Wound healing activity in rabbits and antimicrobial activity of Hibiscus hirtus ethanolic extract

Generally, Plants have immense potential in the wounds management and treatment.In Chinese herbology, Hibiscus plant is a potent herb and have a good medicinal values but not scientifically approached. The present study aims to investigate the wound healing and antimicrobial activity of ethanolic extract of Hibiscus hirtus Linn.(HH). Wound healing activity was carried out by excision, incision and burn wound models. Antimicrobial activity was determined by cup plate method. Healing rate was assessed from the rate of wound contraction, epithelialization rate, tensile strength, hexosamine and hydroxyproline content. From the obtained results, it was indicated that the wound contraction and increased tensile strength of Hibiscus hirtus extract exhibits potent wound healing capacity. Hexosamine and hydroxyproline expression were also correlative with the pattern of healing observed. Histological observation indicates that the wounds treated with Hibiscus hirtus extract and povidone iodine have reduced scar formation and enhances fibroblast proliferation, angiogenesis, keratinization and epithelialization. The Hibiscus hirtus extract has excellent antimicrobial activity against the various organisms. Wound healing activity of our ethanolic extract of Hibiscus hirtus has shown the good effect which has proved by different physical, histological, biochemical parameters. Significant antimicrobial activity shown may be due to major active constituents present in plant.

Hibiscus Sabdariffa L. Flowers and Olea Europea L. Leaves Extract-Based Formulation for Hypertension Care: In Vitro Efficacy and Toxicological Profile.

Olea europaea L. leaves extract (Oe) and Hybiscus sabdariffa L. flowers extract (Hs) have calcium antagonistic properties. Aim of this work was to study the cardiovascular effects of Pres Phytum(®), a nutraceutical formulation containing a mixture of the two extracts and the excipients, and investigate its possible off-target effects, using in vitro biological assays on guinea pig isolated organs. Cardiovascular effects were assessed using guinea pig atria and aorta. The effects of Pres Phytum on spontaneous gastrointestinal, urinary, and respiratory tracts smooth muscle contractility were evaluated. Pres Phytum exerted a vasorelaxant effect (IC50 = 2.38 mg/mL) and a negative chronotropic effect (IC50 = 1.04 mg/mL) at concentrations lower than those producing smooth muscle spontaneous contractility alterations in the other organs. Compared to Pres Phytum, the mixture did not exert negative inotropic activity, while it maintained a negative chronotropic efficacy (IC50 = 1.04 mg/mL). These experimental data suggest a possible nutraceutical use of this food supplement for the management of preclinical hypertension.

Hibiscus cannabinus: efeitos nos parâmetrosnutricionais e bioquímicos de ratosdislipidêmicos / Hibiscus cannabinus: effects on nutritional parameters and biochemicalof rats dyslipidemic

A planta Hibiscus cannabinus (Hc) é utilizada na medicina popular para tratamentos inflamatórios. Poucas são aspesquisas que caracterizam o potencial anti-hiperlipidêmico desta planta, principalmente das flores. O objetivo dopresente estudo foi avaliar o efeito do extrato seco das flores de Hc nos parâmetros nutricionais e bioquímicos de ratosdislipidêmicos. Foram utilizados 21 ratos machos e adultos, divididos em três grupos: Grupo C - dieta padrão; GrupoD - dieta hiperlipídica; Grupo DH - dieta hiperlipídica acrescida do extrato seco das flores de Hc. O estudo teve duraçãode sete semanas. Em duas semanas os animais dos Grupos D e DH receberam dieta hiperlipídica para indução dadislipidemia, posteriormente o Grupo DH recebeu tratamento com Hc (500mg/kg peso) por cinco semanas. O GrupoC recebeu dieta padrão (AIN-93M) durante o estudo. Os animais permaneceram em ambiente controlado, sendocoletado peso corporal, consumo alimentar, ingestão hídrica, excreção urinária e fecal. Ao final do estudo o sanguefoi utilizado para determinações bioquímicas e o peso dos órgãos para comparação entre grupos. O Grupo DH, na7ª semana de estudo, apresentou menor consumo alimentar comparado ao Grupo C e maior excreção fecal na 2ª e4ª semanas, em comparação aos Grupos C e D. As variáveis peso corporal, ingestão hídrica e excreção urinária nãosofreram influência do extrato das flores de Hc. A dieta dislipidêmica elevou o peso do fígado nos Grupos D e DH.O Hc não foi eficiente em manter os parâmetros de normalidade das variáveis estudadas, com exceção do consumoalimentar e excreção fecal.

The plant Hibiscus cannabinus (Hc) is used in folk medicine for inflammatory treatments. There are few studies thatcharacterize the anti-hyperlipidemic potential of this plant, mainly flowers. The aim of this study was to evaluate theeffect of dry extract of Hc flowers in nutritional and biochemical parameters of dyslipidemic mice. 21 male rats andadults were divided into three groups: Group C - standard diet; Group D - fat diet; DH Group - plus fat diet dry extractof Hc flowers. The study lasted seven weeks. In two weeks the animals in Groups D and DH received high fat diet forinduction of dyslipidemia, then the DH group received treatment with Hc (500mg / kg weight) for five weeks. Group Creceived standard diet (AIN-93M) during the study. The animals were kept in a controlled environment, being collectedbody weight, food intake, water intake, urinary and fecal excretion. At the end of the study the blood was used forbiochemical determinations and weight of organs for comparison between groups. The DH Group, the 7th week of thestudy, showed lower food consumption compared to Group C and increased fecal excretion in the 2nd and 4th weeks,compared to Groups C and D. The variables body weight, water intake and urinary excretion were not influenced theHc extract of flowers. The dyslipidemic diet increased liver weight in Groups D and DH. The Hc was not effective inmaintaining the normal parameters of the variables studied, with the exception of food and fecal excretion consumption.

Toxicity studies of the water extract from the calyces of Hibiscus sabdariffa L. in rats.

Acute and chronic toxicities of the water extract from calyces of Hibiscus sabdariffa were studied in male and female rats. After 14 days of a single oral administration of test substance 5,000 mg/kg body weight, measurement of the body and organ weights, necropsy and health monitoring were performed. No signs and differences of the weights or behaviour compared to the control rats were observed. The results indicated that the single oral administration of H. sabdariffa extract in the amount of 5,000 mg/kg body weight does not produce acute toxicity. The chronic toxicity was determined by oral feeding both male and female rats daily with the extract at the doses of 50, 100, and 200 mg/kg body weight for 270 days. The examinations of signs, animal behaviour and health monitoring showed no defects in the test groups compared to the control groups. Both test and control groups (day 270th) and satellite group (day 298th) were analysed by measuring their final body and organ weights, taking necropsy, and examining haematology, blood clinical chemistry, and microanatomy. Results showed no differences from the control groups. Overall, our study demonstrated that an oral administration of H. sabdariffa extract at the doses of 50, 100 and 200 mg/kg body weight for 270 days does not cause chronic toxicity in rat.