RESUMO
Synthetic materials used in dentistry may trigger various inflammatory responses. In order to evaluate biocompatibility, standardized implants of Calcium Hydroxide (CH), Glass Ionomer Cement (GIC) and Light-activated Dental Adhesive (LDA) were surgically introduced into Wistar rats' back bone. Six (experimental) animal groups, five each, and two Sham (S) groups were studied after 15 and 30 days from surgery. In each animal, the density of mast cells and interstitial fibrosis volume was evaluated by quantitative light microscopy. In addition, the interaction between the disk material and its fibrous capsule was evaluated by scanning electron microscopy. The density of mast cells per area (N(A)[mast cells]) was lower in CH group than in LDA group. GIC group displayed N(A)[mast cells] results intermediate between CH and LDA groups (p<0.05). The smallest interstitial fibrosis volume density (Vv[f]) was observed in CH group, then in GIC group, while the greatest in LDA group. After 30 days, the fibrosis in LDA group was 30% higher than in CH group (p<0.05). In S group, discreet fibrosis restricted to surgical area was present, with few mast cells near the vessels. Significant interaction between fibrous capsule and the surrounding disk material was most evident in CH group. The implanted materials induced mast cell migration, distinct fibrosis development, suggesting that CH is the most biocompatible material among those tested.
Assuntos
Hidróxido de Cálcio/metabolismo , Cimentos de Ionômeros de Vidro/metabolismo , Mastócitos/metabolismo , Cimentos de Resina/metabolismo , Animais , Materiais Biocompatíveis , Resinas Compostas/metabolismo , Matriz Extracelular/metabolismo , Humanos , Masculino , Mastócitos/imunologia , Teste de Materiais , Próteses e Implantes , Ratos , Ratos Wistar , Tela Subcutânea/metabolismo , Tela Subcutânea/ultraestruturaRESUMO
The biological and bacteriological action of calcium hydroxide confer to its current success as an intracanal dressing. For this reason the mechanism of action of calcium and hydroxyl ions on tissue and bacteria deserves further study. The objective of the present paper is to analyze and discuss the mechanism of action of calcium and hydroxyl ions on anaerobic bacteria, starting from the isolated study of the influence of pH on these bacteria , as well as the mechanism of action of calcium hydroxide on tissue.
Assuntos
Bactérias Anaeróbias/efeitos dos fármacos , Hidróxido de Cálcio/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Radical Hidroxila/farmacologia , Irrigantes do Canal Radicular/farmacologia , Bactérias Anaeróbias/enzimologia , Bactérias Anaeróbias/ultraestrutura , Cálcio/farmacologia , Hidróxido de Cálcio/metabolismo , Membrana Celular/enzimologia , Cavidade Pulpar/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Radical Hidroxila/metabolismo , ÍonsRESUMO
Utilizando 40 pré-molares superiores e inferiores de cäes, os autores estudaram os efeitos de diferentes veículos para a pasta de hidróxido de cálcio no tratamento de perfuraçöes radiculares. Após os procedimentos endodônticos, realizou-se perfuraçöes na raiz mesial ao nivel e em direçäo à furca e os trajetos das perfuraçöes foram preenchidos com a pasta de hidróxido de cálcio e iodofórmio empregando-se como veículos, soro fisiológico, polietileno glicol e lipiodol U.F. O melhor resultado foi obtido quando se empregou o polietileno glicol como veículo para o hidróxido de cálcio