Assuntos
Vacina BCG , Lúpus Vulgar , Fator de Transcrição STAT1 , Humanos , Vacina BCG/efeitos adversos , Vacina BCG/administração & dosagem , Fator de Transcrição STAT1/genética , Lúpus Vulgar/diagnóstico , Lúpus Vulgar/genética , Lúpus Vulgar/tratamento farmacológico , Lúpus Vulgar/imunologia , Predisposição Genética para Doença , Masculino , Mycobacterium bovis/isolamento & purificação , Mycobacterium bovis/imunologia , FemininoRESUMO
Autoimmune-mediated congenital heart block (CHB) is a severe manifestation of neonatal lupus in which conduction tissues of the fetal heart are damaged. This occurs due to passive transference of maternal anti-SSA/Ro and anti-SSB/La autoantibodies and subsequent inflammation and fibrosis of the atrioventricular (AV) node. Notably, the disease manifests after the fetal heart has structurally developed, ruling out other anatomical abnormalities that could otherwise contribute to the block of conduction. Complete AV block is irreversible and the most common manifestation of CHB, although other cardiac complications such as endocardial fibroelastosis (EFE), dilated cardiomyopathy, and valvular insufficiency have been observed. In this review, we detail the classification, prevalence, pathogenesis, and clinical management recommendations for autoimmune CHB.
Assuntos
Autoanticorpos/imunologia , Autoantígenos/imunologia , Doenças Autoimunes/imunologia , Bloqueio Cardíaco/congênito , Lúpus Vulgar/imunologia , Complicações na Gravidez/imunologia , Adulto , Feminino , Coração Fetal , Bloqueio Cardíaco/diagnóstico , Bloqueio Cardíaco/imunologia , Humanos , GravidezAssuntos
Pavilhão Auricular/patologia , Lúpus Vulgar/diagnóstico , Pele/patologia , Adulto , Antituberculosos/uso terapêutico , Dermatomicoses/diagnóstico , Diagnóstico Diferencial , Quimioterapia Combinada , Pavilhão Auricular/imunologia , Pavilhão Auricular/microbiologia , Feminino , Humanos , Leishmaniose Cutânea/diagnóstico , Hanseníase/diagnóstico , Lúpus Vulgar/tratamento farmacológico , Lúpus Vulgar/imunologia , Lúpus Vulgar/microbiologia , Mycobacterium tuberculosis/imunologia , Mycobacterium tuberculosis/isolamento & purificação , Sarcoidose/diagnóstico , Pele/imunologia , Pele/microbiologia , Teste TuberculínicoAssuntos
Antituberculosos/uso terapêutico , Estética , Lúpus Vulgar/complicações , Deformidades Adquiridas Nasais/etiologia , Pele/patologia , Biópsia , Face , Feminino , Humanos , Lúpus Vulgar/diagnóstico , Lúpus Vulgar/tratamento farmacológico , Lúpus Vulgar/imunologia , Pessoa de Meia-Idade , Qualidade de VidaRESUMO
The NZM2410-derived Sle1a lupus susceptibility locus induces activated autoreactive CD4(+) T cells and reduces the number and function of Foxp3(+) regulatory T cells (Tregs). In this study, we first showed that Sle1a contributes to autoimmunity by increasing antinuclear antibody production when expressed on either NZB or NZW heterozygous genomes, and by enhancing the chronic graft versus host disease response indicating an expansion of the autoreactive B-cell pool. Screening two non-overlapping recombinants, the Sle1a.1 and Sle1a.2 intervals that cover the entire Sle1a locus, revealed that both Sle1a.1 and Sle1a.2 were necessary for the full Sle1a phenotype. Sle1a.1, and to a lesser extent Sle1a.2, significantly affected CD4(+) T-cell activation as well as Treg differentiation and function. Sle1a.2 also increased the production of autoreactive B cells. As the Sle1a.1 and Sle1a.2 intervals contain only 1 and 15 known genes, respectively, this study considerably reduces the number of candidate genes responsible for the production of autoreactive T cells. These results also show that the Sle1 locus is an excellent model for the genetic architecture of lupus, in which a major obligate phenotype results from the coexpression of multiple genetic variants with individual weak effects.
Assuntos
Predisposição Genética para Doença , Lúpus Eritematoso Sistêmico/genética , Linfócitos T/imunologia , Animais , Autoimunidade/genética , Autoimunidade/imunologia , Linfócitos B/imunologia , Linfócitos B/metabolismo , Diferenciação Celular/genética , Diferenciação Celular/imunologia , Feminino , Doença Enxerto-Hospedeiro/genética , Doença Enxerto-Hospedeiro/imunologia , Lúpus Eritematoso Discoide/genética , Lúpus Eritematoso Discoide/imunologia , Lúpus Eritematoso Sistêmico/imunologia , Lúpus Eritematoso Sistêmico/metabolismo , Lúpus Vulgar/genética , Lúpus Vulgar/imunologia , Ativação Linfocitária/genética , Ativação Linfocitária/imunologia , Camundongos , Camundongos Congênicos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NZB , Camundongos Endogâmicos , Camundongos Knockout , Linfócitos T/metabolismo , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/metabolismoRESUMO
Desmosomal cadherins are essential cell adhesion molecules expressed in the epidermis. We identified a mutation of a cadherin superfamily member, namely, desmoglein 4 (Dsg4), in early onset of death (EOD)( hage ) mice with hypotrichosis. The mutation was induced by the insertion of an early transposon II-beta into intron 8 of Dsg4. Mast cell hyperplasia was observed in the skin of EOD( hage ) mice. The abnormally expanded population of lpr T cells, i.e., CD4(-)CD8(-)B220(+)Thy1.2(+) alphabetaT cells, in the splenocytes of EOD mice was reduced in EOD( hage ) mice. Therefore, it was suspected that the long-living mutant EOD( hage ) mice were selected from lupus-prone EOD mice because of their immunological immaturity. These findings clearly indicate that Dsg4 is an important molecule for the formation of hair follicles and hypothesize that unorganized hyperplastic hair follicles in anagen due to the Dsg4 mutation provide niches for mast cell precursors in the skin.
Assuntos
Desmogleínas/fisiologia , Hipotricose/patologia , Lúpus Vulgar/patologia , Mastócitos/patologia , Mutação/genética , Pele/patologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Citometria de Fluxo , Predisposição Genética para Doença , Folículo Piloso/imunologia , Folículo Piloso/patologia , Hiperplasia , Hipotricose/genética , Hipotricose/imunologia , Íntrons/genética , Lúpus Vulgar/imunologia , Camundongos , Camundongos Endogâmicos MRL lpr/genética , Camundongos Knockout , Camundongos Mutantes/genética , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Pele/imunologia , Pele/metabolismo , Taxa de SobrevidaRESUMO
Durante los últimos años, se ha acumulado evidencia quedemuestra que la regulación del ciclo celular de las células es esencialpara establecer tolerancia y suprimir autoinmunidad. Aunquela apoptosis se ha considerado un mecanismo importante enel control del desarrollo de tolerancia y autoinmunidad, la regulacióndel ciclo celular también constituye una vía alternativaen la prevención de la autoreactividad. Diferentes moléculas asociadasal ciclo celular actúan como supresoras de la autoinmunidad.Se ha demostrado recientemente que los inhibidores delciclo celular p21 y p27 controlan la tolerancia de las células T,mientras que p21 también limita el desarrollo de la autoinmunidad.En esta revisión exploraremos los efectos de p21 y p27 enla inducción de la tolerancia de las células T y discutiremos la asociaciónentre pérdida de tolerancia y desarrollo de autoinmunidaden ratones p21/
Over the last few years, evidence has accumulated showingthat cell cycle regulation of T cells is essential to establish toleranceand to suppress autoimmunity. Although apoptosis hasbeen considered an important mechanism in the control of toleranceand autoimmunity development, cell cycle regulation alsoconstitutes a pivotal alternative pathway in the prevention of autoreactivity.Several cell cycle-associated molecules act as autoimmunitysuppressors. The cell cycle inhibitors p21 and p27 haverecently been shown to control T cell tolerance, while p21 also restrainsdevelopment of autoimmunity. In this review, we willexplore the effects of p21 and p27 in T cell tolerance induction anddiscuss the association between tolerance loss and autoimmunitydevelopment in p21/ mice (AU)
Assuntos
Humanos , Ciclo Celular/fisiologia , Autoimunidade/fisiologia , Homeostase/fisiologia , Apoptose/fisiologia , Linfócitos T/fisiologia , Produtos do Gene rex/fisiologia , Proteínas Proto-Oncogênicas p21(ras)/fisiologia , Lúpus Vulgar/imunologiaRESUMO
Congenital heart block (CHB) is the main complication of neonatal lupus (NL) and is strongly associated with the presence of anti-SSA/Ro and anti-SSB/La antibodies. The recurrence of CHB in subsequent pregnancies in mothers with these antibodies is uncommon, occurring in approximately 15% of cases. We describe here a case of recurrent CHB in a previously asymptomatic mother with Sjögren syndrome and discuss the current strategies for the prevention and treatment of CHB in NL.
Assuntos
Bloqueio Cardíaco/congênito , Bloqueio Cardíaco/imunologia , Lúpus Vulgar/imunologia , Complicações na Gravidez/imunologia , Adulto , Anticorpos Antinucleares/imunologia , Feminino , Humanos , Recém-Nascido , Lúpus Vulgar/patologia , Masculino , Gravidez , Recidiva , Síndrome de SjogrenRESUMO
Although systemic lupus erythematosus (SLE) is usually evaluated with regard to autoimmune reactivity toward the kidney, there are multiple psychiatric abnormalities associated with this autoimmune disease. Lupus-prone male NZM88 mice, derived from NZB/NZW F1 mice, develop early neuropsychiatric manifestations without any signs of nephritis. In addition to the usual repertoire of antibody specificities, including autoantibodies to dsDNA and renal antigens, mice of this inbred strain express autoantibodies to numerous brain antigens. Here, we show that autoantibodies to brain antigens, assessed by Western analysis, are as individually varied as are the diverse neuropsychiatric manifestations observed in SLE patients. Additionally, a monoclonal antibody derived from the spleen of an untreated NZM88 male when injected into healthy BALB/cByJ, but not C57BL/6J, mice induced behaviors similar to those of lupus-prone NZM88 mice. This monoclonal antibody, which is specific to dynamin-1, binds preferentially in BALB/cByJ cortex and induces substantial expression of cytokines mainly in the hypothalamus. Thus, an antibody to just one brain antigen can induce multiple behavioral changes, and multiple autoantibodies to different brain antigens exist in lupus-prone mice; however, susceptibility to the induction of neurobehavioral deficits is dependent on host genetics.
Assuntos
Anticorpos Monoclonais/imunologia , Comportamento Animal , Lúpus Vulgar/complicações , Lúpus Vulgar/imunologia , Transtornos Mentais/etiologia , Doenças do Sistema Nervoso/etiologia , Animais , Autoanticorpos/sangue , Autoantígenos/imunologia , Western Blotting , Encéfalo/imunologia , Encéfalo/metabolismo , Citocinas/biossíntese , Dinamina I/imunologia , Feminino , Predisposição Genética para Doença , Hipotálamo/metabolismo , Lúpus Vulgar/genética , Lúpus Vulgar/psicologia , Masculino , Transtornos Mentais/genética , Camundongos , Camundongos Endogâmicos/genética , Camundongos Mutantes , Doenças do Sistema Nervoso/genética , Especificidade da Espécie , Baço/imunologiaRESUMO
Although recent studies indicated that IL-21 is an important regulator of human B cell activation, detailed comparison of the effects of IL-21 on distinct B cell subsets have not been performed. Our studies revealed that IL-21R is expressed by naive and germinal center B cells, but not memory or plasma cells. IL-21R was increased on naive and memory B cells following in vitro activation. Investigation into the kinetics and magnitude of responses of human B cells to IL-21 revealed that IL-21 potently augmented proliferation of CD40L-stimulated neonatal, splenic naive, and memory and tonsil germinal center B cells. This response exceeded that induced by IL-4, IL-10, and IL-13, cytokines that also induce B cell proliferation. Remarkably, CD40L/IL-21-stimulated naive B cells underwent the same number of divisions as memory cells and exhibited a greater enhancement in their response compared with CD40L alone than memory B cells. Therefore, IL-21 is a powerful growth factor for naive B cells. This may result from the higher expression of IL-21R on naive, compared with memory, B cells. Stimulation of human B cells with CD40L/IL-21 also induced IL-10 production and activation of STAT3. We propose that IL-21 may have therapeutic application in conditions of immunodeficiency where it could expand naive B cells, the predominant B cell subset in such patients. Conversely, because IL-21 is increased in murine models of lupus, dysregulated IL-21 production may contribute to perturbed B cell homeostasis observed in systemic lupus erythematosus. Thus, antagonizing IL-21 may be a novel strategy for treating Ab-mediated autoimmune diseases.
Assuntos
Linfócitos B/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Interleucinas/farmacologia , Linfócitos B/química , Ligante de CD40/fisiologia , Citocinas/farmacologia , Centro Germinativo/citologia , Homeostase/imunologia , Humanos , Memória Imunológica , Cinética , Lúpus Vulgar/etiologia , Lúpus Vulgar/imunologia , Ativação Linfocitária/efeitos dos fármacos , Subpopulações de Linfócitos/química , Subpopulações de Linfócitos/efeitos dos fármacos , Receptores de Interleucina-21/análiseRESUMO
Systemic lupus erythematosus is an autoimmune disease characterized by autoantibodies and systemic clinical manifestations. A peptide, designated hCDR1, based on the complementarity-determining region (CDR) 1 of an autoantibody, ameliorated the serological and clinical manifestations of lupus in both spontaneous and induced murine models of lupus. The objectives of the present study were to determine the mechanism(s) underlying the beneficial effects induced by hCDR1. Adoptive transfer of hCDR1-treated cells to systemic lupus erythematosus-afflicted (NZBxNZW)F1 female mice down-regulated all disease manifestations. hCDR1 treatment up-regulated (by 30-40%) CD4+CD25+ cells in association with CD45RBlow, cytotoxic T lymphocyte antigen 4, and Foxp3 expression. Depletion of the CD25+ cells diminished significantly the therapeutic effects of hCDR1, whereas administration of the enriched CD4+CD25+ cell population was beneficial to the diseased mice. Amelioration of disease manifestations was associated with down-regulation of the pathogenic cytokines (e.g., IFN-gamma and IL-10) and up-regulation of the immunosuppressive cytokine TGF-beta, which substantially contributed to the suppressed autoreactivity. TGF-beta was secreted by CD4+ cells that were affected by hCDR1-induced immunoregulatory cells. The hCDR1-induced CD4+CD25+ cells suppressed autoreactive CD4+ cells, resulting in reduced rates of activation-induced apoptosis. Thus, hCDR1 ameliorates lupus through the induction of CD4+CD25+ cells that suppress activation of the autoreactive cells and trigger the up-regulation of TGF-beta.
Assuntos
Autoanticorpos/imunologia , Linfócitos T CD4-Positivos/imunologia , Regiões Determinantes de Complementaridade/imunologia , Lúpus Vulgar/imunologia , Peptídeos/imunologia , Receptores de Interleucina-2/imunologia , Fator de Crescimento Transformador beta/genética , Animais , Autoanticorpos/química , Linfócitos T CD4-Positivos/citologia , Linfócitos T CD4-Positivos/metabolismo , Morte Celular , Regiões Determinantes de Complementaridade/química , Feminino , Humanos , Imunoterapia Adotiva , Lúpus Vulgar/tratamento farmacológico , Ativação Linfocitária/imunologia , Camundongos , Peptídeos/química , Baço/citologia , Fator de Crescimento Transformador beta/metabolismo , Regulação para CimaRESUMO
Antibodies to dsDNA represent a classification criterion for systemic lupus erythematosus. Subpopulations of these antibodies are involved in lupus nephritis. No known marker separates nephritogenic from non-nephritogenic anti-dsDNA antibodies. It is not clear whether specificity for glomerular target antigens or intrinsic antibody-affinity for dsDNA or nucleosomes is a critical parameter. Furthermore, it is still controversial whether glomerular target antigen(s) is constituted by nucleosomes or by non-nucleosomal glomerular structures. Previously, we have demonstrated that antibodies eluted from murine nephritic kidneys recognize nucleosomes, but not other glomerular antigens. In this study, we determined the structures that bind nephritogenic autoantibodies in vivo by transmission electron microscopy, immune electron microscopy, and colocalization immune electron microscopy using experimental antibodies to dsDNA, to histones and transcription factors, or to laminin. The data obtained are consistent and point at glomerular basement membrane-associated nucleosomes as target structures for the nephritogenic autoantibodies. Terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling or caspase-3 assays demonstrate that lupus nephritis is linked to intraglomerular cell apoptosis. The data suggest that nucleosomes are released by apoptosis and associate with glomerulus basement membranes, which may then be targeted by pathogenic anti-nucleosome antibodies. Thus, apoptotic nucleosomes may represent both inducer and target structures for nephritogenic autoantibodies in systemic lupus erythematosus.
Assuntos
Apoptose , Membrana Basal/química , Glomérulos Renais/metabolismo , Lúpus Vulgar/imunologia , Nefrite/metabolismo , Animais , Autoanticorpos/química , Membrana Basal/embriologia , Bovinos , Histonas/metabolismo , Imunoglobulina G/química , Rim/metabolismo , Lúpus Vulgar/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Fatores de TempoRESUMO
Antibodies against citrullinated proteins are specific and predictive markers for rheumatoid arthritis although the pathologic relevance of these antibodies remains unclear. To investigate the significance of these autoantibodies, collagen-induced arthritis (CIA) in mice was used to establish an animal model of antibody reactivity to citrullinated proteins. DBA/1J mice were immunized with bovine type II collagen (CII) at days 0 and 21, and serum was collected every 7 days for analysis. Antibodies against both CII and cyclic citrullinated peptide, one such citrullinated antigen, appeared early after immunization, before joint swelling was observed. Further, these antibodies demonstrated specific binding to citrullinated filaggrin in rat esophagus by indirect immunofluorescence and citrullinated fibrinogen by Western blot. To evaluate the role of immune responses to citrullinated proteins in CIA, mice were tolerized with a citrulline-containing peptide, followed by antigen challenge with CII. Tolerized mice demonstrated significantly reduced disease severity and incidence compared with controls. We also identified novel murine monoclonal antibodies specific to citrullinated fibrinogen that enhanced arthritis when coadministered with a submaximal dose of anti-CII antibodies and bound targets within the inflamed synovium of mice with CIA. These results demonstrate that antibodies against citrullinated proteins are centrally involved in the pathogenesis of autoimmune arthritis.
Assuntos
Anticorpos Monoclonais/imunologia , Artrite Experimental/imunologia , Artrite Reumatoide/imunologia , Autoanticorpos/biossíntese , Peptídeos Cíclicos/imunologia , Animais , Anticorpos Monoclonais/metabolismo , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Artrite Experimental/microbiologia , Artrite Reumatoide/metabolismo , Bovinos , Colágeno , Fibrinogênio/imunologia , Fibrinogênio/metabolismo , Fibrinogênio/farmacologia , Lúpus Vulgar/imunologia , Lúpus Vulgar/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos DBA , Membrana Sinovial/imunologia , Membrana Sinovial/metabolismo , Membrana Sinovial/patologia , Fatores de TempoRESUMO
Inducible costimulator (ICOS) is a costimulatory molecule expressed in activated T cells and plays an important role in T-cell-dependent immune responses. We investigated the role of ICOS in the development of autoimmune diseases in MRL/Mpj-lpr/lpr (MRL/lpr) mice. ICOS was expressed on CD4(+) T cells from adult MRL/lpr mice. ICOS-deficient MRL/lpr mice showed mild lymphoadenopathy and a decreased memory type CD4(+) T cells in the spleen. The anti-dsDNA antibody levels were decreased. CD4(+) T cells from ICOS-deficient MRL/lpr mice showed less of a bias to Th1 and an enhanced production of IL-4 in response to anti-CD3 antibody in comparison to those from wild-type MRL/lpr mice. Although ICOS-deficiency abrogated renal vasculitis completely, the severity of glomerulonephritis was not altered. ICOS is considered to play a role in CD4(+) T cell activation, autoantibody production, and renal vasculitis. However, it is not essentially required in the development of glomerulonephritis.
Assuntos
Antígenos de Diferenciação de Linfócitos T/genética , Antígenos de Diferenciação de Linfócitos T/fisiologia , Lúpus Vulgar/etiologia , Animais , Citocinas/biossíntese , Citocinas/sangue , Modelos Animais de Doenças , Feminino , Glomerulonefrite/complicações , Glomerulonefrite/mortalidade , Imunoglobulinas/sangue , Proteína Coestimuladora de Linfócitos T Induzíveis , Rim/patologia , Lúpus Vulgar/complicações , Lúpus Vulgar/imunologia , Doenças Linfáticas/imunologia , Camundongos , Camundongos Endogâmicos MRL lpr , Camundongos Knockout , Baço/citologia , Baço/imunologia , Esplenomegalia/imunologia , Linfócitos T/imunologiaRESUMO
We analyzed the activation and function of dendritic cells (DCs) in the spleens of diseased, lupus-prone NZM2410 and NZB-W/F1 mice and age-matched BALB/c and C57BL/6 control mice. Lupus DCs showed an altered ex vivo costimulatory profile, with a significant increase in the expression of CD40, decreased expression of CD80 and CD54, and normal expression of CD86. DCs from young lupus-prone NZM2410 mice, before the development of the disease, expressed normal levels of CD80 and CD86 but already overexpressed CD40. The increase in CD40-positive cells was specific for DCs and involved the subset of myeloid and CD8alpha+ DCs before disease onset, with a small involvement of plasmacytoid DCs in diseased mice. In vitro data from bone marrow-derived DCs and splenic myeloid DCs suggest that the overexpression of CD40 is not due to a primary alteration of CD40 regulation in DCs but rather to an extrinsic stimulus. Our analyses suggest that the defect of CD80 in NZM2410 and NZB-W/F1 mice, which closely resembles the costimulatory defect found in DCs from humans with systemic lupus erythematosus, is linked to the autoimmune disease. The increase in CD40 may instead participate in disease pathogenesis, being present months before any sign of autoimmunity, and its downregulation should be explored as an alternative to treatment with anti-CD40 ligand in lupus.
Assuntos
Células Dendríticas , Lúpus Vulgar/patologia , Lúpus Vulgar/fisiopatologia , Fenótipo , Baço/patologia , Envelhecimento/imunologia , Animais , Antígeno B7-1/metabolismo , Antígeno B7-2/metabolismo , Antígenos CD40/metabolismo , Células Dendríticas/imunologia , Suscetibilidade a Doenças , Lúpus Vulgar/imunologia , Camundongos , Camundongos Endogâmicos , Índice de Gravidade de Doença , Baço/imunologiaRESUMO
The SmD1(83-119) peptide is a main target of autoantibodies and T cells in human and murine lupus, but its role in autoimmunity induction remains elusive. Therefore, female Balb/c mice and (NZW x Balb/c)F1 [CWF1] mice with identical MHC haplotype as lupus prone NZB/W mice were immunized with SmD1(83-119). Immunizations of CWF1 mice with SmD1(83-119), but not with the controls (irrelevant peptide, HEL peptide, or saline), induced anti-SmD1(83-119) and anti-dsDNA antibodies and proteinuria not present in Balb/c mice. DsDNA-specific plasma cell induction after SmD1(83-119) immunizations was confirmed by ELISPOT assays showing that the generation of dsDNA-specific antibody forming cells (AFC) was mainly driven by increased T-cell help. T-cell help for the generation of dsDNA-specific AFC was also present in saline-treated CWF1 mice but was controlled on the levels of B cells preventing autoimmunity.
Assuntos
Anticorpos Antinucleares/biossíntese , Linfócitos B/imunologia , DNA/imunologia , Lúpus Vulgar/genética , Ribonucleoproteínas Nucleares Pequenas/imunologia , Animais , Feminino , Imunização , Lúpus Vulgar/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NZB , Fragmentos de Peptídeos/imunologia , Linfócitos T/imunologia , Proteínas Centrais de snRNPRESUMO
An otherwise healthy 5-week-old infant with erythematous plaques predominantly on the face and scalp presented to our dermatology clinic. The mother had been diagnosed with lupus erythematosus 2 years earlier but her disease was quiescent. Neonatal lupus is a rare condition associated with transplacental transfer of IgG anti-SSA/Ro and anti-SSB/La antibodies from the mother to the fetus. Active connective tissue disease in the mother does not have to be present and in fact is often absent. Although the cutaneous, hematologic and hepatic manifestations are transient, the potential for permanent heart block makes it necessary for this to be carefully ruled out. As in this case, the dermatologist may be the one to make the diagnosis and should be aware of the clinical presentation, work-up, and management of this important disease.
Assuntos
Lúpus Vulgar/diagnóstico , Lúpus Vulgar/imunologia , Autoanticorpos/sangue , Autoanticorpos/imunologia , Dermatoses Faciais/imunologia , Feminino , Humanos , Lactente , Transmissão Vertical de Doenças Infecciosas , Lúpus Vulgar/complicações , Lúpus Vulgar/transmissãoRESUMO
A single intraperitoneal (i.p.) injection of pristane, incomplete Freund's adjuvant (IFA), or the adjuvant oil squalene, but not high molecular weight medicinal mineral oils, induces lupus-related autoantibodies to nRNP/Sm and -Su in non-autoimmune strains of mice. This ability appears to be associated with the low molecular weight and adjuvanticity of hydrocarbon. n-Hexadecane (C(16)H(34)), which is present in petroleum, has adjuvant activity and induces arthritis in rodents like other lupus-inducing oils. In addition to dietary exposure to n-hexadecane in mineral oils, exposure also occurs via inhalation of oil mist, jet fuel, or diesel exhaust or by absorption through the skin. Since n-hexadecane is a low molecular weight adjuvant hydrocarbon oil similar to other lupus-inducing hydrocarbons, the present study examined whether it can also induce lupus-related autoantibodies in mice. Female BALB/cJ mice received a single i.p. injection of 0.5 ml of n-hexadecane, pristane, or saline (control). Pathology and serology (immunoglobulin levels, autoantibodies by immunofluorescence, immunoprecipitation, and ELISA) were examined 3 months later. Unexpectedly, all n-hexadecane-treated mice, but none in the other groups, developed inflammatory ascites within 2.5 months. n-Hexadecane induced hypergammaglobulinemia (IgG1, IgG2a), antinuclear (titer>1:160, 67%) and -cytoplasmic antibodies (58%) and autoantibodies to nRNP/Sm (25%), Su (33%), ssDNA (83%), and chromatin (100%). Therefore, non-specific inflammation caused by n-hexadecane resulted in the production of a limited set of specific autoantibodies. These previously unrecognized immunological effects of n-hexadecane may have implications in monitoring human exposure to hydrocarbons and in the pathogenesis of autoimmune diseases.
Assuntos
Alcanos/toxicidade , Autoanticorpos/imunologia , Lúpus Vulgar/induzido quimicamente , Animais , Ascite/induzido quimicamente , Autoimunidade , Poluentes Ambientais/toxicidade , Feminino , Injeções Intraperitoneais , Fígado/efeitos dos fármacos , Fígado/patologia , Lúpus Vulgar/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Peritonite/induzido quimicamente , Baço/efeitos dos fármacos , Baço/patologiaRESUMO
To study central tolerance to the major product of ongoing apoptosis in the thymus, we made new lines of transgenic (Tg) mice expressing TCR of a pathogenic autoantibody-inducing Th cell that was specific for nucleosomes and its histone peptide H4(71-94). In the lupus-prone (SWR x NZB)F1 (SNF1) thymus, introduction of the lupus TCR transgene caused no deletion, but marked down-regulation of the Tg TCR and up-regulation of endogenous TCRs. Paradoxically, autoimmune disease was suppressed in the alphabetaTCR Tg SNF1 mice with induction of highly potent regulatory T cells in the periphery. By contrast, in the MHC-matched, normal (SWR x B10. D2)F1 (SBF1), or in the normal SWR backgrounds, marked deletion of transgenic thymocytes occurred. Thymic lymphoid cells of the normal or lupus-prone mice were equally susceptible to deletion by anti-CD3 Ab or irradiation. However, in the steady state, spontaneous presentation of naturally processed peptides related to the nucleosomal autoepitope was markedly greater by thymic dendritic cells (DC) from normal mice than that from lupus mice. Unmanipulated thymic DC of SNF1 mice expressed lesser amounts of MHC class II and costimulatory molecules than their normal counterparts. These results indicate that apoptotic nucleosomal autoepitopes are naturally processed and presented to developing thymocytes, and a relative deficiency in the natural display of nucleosomal autoepitopes by thymic DC occurs in lupus-prone SNF1 mice.