RESUMO
A new extraction method for the acetylgestagens medroxyprogesterone acetate (MPA), chloromadinone acetate and megestrol acetate, from kidney fat, has been developed. The method is a combination of matrix solid phase dispersion and solid phase extraction and is simpler and safer than previous methods, especially as it can be automated. The recovery was estimated as 59 +/- 5% (mean +/- standard deviation) for MPA. For screening purposes detection can be achieved using a commercially available enzyme immunoassay kit giving detection limits in the range of 1.0-2.0 ng g-1.
Assuntos
Tecido Adiposo/química , Acetato de Clormadinona/isolamento & purificação , Acetato de Medroxiprogesterona/isolamento & purificação , Megestrol/análogos & derivados , Matadouros , Automação/métodos , Acetato de Clormadinona/análise , Cromatografia Líquida de Alta Pressão/métodos , Técnicas Imunoenzimáticas , Rim , Acetato de Medroxiprogesterona/análise , Megestrol/análise , Megestrol/isolamento & purificação , Acetato de Megestrol , Técnica de Diluição de Radioisótopos , Kit de Reagentes para Diagnóstico , TrítioRESUMO
The optimization of the reversed phase high performance liquid chromatographic separation of six steroids of long acting contraceptives is described. A procedure is used to determine the design space, calculate the coefficients of a seven-term special cubic equation, and modify the model using the optimum experiment. Assisted with the computer, an optimum area could be predicted by overlapping the minimum resolution map with the analysis time map. It is likely that the method described in this paper will provide the basis or the simultaneous optimization of both separation and analysis time and prove to be a useful tool for the optimization of RP-HPLC of known compounds. We firstly utilized the time programming function to improve the detection sensitivities of estrogenic compounds. This method is successfully applied to the analysis of compound megestrol acetate injection and compound hydroxyprogesterone caproate injection. The method is sufficiently simple and rapid yet sensitive and accurate enough for the quality assurance of these types of pharmaceuticals.
Assuntos
Anticoncepcionais Femininos/química , Estradiol/análise , Cromatografia Líquida de Alta Pressão/métodos , Preparações de Ação Retardada , Estradiol/análogos & derivados , Hidroxiprogesteronas/análise , Megestrol/análogos & derivados , Megestrol/análise , Acetato de MegestrolRESUMO
The paper reports the simultaneous determination of megestrol acetate and estradiol valerate in the injection of microencapsulated compound megestrol acetate by secondary derivative spectrophotometry. The experimental results showed that the average recoveries of the two drugs were 98.8% and 98.0%, respectively, and that the coefficients of variation were 0.71% and 1.81%, respectively.
