and the highest value in terms of molar absorption coefficient among all thionucleobases and thionucleosides reported. These absorption properties were also well reproduced by quantum chemical calculations. Quantum yields of singlet oxygen generation of 2c and 3c were determined by near-infrared emission measurements to be as large as that of 1c. These results suggest that the newly synthesized thioguanosines, in particular 3c, can be further developed as a potential photosensitive agent for light-induced therapies.
Assuntos
Guanosina/análogos & derivados , Teoria Quântica , Oxigênio Singlete/química , Tionucleosídeos/química , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Guanosina/síntese química , Guanosina/química , Processos Fotoquímicos , Fotoquimioterapia , Fármacos Fotossensibilizantes/síntese química , Espectrofotometria Ultravioleta , Espectroscopia de Luz Próxima ao Infravermelho , Tionucleosídeos/síntese químicaRESUMO
A variety of applications of 8-alkynylated nucleosides has prompted the synthesis of new purine analogues. Bromination of unprotected 2-amino-2'-deoxyadenosine with Br2 /AcOH/AcONa gives 2-amino-8-bromo-2'-deoxyadenosine (87%). The brominated derivative is converted to 8-alkynylated 2-amino-2'-deoxyadenosines by palladium-catalyzed Sonogashira cross-coupling reaction via microwave assistance (81 - 95%). The resulting compounds are further transformed to 8-alkynylated 2'-deoxyisoguanosines (52 - 70%). The physical properties of new compounds are investigated.
Assuntos
Guanosina/síntese química , Adenosina , Guanosina/química , Halogenação , Concentração de Íons de Hidrogênio , Micro-Ondas , Conformação MolecularRESUMO
We report a kinetic and mechanistic study on the title reactions, in which 1O2 was generated by the reaction of H2O2 with Cl2 and bubbled into an aqueous solution of guanine and 9-methylguanine (9MG) at different pH values. Oxidation kinetics and product branching ratios were measured using online electrospray ionization mass spectrometry coupled with absorption and emission spectrophotometry, and product structures were determined by collision-induced dissociation (CID) tandem mass spectrometry. Experiments revealed strong pH dependence of the reactions. The oxidation of guanine is noticeable only in basic solution, while the oxidation of 9MG is weak in acidic solution, increases in neutral solution, and becomes intensive in basic solution. 5-Guanidinohydantoin (Gh) and spiroiminodihydantoin (Sp) were detected as the major oxidation products of guanine and 9MG, and Sp became dominant in basic solution. A reaction intermediate was captured in mass spectra, and assigned to gem-diol on the basis of CID measurements. This intermediate served as the precursor for the formation of Gh. After taking into account solution compositions at each pH, first-order oxidation rate constants were extracted for individual species: that is, 3.2-3.6 × 107 M-1 s-1 for deprotonated guanine, and 1.2 × 106 and 4.6-4.9 × 107 M-1 s-1 for neutral and deprotonated 9MG, respectively. Guided by approximately spin-projected density-functional-theory-calculated reaction potential energy surfaces, the kinetics for the initial 1O2 addition to guanine and 9MG was evaluated using transition state theory (TST). The comparison between TST modeling and experiment confirms that 1O2 addition is rate-limiting for oxidation, which forms endoperoxide and peroxide intermediates as determined in previous measurements of the same systems in the gas phase.
Assuntos
Guanina/análogos & derivados , Guanina/química , Oxigênio Singlete/química , Cloro/química , Guanidinas/síntese química , Guanosina/análogos & derivados , Guanosina/síntese química , Hidantoínas/síntese química , Peróxido de Hidrogênio/química , Concentração de Íons de Hidrogênio , Cinética , Modelos Químicos , Conformação Molecular , Oxirredução , Espectrometria de Massas por Ionização por Electrospray , Compostos de Espiro/síntese química , Espectrometria de Massas em TandemRESUMO
6-O-(2-Nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates (NBGTP, NBUTP) were synthesized, and their biochemical and photophysical properties were evaluated. We synthesized NBUTP using the canonical triphosphate synthesis method and NBGTP from 2',3'-O-TBDMS guanosine via a triphosphate synthesis method by utilizing mild acidic desilylation conditions. Deprotection of the nitrobenzyl group in NBGTP and NBUTP proceeded within 60s by UV irradiation at 365nm. Experiments using NBGTP or NBUTP in T7-RNA transcription reactions showed that NBGTP could be useful for the photocontrol of transcription by UV irradiation.
Assuntos
RNA Polimerases Dirigidas por DNA/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Guanosina/farmacologia , Transcrição Gênica/efeitos dos fármacos , Raios Ultravioleta , Uridina Trifosfato/farmacologia , Proteínas Virais/antagonistas & inibidores , RNA Polimerases Dirigidas por DNA/genética , RNA Polimerases Dirigidas por DNA/metabolismo , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Guanosina/análogos & derivados , Guanosina/síntese química , Estrutura Molecular , Relação Estrutura-Atividade , Transcrição Gênica/genética , Uridina Trifosfato/síntese química , Uridina Trifosfato/química , Proteínas Virais/genética , Proteínas Virais/metabolismoRESUMO
An amphiphilic derivative of guanosine, carrying a myristoyl group at the 5'-position and two methoxy(triethylene glycol) appendages at the 2' and 3'-positions (1), endowed with high ionophoric activity, has been here studied in its interaction mode with a model lipid membrane along with its 5'-spin-labelled analogue 2, bearing the 5-doxyl-stearic in lieu of the myristic residue. Electron spin resonance spectra, carried out on the spin-labelled nucleolipid 2 in mixture with a DOPC/DOPG phospholipid bilayer, on one side, and on spin-labelled lipids mixed with 1, on the other, integrated with dynamic light scattering and neutron reflectivity measurements, allowed getting an in-depth picture of the effect of the ionophores on membrane structure, relevant to clarify the ion transport mechanism through lipid bilayers. Particularly, dehydration of lipid headgroups and lowering of both the local polarity and acyl chains order across the bilayer, due to the insertion of the oligo(ethylene glycol) chains in the bilayer hydrophobic core, have been found to be the main effects of the amphiphilic guanosines interaction with the membrane. These results furnish directions to rationally implement future ionophores design.
Assuntos
Guanosina/análogos & derivados , Ionóforos/química , Bicamadas Lipídicas/química , Fosfatidilcolinas/química , Fosfatidilgliceróis/química , Desenho de Fármacos , Espectroscopia de Ressonância de Spin Eletrônica , Guanosina/síntese química , Interações Hidrofóbicas e Hidrofílicas , Ionóforos/síntese química , Cinética , Luz , Polietilenoglicóis/química , Espalhamento de Radiação , Marcadores de SpinRESUMO
The most common, oxidatively generated lesion in cellular DNA is 8-oxo-7,8-dihydroguanine, which can be oxidized further to yield highly mutagenic spiroiminodihydantoin (Sp) and 5-guanidinohydantoin (Gh) in DNA. In human cell-free extracts, both lesions can be excised by base excision repair and global genomic nucleotide excision repair. However, it is not known if these lesions can be removed by transcription-coupled DNA repair (TCR), a pathway that clears lesions from DNA that impede RNA synthesis. To determine if Sp or Gh impedes transcription, which could make each a viable substrate for TCR, either an Sp or a Gh lesion was positioned on the transcribed strand of DNA under the control of a promoter that supports transcription by human RNA polymerase II. These constructs were incubated in HeLa nuclear extracts that contained active RNA polymerase II, and the resulting transcripts were resolved by denaturing polyacrylamide gel electrophoresis. The structurally rigid Sp strongly blocks transcription elongation, permitting 1.6 ± 0.5% nominal lesion bypass. In contrast, the conformationally flexible Gh poses less of a block to human RNAPII, allowing 9 ± 2% bypass. Furthermore, fractional lesion bypass for Sp and Gh is minimally affected by glycosylase activity found in the HeLa nuclear extract. These data specifically suggest that both Sp and Gh may well be susceptible to TCR because each poses a significant block to human RNA polymerase II progression. A more general principle is also proposed: Conformational flexibility may be an important structural feature of DNA lesions that enhances their transcriptional bypass.
Assuntos
Guanidinas/farmacologia , Guanosina/análogos & derivados , Hidantoínas/farmacologia , RNA Polimerase II/antagonistas & inibidores , Compostos de Espiro/farmacologia , Elongação da Transcrição Genética/efeitos dos fármacos , Dano ao DNA , Reparo do DNA , Guanidinas/síntese química , Guanidinas/química , Guanosina/síntese química , Guanosina/química , Guanosina/farmacologia , Células HeLa , Humanos , Hidantoínas/síntese química , Hidantoínas/química , Conformação Molecular , RNA Polimerase II/genética , RNA Polimerase II/metabolismo , Compostos de Espiro/síntese química , Compostos de Espiro/química , Relação Estrutura-AtividadeRESUMO
Phosphorylation and dephosphorylation of splicing factors play a key role in pre-mRNA splicing events, and cantharidin and norcantharidin analogs inhibit protein phosphatase-1 (PP1) and change alternative pre-mRNA splicing. Targeted inhibitors capable of selectively inhibiting PP-1 could promote exon 7 inclusion in the survival-of-motorneuron-2 gene (SMN2) and shift the proportion of SMN2 protein from a dysfunctional to a functional form. As a prelude to the development of norcantharidin-tethered oligonucleotide inhibitors, the synthesis a norcantharidin-tethered guanosine was developed in which a suitable tether prevented the undesired cyclization of norcantharidin monoamides to imides and possessed a secondary amine terminus suited to the synthesis of oligonucleotides analogs. Application of this methodology led to the synthesis of a diastereomeric mixture of norcantharidin-tethered guanosines, namely bisammonium (1R,2S,3R,4S)- and (1S,2R,3S,4R)-3-((4-(2-(((((2R,3R,4R,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-2-(hydroxymethyl)-4-methoxytetrahydrofuran-3-yl)oxy)oxidophosphoryl)oxy)ethyl)-phenethyl)(methyl)carbamoyl)-7-oxabicyclo[2.2.1]heptane-2-carboxylate, which showed activity in an assay for SMN2 pre-mRNA splicing.
Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes/química , Inibidores Enzimáticos/síntese química , Guanosina/análogos & derivados , Proteína Fosfatase 1/antagonistas & inibidores , Processamento Alternativo , Animais , Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Guanosina/síntese química , Guanosina/metabolismo , Células HEK293 , Humanos , Camundongos , Proteína Fosfatase 1/metabolismo , RNA Mensageiro/metabolismo , Proteína 2 de Sobrevivência do Neurônio Motor/genética , Proteína 2 de Sobrevivência do Neurônio Motor/metabolismoRESUMO
The guanine base in DNA, due to its low oxidation potential, is particularly sensitive to chemical modifications. A large number of guanine lesions have been characterized and studied in some detail due to their relationship with tissue inflammations. Nevertheless, one example of these lesions is the formation of 8-nitro-guanosine, but the NMR data of this compound was only partially interpreted. A comprehensive study of the two possible tautomeric forms, through a detailed characterization of this compound, has implications for its base pairing properties. The target compound was obtained through a synthetic sequence of five steps, where all intermediates were fully characterized using spectral data. The analysis of the two tautomers was then evaluated through NMR spectroscopy and theoretical calculations of the chemical shifts and NH coupling constants, which were also compared with the data from guanosine.
Assuntos
Guanosina/análogos & derivados , Espectroscopia de Ressonância Magnética , Modelos Teóricos , Nitrocompostos/química , DNA/química , Guanosina/síntese química , Guanosina/química , Hidrólise , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Nitrocompostos/síntese químicaRESUMO
Cladribine, 2-chloro-2'-deoxyadenosine, is a highly efficacious, clinically used nucleoside for the treatment of hairy cell leukemia. It is also being evaluated against other lymphoid malignancies and has been a molecule of interest for well over half a century. In continuation of our interest in the amide bond-activation in purine nucleosides via the use of (benzotriazol-1yl-oxy)tris(dimethylamino)phosphonium hexafluorophosphate, we have evaluated the use of O6-(benzotriazol-1-yl)-2'-deoxyguanosine as a potential precursor to cladribine and its analogues. These compounds, after appropriate deprotection, were assessed for their biological activities, and the data are presented herein. Against hairy cell leukemia (HCL), T-cell lymphoma (TCL) and chronic lymphocytic leukemia (CLL), cladribine was the most active against all. The bromo analogue of cladribine showed comparable activity to the ribose analogue of cladribine against HCL, but was more active against TCL and CLL. The bromo ribose analogue of cladribine showed activity, but was the least active among the C6-NH2-containing compounds. Substitution with alkyl groups at the exocyclic amino group appears detrimental to activity, and only the C6 piperidinyl cladribine analogue demonstrated any activity. Against adenocarcinoma MDA-MB-231 cells, cladribine and its ribose analogue were most active.
Assuntos
Antineoplásicos/síntese química , Cladribina/síntese química , Guanosina/síntese química , Leucócitos Mononucleares/efeitos dos fármacos , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Cladribina/farmacologia , Guanosina/farmacologia , Humanos , Concentração Inibidora 50 , Leucemia de Células Pilosas/patologia , Leucemia Linfocítica Crônica de Células B/patologia , Leucócitos Mononucleares/patologia , Linfoma de Células T/patologia , Compostos Organofosforados/química , Cultura Primária de Células , Relação Estrutura-AtividadeRESUMO
Although numerous reports on the synthesis of atom-specific (15)N-labeled nucleosides exist, fast and facile access to the corresponding phosphoramidites for RNA solid-phase synthesis is still lacking. This situation represents a severe bottleneck for NMR spectroscopic investigations on functional RNAs. Here, we present optimized procedures to speed up the synthesis of (15)N(1) adenosine and (15)N(1) guanosine amidites, which are the much needed counterparts of the more straightforward-to-achieve (15)N(3) uridine and (15)N(3) cytidine amidites in order to tap full potential of (1)H/(15)N/(15)N-COSY experiments for directly monitoring individual Watson-Crick base pairs in RNA. Demonstrated for two preQ1 riboswitch systems, we exemplify a versatile concept for individual base-pair labeling in the analysis of conformationally flexible RNAs when competing structures and conformational dynamics are encountered.
Assuntos
Adenosina/síntese química , Citidina/química , Guanosina/síntese química , Nucleosídeos/química , Fosforamidas/química , Fosforamidas/síntese química , RNA/química , Uridina/química , Adenosina/química , Pareamento de Bases , Guanosina/química , Espectroscopia de Ressonância Magnética , Conformação de Ácido Nucleico , Técnicas de Síntese em Fase SólidaRESUMO
The first example of the synthesis of new dinucleotide cap analog containing 2('),3(')-diacetyl group on m(7)guanosine moiety is described. The desired modified cap analog, m(7,2)(')(,3)(')(-diacetyl)G[5(')]ppp[5(')]G has been obtained by the coupling reaction of triethylamine salt of m(7,2)(')(,3)(')(-diacetyl)GDP with ImGMP in presence of ZnCl2 as a catalyst in 62% yield with high purity. The structure of new cap analog has been confirmed by (1)H and (31)P NMR and mass data.
Assuntos
Guanosina/análogos & derivados , Guanosina/química , Análogos de Capuz de RNA/química , Guanosina/síntese química , Análogos de Capuz de RNA/síntese química , RNA Mensageiro/químicaRESUMO
Photoaffinity labeling is widely applied to demonstrate targets of small molecule ligands. In this paper, biotin photoaffinity labeled molecule with propargyl group 1 has been designed and synthesized, followed it's labeling of N2-acetyl-2'-O-propargyl guanosine 9 by "click chemistry". This technology presents delight development potential in labeling of second messenger cyclic nucleotide, antisense oligonucleotide or siRNA.
Assuntos
Química Click , Guanosina/química , Guanosina/síntese química , Marcadores de Fotoafinidade , Biotina/química , LigantesRESUMO
A series of lipophilic nucleosides comprising natural and non-natural bases that are π-conjugated to a short oligophenylene-ethynylene fragment has been synthesized. These bases comprise guanosine, isoguanosine, and 2-aminoadenosine as purine heterocycles, and cytidine, isocytosine and uridine as complementary pyrimidine bases. The hydrogen-bonding dimerization and association processes between complementary bases were also studied by (1)H NMR and absorption spectroscopy in order to obtain the relevant association constants.
Assuntos
Nucleosídeos/química , Polímeros/química , Adenosina/análogos & derivados , Adenosina/síntese química , Adenosina/química , Citosina/análogos & derivados , Citosina/síntese química , Citosina/química , Dimerização , Guanosina/síntese química , Guanosina/química , Ligação de Hidrogênio , Nucleosídeos/síntese química , Polímeros/síntese química , Uridina/síntese química , Uridina/químicaRESUMO
Oligoribonucleotides containing 3'-S-phosphorothiolate linkages possess properties that can reveal deep mechanistic insights into ribozyme-catalyzed reactions. "Photocaged" 3'-S- RNAs could provide a strategy to stall reactions at the chemical stage and release them after assembly steps have occurred. Toward this end, we describe here an approach for the synthesis of 2'-O-(o-nitrobenzyl)-3'-thioguanosine phosphoramidite starting from N(2)-isobutyrylguanosine in nine steps with 10.2% overall yield. Oligonucleotides containing the 2'-O-(o-nitrobenzyl)-3'-S-guanosine nucleotide were then constructed, characterized, and used in a nuclear pre-mRNA splicing reaction.
Assuntos
Guanosina/análogos & derivados , Sondas Moleculares/síntese química , Oligorribonucleotídeos/química , Compostos Organofosforados/síntese química , Fosfatos/síntese química , RNA Catalítico/química , Guanosina/síntese química , Guanosina/química , Sondas Moleculares/química , Conformação de Ácido Nucleico , Fosfatos/química , Splicing de RNARESUMO
Nucleoside phosphonate analogs are an important class of antiviral drugs for the treatment of HIV and HBV. The most recent nucleoside phosphonate to progress to clinical development is GS-9131, a cyclic nucleoside phosphonate (CNP). This unit contains procedures for the synthesis of the parent CNP 2'-Fd4AP (GS-9148) and selected monoamidate and bisamidate prodrugs, including the monoamidate clinical prodrug GS-9131. The first basic protocol of this unit details improved procedures for the preparation of 2'-Fd4AP and related phosphonate esters by introduction of a hydroxylmethyl phosphonate ester regioselectively and stereoselectively onto a furanose core via a glycal intermediate. The method described is believed to be robust and flexible, allowing for a variety of analogs with other nucleobases or furanose 2'-ring substitutions to be prepared. The preparation of monoamidate and bisamidate prodrugs either on the phosphonate diacid or its monophenyl ester is then described in the second and third basic protocols of this unit.
Assuntos
Adenina/análogos & derivados , Fármacos Anti-HIV , Guanosina/análogos & derivados , HIV-1 , Pró-Fármacos , Adenina/síntese química , Adenina/química , Animais , Fármacos Anti-HIV/síntese química , Fármacos Anti-HIV/química , Guanosina/síntese química , Guanosina/química , Humanos , Pró-Fármacos/síntese química , Pró-Fármacos/químicaRESUMO
The synthesis of a terpyridine-guanosine ligand and its reaction with copper(II) to yield a new [2 + 2] metallo-rectangle is reported. The metallo-rectangle was characterized by single crystal X-ray diffraction and the structure showed significant intramolecular π-π stacking interactions between the two terpyridine moieties of the molecule. This prompted us to investigate the magnetic properties of the new di-copper(II) assembly which displayed ferromagnetic interactions in the solid state.