Efeito do Inibidor do ativador do plasminogênio 1 (PAI-1) nos parâmetros cinéticos de espermatozoides criopreservados de touros Curraleiro Pé Duro / Inhibitor effect of plasminogen activator 1 (PAI-1) in the kinetic parameters of sperm cryopreserved of Curraleiro Pé-Duro bulls

This study was conducted to evaluate the effect of different plasminogen activator inhibitor 1concentrations (70 ƞg, 140 and 210 ƞg ƞg) in the kinetic parameters of sperm cryopreserved of Curraleiro FootHard bulls. Sperm kinetics were analyzed by means of a computer aided system (CASA). The variables evaluatedwere: (MT-%) (MOP-um / s), (VCL - um / s), (VSL - um / s), (VAP-um / s), (LIN -%) ( Str-%) (ALH - uM)Wobble (WOB -%) and (BCF-Hz). The cryopreserved sperm in the presence of the inhibitor of plasminogenactivator 1 in a concentration of 210 ƞg decreased velocity parameters in a straight line (VSL - um / s) andlinearity (LIN -%). In conclusion, supplementation of the Inhibitor of plasminogen activator 1 (PAI-1),cryopreservation of bovine semen does not improve the kinetic parameters as compared to the control.(AU)

Efeito do Inibidor do ativador do plasminogênio 1 (PAI-1) nos parâmetros cinéticos de espermatozoides criopreservados de touros Curraleiro Pé Duro / Inhibitor effect of plasminogen activator 1 (PAI-1) in the kinetic parameters of sperm cryopreserved of Curraleiro Pé-Duro bulls

This study was conducted to evaluate the effect of different plasminogen activator inhibitor 1concentrations (70 ƞg, 140 and 210 ƞg ƞg) in the kinetic parameters of sperm cryopreserved of Curraleiro FootHard bulls. Sperm kinetics were analyzed by means of a computer aided system (CASA). The variables evaluatedwere: (MT-%) (MOP-um / s), (VCL - um / s), (VSL - um / s), (VAP-um / s), (LIN -%) ( Str-%) (ALH - uM)Wobble (WOB -%) and (BCF-Hz). The cryopreserved sperm in the presence of the inhibitor of plasminogenactivator 1 in a concentration of 210 ƞg decreased velocity parameters in a straight line (VSL - um / s) andlinearity (LIN -%). In conclusion, supplementation of the Inhibitor of plasminogen activator 1 (PAI-1),cryopreservation of bovine semen does not improve the kinetic parameters as compared to the control.

Association between the presence of a 38 kDa factor in the seminal plasma and inhibition of sperm motility in jundiá fish Rhamdia quelen / Associação entre a presença de um fator de 38 kDa no plasma seminal e a inibição da motilidade espermática no jundiá Rhamdia quelen

Fatores proteicos tem sido indentificados no plasma seminal de peixes e mamíferos e, em algumas situações, associados com indicadores de qualidade espermática. Entretanto, para o jundiá (Rhamdia quelen), tais fatores como aqueles com potenciais associações ainda não foram descritos. Os objetivos deste estudo foram de identificar alguns fatores proteicos presentes no plasma seminal do jundiá e avaliar suas associações com a motilidade espermática. Através de eletroforese do tipo SDS-PAGE foram identificadas 14 bandas proteicas com peso molecular entre 217.1 e 7.1 kDa. A motilidade espermática foi avaliada em 21 machos. Quatro bandas proteicas (81.5; 60.4; 33.6 e 25.5 kDa) foram detectadas em todas as amostras de plasma seminal analisadas. Uma banda proteica com peso molecular de 38.3 kDa foi associada com a baixa motilidade espermática no jundiá (P< 0,01), uma vez que foi detectada em 91.4% das amostras com motilidade menor que 80%. Estes resultados sugerem que esta banda proteica seminal associada com a baixa motilidade espermática poderá ser considerada como um potencial marcador bioquímico de qualidade seminal.

Protein factors have been identified in the seminal plasma of fish and mammal species and, in some situations, associated to sperm quality indicators. However, for jundiá fish (Rhamdia quelen), such factors and those potential associations remain unknown. In the present study, we aimed to identify some protein factors present in the seminal plasma of jundiá fish and to evaluate their association to sperm motility. SDS-PAGE was used to identify 14 bands, with molecular weight ranging from 217.1 to 7.1 kDa. Sperm motility was evaluated for 21 males. Four protein bands (81.5; 60.4; 33.6; and 25.5 kDa) were present in all seminal plasma samples. One protein band with molecular weight of 38.3 kDa was associated to reduced sperm motility of jundiá (P<0.01), since it was detected in 91.4% of the samples having motility lower than 80%. These results suggest that this seminal protein band associated to lower sperm motility may be considered a potential biochemical marker for sperm quality.

Association between the presence of a 38 kDa factor in the seminal plasma and inhibition of sperm motility in jundiá fish Rhamdia quelen / Associação entre a presença de um fator de 38 kDa no plasma seminal e a inibição da motilidade espermática no jundiá Rhamdia quelen

Fatores proteicos tem sido indentificados no plasma seminal de peixes e mamíferos e, em algumas situações, associados com indicadores de qualidade espermática. Entretanto, para o jundiá (Rhamdia quelen), tais fatores como aqueles com potenciais associações ainda não foram descritos. Os objetivos deste estudo foram de identificar alguns fatores proteicos presentes no plasma seminal do jundiá e avaliar suas associações com a motilidade espermática. Através de eletroforese do tipo SDS-PAGE foram identificadas 14 bandas proteicas com peso molecular entre 217.1 e 7.1 kDa. A motilidade espermática foi avaliada em 21 machos. Quatro bandas proteicas (81.5; 60.4; 33.6 e 25.5 kDa) foram detectadas em todas as amostras de plasma seminal analisadas. Uma banda proteica com peso molecular de 38.3 kDa foi associada com a baixa motilidade espermática no jundiá (P< 0,01), uma vez que foi detectada em 91.4% das amostras com motilidade menor que 80%. Estes resultados sugerem que esta banda proteica seminal associada com a baixa motilidade espermática poderá ser considerada como um potencial marcador bioquímico de qualidade seminal.(AU)

Protein factors have been identified in the seminal plasma of fish and mammal species and, in some situations, associated to sperm quality indicators. However, for jundiá fish (Rhamdia quelen), such factors and those potential associations remain unknown. In the present study, we aimed to identify some protein factors present in the seminal plasma of jundiá fish and to evaluate their association to sperm motility. SDS-PAGE was used to identify 14 bands, with molecular weight ranging from 217.1 to 7.1 kDa. Sperm motility was evaluated for 21 males. Four protein bands (81.5; 60.4; 33.6; and 25.5 kDa) were present in all seminal plasma samples. One protein band with molecular weight of 38.3 kDa was associated to reduced sperm motility of jundiá (P<0.01), since it was detected in 91.4% of the samples having motility lower than 80%. These results suggest that this seminal protein band associated to lower sperm motility may be considered a potential biochemical marker for sperm quality.(AU)

Sperm motility and viability in west African dwarf rams treated with Euphorbia hirta / Motilidad y viabilidad espermática en carneros enanos del oeste africano tratados con Euphorbia hirta

Four adult sexually matured and clinically healthy West African Dwarf (WAD) rams aged between 24 and 30 months were used for the study. The rams were first used as control and later as experimental animals upon being orally dosed with Euphorbia hirta extract at 400mg/kg body weight for 14 days. Semen samples were collected from the rams a day after the administration of the plant extra and seven days after. The objective of the study was to investigate the effect of Euphorbia hirta on the semen picture of WAD rams. There were significantly differences (P <0.05) in the semen picture as reflected in a reduction of sperm motility from 80% to 47.5% and live-dead ratio from 90.75% to 32.5% in the control and post-experimental stages of the study respectively. This indicates that the fertilization capacity and livability of spermatozoa were negatively affected. There were no significant differences in the values of body parameters measured across the stages of the study. The plant is therefore not recommended for medicinal purpose in male animals.

Cuatro carneros enanos adultos de África Occidental sexualmente maduros y clínicamente sanos, con edades comprendidas entre los 24 y 30 meses, fueron utilizados para este estudio. Los carneros fueron utilizados como control y, más tarde, como animales de experimentación al ser medicados por vía oral con extracto de Euphorbia hirta en 400mg/kg peso corporal durante 14 días. Se recogieron muestras de semen de los carneros un día después de la administración de la planta y siete días después. El objetivo del estudio fue investigar el efecto de Euphorbia hirta en las imágenes de esperma de carneros enanos África Occidental. Hubo diferencias significativas (P <0,05) en la imagen del semen como reflejo de una reducción de la motilidad espermática del 80% al 47,5% y un ratio de vivos-muertos de 90,75% a 32,5% en la etapa control y después de las fases experimentales del estudio, respectivamente. Esto indica que la capacidad de fertilización y calidad de vida de los espermatozoides fueron afectados negativamente. No hubo diferencias significativas en los valores de los parámetros corporales medidos a través de las etapas del estudio. La planta por tanto no es recomendable para fines medicinales en los animales machos.

Calcium (hydrogen-1-malate) hexahydrate on Echeveria gibbiflora leaves and its effect on sperm cells.

Echeveria gibbiflora is a plant widely used for its contraceptive activity in traditional Mexican medicine. Data on calcium crystals in plants are not outstanding. In the case of the Echeveria gibbiflora leaves, however, its quality, quantity, and salt type are quite surprising; one striking result of its X-ray crystallographic data shows the presence of calcium bis (hydrogen-1-malate) hexahydrate [2(C4H5O(5)1), Ca(1)2+, 6(H2O1)]. This highly soluble compound might explain the rapid shape changes of calcium crystals. Because SEM-EDS analysis shows that calcium malate crystals were obtained in a highly pure state and the immobilization and agglutination pattern that OBACE show on human and bull spermatozoa are not found even when high concentrations of calcium bis (hydrogen-1-malate) hexahydrate salt are present it is not feasible to involucrate molecules as calcium malate as part of the OBACE contraceptive activity.

Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm.

The ability of human follicular fluid (hFF), retrieved from women undergoing IVF to induce the acrosome reaction (AR) in human sperm has been documented by several laboratories. However, the nature of the active factors in the hFF and the physiological meaning of the AR induction are highly controversial. We performed a three step purification scheme for hFF and all the fractions were screened for the AR-inducing activity. AR activity was associated with a protein fraction of M(r) > 180 kD that on further analysis under PAGE was found to be composed by subunits of apparent M(r) 50,000 and 29,000. The N-terminal sequences of these bands showed a 100% homology with the heavy and light chains of human IgG. A polyclonal antibody raised against the purified protein and anti-human IgG were both able to suppress the acrosome reaction-inducing activity of crude hFF. However, neither normal human serum nor a purified preparation of human IgG were able to mimic the AR-inducing activity of hFF. We concluded that the AR-inducing activity of hFF is, at least in part, due to the presence of antisperm antibodies.

Inactivation of SH groups with sesquiterpene lactones: effects on nuclear decondensation pattern / motility induced by heparin in human spermatozoa.

PIP: In Guadalajara and Mexico City, Mexico, researchers collected and analyzed semen samples from fertile men who were sexually abstinent for 3 days to evaluate the effect heparin has on sperm nuclear decondensation patterns and motility. They used 3 sesquiterpene-lactones to inactivate the thiol groups on the outer membrane of sperm cells: I (17, 18 dehydroviguiepinin), II (Budlein A), and III (Zaluzanin A). Sesquiterpene-lactones I, II, an III had an inhibitory effect of 81%, 73%, and 27%, respectively, 6 hours after the sperm had been incubated with heparin for 6 hours. First mixing the sperm with reduced glutathione before adding 17, 18 dehydroviguiepinin increased the decondensating effect of heparin 350% above that of sperm not incubated with both glutathione and heparin. Sperm motility fell 80%, 60%, and 16%, respectively, 15 minutes after incubation with sesquiterpene-lactones I, II, and III. When sperm was incubated with heparin only, sperm motility was consistently higher for 5 hours. None of the compounds used to incubate the sperm affected sperm viability. The findings provided more information about the molecular biology of mammalian spermatozoa and suggested that sesquiterpene-lactones may be effective male contraceptives.^ieng