Regulation of UDPG-pyrophosphorylase isoforms in Saccharomyces cerevisiae and their roles in trehalose metabolism.

UDPG-pyrophosphorylase (EC 2.7.7.9) from Saccharomyces cerevisiae was studied and the presence of isoforms investigated. Its activity was monitored during growth of cultures in rich media containing glucose, galactose, sucrose, maltose or glycerol as carbon sources. The results suggest that UDPG-pyrophosphorylase is subject to both catabolite repression and catabolite inactivation. The inactivation process seems to be complex: in order to produce maximum inactivation, glucose and ammonium sulfate must be added together. Addition of glucose or ammonium sulfate separately produced little effect upon enzyme activity. Adsorption to and elution from a DEAE-Sephacel column of a crude protein extract prepared from yeast cells collected in stationary phase from a glucose medium showed three activity peaks, which we denominated isoform I, II, and III. Isoform I is constitutive, it was the only form present during exponential growth on glucose medium, and did not suffer any alteration after glucose exhaustion, heat shock or by growing cells on maltose. On the other hand, isoforms II and III were shown to be repressed by glucose, and induced by heat shock. Furthermore, isoform II of UDPG-pyrophosphorylase was present together with isoform I when yeast cells were grown on maltose. The presence of a MAL4C allele rendered isoform II constitutive. Interestingly, a gal3 mutant strain had low UDPG-pyrophosphorylase activity and isoforms I and II were not expressed. These results are discussed in relation to trehalose metabolism.

Recombination of lac- mal+ cells with lac- mal- variants arisen spontaneously from different Candida albicans strains

Cepas de Candida albicans, mantidas em meios de cultura usuais, originaram colônias que em testes de assimilaçäo de fontes de carbono mostraram-se lac+ mal-. Esferosplastos obtidos a partir de células normais, lac- mal+, foram submetidos à fusäo e produziram apenas células lac+ mal+. Após reduçäo na ploidia,essas células deram origem a colônias lac- mal+ (82 por cento), lac- mal (13,3 por cento) e lac+ mal+ (4,4 por cento). Discute-se neste trabalho, ploidia dos produtos de fusäo, ocorrência de heterocariose nesses produtos e a perda de cromossomos durante a segregaçäo