RESUMO
By employing an innovative biohybrid membrane, the present study aimed at elucidating the mechanistic role of the focal adhesion kinase (FAK) in epithelial morphogenesis in vitro over 4, 7, and 10 days. The consequences of siRNA-mediated FAK knockdown on epithelial morphogenesis were monitored by quantifying cell layers and detecting the expression of biomarkers of epithelial differentiation and homeostasis. Histologic examination of FAK-depleted samples showed a significant increase in cell layers resembling epithelial hyperplasia. Semiquantitative fluorescence imaging (SQFI) revealed tissue homeostatic disturbances by significantly increased involucrin expression over time, persistence of yes-associated protein (YAP) and an increase of keratin (K) 1 at day 4. The dysbalanced involucrin pattern was underscored by ROCK-IISer1366 activity at day 7 and 10. SQFI data were confirmed by quantitative PCR and Western blot analysis, thereby corroborating the FAK shutdown-related expression changes. The artificial FAK shutdown was also associated with a significantly higher expression of filaggrin at day 10, sustained keratinocyte proliferation, and the dysregulated expression of K19 and vimentin. These siRNA-induced consequences indicate the mechanistic role of FAK in epithelial morphogenesis by simultaneously considering prospective biomaterial-based epithelial regenerative approaches.
Assuntos
Proteínas de Ciclo Celular/genética , Células Epiteliais/citologia , Proteína-Tirosina Quinases de Adesão Focal/genética , Hiperplasia Epitelial Focal/genética , Morfogênese/genética , Fatores de Transcrição/genética , Biomarcadores/metabolismo , Células Epiteliais/metabolismo , Proteínas Filagrinas , Proteína-Tirosina Quinases de Adesão Focal/antagonistas & inibidores , Hiperplasia Epitelial Focal/patologia , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Regeneração Tecidual Guiada , Humanos , Proteínas de Filamentos Intermediários/genética , Queratina-1/genética , Queratinócitos/efeitos dos fármacos , Precursores de Proteínas/genética , RNA Interferente Pequeno/farmacologiaRESUMO
Germline mutations in BRAF are a major cause of cardio-facio-cutaneous (CFC) syndrome, which is characterized by heart defects, characteristic craniofacial dysmorphology and dermatologic abnormalities. Patients with CFC syndrome also commonly show gastrointestinal dysfunction, including feeding and swallowing difficulties and gastroesophageal reflux. We have previously found that knock-in mice expressing a Braf Q241R mutation exhibit CFC syndrome-related phenotypes, such as growth retardation, craniofacial dysmorphisms, congenital heart defects and learning deficits. However, it remains unclear whether BrafQ241R/+ mice exhibit gastrointestinal dysfunction. Here, we report that BrafQ241R/+ mice have neonatal feeding difficulties and esophageal dilation. The esophagus tissues from BrafQ241R/+ mice displayed incomplete replacement of smooth muscle with skeletal muscle and decreased contraction. Furthermore, the BrafQ241R/+ mice showed hyperkeratosis and a thickened muscle layer in the forestomach. Treatment with MEK inhibitors ameliorated the growth retardation, esophageal dilation, hyperkeratosis and thickened muscle layer in the forestomach in BrafQ241R/+ mice. The esophageal dilation with aberrant skeletal-smooth muscle boundary in BrafQ241R/+ mice were recovered after treatment with the histone H3K27 demethylase inhibitor GSK-J4. Our results provide clues to elucidate the pathogenesis and possible treatment of gastrointestinal dysfunction and failure to thrive in patients with CFC syndrome.
Assuntos
Displasia Ectodérmica/enzimologia , Estenose Esofágica/enzimologia , Insuficiência de Crescimento/enzimologia , Hiperplasia Epitelial Focal/enzimologia , Cardiopatias Congênitas/enzimologia , Proteínas Proto-Oncogênicas B-raf/genética , Proteínas Proto-Oncogênicas B-raf/metabolismo , Gastropatias/enzimologia , Animais , Displasia Ectodérmica/genética , Displasia Ectodérmica/patologia , Estenose Esofágica/genética , Estenose Esofágica/patologia , Fácies , Insuficiência de Crescimento/genética , Insuficiência de Crescimento/patologia , Feminino , Hiperplasia Epitelial Focal/genética , Mutação em Linhagem Germinativa , Cardiopatias Congênitas/genética , Cardiopatias Congênitas/patologia , MAP Quinase Quinase Quinases/antagonistas & inibidores , MAP Quinase Quinase Quinases/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Camundongos Transgênicos , Inibidores de Proteínas Quinases/farmacologia , Gastropatias/genéticaRESUMO
OBJECTIVES: We aimed to elucidate the molecular pathways associated with fluorescence properties of oral potentially malignant disorders (OPMD) visualised under direct tissue autofluorescence (VELscope(™)). MATERIALS AND METHODS: Forty-two oral mucosal biopsies correlated with clinical fluorescence characteristics were categorised based on histopathological diagnosis. Four oral squamous cell carcinoma (OSCC), 15 oral epithelial dysplasia (OED), nine oral lichen planus (OLP) and 14 oral epithelial hyperplasia (OEH) presenting with three fluorescence patterns including retained fluorescence (RF), loss of fluorescence (LAF) with blanching (LB) and LAF with no blanching (LNB) were assessed. Relative gene expression was measured through RNA sequencing. RESULTS: Although each lesion type had a specific set of histology-related differentially expressed genes (DEGs), all tested samples shared a number of DEGs, and we could not identify a discriminatory component between histological groups. Gene ontology enrichment revealed LAF in OEH was mostly due to changes in inflammation, cell cycle regulation and apoptosis, while in OED was due to inflammation, angiogenesis and extracellular matrix remodelling. Inflammatory reactions were associated with diascopic fluorescence (DF) for both OEH and OED. CONCLUSION: Uncovering the molecular mechanisms underlying LAF and DF may lead to reduction in the number of false-positive and false-negative findings and improve the efficacy and utility of VELscope(™).
Assuntos
Carcinoma de Células Escamosas/genética , Hiperplasia Epitelial Focal/genética , Expressão Gênica , Líquen Plano Bucal/genética , Neoplasias Bucais/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Apoptose/genética , Carcinoma de Células Escamosas/diagnóstico por imagem , Pontos de Checagem do Ciclo Celular/genética , Matriz Extracelular/metabolismo , Feminino , Hiperplasia Epitelial Focal/diagnóstico por imagem , Humanos , Inflamação/genética , Líquen Plano Bucal/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/diagnóstico por imagem , Neoplasias Bucais/diagnóstico por imagem , Neovascularização Patológica/genética , Imagem ÓpticaRESUMO
BACKGROUND: Focal epithelial hyperplasia (FEH) is a rare and benign papillomatous disease of the oral cavity, which is closely associated with human papillomavirus (HPV) type 13 and 32. Genetic susceptibility to HPV infections are supported by recent studies involving the human leukocyte antigen system (HLA). In this report, we aimed to determine the clinicopathological features of a Turkish family with FEH and to detect the shared HLA DR and DQ types. METHODS: HPV DNA typing of tissue samples and HLA determination from blood samples of four family members were performed by polymerase chain reaction. RESULTS: Histopathological examination of all patients revealed acanthotic papillomatous epidermis, koilocytes, apoptotic keratinocytes, and mitosoid bodies. HPV13 was detected by polymerase chain reaction. HLA DQA1*0501, HLA DQB1*0302, and HLA DRB1*11 alleles were common in all family members. HLA DRB1*04 was detected in three of them. CONCLUSION: This report is the first step for the investigation of involvement of HLA types in the pathogenesis of Turkish patients with FEH.
Assuntos
Hiperplasia Epitelial Focal/genética , Hiperplasia Epitelial Focal/patologia , Cadeias alfa de HLA-DQ/genética , Cadeias beta de HLA-DQ/genética , Adolescente , Adulto , Alelos , Apoptose , Criança , Feminino , Hiperplasia Epitelial Focal/virologia , Predisposição Genética para Doença/genética , Cadeias HLA-DRB1/genética , Humanos , Queratinócitos/citologia , Masculino , Papillomaviridae , Infecções por Papillomavirus/complicações , TurquiaRESUMO
Dermal infiltration of T cells is an important step in the onset and progression of immune-mediated skin diseases such as psoriasis; however, it is not known whether epidermal factors play a primary role in the development of these diseases. Here, we determined that the prodifferentiation transcription factor grainyhead-like 3 (GRHL3), which is essential during epidermal development, is dispensable for adult skin homeostasis, but required for barrier repair after adult epidermal injury. Consistent with activation of a GRHL3-regulated repair pathway in psoriasis, we found that GRHL3 is upregulated in lesional skin and binds known epidermal differentiation gene targets. Using an imiquimod-induced model of immune-mediated epidermal hyperplasia, we found that mice lacking GRHL3 have an exacerbated epidermal damage response, greater sensitivity to disease induction, delayed resolution of epidermal lesions, and resistance to anti-IL-22 therapy compared with WT animals. ChIP-Seq and gene expression profiling of murine skin revealed that while GRHL3 regulates differentiation pathways both during development and during repair from immune-mediated damage, it targets distinct sets of genes in the 2 processes. In particular, GRHL3 suppressed a number of alarmin and other proinflammatory genes after immune injury. This study identifies a GRHL3-regulated epidermal barrier repair pathway that suppresses disease initiation and helps resolve existing lesions in immune-mediated epidermal hyperplasia.
Assuntos
Reparo do DNA , Proteínas de Ligação a DNA/metabolismo , Epiderme/metabolismo , Hiperplasia Epitelial Focal/metabolismo , Linfócitos T Reguladores/metabolismo , Fatores de Transcrição/metabolismo , Animais , Diferenciação Celular/genética , Proteínas de Ligação a DNA/genética , Epiderme/patologia , Hiperplasia Epitelial Focal/genética , Hiperplasia Epitelial Focal/patologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/genética , Camundongos , Camundongos Knockout , Linfócitos T Reguladores/patologia , Fatores de Transcrição/genéticaRESUMO
Focal epithelial hyperplasia (FEH) is a benign proliferative condition that is more frequently found in children of certain ethnic groups. Human papillomavirus (HPV) 13 and 32 genotypes has been consistently detected in these lesions. In this study a daughter, mother and father had FEH, and HPV 13 was shown by sequence analysis in the lesions of these patients. Cryotherapy was applied to the lesions and the lesions improved, but did not recover properly. In conclusion, HPV genotyping should be performed in FEH cases.
Assuntos
Hiperplasia Epitelial Focal/virologia , Núcleo Familiar , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/complicações , Criança , Pai , Feminino , Hiperplasia Epitelial Focal/genética , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Mães , TurquiaRESUMO
Insufficiency of the transcriptional regulator GTF2IRD1 has become a strong potential explanation for some of the major characteristic features of the neurodevelopmental disorder Williams-Beuren syndrome (WBS). Genotype/phenotype correlations in humans indicate that the hemizygous loss of the GTF2IRD1 gene and an adjacent paralogue, GTF2I, play crucial roles in the neurocognitive and craniofacial aspects of the disease. In order to explore this genetic relationship in greater detail, we have generated a targeted Gtf2ird1 mutation in mice that blocks normal GTF2IRD1 protein production. Detailed analyses of homozygous null Gtf2ird1 mice have revealed a series of phenotypes that share some intriguing parallels with WBS. These include reduced body weight, a facial deformity resulting from localised epidermal hyperplasia, a motor coordination deficit, alterations in exploratory activity and, in response to specific stress-inducing stimuli; a novel audible vocalisation and increased serum corticosterone. Analysis of Gtf2ird1 expression patterns in the brain using a knock-in LacZ reporter and c-fos activity mapping illustrates the regions where these neurological abnormalities may originate. These data provide new mechanistic insight into the clinical genetic findings in WBS patients and indicate that insufficiency of GTF2IRD1 protein contributes to abnormalities of facial development, motor function and specific behavioural disorders that accompany this disease.
Assuntos
Hiperplasia Epitelial Focal/etiologia , Transtornos das Habilidades Motoras/etiologia , Proteínas Musculares/genética , Mutação/genética , Proteínas Nucleares/genética , Transativadores/genética , Vocalização Animal/fisiologia , Síndrome de Williams/complicações , Análise de Variância , Animais , Animais Recém-Nascidos/sangue , Temperatura Corporal/genética , Peso Corporal/genética , Encéfalo/metabolismo , Ritmo Circadiano/genética , Corticosterona/sangue , Modelos Animais de Doenças , Comportamento Exploratório/fisiologia , Gorduras , Feminino , Hiperplasia Epitelial Focal/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Transtornos das Habilidades Motoras/genética , Força Muscular , Músculo Esquelético/patologia , Fenótipo , Fatores Sexuais , Sono/genética , Espectrografia do Som , Estresse Psicológico/genética , Natação/psicologia , Síndrome de Williams/genética , Síndrome de Williams/patologiaRESUMO
Focal epithelial hyperplasia, or Heck's disease, is an uncommon proliferation of oral mucosa that presents primarily in Native Central and South American populations. It presents as asymptomatic papules or nodules on the oral mucosa, gingiva, tongue, and lips. In the majority of cases, human papilloma virus 13 or 32 is detected. Factors that determine disease susceptibility are unclear, but genetics, and having the human lymphocytic antigen-DR4 (DRB1*0404) allele in particular, are thought to play a major role in disease vulnerability. We report another case of focal epithelial hyperplasia, hypothesize on disease susceptibility, and review the current understanding of this uncommon disorder.
Assuntos
Hiperplasia Epitelial Focal/genética , Hiperplasia Epitelial Focal/patologia , Antígenos HLA-DR/genética , Mucosa Bucal/patologia , Biópsia , Criança , Feminino , Hiperplasia Epitelial Focal/virologia , Predisposição Genética para Doença , Cadeias HLA-DRB1 , Humanos , Lábio/patologia , Lábio/virologia , Mucosa Bucal/virologia , Papillomaviridae , Infecções Tumorais por Vírus/patologia , Vacúolos/patologiaRESUMO
La hiperplasia epitelial focal (HEF) o enfermedad de Heck es una afección poco frecuente de la mucosa oral producida por la asociación entre el virus del papiloma humano (HPV) subtipos 13 y 32 y un factor genético predisponente. Se inicia a edades tempranas, afecta a ambos sexos por igual y tiene preferencia por la población indígena. Clínicamente se caracteriza por múltiples pápulas circunscriptas o nódulos en la cavidad oral (mucosa labial, yugal y lingual), asintomáticas, de evolución variable y malignización extraordinaria. Se comunican ocho casos que fueron vistos en la Sección Estomatología de la División Dermatología de nuestro hospital.
Assuntos
Humanos , Adulto , Criança , Hiperplasia Epitelial Focal/genética , Hiperplasia Epitelial Focal/patologia , Hiperplasia Epitelial Focal/tratamento farmacológico , Lábio/patologia , Mucosa Bucal/patologia , Infecções por PapillomavirusRESUMO
Summary. Background. This report describes the case of a patient with focal epithelial hyperplasia (FEH), a rare but distinctive entity of viral aetiology with characteristic clinical and histopathological features. Case report. The condition is usually seen in children and adolescents of American Indian and Eskimo background. Surgical removal of papillomatous lesions is the treatment of choice, either for aesthetic reasons, or when the lesions interfere with function or are readily traumatized. Recurrence and the site of new lesions are unpredictable, and continued review of the patient is often necessary. The patient described here has been followed for 24 months without recurrences or changes in the aspect of the remaining lesions. Conclusion. This case highlights a possible genetic predilection for FEH, since the patient is a descent of a Brazilian Xavante Indian.
Assuntos
Hiperplasia Epitelial Focal/diagnóstico , Doenças da Gengiva/diagnóstico , Indígenas Sul-Americanos , Adolescente , Brasil , Feminino , Hiperplasia Epitelial Focal/genética , Seguimentos , Predisposição Genética para Doença , Doenças da Gengiva/genética , Humanos , Indígenas Sul-Americanos/genética , RecidivaRESUMO
C-cell hyperplasias are normally multifocal in multiple endocrine neoplasia type 2A. We compared clonality, microsatellite pattern of tumor suppressor genes, and cellular kinetics of C-cell hyperplasia foci in each thyroid lobe. We selected 11 females from multiple endocrine neoplasia type 2A kindred treated with thyroidectomy due to hypercalcitoninemia. C-cell hyperplasia foci were microdissected for DNA extraction to analyze the methylation pattern of androgen receptor alleles and microsatellite regions (TP53, RB1, WT1, and NF1). Consecutive sections were selected for MIB-1, pRB1, p53, Mdm-2, and p21WAF1 immunostaining, DNA content analysis, and in situ end labeling. Appropriate tissue controls were run. Only two patients had medullary thyroid carcinoma foci. Nine informative C-cell hyperplasia patients showed germline point mutation in RET, eight of them with the same androgen receptor allele preferentially methylated in both lobes. C-cell hyperplasia foci showed heterogeneous DNA deletions revealed by loss of heterozygosity of TP53 (12 of 20), RB1 (6 of 14), and WT1 (4 of 20) and hypodiploid G0/G1 cells (14 of 20), low cellular turnover (MIB-1 index 4.5%, in situ end labeling index 0.03%), and significantly high nuclear area to DNA index ratio. MEN 2A (germline point mutation in RET codon 634) C-cell hyperplasias are monoclonal and genetically heterogeneous and show down-regulated apoptosis, findings consistent with an intraepithelial neoplasia. Concordant X-chromosome inactivation and interstitial gene deletions suggest clone expansions of precursors occurring at a point in embryonic development before divergence of each thyroid lobe and may represent a paradigm for other germline mutations.
Assuntos
Proteínas de Drosophila , Mutação em Linhagem Germinativa , Neoplasia Endócrina Múltipla Tipo 2a/genética , Proteínas Proto-Oncogênicas/genética , Receptores Proteína Tirosina Quinases/genética , Substituição de Aminoácidos , Antígenos Nucleares , Autoantígenos/genética , Calcitonina/sangue , Carcinoma Medular/genética , Carcinoma Medular/cirurgia , Cisteína , Primers do DNA , Feminino , Hiperplasia Epitelial Focal/genética , Genes do Retinoblastoma , Humanos , Antígeno Ki-67 , Perda de Heterozigosidade , Neoplasia Endócrina Múltipla Tipo 2a/complicações , Neoplasia Endócrina Múltipla Tipo 2a/patologia , Proteínas do Tecido Nervoso/genética , Neurofibromina 1 , Proteínas Nucleares/genética , Polimorfismo de Nucleotídeo Único , Proteínas Proto-Oncogênicas c-ret , Doenças da Glândula Tireoide/complicações , Doenças da Glândula Tireoide/genética , Doenças da Glândula Tireoide/cirurgia , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia , TirosinaRESUMO
A 31-year-old Turkish patient and some family members suffered from multiple hyperplastic oral mucosal papules. Intralesional papilloma virus was not found but the patient had elevated levels of CD8 lymphocytes in his peripheral blood. We diagnosed focal epithelial hyperplasia of Heck.
Assuntos
Hiperplasia Epitelial Focal/genética , Mucosa Bucal/patologia , Adulto , Biópsia , Diagnóstico Diferencial , Hiperplasia Epitelial Focal/patologia , Alemanha , Humanos , Masculino , Linhagem , Turquia/etnologiaRESUMO
Os autores relatam um caso de hiperplasia epitelial focal em indivíduo de ascendência indígena e fazem revisäo da literatura pertinente