RESUMO
OBJECTIVE: The purpose of these studies was to investigate the uptake of atrazine across the nasal mucosa to determine whether direct transport to the brain through the olfactory epithelium is likely to occur. These studies were undertaken to provide important new information about the potential for the enhanced neurotoxicity of herbicides following nasal inhalation. MATERIALS AND METHODS: Transport of atrazine from aqueous solution and from commercial atrazine-containing herbicide products was assessed using excised nasal mucosal tissues. The permeation rate and the role of membrane transporters in the uptake of atrazine across the nasal mucosa were also investigated. Histological examination of the nasal tissues was conducted to assess the effects of commercial atrazine-containing products on nasal tissue morphology. RESULTS: Atrazine showed high flux across both nasal respiratory and olfactory tissues, and efflux transporters were found to play an essential role in limiting its uptake at low exposure concentrations. Commercial atrazine-containing herbicide products showed remarkably high transfer across the nasal tissues, and histological evaluation showed significant changes in the morphology of the nasal epithelium following exposure to the herbicide products. DISCUSSION: Lipophilic herbicides such as atrazine can freely permeate across the nasal mucosa despite the activity of efflux transporters. The adjuvant compounds in commercial herbicide products disrupt the nasal mucosa's epithelial barrier, resulting in even greater atrazine permeation across the tissues. The properties of the herbicide itself and those of the formulated products play crucial roles in the potential for the enhanced neurotoxicity of herbicides following nasal inhalation.
Assuntos
Atrazina , Herbicidas , Mucosa Nasal , Atrazina/toxicidade , Atrazina/farmacocinética , Herbicidas/toxicidade , Herbicidas/farmacocinética , Mucosa Nasal/efeitos dos fármacos , Mucosa Nasal/metabolismo , Animais , Proteínas de Membrana Transportadoras/metabolismo , Masculino , Administração Intranasal , Absorção Nasal/efeitos dos fármacosRESUMO
The co-occurrence of glyphosate (GLP) and aminomethylphosphonic acid (AMPA) in contaminated water, soil, sediment and plants is a cause for concern due to potential threats to the ecosystem and human health. A major route of exposure is through contact with contaminated soil and consumption of crops containing GLP and AMPA residues. However, clay-based sorption strategies for mixtures of GLP and AMPA in soil, plants and garden produce have been very limited. In this study, in vitro soil and in vivo genetically modified corn models were used to establish the proof of concept that the inclusion of clay sorbents in contaminated soils will reduce the bioavailability of GLP and AMPA in soils and their adverse effects on plant growth. Effects of chemical concentration (1-10 mg/kg), sorbent dose (0.5%-3% in soil and 0.5%-1% in plants) and duration (up to 28 days) on sorption kinetics were studied. The time course results showed a continuous GLP degradation to AMPA. The inclusion of calcium montmorillonite (CM) and acid processed montmorillonite (APM) clays at all doses significantly and consistently reduced the bioavailability of both chemicals from soils to plant roots and leaves in a dose- and time-dependent manner without detectable dissociation. Plants treated with 0.5% and 1% APM inclusion showed the highest growth rate (p ≤ 0.05) and lowest chemical bioavailability with up to 76% reduction in roots and 57% reduction in leaves. Results indicated that montmorillonite clays could be added as soil supplements to reduce hazardous mixtures of GLP and AMPA in soils and plants.
Assuntos
Bentonita , Bioacumulação , Herbicidas , Organofosfonatos , Poluentes do Solo , Zea mays , Humanos , Bentonita/química , Argila/química , Ecossistema , Herbicidas/análise , Herbicidas/química , Herbicidas/farmacocinética , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/farmacocinética , Zea mays/química , Zea mays/fisiologia , Organofosfonatos/análise , Organofosfonatos/química , Organofosfonatos/farmacocinética , Plantas Geneticamente Modificadas/química , Plantas Geneticamente Modificadas/fisiologia , Bioacumulação/fisiologia , GlifosatoRESUMO
Weeds, pests, and pathogens are among the pre-harvest constraints in rice farming across rice-growing countries. For weed management, manual weeding and herbicides are widely practiced. Among the herbicides, glyphosate [N-(phosphonomethyl) glycine] is a broad-spectrum systemic chemical extensively used in agriculture. Being a competitive structural analog to phosphoenolpyruvate, it selectively inhibits the conserved 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme required for the biosynthesis of aromatic amino acids and essential metabolites in eukaryotes and prokaryotes. In the present study, we investigated the antifungal and defense elicitor activity of glyphosate against Magnaporthe oryzae on transgenic-rice overexpressing a glyphosate-resistance OsEPSPS gene (T173I + P177S; TIPS OsmEPSPS) for blast disease management. The glyphosate foliar spray on OsmEPSPS transgenic rice lines showed both prophylactic and curative suppression of blast disease comparable to a blasticide, tricyclazole. The glyphosate displayed direct antifungal activity on Magnaporthe oryzae as well as enhanced the levels of antioxidant enzymes and photosynthetic pigments in rice. However, the genes associated with phytohormones-mediated defense (OsPAD4, OsNPR1.3, and OsFMO) and innate immunity pathway (OsCEBiP and OsCERK1) were found repressed upon glyphosate spray. Altogether, the current study is the first report highlighting the overexpression of a crop-specific TIPS mutation in conjugation with glyphosate application showing potential for blast disease management in rice cultivation.
Assuntos
Antifúngicos/farmacologia , Ascomicetos/patogenicidade , Resistência a Herbicidas/genética , Herbicidas/farmacocinética , Oryza/genética , Oryza/microbiologia , Oryza/fisiologia , Doenças das Plantas/prevenção & controle , Ascomicetos/efeitos dos fármacos , Produtos Agrícolas/genética , Produtos Agrícolas/microbiologia , Produtos Agrícolas/fisiologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Variação Genética , Genótipo , Doenças das Plantas/genética , Plantas Geneticamente Modificadas/genéticaRESUMO
Currently, the authorisation process for plant protection products (PPPs) relies on the testing of acute and topological toxicity only. Contrastingly, the evaluation of active substances includes a more comprehensive set of toxicity studies. Nevertheless, mixture effects of active ingredients and co-formulants may result in increased toxicity. Therefore, we investigated effects of surface active co-formulants on the toxicity of two PPPs focussing on qualitative and quantitative toxicokinetic effects on absorption and secretion. The respective products are based on the active substances abamectin and fluroxypyr-meptyl and were tested for cytotoxicity in the presence or absence of the corresponding surfactants and co-formulants using Caco-2 cells. In addition, the effect of co-formulants on increased cellular permeation was quantified using LC-MS/MS, while potential kinetic mixture effects were addressed by fluorescence anisotropy measurements and ATPase assays. The results show that surface active co-formulants significantly increase the cytotoxicity of the investigated PPPs, leading to more than additive mixture effects. Moreover, analytical investigations show higher efflux ratios of both active substances and the metabolite fluroxypyr upon combination with certain concentrations of the surfactants. The results further point to a significant and concentration-dependent inhibition of Pgp transporters by most of the surfactants as well as to increased membrane fluidity. Altogether, these findings strongly support the hypothesis that surfactants contribute to increased cytotoxicity of PPPs and do so by increasing the bioavailability of the respective active substances.
Assuntos
Glicolatos/toxicidade , Herbicidas/toxicidade , Inseticidas/toxicidade , Ivermectina/análogos & derivados , Disponibilidade Biológica , Células CACO-2 , Cromatografia Líquida , Polarização de Fluorescência , Glicolatos/administração & dosagem , Glicolatos/farmacocinética , Herbicidas/administração & dosagem , Herbicidas/farmacocinética , Humanos , Inseticidas/administração & dosagem , Inseticidas/farmacocinética , Ivermectina/administração & dosagem , Ivermectina/farmacocinética , Ivermectina/toxicidade , Tensoativos/química , Espectrometria de Massas em TandemRESUMO
Chloro-s-triazines-atrazine, cyanazine, propazine, simazine, and terbuthylazine-are structurally similar herbicides, differing only in specific s-triazine4-and 6-N alkyl substituents. It is generally regarded that their toxicokinetics, such as, metabolic pathways, biological effects and toxicities, also share more similar features than the differences. Consequently, it is useful to compare their characteristics to potentially find useful structure-activity relationships or other similarities or differences regarding different active compounds, their metabolites, and biological effects including toxic outcomes. The ultimate goal of these exercises is to apply the summarized knowledge-as far as it is possible regarding a patchy and often inadequate database-to cross the in vitro-in vivo and animal-human borders and integrate the available data to enhance toxicological risk assessment for the benefit of humans and ecosystems.
Assuntos
Herbicidas/farmacocinética , Herbicidas/toxicidade , Triazinas/farmacocinética , Triazinas/toxicidade , Animais , Exposição Ambiental/análise , Exposição Ambiental/normas , Regulamentação Governamental , HumanosRESUMO
Different technologies to prepare long term pesticide forms include polymer coating, preparing composites and encapsulating pesticides in nanoparticles. A simple and low-cost method was proposed to obtain slow-release formulations by co-extrusion of a pesticide with a biodegradable polymer at a temperature above the melting points of both components. A herbicide metribuzin and low-melting polyester poly-ε-caprolactone were chosen for this work. Formulations containing 10%, 20%, and 40% herbicide were prepared. During 7 days of their exposition in water, it was released from 81% to 96% of initially loaded metribuzin; the highest release was detected for 40%-loaded forms. Biodegradation of the constructs and pesticide release were further studied in the model soil. Degradation rates of the specimens increased with an increase in pesticide content, from 9% to 20% over 14 weeks for the 10%/20%-loaded and the 40%-loaded specimens, respectively. The release of metribuzin reached, respectively, 37-38% and 55%. The herbicide content in soil was lower due to its partial degradation in soil; it reached 23-25% and 33%, respectively, from initially loaded into the polymer matrix. Release kinetics of metribuzin in water as in soil best fitted the First-order model. The used approach is promising for obtaining long-term release formulations for soil applications.
Assuntos
Caproatos/química , Herbicidas/química , Lactonas/química , Poliésteres/química , Poluentes do Solo/química , Triazinas/química , Biodegradação Ambiental , Caproatos/análise , Caproatos/farmacocinética , Preparações de Ação Retardada , Composição de Medicamentos , Herbicidas/análise , Herbicidas/farmacocinética , Cinética , Lactonas/análise , Lactonas/farmacocinética , Poliésteres/análise , Poliésteres/farmacocinética , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/farmacocinética , Triazinas/análise , Triazinas/farmacocinéticaRESUMO
By extending our Paraquat (PQ) work to include primates we have implemented a modelling and simulation strategy that has enabled PQ pharmacokinetic data to be integrated into a single physiologically based pharmacokinetic (PBPK) model that enables more confident extrapolation to humans. Because available data suggested there might be differences in PQ kinetics between primates and non-primates, a radiolabelled study was conducted to characterize pharmacokinetics and excretion in Cynomolgus monkeys. Following single intravenous doses of 0.01 or 0.1 mg paraquat dichloride/kg bw, plasma PQ concentration-time profiles were dose-proportional. Excretion up to 48 h (predominantly urinary) was 82.9%, with ca. 10% remaining unexcreted. In vitro blood binding was similar across Cynomolgus monkeys, humans and rat. Our PBPK model for the rat, mouse and dog, employing a single set of PQ-specific parameters, was scaled to Cynomolgus monkeys and well represented the measured plasma concentration-time profiles over 14 days. Addition of a cartilage compartment to the model better captured the percent remaining in the monkeys at 48 h, whilst having negligible effect on model predictions for the other species. The PBPK model performed well for all four species, demonstrating there is little difference in PQ kinetics between non-primates and primates enabling a more confident extrapolation to humans. Scaling of the PBPK model to humans, with addition of a human-specific dermal submodel based on in vitro human dermal absorption data, provides a valuable tool that could be employed in defining internal dosimetry to complement human health risk assessments.
Assuntos
Herbicidas/farmacocinética , Modelos Biológicos , Paraquat/farmacocinética , Animais , Simulação por Computador , Herbicidas/administração & dosagem , Herbicidas/sangue , Herbicidas/toxicidade , Humanos , Infusões Intravenosas , Eliminação Intestinal , Macaca fascicularis , Paraquat/administração & dosagem , Paraquat/sangue , Paraquat/toxicidade , Ratos , Eliminação Renal , Medição de Risco , Absorção Cutânea , Especificidade da Espécie , Distribuição Tecidual , ToxicocinéticaRESUMO
Paraquat dichloride (PQ) is a non-selective herbicide which has been the subject of numerous toxicology studies over more than 50 years. This paper describes the development of a physiologically-based pharmacokinetic (PBPK) model of PQ kinetics for the rat, mouse and dog, firstly to aid the interpretation of studies in which no kinetic measurements were made, and secondly to enable the future extension of the model to humans. Existing pharmacokinetic data were used to develop a model for the rat and mouse. Simulations with this preliminary model were then used to identify key data gaps and to design a new blood binding study to reduce uncertainty in critical aspects of the model. The new data provided evidence to support the model structure, and its predictive performance was then assessed against dog and rat datasets not used in model development. The PQ-specific model parameters are the same for all three species, with only the physiological parameters varying between species. This consistency across species provides a strong basis for extrapolation to other species, as demonstrated here for the dog. The model enables a wide range of PQ data to be linked together to provide a broad understanding of PQ pharmacokinetics in rodents and the dog, showing that the key aspects of PQ kinetics in these species are understood and adequately encapsulated within the model.
Assuntos
Herbicidas/farmacocinética , Modelos Biológicos , Paraquat/farmacocinética , Animais , Simulação por Computador , Cães , Herbicidas/sangue , Herbicidas/toxicidade , Eliminação Intestinal , Camundongos , Paraquat/sangue , Paraquat/toxicidade , Ligação Proteica , Ratos , Eliminação Renal , Medição de Risco , Especificidade da Espécie , Distribuição Tecidual , ToxicocinéticaRESUMO
Fertiactyl® is a foliar fertilizer with the potential to minimize the phytotoxicity effects caused by glyphosate drift in eucalyptus plants. As the interactions of the glyphosate and Fertiactyl® in tank mix on the plant behavior are not yet known, the objective was to evaluate the absorption and translocation of 14C-glyphosate, applied isolated and mixed in tank with Fertiactyl®, in young eucalyptus plants (clone I-144, Eucalyptus urophylla x E. grandis). The addition of Fertiactyl® to the mixture of 14C-glyphosate reduced the absorption by 94.3% in relation to the total absorbed at the end of the evaluation compared to plants treated only with 14C-glyphosate, i.e., Fertiactyl® protected the eucalyptus plants of the glyphosate intoxication by drift. The translocation rates from the treated leaves to the rest of the shoots and roots were low (<2% of the total recovered) in both treatments, suggest that restricted translocation is a mechanism of natural tolerance to glyphosate in plants of clone I-144. It is concluded that Fertiactyl®, mixed in the solution with glyphosate, protects young eucalyptus plants against glyphosate drift by reducing the amount of herbicide absorbed.
Assuntos
Eucalyptus/efeitos dos fármacos , Fertilizantes , Glicina/análogos & derivados , Herbicidas/farmacocinética , Radioisótopos de Carbono/farmacocinética , Eucalyptus/metabolismo , Glicina/farmacocinética , Glicina/toxicidade , Herbicidas/toxicidade , Folhas de Planta/efeitos dos fármacos , Brotos de Planta/efeitos dos fármacos , Distribuição Tecidual , GlifosatoRESUMO
The application of glyphosate to coffee crops can cause injuries to plants. Fertiactyl® foliar fertilizer reduces injuries when mixed with glyphosate; however, it is important to establish which mechanisms are responsible for this protective action. This study aimed to evaluate the absorption and translocation of glyphosate applied separately and in mixture with Fertiactyl® in coffee seedlings. Absorption and translocation were performed with 14C-glyphosate applied separately and in mixture with Fertiactyl® at 0, 6, 12, 24, 48, 96, and 144 hours after application (HAA). Most of the 14C-glyphosate applied to coffee seedlings was not absorbed. The 14C-glyphosate applied separately had a higher absorption by coffee seedlings (6.5%) than in a mixture with Fertiactyl® (2.7%) at 144 HAA. The maximum translocation of the 14C-glyphosate applied separately was 0.69% at 81.2 HAA and in mixture with Fertiactyl® was 0.41% at 41.2 HAA. The treated leaves retained a higher percentage of 14C-glyphosate when applied separately (5.6% at 144 HAA) than in a mixture with Fertiactyl® (2.2% at 144 HAA). Low translocation (<1%) for the rest of the plant shoots was observed both for the 14C-glyphosate applied separately and in combination with Fertiactyl®. Therefore, Fertiactyl® decreased the absorption and translocation of 14C-glyphosate in coffee seedlings.
Assuntos
Coffea/metabolismo , Fertilizantes , Glicina/análogos & derivados , Herbicidas/farmacocinética , Absorção Fisiológica , Transporte Biológico , Glicina/farmacocinética , Folhas de Planta/metabolismo , Plântula/metabolismo , GlifosatoRESUMO
Weeds are notorious plant species exhibiting a harmful impact on crops. Biological weed control is an efficient and environmentally friendly technique, usually constitutes naturally derived compounds, including bioherbicidal metabolites produced by Streptomyces sp. The isolation and structural identification of phytotoxic compounds from Streptomyces have recently been proposed as an effective way to the discovery of novel bioherbicides. In the screening of bioherbicidal agents, isolated Streptomyces strain KRA17-580 demonstrated significant phytotoxic activity against Digitaria ciliaris. Phylogenetic analysis of the 16S rRNA sequence indicated that isolated KRA17-580 is similar to Streptomyces olivochromogenes. The bacterial culture conditions were optimized for temperature, agitation, and initial pH. Streptomyces strain KRA17-580 showed intense phytotoxic activity and high cell mass at an initial pH of 5.5-7.0, more than 150 rpm, and 25-30 °C. The herbicidal compounds isolated from the culture filtrate of strain KRA17-580 were purified by solvent partition, C18, Sephadex LH20 column chromatography, and high-performance liquid chromatography. By 1D-NMR, 2D-NMR, and electrospray ionization mass spectrometry analysis, the 580-H1 and 580-H2 compounds were identified as a cinnoline-4-carboxamide (MW, 173.0490; C9H7N3O2) and cinnoline-4-carboxylic acid (MW, 174.0503; C9H6N2O2), respectively. Only these two herbicidal compounds showed strong phytotoxic activity against D. ciliaris in foliar applications. However, compound 580-H2 was more phytotoxic than 580-H1 and the toxicity was dose-dependent. The herbicidal metabolite KRA17-580 produced by Streptomyces sp. is a new bioherbicidal candidate that may provide a new lead molecule for more efficient phytotoxic compounds.
Assuntos
Herbicidas/química , Herbicidas/farmacocinética , Streptomyces/química , Streptomyces/metabolismo , Cromatografia Líquida de Alta Pressão , Digitaria/efeitos dos fármacos , Digitaria/crescimento & desenvolvimento , Herbicidas/metabolismo , Filogenia , Plantas Daninhas/efeitos dos fármacos , Plantas Daninhas/crescimento & desenvolvimento , Espectrometria de Massas por Ionização por Electrospray , Streptomyces/classificação , Streptomyces/genéticaRESUMO
The main organochlorinated compounds used on agricultural crops are often recalcitrant, affecting nontarget organisms and contaminating rivers or groundwater. Diuron (N-(3,4-dichlorophenyl)-N',N'-dimethylurea) is a chlorinated herbicide widely used in sugarcane plantations. Here, we evaluated the ability of 13 basidiomycete strains of growing in a contaminated culture medium and degrading the xenobiotic. Dissipation rates in culture medium with initial 25 mg/L of diuron ranged from 7.3 to 96.8%, being Pluteus cubensis SXS 320 the most efficient strain, leaving no detectable residues after diuron metabolism. Pycnoporus sanguineus MCA 16 removed 56% of diuron after 40 days of cultivation, producing three metabolites more polar than parental herbicide, two of them identified as being DCPU and DCPMU. Despite of the strong inductive effect of diuron upon laccase synthesis and secretion, the application of crude enzymatic extracts of P. sanguineus did not catalyzed the breakdown of the herbicide in vitro, indicating that diuron biodegradation was not related to this oxidative enzyme.
Assuntos
Basidiomycota/metabolismo , Biodegradação Ambiental , Diurona/farmacocinética , Herbicidas/farmacocinética , Poluentes Químicos da Água/farmacocinética , Agaricales/metabolismo , Basidiomycota/efeitos dos fármacos , Basidiomycota/crescimento & desenvolvimento , Sistema Enzimático do Citocromo P-450/metabolismo , Diurona/toxicidade , Herbicidas/toxicidade , Hidrocarbonetos Clorados/farmacocinética , Hidrocarbonetos Clorados/toxicidade , Lacase/metabolismo , Modelos Biológicos , Nitrogênio/metabolismo , Polyporaceae/metabolismo , Floresta Úmida , Poluentes Químicos da Água/toxicidadeRESUMO
A review of pharmacokinetic and metabolism studies show that triclopyr is well absorbed from the oral route in numerous species (≥80%), primarily as parent compound. Absorption is quite rapid in rats, dogs and human volunteers. Plasma or blood clearance is also rapid (t1/2 3-9 h), except for dog (12-96 h). Systemic exposure is not dose-proportional: in the rat above 20 mg/kg (dietary) or between 3 and 60 mg/kg (gavage), or in dogs above 5 mg/kg, with systemic exposure in human more comparable to rat than dog. Triclopyr is highly bound to protein in rat, dog and human plasma (≥97% at or below 7 µg/mL), indicating that species differences in systemic exposure are not due to differences in the free fraction of this test material in plasma. An in vitro flux study in renal proximal tubule cells showed that net renal transport of triclopyr is in the direction of secretion in rat and human donors, while reabsorption predominated in the dog, possibly via organic anion transporters such as OAT1/3. These results fit well into the framework of utilizing metabolism and toxicokinetics across species and exposure levels to allow for toxicity testing in the most relevant species as well as at proper dose levels.
Assuntos
Glicolatos/farmacocinética , Herbicidas/farmacocinética , Animais , Humanos , Medição de RiscoRESUMO
BACKGROUND: Since the classification of glyphosate as a Group 2A substance "probably carcinogenic to humans" by the IARC in 2015, human health concerns have been raised regarding the exposure of operators, bystanders, and consumers. Urine measurement studies have been conducted, but since toxicokinetic data on glyphosate in humans is lacking, a meaningful interpretation of this data regarding exposure is not possible. OBJECTIVE: This study aims to determine the fraction of glyphosate and AMPA excretion in urine after consuming ordinary food with glyphosate residue, to estimate dietary glyphosate intake. METHODS: Twelve participants consumed a test meal with a known amount of glyphosate residue and a small amount of AMPA. Urinary excretion was examined for the next 48 h. RESULTS: Only 1% of the glyphosate dose was excreted in urine. The urinary data indicated the elimination half-life was 9 h. For AMPA, 23% of the dose was excreted in urine, assuming that no metabolism of glyphosate to AMPA occurred. If all of the excreted AMPA was a glyphosate metabolite, this corresponds to 0.3% of the glyphosate dose on a molar basis. CONCLUSION: This study provides a basis for estimating oral glyphosate intake using urinary biomonitoring data.
Assuntos
Monitoramento Biológico/métodos , Exposição Dietética/análise , Glicina/análogos & derivados , Herbicidas/urina , Organofosfonatos/urina , Resíduos de Praguicidas/urina , Adulto , Biomarcadores/urina , Cicer , Feminino , Farinha/análise , Contaminação de Alimentos/análise , Glicina/análise , Glicina/farmacocinética , Glicina/urina , Herbicidas/análise , Herbicidas/farmacocinética , Humanos , Masculino , Organofosfonatos/análise , Organofosfonatos/farmacocinética , Resíduos de Praguicidas/análise , Resíduos de Praguicidas/farmacocinética , GlifosatoRESUMO
In this study, the preparation and characterization of haloxyfop-R-methyl herbicide loaded in poly(methylmethacrylate) (PMMA) nano-capsules by emulsion polymerization and its release behavior were investigated. The chemical characterizations of PMMA/haloxyfop-R-methyl nano-capsules were confirmed by FT-IR spectroscopy method, and the surface morphology was studied by field emission scanning electron microscopy and transmission electron microscopy. Also, the herbicide loading and encapsulation efficiency were analyzed for the herbicide-loaded nano-capsules. The release rate of PMMA/haloxyfop-R-methyl nano-capsules was determined by UV-visible spectroscopy. The thermal properties and thermal stability of nano-capsules were explored by the thermal gravimetric analysis method. The diameter of the nano-capsules was in the range of 100-300 nm. Increasing the amount of herbicide in nano-formulations significantly affected the surface of the nano-capsules and reduced their surface smoothness. Triton-X100 was identified as the best surfactant for the preparation of nano-capsules, and the sample containing the lowest herbicide content showed the best performance in terms of encapsulation and loading efficiency. This sample showed a steady-state release rate during the six days.
Assuntos
Herbicidas/farmacocinética , Nanoestruturas/química , Polimetil Metacrilato/química , Piridinas/química , Piridinas/farmacocinética , Cápsulas , Preparações de Ação Retardada , Emulsões/química , Herbicidas/química , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Octoxinol/química , Polimerização , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , TermogravimetriaRESUMO
Although there are many reports on the dangers posed by glyphosate, this herbicide is still one of the most popular and widely used total weed killers. Therefore, great effort should be made to minimize exposure to this herbicide and limit its losses into the environment. The aim of this study was to prepare a new formulation consisted of various molecular weight chitosans with glyphosate and their evaluation toward active substance release, phytotoxicity, and preliminary herbicidal efficiency. The phytotoxicity study of the obtained chitosan/glyphosate formulations was determined based on the Organisation for Economic Co-operation and Development 208 guideline. Among all tested formulations evaluated for phytotoxicity, that containing the highest molecular weight of chitosan was found to be the least toxic, showing simultaneously the most effective herbicidal activity against selected weeds. The release rate of glyphosate from the obtained formulations was dependent on the molecular weight and viscosity of chitosan.
Assuntos
Quitosana/química , Glicina/análogos & derivados , Herbicidas/química , Herbicidas/farmacologia , Quitosana/farmacologia , Glicina/química , Glicina/farmacocinética , Glicina/farmacologia , Herbicidas/farmacocinética , Peso Molecular , Plantas Daninhas/efeitos dos fármacos , GlifosatoRESUMO
This study explored the characteristics of a newly isolated glyphosate (GLYP)-degrading bacterium Providencia rettgeri GDB 1, for GLYP bioremediation. Due to the serial selection pressure of high GLYP concentrations for enriched isolation, this highly tolerant GLYP biodegrader shows very promising capabilities for GLYP removal (approximately 71.4% degradation efficiency) compared to previously reported strains. High performance liquid chromatography analyses showed aminomethylphosphonic acid (AMPA) rather than sarcosine (SAR) to be the sole intermediate of GLYP decomposition via the AMPA formation pathway. Moreover, GLYP biodegradation was biochemically favorable in aerobic cultures due to its strong growth-associated characteristics. To the best of our knowledge, this is the first report to indicate that bacterial strains in the Providencia genus could demonstrate highly promising GLYP-degrading characteristics in environments with high GLYP contents.
Assuntos
Glicina/análogos & derivados , Herbicidas/farmacocinética , Providencia/metabolismo , Poluentes Químicos da Água/farmacocinética , Adaptação Biológica , Agricultura/métodos , Agroquímicos/isolamento & purificação , Agroquímicos/farmacocinética , Agroquímicos/toxicidade , Biodegradação Ambiental , Cromatografia Líquida de Alta Pressão/métodos , Glicina/isolamento & purificação , Glicina/farmacocinética , Glicina/toxicidade , Herbicidas/isolamento & purificação , Herbicidas/toxicidade , Humanos , Isoxazóis/química , Isoxazóis/metabolismo , Redes e Vias Metabólicas , Providencia/efeitos dos fármacos , Providencia/enzimologia , Providencia/crescimento & desenvolvimento , Sarcosina/química , Sarcosina/metabolismo , Tetrazóis/química , Tetrazóis/metabolismo , Poluentes Químicos da Água/isolamento & purificação , Poluentes Químicos da Água/toxicidade , Purificação da Água/métodos , GlifosatoAssuntos
Herbicidas/toxicidade , Imidazóis/toxicidade , Niacina/análogos & derivados , Adulto , Escala de Coma de Glasgow , Parada Cardíaca/induzido quimicamente , Herbicidas/sangue , Herbicidas/farmacocinética , Herbicidas/urina , Humanos , Imidazóis/sangue , Imidazóis/farmacocinética , Imidazóis/urina , Masculino , Niacina/sangue , Niacina/farmacocinética , Niacina/toxicidade , Niacina/urinaRESUMO
Organic soil amendments can be useful for improving degraded soil, but this increase in organic matter (OM) may influence adsorption of herbicides subsequently applied to the treated soil, even though the particle size of amendments and their nature differ from typical soil OM. In this study, a batch equilibrium method was used to measure adsorption of five herbicides following application to two organic media, wood pulp and sawdust, comparing these with two cropping soils. Herbicide adsorption, quantified by distribution coefficients (kd), was much higher in the two organic media than in the cropping soils. The increases in adsorption were strongly correlated to the percentage of organic carbon. When the kd was normalized to adsorption coefficients corrected for OM content (koc), variation in results between the media was greatly reduced, indicating that OM is an important factor influencing adsorption in these media. The results of this study suggest that herbicides will be less effective when applied to soils in which sawdust and wood pulp have been added. Using organic amendments to remediate soil will increase adsorption of pesticides, reducing their bio-availability and efficacy, but also reducing their tendency to leach into root zones of deep-rooted crops and into groundwater.
Assuntos
Recuperação e Remediação Ambiental/métodos , Agricultura Florestal/métodos , Herbicidas/química , Poluentes do Solo/química , Resíduos , Adsorção , Disponibilidade Biológica , Produtos Agrícolas , Herbicidas/análise , Herbicidas/farmacocinética , Nova Zelândia , Tamanho da Partícula , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/farmacocinética , MadeiraRESUMO
Physiologically-based toxicokinetic (PBTK) models are mathematical representations of chemical absorption, distribution, metabolism and excretion (ADME) in animals. Each parameter in a PBTK model describes a physiological, physicochemical or biochemical process that affects ADME. Distributions can be assigned to the model parameters to describe population variability and uncertainty. In this study to assess potential crop sprayer operator exposure to the herbicide haloxyfop, a permeability-limited PBTK model was constructed with parameter uncertainty and variability, and calibrated using Bayesian analysis via Markov chain Monte Carlo methods. A hierarchical statistical model was developed to reconstruct operator exposure using available measurement data: experimentally determined octanol/water partition coefficient, mouse and human toxicokinetic data as well as human biomonitoring data from seven operators who participated in a field study. A chemical risk assessment was performed by comparing the estimated systemic exposure to the acceptable operator exposure level (AOEL). The analysis suggested that in one of the seven operators, the model estimates systemic exposure to haloxyfop of 49.04⯱â¯10.19 SD µg/kg bw in relation to an AOEL of 5.0 µg/kg bw/day. This does not represent a safety concern as this predicted exposure is well within the 100-fold uncertainty factor applied to the No Observed Adverse Effect Level (NOAEL) in animals. In addition, given the availability of human toxicokinetic data, the 10x uncertainty factor for interspecies differences in ADME could be reduced (EFSA, 2006). Thus the AOEL could potentially be raised tenfold from 5.0 to 50.0 µg/kg bw/day.