RESUMO
Three indole alkaloids that possess differing degrees of psychotropic/psychedelic activity have been reported as endogenous substances in humans; N,N-dimethyltryptamine (DMT), 5-hydroxy-DMT (bufotenine, HDMT), and 5-methoxy-DMT (MDMT). We have undertaken a critical review of 69 published studies reporting the detection or detection and quantitation of these compounds in human body fluids. In reviewing this literature, we address the methods applied and the criteria used in the determination of the presence of DMT, MDMT, and HDMT. The review provides a historical perspective of the research conducted from 1955 to 2010, summarizing the findings for the individual compounds in blood, urine, and/or cerebrospinal fluid. A critique of the data is offered that addresses the strengths and weaknesses of the methods and approaches to date. The review also discusses the shortcomings of the existing data in light of more recent findings and how these may be overcome. Suggestions for the future directions of endogenous psychedelics research are offered.
Assuntos
Alucinógenos/sangue , Alucinógenos/urina , N,N-Dimetiltriptamina/sangue , N,N-Dimetiltriptamina/urina , Bufotenina/sangue , Bufotenina/líquido cefalorraquidiano , Bufotenina/história , Bufotenina/urina , Alucinógenos/líquido cefalorraquidiano , Alucinógenos/história , História do Século XX , História do Século XXI , Humanos , Metoxidimetiltriptaminas/sangue , Metoxidimetiltriptaminas/líquido cefalorraquidiano , Metoxidimetiltriptaminas/história , Metoxidimetiltriptaminas/urina , N,N-Dimetiltriptamina/líquido cefalorraquidiano , N,N-Dimetiltriptamina/históriaRESUMO
A new method for the determination of N,N-dimethyl-5-hydroxytryptamine (bufotenine), N,N-dimethyltryptamine (DMT)*, 5-methoxy-N,N-dimethyltryptamine (5-MeODMT), and N-methyltryptamine (NMT) was developed using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Identification of the analytes is based on liquid chromatographic retention times of analytes and two fragment ions produced by a triple quadrupole mass spectrometer. Quantification is based on electrospray ionization (ESI), and multiple reaction monitoring (MRM) was also utilized for getting better selectivity. The analytes and internal standard were separated from the urine matrix by solid-phase extraction (SPE). The method was applied for the determination of these compounds in urine samples of patients from surgical, medical and psychiatric wards. Of the dimethylated amines, only bufotenine was found in significant amounts (up to 34 microg/L). In keeping with our earlier results, the bufotenine excretion of psychiatric patients was found to be higher than that of the somatic patients. Method, procedure, considerations, statistical evaluations and urine sample spectra are presented.
Assuntos
Bufotenina/urina , Cromatografia Líquida de Alta Pressão/métodos , Alucinógenos/urina , Metoxidimetiltriptaminas/urina , N,N-Dimetiltriptamina/urina , Espectrometria de Massas por Ionização por Electrospray/métodos , Triptaminas/urina , Humanos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
The use of liquid chromatography with on-line fluorescence detection has formed the basis for the separation, characterisation and quantitation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine formed both in vitro and in vivo. Verification of the identity of metabolites has previously been facilitated by the combined use of a number of analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. We now describe the combination of liquid chromatography with gas chromatography-mass spectrometry for the unequivocal verification of a number of structurally characteristic metabolites of the psychotomimetic indolealkylamines.